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Journal of Integrative Agriculture  2024, Vol. 23 Issue (8): 2571-2588    DOI: 10.1016/j.jia.2024.04.022
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Fine-mapping and primary analysis of candidate genes associated with seed coat color in mung bean (Vigna radiata L.)
Qian Wang1, Huimin Cao1, Jingcheng Wang1, Zirong Gu1, Qiuyun Lin2, Zeyan Zhang1, Xueying Zhao1, Wei Gao1, Huijun Zhu1, Hubin Yan1, Jianjun Yan1, Qingting Hao1, Yaowen Zhang1#
1 College of Agronomy, Shanxi Agricultural University, Taiyuan 030031, China
2 Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
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摘要  
绿豆种皮色与种子的外观品质及商品性密切相关。影响绿豆种皮色的物质主要为黄酮类化合物,具有重要的药用价值。定位绿豆种皮颜色基因将有助于开发新品种,提高其应用价值。本研究利用冀绿9号(黑色种皮)和BIS9805(绿色种皮)构建了一个由546株后代组成的F2定位群体。利用BSA-seq方法和KASP分子标记,将种皮颜色相关的候选区域定位在4号染色体上,最终缩小到0.66 Mb区间之内,包含8个候选基因。结合转录组和代谢组分析发现,8个候选基因中有3个基因(LOC106758748、LOC106758747和LOC106759075)差异表达,可能导致冀绿9号和BIS9805之间类黄酮代谢物含量差异。这些研究结果为绿豆种皮颜色相关基因的克隆和加快分子标记辅助选择育种提供了理论依据。


Abstract  
Pre-harvest sprouting (PHS) poses a significant global challenge to cereal production, impacting both yield and quality.  Seed coat color affects the appearance and commodity quality of mung beans (Vigna radiata L.).  The substances that affect mung bean seed coat color are mainly flavonoids, which have important medicinal value.  Mapping the seed coat color gene in mung beans would facilitate the development of new varieties and improve their value.  In this study, an F2 mapping population consisting of 546 plants was constructed using Jilv9 (black seed coat) and BIS9805 (green seed coat).  Using bulk segregated analysis (BSA) sequencing and kompetitive allele-specific PCR (KASP) markers, the candidate region related to seed coat color was finally narrowed to 0.66 Mb on chromosome (Chr.) 4 and included eight candidate genes.  Combined transcriptome and metabolome analyses showed that three of the eight candidate genes (LOC106758748, LOC106758747, and LOC106759075) were differentially expressed, which may have caused the differences in flavonoid metabolite content between Jilv9 and BIS9805.  These findings can provide a research basis for cloning the genes related to seed coat color and accelerate molecular marker-assisted selection breeding in mung beans.


Keywords:  mung bean        seed coat color        bulk segregated analysis sequencing        transcriptome        metabolism        flavonoids  
Received: 19 December 2023   Accepted: 11 March 2024
Fund: 
This research was partially supported by the National Natural Science Foundation of China (32301928), the Basic Research Program of Shanxi Province, China (20210302124504), the China Agriculture Research System of MOF and MARA-Food Legumes (CARS-08-G10), the National Laboratory Project of Coarse Grain Germplasm Resources Innovation and Molecular Breeding, China (K462202040-01), the Ph D of Shanxi Agricultural University Scientific Research Start-up Project, China (2021BQ43), and the Scientific Research Project of Shanxi Agricultural University, China (YZGC098).
About author:  Qian Wang, E-mail: 15101537964@163.com; #Correspondence Yaowen Zhang, E-mail: zyw8118571@126.com

Cite this article: 

Qian Wang, Huimin Cao, Jingcheng Wang, Zirong Gu, Qiuyun Lin, Zeyan Zhang, Xueying Zhao, Wei Gao, Huijun Zhu, Hubin Yan, Jianjun Yan, Qingting Hao, Yaowen Zhang. 2024. Fine-mapping and primary analysis of candidate genes associated with seed coat color in mung bean (Vigna radiata L.). Journal of Integrative Agriculture, 23(8): 2571-2588.

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