Zea mays L. , early developing tassel , transcriptome , drought tolerance," /> Zea mays L. , early developing tassel , transcriptome , drought tolerance,"/> Zea mays L. , early developing tassel , transcriptome , drought tolerance,"/>
Please wait a minute...
Journal of Integrative Agriculture  2018, Vol. 17 Issue (06): 1276-1288    DOI: 10.1016/S2095-3119(17)61777-5
Crop Science Advanced Online Publication | Current Issue | Archive | Adv Search |
Transcriptomes of early developing tassels under drought stress reveal differential expression of genes related to drought tolerance in maize
WANG Nan1*, LI Liang1, 2*, GAO Wen-wei2*, WU Yong-bo1, YONG Hong-jun1, WENG Jian-feng1, LI Ming-shun1, ZHANG De-gui1, HAO Zhuan-fang1, LI Xin-hai1 
1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2 College of Agriculture, Xinjiang Agricultural University, Urumqi 830000, P.R.China
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
Tassel, the male reproductive organs in maize, its development is adversely affected by drought during tasseling.  To determine drought tolerance mechanisms of tassel differentiation at transcriptome level, RNA-Seq was performed using  RNA of early developing tassel from 10 maize inbred lines under well-watered (control) and drought-stressed conditions, respectively.  Results showed that the most active pathway for drought stress in maize were related to metabolic regulation at RNA level.  And some genes, encoding enzymes involved in carbohydrate and lipid metabolism, were significantly down-regulated in drought-stressed plants.  While, the transcription factors and genes, encoding catabolic or degradative enzymes, were over-expressed in maize early developing tassels under drought-stressed conditions, and among them, the transcripts of genes encoding exon-junction complexes involved in ‘RNA transcript’ and ‘mRNA surveillance’ pathways were significantly affected by drought stress.  In addition, many other genes related to drought stress showed transcriptional changes at the later period of stress.
Keywords:  Zea mays L. ')" href="#">  
Received: 24 May 2017   Accepted:
Fund: This research was jointly funded by the National Natural Science Foundation of China (31661143010) and the Pilot Project of Breeding of the Seven Major Crops, China (2016YFD0101803).
Corresponding Authors:  Correspondence HAO Zhuan-fang, Tel: +86-10-82108596, Fax: +86-10-82108747, E-mail: haozhuanfang@163.com; LI Xin-hai, E-mail: lixinhai@caas.cn    
About author:  * These authors contributed equally to this study.
E-mail this article Zea mays L. | early developing tassel | transcriptome | drought tolerance”. Please open it by linking:https://www.chinaagrisci.com/Jwk_zgnykxen/EN/abstract/abstract11710.shtml" name="neirong"> Zea mays L. | early developing tassel | transcriptome | drought tolerance">
Add to citation manager
E-mail Alert
Articles by authors
LI Liang
GAO Wen-wei
WU Yong-bo
YONG Hong-jun
WENG Jian-feng
LI Ming-shun
ZHANG De-gui
HAO Zhuan-fang
LI Xin-hai

Cite this article: 

WANG Nan, LI Liang, GAO Wen-wei, WU Yong-bo, YONG Hong-jun, WENG Jian-feng, LI Ming-shun, ZHANG De-gui, HAO Zhuan-fang, LI Xin-hai. 2018. Transcriptomes of early developing tassels under drought stress reveal differential expression of genes related to drought tolerance in maize. Journal of Integrative Agriculture, 17(06): 1276-1288.

Aarts M G M, Hodge R, Kalantidis K, Florack D, Wilson Z A, Mulligan B J, Stiekema W J, Scott R, Pereira A. 1997. The Arabidopsis MALE STERILITY 2 protein shares similarity with reductases in elongation/condensation complexes. The Plant Journal, 12, 615–623.
Aarts M G M, Keijzer C J, Stiekema W J, Pereira A. 1995. Molecular characterization of the CER1 gene of Arabidopsis involved in epicuticular wax biosynthesis and pollen fertility. The Plant Cell, 7, 2115–2127.
Abdi H. 2007. The bonferonni and Šidák corrections for multiple comparisons. [2017-04-12]. http://www.utdallas.edu/~herve/Abdi-Bonferroni2007-pretty.pdf
Andersen M N, Asch F, Wu Y, Jensen C R, Naested H, Mogensen V O, Koch K E. 2002. Soluble invertase expression is an early target of drought stress during the critical, abortion-sensitive phase of young ovary development in maize. Plant Physiology, 130, 591–604.
Bate N J, Niu X P, Wang Y W, Reimann K S, Helentjaris T G. 2004. An invertase inhibitor from maize localizes to the embryo surrounding region during early kernel development. Plant Physiology, 134, 246–254.
Benjamini Y, Yekutieli D. 2001. The control of the false discovery rate in multiple testing under dependency. The Annals of Statistics, 29, 1165–1188.
Chen Y Z, Cao J. 2015. Comparative analysis of dof transcription factor family in maize. Plant Molecular Biology Reporter, 33, 1245–1258.
Dong Y P, Fan G Q, Zhao Z L, Deng M J. 2014. Transcriptome expression profiling in response to drought stress in Paulownia Australis. International Journal of Molecular Sciences, 15, 4583–4607.
Etxebeste O, Herrero-Garcia E, Cortese MS, Garzia A, Oiartzabal-Arano E, de los Rios V, Ugalde U, Espeso E A. 2012. GmcA is a putative glucose-methanol-choline oxidoreductase required for the induction of asexual development in Aspergillus nidulans. PLoS ONE, 7, e40292.
Fu J J, Cheng Y B, Linghu J J, Yang X H, Kang L, Zhang Z X, Zhang J, He C, Du X M, Peng Z Y, Wang B, Zhai L H, Dai C M, Xu J B, Wang W D, Li X R, Zheng J, Chen L, Luo L H, Liu J J, et al. 2013. RNA sequencing reveals the complex regulatory network in the maize kernel. Nature Communications, 4, 2832.
Gao J, Zhang Y, Zhang C L, Qi F Y, Li X P, Mu S H, Peng Z H. 2014. Characterization of the floral transcriptome of Moso Bamboo (Phyllostachys edulis) at different flowering developmental stages by transcriptome sequencing and RNA-Seq analysis. PLoS ONE, 9, e98910.
Hao Z F, Li X H, Liu X L, Xie C X, Li M S, Zhang D G, Zhang S H. 2010. Meta-analysis of constitutive and adaptive QTL for drought tolerance in maize. Euphytica, 174, 165–177.
Hao Z F, Li X H, Su Z J, Xie C X, Li M S, Liang X L, Weng J F, Zhang D G, Li L, Zhang S H. 2011. A proposed selection criterion for drought resistance across multiple environments in maize. Breeding Science, 61, 101–108.
Kakumanu A, Ambavaram M M R, Klumas C, Krishnan A, Batlang U, Myers E, Grene R, Pereira A. 2012. Effects of drought on gene expression in maize reproductive and leaf meristem tissue revealed by RNA-Seq. Plant Physiology, 160, 846–867.
Kanehisa M, Araki M, Goto S, Hattori M, Hirakawa M, Itoh M, Katayama T, Kawashima S, Okuda S, Tokimatsu T, Yamanishi Y. 2008. KEGG for linking genomes to life and the environment. Nucleic Acids Research, 36, 480–484.
Li E, Wang S, Liu Y, Chen J G, Douglas C J. 2011. OVATE FAMILY PROTEIN4 (OFP4) interaction with KNAT7 regulates secondary cell wall formation in Arabidopsis thaliana. The Plant Journal, 67, 328–341.
Li R Q, Yu C, Li Y R, Lam T W, Yiu S M, Kristiansen K, Wang J. 2009. SOAP2: An improved ultrafast tool for short read alignment. Bioinformatics, 25, 1966–1967.
Liu C L, Hao Z F, Zhang D G, Xie C X, Li M S, Zhang X C, Yong H J, Zhang S H, Weng J F, Li X H. 2015. Genetic properties of 240 maize inbred lines and identity-by-descent segments revealed by high-density SNP markers. Molecular Breeding, 35, 1–12.
Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(T) (-Delta Delta C) method. Methods, 25, 402–408.
Lokesh, kiranmai, Pandurangaiah M, Babu S, Kumar N, Sudhakar C. 2013. Role of plant fatty acid elongase (3 keto acyl-CoA Synthase) gene in cuticular wax biosynthesis. Journal of Agriculture and Allied Sciences, 2, 35–42.
Matsuno M, Compagnon V, Schoch G A, Schmitt M, Debayle D, Bassard J E, Pollet B, Hehn A, Heintz D, Ullmann P, Lapierre C, Bernier F, Ehlting J, Werck-Reichhart D. 2009. Evolution of a novel phenolic pathway for pollen development. Science, 325, 1688–1692.
Mortazavi A, Williams B A, Mccue K, Schaeffer L, Wold B. 2008. Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nature Methods, 5, 621–628.
Nakashima K, Yamaguchi-Shinozaki K, Shinozaki K. 2014. The transcriptional regulatory network in the drought response and its crosstalk in abiotic stress responses including drought, cold, and heat. Frontiers in Plant Science, 5, 621–627.
Nariya H, Inouye S. 2002. Activation of 6-phosphofructokinase via phosphorylation by Pkn4, a protein Ser/Thr kinase of Myxococcus xanthus. Molecular Microbiology, 46, 1353–1366.
Okoniewski M J, Miller C J. 2006. Hybridization interactions between probesets in short oligo microarrays lead to spurious correlations. BMC Bioinformatics, 7, 276–279.
Osakabe Y, Yamaguchi-Shinozaki K, Shinozaki K, Tran L S. 2014. ABA control of plant macroelement membrane transport systems in response to water deficit and high salinity. The New Phytologist, 202, 35–49.
Perez-Diaz J, Wu T M, Perez-Diaz R, Ruiz-Lara S, Hong C Y, Casaretto J A. 2014. Organ- and stress-specific expression of the ASR genes in rice. Plant Cell Reports, 33, 61–73.
Saini H S. 1997. Effects of water stress on male gametophyte development in plants. Sex Plant Reprot, 10, 67–73.
Shen C X, Li D, He R H, Fang Z, Xia Y M, Gao J, Shen H, Cao M L. 2014. Comparative transcriptome analysis of RNA-seq data for cold-tolerant and cold-sensitive rice genotypes under cold stress. Journal of Plant Biology, 57, 337–348.
Shinozaki K, Yamaguchi-Shinozaki K, Seki M. 2003. Regulatory network of gene expression in the drought and cold stress responses. Current Opinion in Plant Biology, 6, 410–417.
Tange T, Nott A, Moore M J. 2004. The ever-increasing complexities of the exon junction complex. Current Opinion in Cell Biology, 16, 279–284.
Todaka D, Shinozaki K, Yamaguchi-Shinozaki K. 2015. Recent advances in the dissection of drought-stress regulatory networks and strategies for development of drought-tolerant transgenic rice plants. Frontiers in Plant Science, 6, 84.
Wang W J, Liu X W, Gai X S, Ren J J, Liu X F, Cai Y L, Wang Q, Ren H Z. 2015. Cucumis sativus L. WAX2 plays a pivotal role in wax biosynthesis, influencing pollen fertility and plant biotic and abiotic stress responses. Plant and Cell Physiology, 56, 1339–1354.
Wang Z, Gerstein M, Snyder M. 2009. RNA-seq: A revolutionary tool for transcriptomics. Nature Reviews Genetics, 10, 57–63.
Xiong L, Schumaker K S, Zhu J K. 2002. Cell signaling during cold, drought, and salt stress. The Plant Cell, 14, 165–183.
Xu H, Gao Y, Wang J B. 2012. Transcriptomic analysis of rice (Oryza sativa) developing embryos using the RNA-Seq technique. PLoS ONE, 7, e30646.
Yin F Q, Qin C, Gao J, Liu M, Luo X R, Zhang W Y, Liu H J, Liao X H, Shen Y U, Mao L K, Zhang Z M, Lin H J, Lubberstedt T, Pan G T. 2015. Genome-wide identification and analysis of drought-responsive genes and microRNAs in tobacco. Inter national Journal of Molecular Sciences, 16, 5714–5740.
Zhuang Y, Ren G J, Yue G D, Li Z X, Qu X, Hou G H, Zhu Y, Zhang J R. 2007. Effects of water-deficit stress on the transcriptomes of developing immature ear and tassel in maize. Plant Cell Reports, 26, 2137–2147.
[1] LÜ Jing, Satyabrata NANDA, CHEN Shi-min, MEI Yang, HE Kang, QIU Bao-li, ZHANG You-jun, LI Fei, PAN Hui-peng.

A survey on the off-target effects of insecticidal double-stranded RNA targeting the Hvβ´COPI gene in the crop pest Henosepilachna vigintioctopunctata through RNA-seq [J]. >Journal of Integrative Agriculture, 2022, 21(9): 2665-2674.

[2] WANG Bo, HUANG Tian-yu, YAO Yuan, Frederic FRANCIS, YAN Chun-cai, WANG Gui-rong, WANG Bing. A conserved odorant receptor identified from antennal transcriptome of Megoura crassicauda that specifically responds to cis-jasmone[J]. >Journal of Integrative Agriculture, 2022, 21(7): 2042-2054.
[3] ZHOU Cheng-zhe, ZHU Chen, LI Xiao-zhen, CHEN Lan, XIE Si-yi, CHEN Guang-wu, ZHANG Huan, LAI Zhong-xiong, LIN Yu-ling, GUO Yu-qiong. Transcriptome and phytochemical analyses reveal roles of characteristic metabolites in the taste formation of white tea during withering process[J]. >Journal of Integrative Agriculture, 2022, 21(3): 862-877.
[4] SHI Hai-yan, CAO Li-wen, XU Yue, YANG Xiong, LIU Shui-lin, LIANG Zhong-shuo, LI Guo-ce, YANG Yu-peng, ZHANG Yu-xing, CHEN Liang. Transcriptional profiles underlying the effects of salicylic acid on fruit ripening and senescence in pear (Pyrus pyrifolia Nakai)[J]. >Journal of Integrative Agriculture, 2021, 20(9): 2424-2437.
[5] WANG Chao-nan, LUAN Fei-shi, LIU Hong-yu, Angela R. DAVIS, ZHANG Qi-an, DAI Zu-yun, LIU Shi. Mapping and predicting a candidate gene for flesh color in watermelon[J]. >Journal of Integrative Agriculture, 2021, 20(8): 2100-2111.
[6] WU Tong, FENG Shu-yan, YANG Qi-hang, Preetida J BHETARIYA, GONG Ke, CUI Chun-lin, SONG Jie, PING Xiao-rui, PEI Qiao-ying, YU Tong, SONG Xiao-ming. Integration of the metabolome and transcriptome reveals the metabolites and genes related to nutritional and medicinal value in Coriandrum sativum[J]. >Journal of Integrative Agriculture, 2021, 20(7): 1807-1818.
[7] ZHAO Juan, LIU Ting, LIU Wei-cheng, ZHANG Dian-peng, DONG Dan, WU Hui-ling, ZHANG Tao-tao, LIU De-wen. Transcriptomic insights into growth promotion effect of Trichoderma afroharzianum TM2-4 microbial agent on tomato plants[J]. >Journal of Integrative Agriculture, 2021, 20(5): 1266-1276.
[8] CHENG Jin-tao, CHEN Hai-wen, DING Xiao-chen, SHEN Tai, PENG Zhao-wen, KONG Qiu-sheng, HUANG Yuan, BIE Zhi-long. Transcriptome analysis of the influence of CPPU application for fruit setting on melon volatile content[J]. >Journal of Integrative Agriculture, 2021, 20(12): 3199-3208.
[9] FU Fang-fang, PENG Ying-shu, WANG Gui-bin, Yousry A. EL-KASSABY, CAO Fu-liang. Integrative analysis of the metabolome and transcriptome reveals seed germination mechanism in Punica granatum L.[J]. >Journal of Integrative Agriculture, 2021, 20(1): 132-146.
[10] LAN Hao, ZHANG Zhan-feng, WU Jun, CAO He-he, LIU Tong-xian. Performance and transcriptomic response of the English grain aphid, Sitobion avenae, feeding on resistant and susceptible wheat cultivars[J]. >Journal of Integrative Agriculture, 2021, 20(1): 178-190.
[11] LING Ying-hui, ZHENG Qi, JING Jing, SUI Meng-hua, ZHU Lu, LI Yun-sheng, ZHANG Yun-hai, LIU Ya, FANG Fu-gui, ZHANG Xiao-rong . Switches in transcriptome functions during seven skeletal muscle development stages from fetus to kid in Capra hircus[J]. >Journal of Integrative Agriculture, 2021, 20(1): 212-226.
[12] ZHANG Da-wei, LIU Li-li, ZHOU Ding-gang, LIU Xian-jun, LIU Zhong-song, YAN Ming-li.
Genome-wide identification and expression analysis of anthocyanin biosynthetic genes in Brassica juncea
[J]. >Journal of Integrative Agriculture, 2020, 19(5): 1250-1260.
[13] HAO Lu-yang, LIU Xu-yang, ZHANG Xiao-jing, SUN Bao-cheng, LIU Cheng, ZHANG Deng-feng, TANG Huai-jun, LI Chun-hui, LI Yong-xiang, SHI Yun-su, XIE Xiao-qing, SONG Yan-chun, WANG Tian-yu, LI Yu .
Genome-wide identification and comparative analysis of drought related genes in roots of two maize inbred lines with contrasting drought tolerance by RNA sequencing
[J]. >Journal of Integrative Agriculture, 2020, 19(2): 449-464.
[14] Aejaz Ahmad DAR, Susheel SHARMA, Reetika MAHAJAN, Muntazir MUSHTAQ, Ankila SALATHIA, Shahid AHAMAD, Jag Paul SHARMA. Overview of purple blotch disease and understanding its management through chemical, biological and genetic approaches[J]. >Journal of Integrative Agriculture, 2020, 19(12): 3013-3024.
[15] WANG Jie, WEI Shao-bo, WANG Chun-chao, Najeeb Ullah KHAN, ZHANG Zhan-ying, WANG Wen-sheng, ZHAO Xiu-qin, ZHANG Hong-liang, LI Zi-chao, GAO Yong-ming. Transcriptome and metabolome profiling of unheading in F1 hybrid rice[J]. >Journal of Integrative Agriculture, 2020, 19(10): 2367-2382.
No Suggested Reading articles found!