SiDWARF4 encodes an ent-copalyl diphosphate synthase (CPS) required for gibberellin biosynthesis and morphogenesis in foxtail millet

doi:10.1016/j.jia.2024.12.002

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SiDWARF4 encodes an ent-copalyl diphosphate synthase (CPS) required for gibberellin biosynthesis and morphogenesis in foxtail millet

Yongchao Li¹, Mengmeng Sun¹, Rui Zhao¹, Jiayi Chen¹, Yunhao Chen¹, Shuqi Dong^1,², Xiangyang Yuan^1,², Xiaorui Li^1,², Lulu Gao¹, Guanghui Yang¹, Peiyong Xin³, Shujing Cheng³, Jinfang Chu^3,⁴, Xiaoqian Chu¹^#, Jia-Gang Wang¹^#

¹Houji Laboratory in Shanxi Province, College of Agriculture, Shanxi Agricultural University, Taigu 030801, China

²State Key Laboratory of Sustainable Dryland Agriculture (in Preparation), Shanxi Agricultural University, Taigu 030801, China

³National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

⁴University of Chinese Academy of Sciences, Beijing 100049, China

Highlights

1. The phenotype of dwarf4 is due to the abnormal intersegmental elongation and reduced gibberellin synthesis.

2. The SiDWARF4 encodes the CPS enzyme involved in GA biosynthesis in foxtail millet.

Abstract
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摘要

赤霉素在植物生长发育中起着重要作用。作物矮化主要与赤霉素合成或信号转导途径的突变有关。然而，对作物中与赤霉素合成和调控有关的基因知之甚少。本研究分离到一株极端矮化的自然矮杆突变体dwarf4。基于BSA-seq，证实了由SiDWARF4编码的古巴焦磷酸合成酶（CPS）在谷子GA生物合成过程中催化了牻牛儿基牻牛儿基焦磷酸（GGPP）向古巴焦磷酸（CPP）的转化。在dwarf4中，CPS酶底物GGPP水平升高，导致GAs含量降低。外源GA₃处理可以恢复dwarf4的异常表型。SiDWARF4敲除系表型与dwarf4一致。与对照Ci846相比，GGPP含量升高，GAs含量降低，与dwarf4的结果一致。研究结果对改良谷子株型和提高谷子种植密度具有一定的指导意义。

Online: 04 December 2024

Fund:

This work was supported by National Natural Science Foundation (32200222 for J. W), the High-level Talents Start-up Fund of Shanxi Agricultural University, China (J242198006 for J. W), the Start-up Fund of Shanxi Agricultural University, China (2021BQ84 for X. C), the Shanxi Province Outstanding Doctoral and Post-Doctoral Scholarship Award Foundation, China (SXBYKY2021055 for J. W, SXBYKY2022033 for X. C) and the Houji Laboratory Foundation, China (202204010910001-32 for J. W).

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Yongchao Li, Mengmeng Sun, Rui Zhao, Jiayi Chen, Yunhao Chen, Shuqi Dong, Xiangyang Yuan, Xiaorui Li, Lulu Gao, Guanghui Yang, Peiyong Xin, Shujing Cheng, Jinfang Chu, Xiaoqian Chu, Jia-Gang Wang. 2024. SiDWARF4 encodes an ent-copalyl diphosphate synthase (CPS) required for gibberellin biosynthesis and morphogenesis in foxtail millet. Journal of Integrative Agriculture, Doi:10.1016/j.jia.2024.12.002

Bensen R J, Johal G S, Crane V C, Tossberg J T, Schnable P S, Meeley R B, Briggs S P. 1995. Cloning and characterization of the maize An1 gene. The Plant Cell, 7, 75-84.

Brosius J. 1991. Retroposons-seeds of evolution. Science, 251, 753-753.

Derkx A, Baumann U, Cheong J, Mrva K, Sharma N, Pallotta M, Mares D. 2021. A major locus on wheat chromosome 7B associated with late-maturity α-amylase encodes a putative ent-copalyl diphosphate synthase. Frontiers in Plant Science, 12, 637685.

Diao X. 2019. Progresses in stress tolerance and field cultivation studies of orphan cereals in China. Scientia Agricultura Sinica, 52, 3943-3949. (in Chinese)

Diao X, Wang L, Zhi H, Zhang J, Li S, Cheng R. 2024. Development, genetic deciphering and breeding utilization of dwarf lines in foxtail millet. Acta Agronomica Sinica, 50, 265-279. (in Chinese).

Fan X, Tang S, Zhi H, He M, Ma W, Jia Y, Zhao B, Jia G, Diao X. 2017. Identification and fine mapping of SiDWARF3 (D3), a pleiotropic locus controlling environment‐independent dwarfism in foxtail millet. Crop Science, 57, 2431-2442.

Peng J, Richards D E, Hartley N M, Murphy G P, Devos K M, Flintham J E, Beales J, Fish L J, Worland A J, Pelica F. 1999. ‘Green revolution’genes encode mutant gibberellin response modulators. Nature, 400, 256-261.

Sakamoto T, Miura K, Itoh H, Tatsumi T, Ueguchi-Tanaka M, Ishiyama K, Kobayashi M, Agrawal G K, Takeda S, Abe K. 2004. An overview of gibberellin metabolism enzyme genes and their related mutants in rice. Plant Physiology, 134, 1642-1653.

Shani E, Hedden P, Sun T P. 2024. Highlights in gibberellin research: A tale of the dwarf and the slender. Plant Physiology, 195, 111.

Sun T, Kamiya Y. 1994. The Arabidopsis GA1 locus encodes the cyclase ent-kaurene synthetase A of gibberellin biosynthesis. The Plant Cell, 6, 1509-1518.

Tian B, Wang J, Zhang L, Li Y, Wang S, Li H. 2010. Assessment of resistance to lodging of landrace and improved cultivars in foxtail millet. Euphytica, 172, 295-302.

Xue C, Zhi H, Fang X, Liu X, Tang S, Chai Y, Zhao B, Jia G, Diao X. 2016. Characterization and fine mapping of SiDWARF2 (D2) in foxtail millet. Crop Science, 56, 95-103.

Zhao M, Zhi H, Zhang X, Jia G, Diao X. 2019. Retrotransposon-mediated DELLA transcriptional reprograming underlies semi-dominant dwarfism in foxtail millet. The Crop Journal, 7, 458-468.

Zhou F, Wang C, Gutensohn M, Jiang L, Zhang P, Zhang D, Dudareva N, Lu S. 2017. A recruiting protein of geranylgeranyl diphosphate synthase controls metabolic flux toward chlorophyll biosynthesis in rice. Proceedings of the National Academy of Sciences of the United States of America, 114, 6866-6871.

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