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Journal of Integrative Agriculture  2023, Vol. 22 Issue (4): 1159-1171    DOI: 10.1016/j.jia.2022.08.067
Animal Science · Veterinary Medicine Advanced Online Publication | Current Issue | Archive | Adv Search |
Use of transcriptome sequencing to explore the effect of CSRP3 on chicken myoblasts
SHAN Yan-ju1, JI Gai-ge1, ZHANG Ming1, LIU Yi-fan1, 2, TU Yun-jie1, JU Xiao-jun1, SHU Jing-ting1#, ZOU Jian-min1

1 Key Laboratory for Poultry Genetics and Breeding of Jiangsu Province, Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou 225125, P.R.China 

2 Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education, Yangzhou University, Yangzhou 225009, P.R.China

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The mechanisms that regulate the specificity and maintenance of chicken muscle fiber types remain largely unknown.  In mammals, CSRP3 has been shown to play a vital role in the maintenance of typical muscle structure and function.  This study investigated the role that CSRP3 plays in chicken skeletal muscle.  First, the antibody against chicken CSRP3 protein was prepared, and the expression levels of the mRNA and protein of the CSRP3 gene in four chicken skeletal muscles with different myofiber compositions were compared.  Then the effects of CSRP3 silencing on the expression profile of chicken myoblast transcriptomes were analyzed.  The results showed that the expression levels of the mRNA and protein of the CSRP3 gene were both associated with the composition of fiber types in chicken skeletal muscles.  A total of 650 genes with at least 1.5-fold differences (Q<0.05) were identified, of which 255 genes were upregulated and 395 genes were downregulated by CSRP3 silencing.  Functional enrichment showed that several pathways, including adrenergic signaling in cardiomyocytes, adipocytokine signaling pathway and apelin signaling pathway, were significantly (P<0.05) enriched both in differentially expressed genes and all expressed genes.  The co-expressed gene network suggested that CSRP3 silencing caused a compensatory upregulation (Q<0.05) of genes related to the assembly of myofibrils, muscle differentiation, and contraction.  Meanwhile, two fast myosin heavy chain genes (MyH1B and MyH1E) were upregulated (Q<0.05) upon CSRP3 silencing.  These results suggested that CSRP3 plays a crucial role in chicken myofiber composition, and affects the distribution of chicken myofiber types, probably by regulating the expression of MyH1B and MyH1E.

Keywords:  CSRP3        chicken       myofiber type       transcriptome sequencing  
Received: 15 October 2021   Accepted: 05 May 2022
Fund: This work was supported by the earmarked fund for China Agriculture Research System (CARS-41), the earmarked fund for Jiangsu Agricultural Industry Technology System, China (JATS[2021]396), the Special Fund for Major Breeding Programs in Jiangsu Province (PZCZ201728), the Natural Science Foundation of Jiangsu Province (BK20161322, BK20211121, and BK20210955), the Projects of Key Laboratory for Poultry Genetics and Breeding of Jiangsu Province (JQLAB-ZZ-201703), the Open Project Program of Joint International Research 
Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education, Yangzhou University,  China (JILAR-KF202020), the Yangzhou Science and Technology Support Program for Modem Agriculture (YZ2021029), the Jiangsu Provincal Agricultural Independent Innovation Fund Project (CX(21)2011-1),
and the Independent Scientific Foundation of Public Welfare Scientific Institutes of Jiangsu Province (BM2018026).
About author:  SHAN Yan-ju, Tel: +86-514-85599076, E-mail: shanyanjusyj; #Correspondence SHU Jing-ting, Tel: +86-514-85599076, E-mail:

Cite this article: 

SHAN Yan-ju, JI Gai-ge, ZHANG Ming, LIU Yi-fan, TU Yun-jie, JU Xiao-jun, SHU Jing-ting, ZOU Jian-min. 2023. Use of transcriptome sequencing to explore the effect of CSRP3 on chicken myoblasts. Journal of Integrative Agriculture, 22(4): 1159-1171.

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