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Special Issue:
线虫Nematology
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| Morphological and molecular characterizations of cereal cyst nematode Heterodera avenae Wollenweber, 1924 from the Czech Republic |
| Shesh Kumari |
| Division of Crop Protection and Plant Health, Crop Research Institute, Prague 16106, Czech Republic |
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Abstract The cereal cyst nematode, Heterodera avenae Wollenweber, 1924, is a major pest of cereal crops throughout the world and causes serious yield losses, especially of wheat. Previous studies have shown that this species is widely distributed in the Czech Republic. In this study, seven populations of H. avenae were molecularly studied, and one population was morphologically described. Three regions (18S, 28S, and internal transcribed spacer 1) of ribosomal DNA were sequenced and the analysis of the 18S gene of six populations did not reveal any variation, whereas the internal transcribed spacer 1 and 28S sequences of six populations differed by only two nucleotides from a population in ?ilina. Precise and quick identification of cereal cyst nematodes is important for effective control measures and ribosomal sequence analyses of seven populations in this study will be useful in future phylogenetic studies of Heterodera spp. occurring in the Czech Republic.
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Received: 07 July 2016
Accepted:
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| Fund: This work was supported by the National Agency of Agriculture Research of the Czech Republic (QJ1230159). |
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Corresponding Authors:
Shesh Kumari, Tel: +420-233022436, E-mail: kumari@vurv.cz
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Cite this article:
Shesh Kumari.
2017.
Morphological and molecular characterizations of cereal cyst nematode Heterodera avenae Wollenweber, 1924 from the Czech Republic. Journal of Integrative Agriculture, 16(03): 532-539.
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| Akar T, Caliskan M, Nicol J M, Uranbey S, Sahin E, Yazar S, William M, Braun H J. 2009. Molecular characterization of cereal cyst nematode diagnostic markers Cre1 and Cre3 in some winter wheat germplasm and their potential use against Heterodera filipjevi. Field Crops Research, 114, 320–323.Bekal S, Gauthier J P, Rivoal R. 1997. Genetic diversity among a complex of cereal cyst nematodes inferred from RFLP analysis of the ribosomal internal transcribed spacer region. Genome, 40, 479–486.Brown D J F, Boag B. 1988. An examination of methods used to extract virus-vector nematodes (Nematoda: Longidoridae and Trichodoridae) from soil samples. Nematologia Mediterranea, 16, 93–99.Cherry T, Szalanski A L, Todd T C, Powers T O. 1997. The internal transcribed spacer region of Belonolaimus (Nemata, Belonolaimidae). Journal of Nematology, 29, 23–29. Handoo Z A. 2002. A key and compendium to species of the Heterodera avenae group (Nematoda: Heteroderidae). Journal of Nematology, 34, 250–262.Holterman M, Rybarczyk K, Elsen S V D, Megen H V, Mooyman P, Santiago R P, Bongers T, Bakker J, Helder J. 2008. A ribosomal DNA-based framework for the detection and quantification of stress-sensitive nematode families in terrestrial habitats. Molecular Ecology Resources, 8, 23–34.Holterman M, Wurff A V D, Elsen S V D, Megen H V, Bongers T, Holovachov O, Bakker J, Helder J. 2006. Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades. Molecular Biology and Evolution, 23, 1792–1800. Ibrahim A A M, Al-Hazmi A S, Al-Yahya F A, Alderfasi A A. 1999. Damage potential and reproduction of Heterodera avenae on wheat and barley under Saudi field conditions. Nematology, 1, 625–630.Jones J T, Haegeman A, Danchin E G J, Gaur H S, Helder J, Jones M G K, Kikuchi T, Manzanilla-López R, Palomares-Rius J E, Wesemael W M L, Perry R N. 2013. Top 10 plant-parasitic nematodes in molecular plant pathology. Molecular Plant Pathology, 14, 946–961.Maafi Z T, Sturhan D, Kheiri A, Geraert E. 2007. Species of the Heterodera avenae group (Nematoda: Heteroderidae) from Iran. Russian Journal of Nematology, 15, 49–58.Maafi Z T, Subbotin S A, Moens M. 2003. Molecular identification of some cyst-forming nematodes (Heteroderidae) from Iran and a phylogeny based on ITSrDNA sequences. Nematology, 5, 99–111.Madani M, Vovlas N, Castillo P, Subbotin S A, Moens M. 2004. Molecular characterization of cyst nematode species (Heterodera spp.) from the Mediterranean basin using RFLPs and sequences of ITS-rDNA. Journal of Phytopathology, 152, 229–234.Mulvey R H. 1972. Identification of Heterodera cysts by terminal and cone top structures. Canadian Journal of Zoology, 50, 1277–1292.Mulvey R H, Golden A M. 1983. An illustrated key to the cyst-forming genera and species of Heteroderidae in the Western hemisphere with species morphometrics and distribution. Journal of Nematology, 15, 1–59.Nicol J M, Rivoal R. 2008. Global knowledge and its application for the integrated control and management of nematodes on wheat. In: Ciancio A, Mukerji K G, eds., Integrated Management and Biocontrol of Vegetable and Grain Crops Nematodes. Springer, Netherlands. pp. 243–287.Rivoal R, Cook R. 1993. Nematode pests of cereals. In: Evans K, Trudgill D L, Webster J M, eds., Plant Parasitic Nematodes in Temperate Agriculture Wallingford. CAB International, UK. pp. 259–303.Romeo M D. 1977. The morphology of Heterodera avenae in Spain. Nematologia Mediterranea, 5, 291–297.Sabová M, Lišková M, Valocká B, Vargová V. 1990. Pathotypes of Heterodera avenae Woll. in Czechoslovakia. Ochrana Rostlin, 26, 291–307. (in Slovak)Sabová M, Valocká B, Lišková M, Vargová V. 1988. The first finding of Heterodera latipons Franklin, 1969, on grass in stands in Czechoslovakia. Helminthologia, 25, 201–206.Sabová M, Valocká B, Lišková M, Vargová V. 1989a. The effect of Heterodera Avenae Woll., 1924 on cereals yield in the Czech Republic. Ochrana Rostlin, 25, 299–302. (in Slovak)Sabová M, Valocká B, Lišková M, Vargová V, Marek J. 1989b. Occurrence and distribution of Heterodera avenae Woll, 1924 in the Czech Republic. Ochrana Rostlin, 25, 59–70. (in Slovak)Shepherd A M. 1986. Extraction and estimation of cyst nematodes. In: Southey J E, ed., Laboratory Methods for Work With Plant and Soil Nematodes. HMSO Books, Norwich, UK. pp. 31–49.Stanton J M, Mcnicol C D, Steele V. 1998. Non-manual lysis of second-stage Meloidogyne juveniles for identification of pure and mixed samples based on the polymerase chain reaction. Australasian Plant Pathology, 27, 112–115. Subbotin S A, Mundo-Ocampo M, Baldwin J G. 2010. Systematics of cyst nematodes (Nematoda: Heteroderinae). In: Hunt D J, Perry R N, eds., Nematology Monograps and Perspectives. vol. 8B. Leiden, The Netherlands, Brill. p. 512.Subbotin S A, Rumpenhorst H J, Sturhan D. 1996. Morphological and electrophoretic studies on populations of the Heterodera avenae complex from the former USSR. Russian Journal of Nematology, 4, 29–39.Subbotin S A, Sturhan D, Rumpenhorst H J, Moens M. 2003. Molecular and morphological characterisation of the Heterodera avenae species complex (Tylenchida: Heteroderidae). Nematology, 5, 515–538.Subbotin S A, Waeyenberge L, Molokanova I A, Moens M. 1999. Identification of Heterodera avenae group species by morphometrics and rDNA-RFLPs. Nematology, 1, 195–207.Toumi F, Waeyenberge L, Viaene N, Dababat A, Nicol J M, Ogbonnaya F, Moens M. 2013. Development of two species-specific primer sets to detect the cereal cyst nematodes Heterodera avenae and Heterodera filipjevi. European Journal of Plant Pathology, 136, 613–624.Valocká B, Sabová M, Lišková M. 1993. Distribution of Heterodera avenae in Czecho-Slovakia. Nematologia Mediterranea, 21, 123–124.Vrain T C, Wakarchuk D A, Levesque A C, Hamilton R I. 1992. Intraspecific rDNA restriction fragment length polymorphism in the Xiphinema americanum group. Fundamental and Applied Nematology, 15, 563–573.Williams M R, Siddiqi M R. 1972. Heterodera avenae. C.I.H. Descriptions of Plantparasitic Nematodes. Set 1, No. 2. St. Albans, Herts, England.Yan G, Smiley R W. 2010. Distinguishing Heterodera filipjevi and H. avenae using polymerase chain reaction-restriction fragment length polymorphism and cyst morphology. Phytopathology, 100, 216–224.Yan G P, Smiley R W, Okubara P A, Skantar A M. 2013. Species-specific PCR assays for differentiating Heterodera filipjevi and H. avenae. Plant Disease, 97, 1611–1619. |
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