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    2025 Vol. 24 No. 6 Previous Issue   

    Commentary
    Crop Science
    Horticulture
    Plant Protection
    Animal Science · Veterinary Medicine
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    Commentary
    Managing forage for grain: Strategies and mechanisms for enhancing forage production to ensure feed grain security
    Ruixuan Xu, Zheng Pu, Shuangxin Han, Hongqian Yu, Chuan Guo, Qingsheng Huang, Yingjun Zhang
    2025, 24(6): 2025-2034.  DOI: 10.1016/j.jia.2024.11.017
    Abstract ( )   PDF in ScienceDirect  

    The “Greater Food” approach has replaced the older “taking grain production as a top priority” approach.  The importance of feed and forage as the material basis for guaranteeing high-quality development of the livestock industry has gradually become prominent.  However, owing to the tradition of “both human staple food and animal feed relying on grain production” in China and the decoupling of feed crop planting and livestock farming, the risk of feed grain security has increased, especially as it relates to the supply of high-quality protein feed ingredients from abroad, which is facing a bottleneck.  To ensure food security, effective domestic agricultural production should be adopted.  Nevertheless, guaranteeing the supply of high-quality protein feed through domestic soybean production is difficult because of limited arable land; furthermore, pressure on the staple food supply is still extreme.  In this article, the historical and realistic implications for the security risks of feed grain in China are analyzed.  Proposals are made to separate staple food grains for humans from the feed grain supply for animals and to develop high-quality forage to reduce feed grain use.  High-quality forage can be supplied via intercropping with grain crops in arable land and reseeding perennial legumes or grasses into natural grasslands.  However, “managing forage for grain” needs to be supported via technical paths and policies as the forage industry develops to effectively increase the capacity to ensure feed grain security.


    Crop Science
    OsEXO70L2 is required for large lateral root formation and arbuscular mycorrhiza establishment in rice
    Tongming Wang, Kai Zhou, Bingxian Yang, Benoit Lefebvre, Guanghua He
    2025, 24(6): 2035-2045.  DOI: 10.1016/j.jia.2024.04.007
    Abstract ( )   PDF in ScienceDirect  

    As a major subunit of the exocyst complex, members of the EXO70 family have mainly been shown to play roles in cell polarity and morphogenesis in Arabidopsis, but their roles in plant endosymbiosis, such as with arbuscular mycorrhizal fungi (AMF), have rarely been reported.  Here, using knockout and overexpression lines, we show that OsEXO70L2, which encodes a divergent EXO70 protein in rice, controls the number of primary roots and is essential for large lateral root formation.  Furthermore, the OsEXO70L2 mutant sr1 displayed rare internal AMF hyphae and no arbuscules.  We also found that AMF sporulation can occur in roots despite low colonization and that AMF colonization and sporulation are modulated by photoperiod and co-culture with clover.  Finally, genes related to auxin homeostasis were found to be affected in the OsEXO70L2 knockout or overexpression lines, suggesting that auxin is at least partly responsible for the phenotypes.  This study provides new perspectives on the role of the exocyst complex during root development and AM in rice.

    GWAS analysis reveals candidate genes associated with density tolerance (ear leaf structure) in maize (Zea mays L.)
    Chunxiang Li, Yongfeng Song, Yong Zhu, Mengna Cao, Xiao Han, Jinsheng Fan, Zhichao Lü, Yan Xu, Yu Zhou, Xing Zeng, Lin Zhang, Ling Dong, Dequan Sun, Zhenhua Wang, Hong Di
    2025, 24(6): 2046-2062.  DOI: 10.1016/j.jia.2024.01.023
    Abstract ( )   PDF in ScienceDirect  

    Planting density is a major limiting factor for maize yield, and breeding for density tolerance has become an urgent issue.  The leaf structure of the maize ear leaf is the main factor that restricts planting density and yield components.  In this study, a natural population of 201 maize inbred lines was used for genome-wide association analysis, which identified nine SNPs on chromosomes 2, 5, 8, 9, and 10 that were significantly associated with ear leaf type structure.  Further verification through qRT-PCR confirmed the association of five candidate genes with these SNPs, with the Zm00001d008651 gene showing significant differential expression in the compact and flat maize inbred lines.  Enrichment analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) databases suggested that this gene is involved in the glycolysis process.  An analysis of the basic properties of this gene revealed that it encodes a stable, basic protein consisting of 593 amino acids with some hydrophobic properties.  The promoter region contains stress and hormone (abscisic acid (ABA)) related elements.  The mutant of this gene increased the first ear leaf angle (eLA) and leaf angle of the first leaf below the first ear (bLA) by 4.96 and 0.97°, respectively, compared with normal inbred lines.  Overall, this research sheds light on the regulatory mechanism of ear and leaf structures that influence density tolerance and provides solid foundational work for the development of new varieties.

    Identification of genomic regions and candidate genes underlying carotenoid accumulation in soybean using next-generation sequen-cing based bulk segregant analysis
    Berhane S. Gebregziabher, Shengrui Zhang, Jing Li, Bin Li, Junming Sun
    2025, 24(6): 2063-2079.  DOI: 10.1016/j.jia.2024.02.005
    Abstract ( )   PDF in ScienceDirect  

    The improvement of soybean seed carotenoid contents is very important due to the beneficial role of carotenoids in human health and nutrition.  However, the genetic architecture underlying soybean carotenoid biosynthesis remains largely unknown.  In the present study, we employed next generation sequencing-based bulked-segregant analysis to identify new genomic regions governing seed carotenoids in 1,551 natural soybean accessions.  The genomic DNA samples of individual plants with extreme phenotypes were pooled to form two bulks with high (50 accessions) and low (50 accessions) carotenoid contents for Illumina sequencing.  A total of 125.09 Gb of clean bases and 89.82% of Q30 were obtained, and the average alignment efficiency was 99.45% with an average coverage depth of 62.20× and 99.75% genome coverage.  Based on the G prime statistic algorithm (G´) method analysis, 16 candidate genomic loci with a total length 20.41 Mb were found to be related to the trait.  Of these loci, the most significant regions displaying the highest elevated G´ values were found in chromosome 06 at a position of 18.53–22.67 Mb, and chromosome 19 at genomic region intervals of 8.36–10.94, 12.06–13.79 and 18.45–20.26 Mb.  These regions were then used to identify the key candidate genes.  In these regions, 250 predicted genes were found and analyzed to obtain 90 significantly enriched (P<0.05) Gene Ontology (GO) terms.  Based on ANNOVAR analysis, 50 genes with non-synonymous and stopgained mutations were preferentially selected as potential candidate genes.  Of those 50 genes, following their gene annotation functions and high significant haplotype variations in various environments, five genes were identified as the most promising candidate genes regulating soybean seed carotenoid accumulation, and they should be investigated in further functional validation studies.   Collectively, understanding the genetic basis of carotenoid pigments and identifying genes underpinning carotenoid accumulation via a bulked-segregant analysis-based sequencing (BSA-seq) approach provide new insights for exploring future molecular breeding efforts to produce soybean cultivars with high carotenoid content.


    ORF138 causes abnormal lipid metabolism in the tapetum that leads to Ogu cytoplasmic male sterility in Brassica napus
    Xiaoyu Ge, Junlin Chen, Ouqi Li, Min Zou, Baolong Tao, Lun Zhao, Jing Wen, Bin Yi, Jinxing Tu, Jinxiong Shen
    2025, 24(6): 2080-2095.  DOI: 10.1016/j.jia.2024.03.009
    Abstract ( )   PDF in ScienceDirect  

    Mutations and rearrangements of mitochondrial genes cause plant cytoplasmic male sterility.   It is a significant way to utilize hybrid vigor to enhance crop yield.  Ogu cytoplasmic male sterility (CMS) is a natural cytoplasmic male sterility type discovered in radishes, being successfully transferred to rapeseed and cruciferous vegetables.  However, current studies lack depth in exploring the molecular mechanisms of its male sterility.  This study confirmed that orf138 is the causal gene for Ogu CMS through the genetic transformation in Arabidopsis.  Transcriptome analysis of aborted anthers in different stages suggested that differentially expressed genes (DEGs) are mainly enriched in pathways such as glycerophospholipid metabolism and arginine and proline metabolism.  It reveals that key genes involved in lipid metabolism pathways are significantly down-regulated in the sterile line (OguA), including BnaGPAT1, localized within the tapetum mitochondrial and endoplasmic reticulum.  This could lead to changes in the metabolism of substances like acylglycerols within the tapetum, causing disruptions in lipid metabolism.  This is consistent with morphological and subcellular structural changes in the tapetum and microspore cells, as observed in the transmission electron microscopy.  This abnormal lipid metabolism may trigger specific reactive oxygen species (ROS) accumulation in an oxidative stress response, ultimately leading to an aborted microspore.  Our study based on transcriptome has deepened our understanding of the molecular mechanisms in Ogu CMS.

    Identification of oil content QTLs on Arahy12 and Arahy16, and development of KASP markers in cultivated peanut (Arachis hypogaea L.)
    Bingyan Huang, Hua Liu, Yuanjin Fang, Lijuan Miao, Li Qin, Ziqi Sun, Feiyan Qi, Lei Chen, Fengye Zhang, Shuanzhu Li, Qinghuan Zheng, Lei Shi, Jihua Wu, Wenzhao Dong, Xinyou Zhang
    2025, 24(6): 2096-2105.  DOI: 10.1016/j.jia.2023.11.010
    Abstract ( )   PDF in ScienceDirect  

    Peanut kernels rich in oil, particularly those with oleic acid as their primary fatty acid, are in high demand among consumers, the food industry, and farmers due to their superior nutritional content, extended shelf life, and health benefits.  The oil content and fatty acid composition are governed by multiple genetic factors.  Identifying the quantitative trait loci (QTLs) related to these attributes will facilitate marker-assisted selection and genomic selection, thus enhancing quality-focused peanut breeding programs.  For this purpose, we developed a population of 521 recombinant inbred lines (RILs) and tested their kernel quality traits across five different environments.  We identified two major and stable QTLs for oil content, qOCAh12.1 and qOCAh16.1.  The markers linked to these QTLs were designed by Kompetitive allele-specific PCR (KASP) and subsequently validated.  Moreover, we found that the superior haplotype of oil content in the qOCAh16.1 region was conserved within the plant introduction (PI) germplasm cluster, as evidenced by a diverse peanut accession panel.  In addition, we determined that qAh09 and qAh19.1, which harbor the key gene encoding fatty acid desaturase 2 (FAD2), influence all seven fatty acids, palmitic, stearic, oleic, linoleic, arachidic, gadoleic, and behenic acids.  Regarding the protein content and the long-chain saturated fatty acid behenic acid, qAh07 emerged as the major and stable QTL, accounting for over 10% of the phenotypic variation explained (PVE).  These findings can enhance marker-assisted selection in peanut breeding, with the aim of improving the oil content, and deepen our understanding of the genetic mechanisms that shape fatty acid composition. 

    Post-anthesis dry matter production and leaf nitrogen distribution are associated with root-derived cytokinins gradient in rice
    Kuanyu Zhu, Yuemei Xu, Zhiwei Sun, Yajun Zhang, Weiyang Zhang, Yunji Xu, Junfei Gu, Hao Zhang, Zhiqin Wang, Lijun Liu, Jianhua Zhang, Jianchang Yang
    2025, 24(6): 2106-2122.  DOI: 10.1016/j.jia.2024.02.010
    Abstract ( )   PDF in ScienceDirect  

    Aligning leaf nitrogen (N) distribution to match the light gradient is crucial for maximizing canopy dry matter production (DMP) and improving N utilization efficiency.  However, the relationship between the gradient of root-derived cytokinins and N distribution in rice leaves and its impact on DMP and the underlying mechanisms remains poorly understood.  A two-year field experiment was conducted using two japonica N-efficient varieties (NEVs) and two japonica N-inefficient varieties (NIVs) under four different N rates (0, 90, 180, and 360 kg N ha−1).  These selected varieties exhibited similar values in the coefficient of light extinction (KL).  Results showed that at lower N rates (0–180 kg N ha−1), the NEVs exhibited greater dry matter weight at maturity, higher grain yield, and improved internal N use efficiency (IEN) compared to the NIVs, despite possessing comparable total N uptake.  Compared with the NIVs, the NEVs exhibited a more pronounced nitrogen distribution gradient in leaves, as indicated by the coefficient of nitrogen extinction (KN) values during the middle and early grain-filling stages.  This enhanced gradient led to improved coordination between light and nitrogen, resulting in greater photosynthetic production, particularly at lower N rates.  Furthermore, the NEVs demonstrated a larger gradient of zeatin (Z)+zeatin riboside (ZR) in leaves (i.e., higher ratios of Z+ZR levels between upper and lower leaves), enhanced expression levels of genes related to N export in lower leaves and Z+ZR loading in the root, respectively, elevated enzymes activities related to N assimilation in upper leaves, in relative to the NIVs.  Correlation and random forest analyses demonstrated a strong positive correlation between the Z+ZR gradient, KN, and DMP, and the gradient facilitated the export of N from lower leaves and its assimilation in upper leaves, contributing significantly to both KN and DMP.  This process was closely linked to root activity, including root oxidation activity, root Z+ZR content, and Z+ZR loading capacity, as confirmed by applying an inhibitor or a promoter of cytokinins biosynthesis to roots.  Interestingly, at the N rate of 360 kg N ha−1, both NEVs and NIVs showed indistinguishable plant traits, achieving a super high-yielding level (over 10.5 t ha−1) but with remarkably low IEN.  The results suggest that increasing the Z+ZR gradient can improve KN and DMP, where it needs to maintain higher root activity, thus leading to high yield and high IEN.  Further research is needed to explore and develop cultivation practices with reduced N to unlock the super-high-yielding potential of the NEVs.

    The coupled effects of various irrigation schedules and split nitrogen fertilization modes on post-anthesis grain weight variation, yield, and grain quality of drip-irrigated winter wheat (Triticum aestivum L.) in the North China Plain
    Abdoul Kader Mounkaila Hamani, Sunusi Amin Abubakar, Yuanyuan Fu, Djifa Fidele Kpalari, Guangshuai Wang, Aiwang Duan, Yang Gao, Xiaotang Ju
    2025, 24(6): 2123-2137.  DOI: 10.1016/j.jia.2023.12.037
    Abstract ( )   PDF in ScienceDirect  

    Irrigation methods and nitrogen (N) fertilization modes have complicated impacts on wheat physiology, growth, and development, leading to the regulation of wheat grain yield and quality.  However, the optimal water-N combination for drip-irrigated winter wheat remains unclear.  A two-year field study was conducted to evaluate the influences of various N-fertigation and water regimes on wheat post-anthesis grain weight variation, yield, grain NPK content, and grain quality.  The two irrigation quotas were I45 (irrigation when crop evapotranspiration reduced by effective rainfall (ETa-P) reaches 45 mm) and I30 (irrigation when ETa-P reaches 30 mm), while the six N application rates were N0–100 (100% at jointing/booting), N25–75 (25% at sowing and 75% at jointing/booting), N50–50 (50% at sowing and 50% at jointing/booting), N75–25 (75% at sowing and 25% at jointing/booting), N100–0 (100% at sowing), and SRF100 (100% of slow-release fertilizer at sowing).  The experimental findings showed that post-anthesis grain weight variation, grain yield, grain NPK content, and grain quality were all markedly influenced by the various irrigation schedules and N-fertilization modes.  The N50–50 treatment was more beneficial for winter wheat post-anthesis grain weight variation than the N100–0 and N0–100 treatments under the two irrigation quotas and during the two seasons.  The highest grain yields of 9.72 and 9.94 (t ha−1) were obtained with the I45N50–50 treatment in 2020–2021 and 2021–2022, respectively.  The grain crude protein was higher in the I45SRF100 treatment during the two seasons.  The I45N100–0 combination significantly (P<0.05) enhanced the content of grain total starch by 7.30 and 8.23% compared with the I45N0–100 and I30N0–100 treatments, respectively, during the 2021–2021 season.  The I45N100–0 treatment significantly (P<0.05) enhanced the content of grain total starch concentration by 7.77, 7.62 and 7.88% compared with the I45N0–100, I30N0–100, and I30N25–75 treatments, respectively, in the 2021–2022 season.  The principal component analysis (PCA) indicated that the N50–50 split N-fertigation mode could be the optimal choice for farmers during winter wheat production via drip irrigation.  

    A suitable organic fertilizer substitution ratio stabilizes rainfed maize yields and reduces gaseous nitrogen loss in the Loess Plateau, China
    Lihua Xie, Lingling Li, Junhong Xie, Jinbin Wang, Zechariah Effah, Setor Kwami Fudjoe, Muhammad Zahid Mumtaz
    2025, 24(6): 2138-2154.  DOI: 10.1016/j.jia.2024.03.021
    Abstract ( )   PDF in ScienceDirect  

    The application of organic fertilizers has become an increasingly popular practice in maize production to reduce the gaseous nitrogen (N) loss and soil degradation caused by inorganic fertilizers.  Organic fertilizer plays a key role in improving soil quality and stabilizing maize yields, but few studies have compared different substitution rates.  A field study was carried out in 2021 and 2022, based on a long-term trial initiated in 2016, which included five organic fertilizer N substitution rates with equal inputs of 200 kg N ha–1: 0% organic fertilizer (T1, 100% inorganic fertilizer), 50.0% organic+50.0% inorganic fertilizer (T2), 37.5% organic+62.5% inorganic fertilizer (T3), 25.0% organic+75.0% inorganic fertilizer (T4), and 12.5% organic+87.5% inorganic fertilizer (T5), as well as a no fertilizer control (T6).  The results of the two years showed that T3 and T1 had the highest grain yield and biomass, respectively, and there was no significant difference between T1 and T3.  Compared with T1, the 12.5, 25.0, 37.5, and 50.0% substitution rates in T5, T4, T3, and T2 significantly reduced total nitrogen losses (NH3, N2O) by 8.3, 16.1, 18.7, and 27.0%, respectively.  Nitrogen use efficiency (NUE) was higher in T5, T3, and T1, and there were no significant differences among them.  Organic fertilizer substitution directly reduced NH3 volatilization and N2O emission from farmland by lowering the ammonium nitrogen and alkali-dissolved N contents and by increasing soil moisture.  These substitution treatments reduced N2O emissions indirectly by regulating the abundances of AOB and nirK-harboring genes by promoting soil moisture.  Specifically, the 37.5% organic fertilizer substitution reduces NH3 volatilization and N2O emission from farmland by reducing the ammonium nitrogen and alkali-dissolved N contents and increasing moisture, which negatively regulate the abundance of AOB and nirK-harboring genes to reduce N2O emissions indirectly in rainfed maize fields on the Loess Plateau of China.

    Horticulture
    The circadian clock shapes diurnal gene expression patterns linked to glucose metabolic processes in Chinese cabbage
    Shan Wang, Kailin Shi, Yufan Xiao, Wei Ma, Yiguo Hong, Daling Feng, Jianjun Zhao
    2025, 24(6): 2155-2170.  DOI: 10.1016/j.jia.2024.08.026
    Abstract ( )   PDF in ScienceDirect  

    The plant circadian clock temporally drives gene expression throughout the day and coordinates various physiological processes with diurnal environmental changes.  It is essential for conferring plant fitness and competitive advantages to survive and thrive under natural conditions through the circadian control of gene transcription.  Chinese cabbage (Brassica rapa ssp. pekinensis) is an economically important vegetable crop worldwide, although there is little information concerning its circadian clock system.  Here we found that gene expression patterns are affected by circadian oscillators at both the transcriptional and post-transcriptional levels in Chinese cabbage.  Time-course RNA-seq analyses were conducted on two short-period lines (SPcc-1 and SPcc-2) and two long-period lines (LPcc-1 and LPcc-2) under constant light.  The results showed that 32.7–50.5% of the genes were regulated by the circadian oscillator and the expression peaks of cycling genes appeared earlier in short-period lines than long-period lines.  In addition, approximately 250 splicing events exhibited circadian regulation, with intron retention (IR) accounting for a large proportion.  Rhythmically spliced genes included the clock genes LATE ELONGATED HYPOCOTYL (BrLHY), REVEILLE 2 (BrRVE2) and EARLY FLOWERING 3 (BrELF3).  We also found that the circadian oscillator could notably influence the diurnal expression patterns of genes that are associated with glucose metabolism via photosynthesis, the Calvin cycle and the tricarboxylic acid (TCA) cycle at both the transcriptional and post-transcriptional levels.  The collective results of this study demonstrate that circadian-regulated physiological processes contribute to Chinese cabbage growth and development.


    Construction of a high-density genetic map to explore the genetic regulation of erucic acid, oleic acid, and linolenic acid contents in Brassica juncea
    Wei Yan, Jinze Zhang, Yingfen Jiang, Kunjiang Yu, Qian Wang, Xu Yang, Lijing Xiao, Entang Tian
    2025, 24(6): 2171-2189.  DOI: 10.1016/j.jia.2024.11.028
    Abstract ( )   PDF in ScienceDirect  

    Rapeseed mustard (Brassica juncea L.) is the third most important oilseed crop in the world, but the genetic mechanism underlying its massive phenotypic variation remains largely unexplored.  In this study, specific length amplified fragment sequencing (SLAF-Seq) was used to resequence a population comprising 197 F8 recombinant inbred lines (RILs) derived from a cross between vegetable-type Qichi881 and oilseed-type YufengZC of Bjuncea.  In total, 438,895 high-quality SLAFs were discovered, 47,644 of which were polymorphic, and 3,887 of the polymorphic markers met the requirements for genetic map construction.  The final map included 3,887 markers on 18 linkage groups and was 1,830.23 centiMorgan (cM) in length, with an average distance of 0.47 cM between adjacent markers.  Using the newly constructed high-density genetic map, a total of 53 QTLs for erucic acid (EA), oleic acid (OA), and linolenic acid (LNA) were detected and integrated into eight consensus QTLs with two for each of these traits.  For each of these three traits, two candidate genes were cloned and sequence analysis indicated colocalization with their respective consensus QTLs.  The co-dominant allele-specific markers for Bju.FAD3.A03 and Bju.FAD3.B07 were developed and showed co-localization with their consensus QTLs and co-segregation with LNA content, further supporting the results of QTL mapping and bioinformatic analysis.  The expression levels of the cloned homologous genes were also determined, and the genes were tightly correlated with the EA, OA and LNA contents of different lines.  The results of this study will facilitate the improvement of fatty acid traits and molecular breeding of Bjuncea.  Further uses of the high-density genetic map created in this study are also discussed. 


    ZF protein C2H2-71 regulates the soluble solids content in tomato by inhibiting LIN5
    Fangman Li, Junshen Lin, John Kojo Ahiakpa, Wenxian Gai, Jinbao Tao, Pingfei Ge, Xingyu Zhang, Yizhuo Mu, Jie Ye, Yuyang Zhang
    2025, 24(6): 2190-2202.  DOI: 10.1016/j.jia.2024.11.027
    Abstract ( )   PDF in ScienceDirect  

    Soluble solids content (SSC) plays an important role in determining the flavor of tomato fruits.  Tomato fruit SSC is transcriptionally regulated via sugar metabolism.  Previous studies have predominantly focused on the role of C2H2-type zinc finger proteins in tomato growth and development.  However, the specific regulatory mechanisms of C2H2 in the accumulation of soluble solids in tomato fruits are not fully understood.  This study used eight tomato accessions with varying levels of SSC to study the expression of SlC2H2 family genes in red ripe fruits.  The levels of SlC2H2-71 expression were found to be significantly reduced in high-SSC accessions compared to low-SSC accessions.  Several Slc2h2-71 mutant lines were developed using the CRISPR-Cas9 system, which led to elevated levels of soluble solids, fructose, glucose, malic acid, and citric acid in mature red ripe fruits.  However, the sucrose content in the edited Slc2h2-71 mutant lines was generally lower.  RNA-seq analysis revealed that fruits from the mutant lines had altered expression of genes related to the sugar and acid metabolic pathways, which was further confirmed by quantitative real-time PCR.  Specifically, the expression of SlLIN5 encoding the cell wall invertase (CWIN) was elevated.  The yeast one-hybrid (Y1H) assay, 35S::UAS-GUS, dual-luciferase reporter systems and electrophoretic mobility shift assay (EMSA) demonstrated that C2H2-71 regulates tomato sugar metabolism by directly binding to the promoter region of SlLIN5, culminating in the repression of its transcriptional activity.  The activity of acid invertase exhibited a significantly higher level in the SlC2H2-71 knock-out lines compared to the control lines.  In summary, the regulation of tomato fruit SSC by C2H2-71 involves the inhibition of SlLIN5 expression.

    Introgressed eggplant lines with the wild Solanum incanum evaluated under drought stress conditions
    Martín Flores-Saavedra, Pietro Gramazio, Santiago Vilanova, Diana M. Mircea, Mario X. Ruiz-González, Óscar Vicente, Jaime Prohens, Mariola Plazas
    2025, 24(6): 2203-2216.  DOI: 10.1016/j.jia.2024.03.014
    Abstract ( )   PDF in ScienceDirect  

    As access to irrigation water becomes increasingly limited, introgression of relevant genomic regions from drought-tolerant wild genotypes is a promising breeding strategy for crop plants.  In this study, nine eggplant (Solanum melongena) introgression lines (ILs) covering altogether 71.6% of the genome of the donor wild relative parent S. incanum were evaluated for drought tolerance under water stress conditions.  Plants at the five true leaves stage were irrigated at either 100% (control) or 30% (water stress) field capacity for 14 days, and growth and biochemical traits were measured.  Reduced irrigation resulted in decreased growth and increased stress markers such as proline and malondialdehyde.  Most ILs had lower growth and biomass production than the cultivated parent under both conditions.  However, the wild alleles for two genomic regions related to stem and root dry weight conferred improved tolerance to water stress.  In addition, several S. incanum alleles had a positive effect on important traits that may improve yield under drought conditions, such as leaf water content water use efficiency, and chlorophyll content.  Fine-mapping the genomic regions for tolerance and reducing linkage drag with regions affecting growth will be crucial for significantly improving eggplant drought tolerance through introgression breeding.


    Transcriptome-based analysis of lignin accumulation in the regulation of fruit stone development and endocarp hardening in Chinese jujube
    Xinyi Mao, Xuan Zhao, Zhi Luo, Ao He, Meng Yang, Mengjun Liu, Jin Zhao, Ping Liu
    2025, 24(6): 2217-2228.  DOI: 10.1016/j.jia.2024.12.032
    Abstract ( )   PDF in ScienceDirect  

    Stone fruits, also known as drupes, have evolved an extremely hard wood-like shell called a stone to protect the seeds.  Recently, the market value of stoneless cultivars has risen dramatically, which highlights the need to cultivate stoneless fruit.  Therefore, determining the underlying mechanism of fruit stone development is urgently needed.  By employing the stone-containing jujube cultivar ‘Youhe’ and two stoneless Chinese jujube cultivars, ‘Wuhefeng’ and ‘Daguowuhe’, we comprehensively studied the mechanism of fruit stone development in jujube.  Anatomical analysis and lignin staining revealed that the stone cultivar ‘Youhe’ jujube exhibited much greater lignin accumulation in the endocarp than the two stoneless cultivars.  Lignin accumulation may be the key factor in fruit stone formation.  By analyzing the transcriptome data and identifying differentially expressed genes (DEGs), 49 overlapping DEGs were identified in the comparisons of ‘Youhe’ jujube vs. ‘Wuhefeng’ jujube and ‘Youhe’ jujube vs. ‘Daguowuhe’ jujube.  ZjF6H1-3 and ZjPOD, which are involved in lignin synthesis, were identified among these DEGs.  The overexpression and silencing of ZjF6H1-3 and ZjPOD in wild jujube seedlings further confirmed their roles in lignin synthesis.  In addition, two bHLH transcription factors were included in the 49 overlapping DEGs, and bHLH transcription factor binding motifs were found in the promoters of ZjF6H1-3 and ZjPOD, indicating that bHLH transcription factors are also involved in lignin synthesis and stone formation in Chinese jujube.  This study provides new insights into the molecular networks underlying fruit stone formation and can serve as an important reference for the molecular design and breeding of stoneless fruit cultivars of jujube and fruit trees.

    MdERF2 regulates cuticle wax formation by directly activating MdLACS2, MdCER1 and MdCER6 of apple fruit during postharvest
    Xinyue Zhang, Xinhua Zhang, Wenwen Sun, Meng Lv, Yefei Gu, Sarfaraz Hussain, Xiaoan Li, Maratab Ali, Fujun Li
    2025, 24(6): 2229-2239.  DOI: 10.1016/j.jia.2024.11.033
    Abstract ( )   PDF in ScienceDirect  

    Ethylene response factors 2 (ERF2) are essential for plant growth, fruit ripening, metabolism, and resistance to stress.  In this study, the expression levels of the genes for MdERF2 implicated in the biosynthesis, composition and ultrastructure of fruit cuticular wax in apple (Malus domestica) were studied by the transfection of apple fruit and/or calli with MdERF2-overexpression (ERF2-OE) and MdERF2-interference (ERF2-AN) vectors.  In addition, the direct target genes of MdERF2 related to wax biosynthesis were identified using electrophoretic mobility shift assays (EMSAs) and dual-luciferase reporter (DLR) assays.  The findings indicated that the expression levels of four wax biosynthetic genes, long-chain acyl-CoA synthetase 2 (MdLACS2), eceriferum 1 (MdCER1), eceriferum 4 (MdCER4), and eceriferum 6 (MdCER6), were upregulated by ERF2-OE.  In contrast, the expression levels of these genes were inhibited when MdERF2 was silenced.  Furthermore, the overall structure and accumulation of fruit cuticular wax were influenced by the expression level of MdERF2.  Treatment with ERF2-OE significantly increased the proportions of alkanes and ketones and reduced the proportions of fatty acids and esters.  In addition, the EMSAs and DLR assays demonstrated that MdERF2 could bind directly to GCC-box elements in the promoters of MdLACS2, MdCER1, and MdCER6 to activate their transcription.  These results confirmed that MdERF2 targets the up-regulation of expression of the MdLACS2, MdCER1, and MdCER6 genes, thereby altering the composition, content, and microstructure of apple epidermal wax.


    QTL detection and candidate gene analysis of the anthracnose resistance locus in tea plant (Camellia sinensis)
    Chenyu Zhang, Hongli Li, Piao Mei, Yuanyuan Ye, Dingding Liu, Yang Gong, Haoran Liu, Mingzhe Yao, Chunlei Ma
    2025, 24(6): 2240-2250.  DOI: 10.1016/j.jia.2025.02.015
    Abstract ( )   PDF in ScienceDirect  

    Anthracnose is a devastating disease caused by Colletotrichum that significantly affects the yield and economic value of the tea plant (Camellia sinensis).  However, few studies have addressed the genetic mechanism of anthracnose resistance (AR).  This study investigated the QTL associated with AR in a ‘Longjing 43’בBaijiguan’ (LJ43×BJG) population.  The field surveys conducted in this study led to the identification of several QTLs for AR on the linkage map.  One major QTL (qAR-12.4) accounted for 12% of the phenotypic variance explained over two years.  The BSA-seq results also revealed two genomic regions, qARChr1 on chromosome 1 and qARChr13 on chromosome 13, which showed strong correlations with AR.  Time-course RNA-seq was performed on LJ43 and BJG inoculated with anthracnose at 0, 24, and 48 hours to screen for candidate genes.  The results showed the gradual post-inoculation expression of a nuclear-localized ERF transcription factor (CsERF105) within the qARChr1 locus in BJG but not in LJ43.  The AR of BJG was significantly reduced after feeding with CsERF105-specific antisense oligonucleotides, suggesting that CsERF105 may be a positive regulator.  The findings of this study add to our general knowledge of the genetic factors involved in the tea plant’s AR and potential breeding targets.


    Plant Protection
    Molecular evidence of the west-to-east dispersal of Puccinia striiformis f. sp. tritici in central Shaanxi and the migration of the inoculum from Gansu
    Wei Liu, Xueling Huang, Meng Ju, Mudi Sun, Zhimin Du, Zhensheng Kang, Jie Zhao
    2025, 24(6): 2251-2265.  DOI: 10.1016/j.jia.2023.10.026
    Abstract ( )   PDF in ScienceDirect  

    Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is an airborne disease.  In China, it frequently develops initially in central Shaanxi and southwestern Gansu, and from there, inoculum spreads to the eastern wheat production regions.  Field investigations have suggested that Pst could spread from the west to the east within central Shaanxi and that Gansu could serve as the inoculum source for central Shaanxi, but there is no direct evidence for this hypothetical dispersal route.  In the current study, 321 Pst isolates collected from central Shaanxi and Gansu in the 2019–2020 and 2020–2021 winter wheat cropping seasons were genotyped using 23 pairs of KASP-SNP markers.  The dispersion among subpopulations was analyzed using several approaches, and overall, the populations were found to exhibit high levels of genetic diversity.  There was little genetic divergence (0.05>Fst>0) within central Shaanxi.  However, significant gene flow (Nm>4) driven by wind-oriented dispersal from west (Baoji) to east (Weinan) occurred.  There was also gene flow among the 4 Gansu subpopulations of Tianshui, Longnan, Pingliang, and Qingyang.  Migration of the pathogen occurred between central Shaanxi and Gansu.  Migration from Gansu to central Shaanxi was major compared with that from central Shaanxi to Gansu that was minor.  Genetic variation occurred among isolates, instead of among subpopulations and within isolates.  Linkage disequilibrium revealed that there was strong genetic recombination in the subpopulations from Gansu and central Shaanxi.  Therefore, the present study provides molecular evidence that Pst spread from west to east in central Shaanxi and showed that Gansu (especially Longnan and Tianshui) was one of the major origins of the pathogen inoculum of wheat stripe rust in central Shaanxi.  The results revealed the west-to-east transmission route of wheat stripe rust in central Shaanxi, being used to guide integrated management of the disease. 

    The N-mannosyltransferase MoAlg9 plays important roles in the development and pathogenicity of Magnaporthe oryzae
    Shulin Zhang, Yu Wang, Jinmei Hu, Xinyue Cui, Xiaoru Kang, Wei Zhao, Yuemin Pan
    2025, 24(6): 2266-2284.  DOI: 10.1016/j.jia.2023.10.027
    Abstract ( )   PDF in ScienceDirect  

    Magnaporthe oryzae is the causal agent of rice blast.  Glycosylation plays key roles in vegetative growth, development, and infection of Moryzae.  However, several glycosylation-related genes have not been characterized.  In this study, we identified a Glyco_transf_22 domain-containing protein, MoAlg9, and found that MoAlg9 is localized to the endoplasmic reticulum (ER).  Deletion of MoALG9 significantly affected conidial production, normal appressorium formation, responses to stressors, and pathogenicity of Moryzae.  We also found that the ΔMoalg9 mutant was defective in glycogen utilization, appressorial penetration, and invasive growth in host cells.  Moreover, we further demonstrated that MoALG9 regulates the transcription of several target genes involved in conidiation, appressorium formation, and cell wall integrity.  In addition, we found that the Glyco_transf_22 domain is essential for normal MoAlg9 function and localization.  We also provide evidence that MoAlg9 is involved in N-glycosylation pathway in Moryzae.  Taken together, these results show that MoAlg9 is important for conidiation, appressorium formation, maintenance of cell wall integrity, and the pathogenesis of Moryzae.

    Application of dsRNA of FgPMA1 for disease control on Fusarium graminearum
    Luoyu Wu, Furong Chen, Pengwei Wang, Chongjing Xu, Weidong Wen, Matthias Hahn, Mingguo Zhou, Yiping Hou
    2025, 24(6): 2285-2298.  DOI: 10.1016/j.jia.2023.11.046
    Abstract ( )   PDF in ScienceDirect  

    Fusarium graminearum is a fungal plant pathogen which causes Fusarium head blight (FHB), a devastating disease on cereal crops.  Here we report that FgPMA1 could be a new target to control FHB by the application of double-stranded RNA (dsRNA) of FgPMA1.  FgPMA1 was divided into 6 segments to generated RNA interference (RNAi) constructs (FgPMA1RNAi-1, -2, -3, -4, -5, and -6), and these constructs were transformed in Fgraminearum strain PH-1.  The expression of FgPMA1 reduced by 18.48, 33.48 and 56.93% in FgPMA1RNAi-1, FgPMA1RNAi-2 and FgPMA1RNAi-5, respectively.  FgPMA1RNAi-1, -2, and -5 mutants inhibited fungal development, including mycelium growth, mycelial morphology, asexual and sexual development, and toxin production.  The length of lesions on wheat leaves, wheat coleoptiles and wheat ears were shorter after infection with FgPMA1RNAi-1, -2, and -5 mutants than wild type PH-1.  These results showed that three segments (FgPMA1RNAi-1, -2, and -5) exhibited effective silencing effects.  After treatment with 25 ng µL–1 dsRNA of these segments in vitro, the growth rate of mycelium growth was significant decreased, mycelium became deformed with bulbous structure at the tip, and the mycelium lost the ability to produce conidia in Fgraminearum strain PH-1, Fusarium asiacitum strain 2021 and phenamacril-resistant strain YP-1.  After application of FgPMA1RNAi-1-dsRNA and FgPMA1RNAi-2-dsRNA to wheat ears, pathogenicity reduced 34.21–35.40%.

    Overexpression of TuABCC4 is associated with abamectin resistance in Tetranychus urticae Koch
    Mingmei Wu, Rui Dong, Yan Zhang, Haojie Liao, Tian Tian, Dandan Xu, Youjun Zhang, Zhaojiang Guo, Shaoli Wang
    2025, 24(6): 2299-2310.  DOI: 10.1016/j.jia.2024.07.020
    Abstract ( )   PDF in ScienceDirect  

    Pesticide resistance greatly limits control efficacy after the long-term application of pesticides.  The two-spotted spider mite, Tetranychus urticae Koch, is a notorious agricultural pest worldwide that is resistant to various pesticides, including abamectin.  While some studies of abamectin resistance have investigated target resistance related to glutamate-gated chloride channels (GluCls), studies on the metabolic resistance mechanisms are still limited.  In this study, we identified an ABCC subfamily gene, TuABCC4, that was overexpressed in resistant populations of Turticae, based on the analysis of previously obtained transcriptomic and RNA-seq data.  No consistent nonsynonymous mutations in the TuABCC4 gene were found between the susceptible and resistant populations, although TuABCC4 expression was significantly increased in all the resistant populations that were studied.  Synergistic experiments with the inhibitor verapamil and gene expression analysis of the susceptible and resistant populations confirmed the key role of TuABCC4 in abamectin resistance.  In addition, an increase in the expression of the TuABCC4 gene was shown by RNA interference and genetic association analysis to be closely related to the resistance of Turticae to abamectin.  In conclusion, overexpression of TuABCC4 was shown to be involved in abamectin resistance in Turticae.  These results can help us to better understand the molecular basis of pest resistance to abamectin.


    Development of a piggyBac transgenic system in Bactrocera dorsalis and its potential for research on olfactory molecular targets
    Jie Zhang, Qi Wang, Jinxi Yuan, Zhen Tian, Shanchun Yan, Wei Liu
    2025, 24(6): 2311-2326.  DOI: 10.1016/j.jia.2024.07.021
    Abstract ( )   PDF in ScienceDirect  


    Chemicals that modify pest behavior are developed to reduce crop damage by altering pest behavior, using specific genes within the olfactory system as molecular targets. The identification of these molecular targets in Bactrocera dorsalis, also known as the functional study of key olfactory genes, relies on CRISPR/Cas9-mediated gene knockout techniques.  However, these techniques face limitations when applied to lethal genes.  Transgenic technology offers a solution since it enables precise manipulation of gene expression in specific tissues or during certain developmental stages.  Consequently, this study developed a piggyBac-mediated transgenic system in Bdorsalis to investigate reporter gene expression in olfactory organs, and assessed the olfactory behavior and antennal electrophysiological responses in transgenic lines.  The goal was to assess the potential of this approach for future research on olfactory gene function.  A universally expressed housekeeping gene from the BdorActin family was identified using the developmental transcriptome dataset.  Its candidate promoter region (BdorActinA3a-1P–2k) was then cloned into the piggyBac plasmid.  We subsequently established two stable transgenic lines with specific TTAA insertion sites on chromosomes 4 and 5, consistent with the characteristics of piggyBac transposition.  The transgenic strains exhibited essentially normal survival, with hatchability and adult lifespan unaffected, although there were slight reductions in the emergence rate and oviposition capacity.  The fluorescent reporter has been successfully expressed in olfactory-related organs, such as the antennae, proboscis, maxillary palp, legs, external genitalia, and brain.  The antennal electrophysiological responses to representative chemicals in the transgenic lines were consistent with those of the wild type.  However, some olfactory-related behaviors, such as pheromone response and mating, were significantly affected in the transgenic lines.  These findings suggest that our system could potentially be applied in future olfactory research, such as driving the expression of exogenous elements that are effective in olfactory organs.  However, caution is advised regarding its impact when applied to some olfactory-related behavioral phenotypes.


    Animal Science · Veterinary Medicine
    PGC-mediated conservation strategies for germplasm resources of Rugao Yellow chicken and Shouguang chicken in China
    Guangzheng Liu, Wenjie Ren, Kai Jin, Dan Zheng, Qisheng Zuo, Yani Zhang, Guohong Chen, Bichun Li, Yingjie Niu
    2025, 24(6): 2327-2341.  DOI: 10.1016/j.jia.2024.05.019
    Abstract ( )   PDF in ScienceDirect  

    Germplasm resources are essential for the sustainable development of biodiversity and husbandry of local chickens, as well as for the breeding and industry of superior quality chickens.  Unfortunately, many local and indigenous chicken breeds are at risk of declining numbers, emphasizing the need to conserve breed resources for endangered chickens.  Primordial germ cells (PGCs) are crucial for preserving germplasm resources by inheriting genetic information from parents to offspring and ensuring stability of genetic material between germlines.  In this study, PGCs were isolated from chicken embryos’ gonads and cultured in FAcs medium without feeder cells.  Over a period of approximately 40 d, the cells proliferated to a number of up to 106, establishing various cell lines.  Particularly, 18 PGC lines were created from Rugao Yellow chicken and Shouguang chicken, with an efficiency ranging from 39.1 to 45%.  Furthermore, PGCs that had been cultured for 40 passages exhibited typical PGC characteristics, such as glycogen staining reaction, and expression of pluripotency and reproductive markers.  These results confirm that PGCs maintain stem cell properties even after long-term in vitro culture.  Additionally, PGCs cryopreserved for up to 120 d remained viable, maintained typical PGC morphologies, and possessed stable cell proliferation ability.  Through intravascular injection into chicken embryos, green fluorescent protein (GFP)-PGCs were found in the recipient embryos’ gonads and could develop into gametes to produce offspring, indicating that even after extended culture, PGCs retain their migratory and lineage-transmitting capabilities.  This research offers valuable insights into the in vitro cultivation and preservation of PGCs of Chinese indigenous chickens.  The findings of this study can be applied in transgenic chicken production and the preservation of genetic resources of indigenous chicken breeds.

    Evolution and biological characteristics of the circulated H8N4 avian influenza viruses
    Hong Zhang, Jing Guo, Peng Peng, Mengjing Wang, Jinyan Shen, Xiaohong Sun, Mengdi Guan, Pengfei Cui, Guohua Deng, Dong Chu, Xuyong Li
    2025, 24(6): 2342-2355.  DOI: 10.1016/j.jia.2023.12.033
    Abstract ( )   PDF in ScienceDirect  

    The circulating avian influenza viruses in wild birds have a high possibility of spillover into domestic birds or mammals at the wild bird–domestic bird or bird–mammal interface.  H8N4 viruses primarily circulate in migratory wild waterfowl and have rarely been identified in domestic birds.  In this study, we summarized the spatial and temporal distribution of global H8 viruses, specified their natural reservoirs, and performed detailed evolutionary analysis on the dominant H8N4 viruses.  Here, we also report a novel H8N4 virus isolated from a Eurasian coot sample from a wetland in eastern China in 2022.  Animal infection studies indicated that the wild bird-originated H8N4 virus can replicate and transmit efficiently in ducks but has not adapted to chickens.  Additionally, this naturally isolated H8N4 virus can replicate in mice without prior adaptation.  These results indicate that H8 viruses exist mainly in the wild duck reservoir and pose a high infection risk to domestic ducks.  Therefore, the active surveillance of influenza viruses at the wild and domestic waterfowl interface will contribute to monitoring the circulation of these viruses.


    Protection of chickens from Mycoplasma gallisepticum through the MAPK/ERK/JNK pathway by a compound of ten Chinese medicine formulas
    Tengfei Wang, Changyong Fan, Yufei Xiao, Shan Lü, Guangyang Jiang, Mengyun Zou, Yingjie Wang, Qiao Guo, Zhenghao Che, Xiuli Peng
    2025, 24(6): 2356-2370.  DOI: 10.1016/j.jia.2023.11.043
    Abstract ( )   PDF in ScienceDirect  

    Mycoplasma gallisepticum (MG) is a common avian pathogen that mainly infects poultry, causing significant reductions in body weight gain and egg production, along with damage to immune organs and immunosuppression.  MG is susceptible to co-infections with other pathogens, leading to increased mortality rates and significant economic losses in the global poultry industry.  While antibiotics have been extensively applied worldwide to treat MG infections in poultry production, concerns regarding antibiotic resistance and residue remain prevalent.  Traditional Chinese medicine (TCM), renowned for its natural, safe, and non-toxic properties, has shown significant anti-inflammatory and immune-enhancing effects.  This study aimed to investigate the protective effect of TCM on production performance and its impact on MG-induced immunosuppression through the MAPK/ERK/JNK signaling pathway in chickens.  Our results showed that TCM alleviated the negative effects of MG infection on production performance, as evidenced by improvements in body weight gain, feed conversion rate, survival rate, and immune organ index.  TCM exhibited direct inhibition of the MG proliferation in vitro and in vivo.  Furthermore, TCM treatment promoted the normalization of trachea and lung tissue structure in MG-infected chickens, leading to a significant reduction in inflammatory damage.  Moreover, following the treatment with the TCM, the production of pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) decreased significantly, accompanied by the downregulation of pro-apoptotic genes caspase3, caspase9, and BAX, both in vitro and in vivo.  A mechanism-based study showed that in vitro and in vivo treatment with the TCM significantly reduced the expression of key proteins, including early growth response gene 1 (EGR1), p-ERK, p-JNK, and p-JUN.  Altogether, TCM improved body weight gain, inhibited pro-inflammation responses, and alleviated tissue damage by inhibiting the MAPK/ERK/JNK signaling pathway to protect the performance and immune system of MG-infected chickens.

    Agro-ecosystem & Environment
    Long-term Chinese milk vetch incorporation promotes soil aggregate stability by affecting mineralogy and organic carbon
    Yulu Chen, Li Huang, Jusheng Gao, Zhen Zhou, Muhammad Mehran, Mingjian Geng, Yangbo He, Huimin Zhang, Jing Huang
    2025, 24(6): 2371-2388.  DOI: 10.1016/j.jia.2024.10.002
    Abstract ( )   PDF in ScienceDirect  

    Soil aggregates profoundly impact soil sustainability and crop productivity, and they are influenced by complex interactions between minerals and organics.  This study aimed to elucidate the alterations in mineralogy and soil organic carbon (SOC) following long-term green manure incorporation and the effect on soil aggregates.  Based on 5- and 36-year field experiments, surface soil samples (0–20 cm) were collected from Alfisol and Ferrisol soils subjected to rice–rice–winter fallow (CK) and rice–rice–Chinese milk vetch (MV) treatments to investigate aggregate stability, mineralogy, SOC composition, and soil microstructural characteristics.  The results showed that high clay-content Ferrisol exhibited greater aggregate stability than low clay-content Alfisol.  The phyllosilicates in Alfisol primarily comprised illite and vermiculite, whereas those in Ferrisol with high-content free-form Fe oxides (Fed) were dominated by kaolinite.  Additionally, the clay fraction in Ferrisol contained more aromatic-C than the clay fraction in Alfisol.  The 36-year MV incorporation significantly increased the Ferrisol macroaggregate stability (9.57–13.37%), and it also facilitated the transformation of vermiculite into kaolinite and significantly increased the clay, Fed, and aromatic-C contents in Ferrisol.  Backscattered electron (BSE)-scanning electron microscopy/energy dispersive X-ray spectroscopy (SEM/EDS) revealed a compact aggregate structure in Ferrisol with co-localization of Fe oxides and kaolinite.  Moreover, the partial least path model (PLS-PM) revealed that clay content directly improved macroaggregate stability, and that kaolinite and Fed positively and directly affected clay or indirectly modulated clay formation by increasing the aromatic-C levels.  Overall, long-term MV incorporation promotes clay aggregation by affecting mineral transformation to produce more kaolinite and Fe oxides and retain aromatic-C, and it ultimately improves aggregate stability.

    Optimal drip irrigation leaching amount and timing enhanced cotton fiber yield, quality and nitrogen uptake by regulating soil salinity and nitrate nitrogen in saline-alkaline fields
    Xiaoqiang Liu, Mingqi Li, Dong Xue, Shuai He, Junliang Fan, Fucang Zhang, Feihu Yin
    2025, 24(6): 2389-2409.  DOI: 10.1016/j.jia.2024.11.032
    Abstract ( )   PDF in ScienceDirect  

    Improving cotton fiber quality can increase the economic income of cotton farmers, but achieving high fiber quality without decreasing cotton fiber yield remains a major challenge in saline-alkaline cotton fields.  A field experiment was conducted in 2020 and 2021 on saline-alkaline soil with cotton under drip irrigation to examine how amount and timing of leaching affected soils salinity, cotton fiber yield and quality.  There were five leaching amounts (CK: 0 mm, W1: 75 mm, W2: 150 mm, W3: 225 mm and W4: 300 mm) and three leaching timings (T1: once at the seedling stage, T2: twice at the seedling and budding stages, and T3: thrice at the seedling, budding and pollen-setting stages).  Soil salinity, soil nitrate nitrogen (NO3-N), cotton nitrogen (N) uptake, irrigation water productivity (IWP), cotton fiber yield, fiber length, fiber uniformity, fiber strength, fiber elongation, micronaire and fiber quality index (FQI) were investigated.  The results indicated that soil salinity and NO3-N reduced with increasing leaching amount.  The N uptake of cotton bolls was greater than in cotton leaves, stems and roots, and total N accumulation increased with increasing leaching amount.  The optimal cotton fiber yield and IWP occurred in treatment W3T2, and were 3,199 and 2,771 kg ha−1, and 0.5482 and 0.4912 kg m−3 in 2020 and 2021, respectively.  Fiber length, strength, elongation, and uniformity increased with increasing leaching amount, while there was a negative relationship between fiber micronaire and leaching amount.  Soil salinity, NO3-N and fiber micronaire were negatively correlated with fiber quality (i.e., length, strength, elongation and uniformity) and yield, nitrogen uptake of various organs (i.e., root, stems and leaves) and whole plant nitrogen uptake.  Pearson correlation analysis revealed that fiber elongation was most sensitive to soil salinity.  The method of Entropy–Order Preference by Similarity to Ideal Solution (EM–TOPSIS) indicated that leaching of 300 mm of water applied equally at the seedling and budding periods was the optimal treatment to maintain soil salinity and nutrient levels and achieve high cotton fiber yield and quality.  In conclusion, the optimal level of leaching treatment decreased soil salinity and improved nitrogen uptake and was beneficial to achieve high fiber yield and quality.  Our results will be significant for guiding drip irrigation practice of leaching on saline-alkaline soils for sustainable cotton fiber production.


    Enhancing the yield and water use efficiency of processing tomatoes (Lycopersicon esculentum Miller) through optimal irrigation and salinity management under mulched drip irrigation
    Jiaying Ma, Jian Liu, Yue Wen, Zhanli Ma, Jinzhu Zhang, Feihu Yin, Tehseen Javed, Jihong Zhang, Zhenhua Wang
    2025, 24(6): 2410-2424.  DOI: 10.1016/j.jia.2025.03.021
    Abstract ( )   PDF in ScienceDirect  

    In recent years, the rational utilization of saline water resources for agricultural irrigation has emerged as an effective strategy to alleviate water scarcity.  To safely and efficiently exploit saline water resources over the long term, it is crucial to understand the effects of salinity on crops and develop optimal water-salinity irrigation strategies for processing tomatoes.  A two-year field experiment was conducted in 2018 and 2019 to explore the impact of water salinity levels (S1: 1 g L–1, S2: 3 g L–1, and S3: 5 g L–1) and irrigation amounts (W1: 305 mm, W2: 485 mm, and W3: 611 mm) on the soil volumetric water content and soil salinity, as well as processing tomato growth, yield, and water use efficiency.  The results showed that irrigation with low to moderately saline water (<3 g L–1) enhanced plant water uptake and utilization capacity, with the soil water content (SWC) reduced by 6.5–7.62% and 10.52–13.23% for the S1 and S2 levels, respectively, compared to the S3 level in 2018.  Under S1 condition, the soil salt content (SSC) accumulation rate gradually declined with an increase in the irrigation amount.  For example, W3 decreased by 85.00 and 77.94% compared with W1 and W2 in 2018, and by 82.60 and 73.68% in 2019, respectively.  Leaching effects were observed at the W3 level under S1, which gradually diminished with increasing water salinity and duration.  In 2019, the salt contents of soil under each of the treatments increased by 10.81–89.72% compared with the contents in 2018.  The yield of processing tomatoes increased with an increasing irrigation amount and peaked in the S1W3 treatment for the two years, reaching 125,304.85 kg ha–1 in 2018 and 128,329.71 kg ha–1 in 2019.  Notably, in the first year, the S2W3 treatment achieved relatively high yields, exhibiting only a 2.85% reduction compared to the S1W3 treatment.  However, the yield of the S2W3 treatment declined significantly in two years, and it was 15.88% less than that of the S1W3 treatment.  Structural equation modeling (SEM) revealed that soil environmental factors (SWC and SSC) directly influence yield while also exerting indirect impacts on the growth indicators of processing tomatoes (plant height, stem diameter, and leaf area index).  The TOPSIS method identified S1W3, S1W2, and S2W2 as the top three treatments.  The single-factor marginal effect function also revealed that irrigation water salinity contributed to the composite evaluation scores (CES) when it was below 0.96 g L–1.  Using brackish water with a salinity of 3 g L–1 at an irrigation amount of 485 mm over one year ensured that processing tomatoes maintained high yields with a relatively high CES (0.709).  However, using brackish water for more than one year proved unfeasible.

    The estimation method is the primary source of uncertainty in cropland nitrate leaching estimates in China
    Xingshuai Tian, Huitong Yu, Jiahui Cong, Yulong Yin, Kai He, Zihan Wang, Zhenling Cui
    2025, 24(6): 2425-2437.  DOI: 10.1016/j.jia.2024.08.023
    Abstract ( )   PDF in ScienceDirect  


    Cropland nitrate leaching is the major nitrogen (N) loss pathway, and it contributes significantly to water pollution.  However, cropland nitrate leaching estimates show great uncertainty due to variations in input datasets and estimation methods.  Here, we presented a re-evaluation of Chinese cropland nitrate leaching, and identified and quantified the sources of uncertainty by integrating three cropland area datasets, three N input datasets, and three estimation methods.  The results revealed that nitrate leaching from Chinese cropland averaged 6.7±0.6 Tg N yr−1 in 2010, ranging from 2.9 to 15.8 Tg N yr−1 across 27 different estimates.  The primary contributor to the uncertainty was the estimation method, accounting for 45.1%, followed by the interaction of N input dataset and estimation method at 24.4%.  The results of this study emphasize the need for adopting a robust estimation method and improving the compatibility between the estimation method and N input dataset to effectively reduce uncertainty.  This analysis provides valuable insights for accurately estimating cropland nitrate leaching and contributes to ongoing efforts that address water pollution concerns.


    Letter
    SiDWARF4 encodes an ent-copalyl diphosphate synthase (CPS) required for gibberellin biosynthesis and morphogenesis in foxtail millet
    Yongchao Li, Mengmeng Sun, Rui Zhao, Jiayi Chen, Yunhao Chen, Shuqi Dong, Xiangyang Yuan, Xiaorui Li, Lulu Gao, Guanghui Yang, Peiyong Xin, Shujing Cheng, Jinfang Chu, Xiaoqian Chu, Jiagang Wang
    2025, 24(6): 2438-2442.  DOI: 10.1016/j.jia.2024.12.002
    Abstract ( )   PDF in ScienceDirect  
    Preparation and characterization of monoclonal antibodies against the pp62 protein of African swine fever virus
    Zhiyong Xiang, Huan Ye, Peng Gao, Lei Zhou, Xinna Ge, Xin Guo, Jun Han, Yongning Zhang, Hanchun Yang
    2025, 24(6): 2443-2447.  DOI: 10.1016/j.jia.2024.11.020
    Abstract ( )   PDF in ScienceDirect