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Journal of Integrative Agriculture  2023, Vol. 22 Issue (4): 1082-1092    DOI: 10.1016/j.jia.2022.08.121
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Characterization of the microbial community response to replant diseases in peach orchards

LI Wei-hua1, 2*, CHEN Peng1, 3*, WANG Yu-zhu4, LIU Qi-zhi1#

1 Laboratory of Entomology and Nematology, College of Plant Protection, China Agricultural University, Beijing 100193, P.R.China

2 State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R.China

3 Key Laboratory of Natural Enemies Insects of Ministry of Agriculture and Rural Affairs, Shandong Provincial Engineering Technology Research Center on Biocontrol of Crop Diseases and Insect Pest, Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, P.R.China

4 Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100093, P.R.China

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以不同年份再植桃园根系土壤为材料,探究再植桃园微生物群落结构的变化情况,并进一步揭示不同年份的再植桃园微生物群落和土壤养分之间的关系,以期为桃树再植病调控提供理论依据。分别收集非再植(NRS)和再植(RS(再植1RS1、再植3RS3、再植5RS5、再植7RS7、再植9RS9、再植11RS11)桃园桃树的根际土壤,利用高通量测序技术测定土壤细菌和真菌群落的多样性,同时采用RDA分析土壤微生物群落与土壤环境因子之间的关系。结果显示,RS早期(1-5年)的土壤养分含量低于NRS,但随着桃树种植年限的增加,它们之间的差异逐渐缩小,直至达到相近的水平。细菌和真菌群落的alpha多样性指数表明,RSNRS含有更高丰度的细菌和真菌OUT含量NMDSANOSIM分析表明,土壤细菌和真菌群落显著受种植年限影响(p<0.01),变化主要发生在种植1年和9年。从目的分类水平看,再植桃园土壤中,Sphingobacteriales, Burkholderiales 和 Actinomycetales显著发生变化。一些与生物修复相关的细菌,如Burkholderiales目 和 Intrasporangiaceae纲,以及一些有害的病原真菌,如Penicillium属 和 Ophiostomatales纲,在再植桃园中显着增加(LDA> 3.0)。此外, RDA结果表明微生物群落的组成与环境各因子(pHAPAN AK间存在密切相关。从细菌门的分类水平看,这些环境变量与Acidobacteria, Chloroflexi, 和 Actinobacteria呈正相关,与Proteobacteria 和 Firmicutes呈负相关。在真菌门水平中,Basidiomycota门在 pHAP AN 增加的环境中增强,而Ascomycota, Chytridiomycota 和 Zygomycota门与 AK 呈正相关。RS的细菌和真菌群落多样性高于NRS树再植病害的发生与土壤微生物群落的变化密切相关。我们的研究结果详细阐明了不同年份的 NRS RS微生物群落的变化情况以及两者之间土壤理化和微生物群落变化之间的关系。这些结果使人们更加深入的了解再植桃园微生物群落的变化,为桃树再植病的解决提供思路。


This study attempted to monitor the development of microbial communities and reveal the correlation between the soil microbial community and soil nutrient factors over different years following the replanting of peach trees.  The replanted soil (RS) and nonreplanted soil (NRS) were collected from peach orchards with different growth years (1, 3, 5, 7, 9, 11, and 13 years) in the same region.  The soil bacterial and fungal community diversities were analyzed by high-throughput sequencing technology.  Redundancy analysis (RDA) was used to show the correlation between the soil microbial community and environmental variables.  The alpha diversities of the bacterial and fungal communities indicated that RS contained a higher abundance of bacterial and fungal operational taxonomic units (OTUs) than NRS.  NMDS and ANOSIM analyses showed that the soil bacterial and fungal communities were significantly (P<0.01) affected by planting years, and that the main changes occurred in the first and ninth planting years.  The presence of the bacterial orders Sphingobacteriales, Burkholderiales and Actinomycetales changed significantly after replanting.  Some bacteria associated with bioremediation, such as Burkholderiales and Intrasporangiaceae, and some harmful pathogens, such as Penicillium and Ophiostomatales, significantly increased after replanting (LDA score>3.0).  In addition, the soil nutrient contents were lower in RS than in NRS in the early stage (1–5 years), and the RDA showed that bacterial and fungal phyla are closely associated with environmental variables, including the potential of hydrogen (pH), ammonium nitrogen (AN), available phosphorus (AP) and available potassium (AK).  These results lead to a deeper understanding of the microbial responses to replanting in peach orchards. 

Keywords:  replant disease       complex syndrome       microbial community       high-throughput sequencing       environmental variables  
Received: 28 January 2022   Accepted: 06 May 2022
Fund: This work was supported by the National Science and Technology Support Program of China (2014BAD20B01 and 2014BAD16B07), the National Key R&D Program of China (2019YFE0120400), and the Agricultural Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences, China (CXGC2021B13 and CXGC2022D06).
About author:  Received 28 January, 2022 Accepted 6 May, 2022 LI Wei-hua, E-mail:; CHEN Peng, E-mail:; #Correspondence LIU Qi-zhi, Mobile: +86-18519264197, E-mail: * These authors contributed equally to this study.

Cite this article: 

LI Wei-hua, CHEN Peng, WANG Yu-zhu, LIU Qi-zhi. 2023. Characterization of the microbial community response to replant diseases in peach orchards. Journal of Integrative Agriculture, 22(4): 1082-1092.

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