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Journal of Integrative Agriculture  2018, Vol. 17 Issue (06): 1276-1288    DOI: 10.1016/S2095-3119(17)61777-5
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Transcriptomes of early developing tassels under drought stress reveal differential expression of genes related to drought tolerance in maize
WANG Nan1*, LI Liang1, 2*, GAO Wen-wei2*, WU Yong-bo1, YONG Hong-jun1, WENG Jian-feng1, LI Ming-shun1, ZHANG De-gui1, HAO Zhuan-fang1, LI Xin-hai1 
1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2 College of Agriculture, Xinjiang Agricultural University, Urumqi 830000, P.R.China
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Abstract  
Tassel, the male reproductive organs in maize, its development is adversely affected by drought during tasseling.  To determine drought tolerance mechanisms of tassel differentiation at transcriptome level, RNA-Seq was performed using  RNA of early developing tassel from 10 maize inbred lines under well-watered (control) and drought-stressed conditions, respectively.  Results showed that the most active pathway for drought stress in maize were related to metabolic regulation at RNA level.  And some genes, encoding enzymes involved in carbohydrate and lipid metabolism, were significantly down-regulated in drought-stressed plants.  While, the transcription factors and genes, encoding catabolic or degradative enzymes, were over-expressed in maize early developing tassels under drought-stressed conditions, and among them, the transcripts of genes encoding exon-junction complexes involved in ‘RNA transcript’ and ‘mRNA surveillance’ pathways were significantly affected by drought stress.  In addition, many other genes related to drought stress showed transcriptional changes at the later period of stress.
Keywords:  Zea mays L. ')" href="#">  
Received: 24 May 2017   Accepted: 05 June 2018
Fund: This research was jointly funded by the National Natural Science Foundation of China (31661143010) and the Pilot Project of Breeding of the Seven Major Crops, China (2016YFD0101803).
Corresponding Authors:  Correspondence HAO Zhuan-fang, Tel: +86-10-82108596, Fax: +86-10-82108747, E-mail: haozhuanfang@163.com; LI Xin-hai, E-mail: lixinhai@caas.cn    
About author:  * These authors contributed equally to this study.
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WANG Nan
LI Liang
GAO Wen-wei
WU Yong-bo
YONG Hong-jun
WENG Jian-feng
LI Ming-shun
ZHANG De-gui
HAO Zhuan-fang
LI Xin-hai

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WANG Nan, LI Liang, GAO Wen-wei, WU Yong-bo, YONG Hong-jun, WENG Jian-feng, LI Ming-shun, ZHANG De-gui, HAO Zhuan-fang, LI Xin-hai. 2018. Transcriptomes of early developing tassels under drought stress reveal differential expression of genes related to drought tolerance in maize. Journal of Integrative Agriculture, 17(06): 1276-1288.

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