Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (4): 728-736.doi: 10.3864/j.issn.0578-1752.2013.04.007

• PLANT PROTECTION • Previous Articles     Next Articles

Characteristics of Mating Types, Physiological Races, Metalaxyl Sensitivity and mtDNA Haplotypes of Phytophthora infestans in Sichuan Province

 LI  Hong-Hao, PENG  Hua-Xian, XI  Ya-Dong, WANG  Xiao-Li, LIU  Bo-Wei   

  1. Key Laboratory of Integrated Pest Management on Crops in Southwest, Ministry of Agriculture, Institute of Plant Protection, Sichuan Academy of Agricultural Science, Chengdu 610066
  • Received:2012-09-04 Online:2013-02-15 Published:2012-10-08

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Abstract: 【Objective】The objective of this study is to provide a scientific basis to control potato late blight through the systematical analysis of populations of Phytophthora infestans. 【Method】The mating types, metalaxyl sensitivity, physiological races and mtDNA haplotypes of P. infestans isolates collected in Sichuan during 2008 to 2011 were analyzed. 【Result】Most P. infestans isolates in Sichuan were A2 mating type with the frequency of 62.5%. The frequency of A1 mating type and self-fertility in the isolates were 18.8% and 18.4%, respectively. The isolates of A1 mating type were only detected in Jiulong and Puge Counties. Fifty-five races were identified among 192 isolates and race 1.2.3.4.5.6.7.8.9.10.11 were the dominant races in Sichuan. There were 99.48% isolates contained at least two virulence genes. The sensitivity test to metalaxyl showed that resistant, intermediate and sensitive isolates were detected in Sichuan with the frequency of 63.0%, 22.0% and 15.0%, respectively. The sensitive isolates distributed in Puge, Daofu and Jiulong Counties. Two mtDNA haplotypes,Ⅰa and Ⅱa, were detected from populations of P. infestans in Sichuan with the frequency of 97.4% and 2.6%, respectively, which belonged to the “new” populations, following the second global migration of the pathogen.Ⅰa haplotype distributed in all potato producing areas extensively, including A1 mating type, A2 mating type, self-fertility and unknown mating type isolates. 【Conclusion】The composition of populations of P. infestans is getting more complex in Sichuan. To control potato late blight scientifically it is necessary to explore new resistant germplasms, breed horizontal resistant varieties, dispose resistant varieties reasonably and take full advantage of the potato late blight forecasting system.

Key words: potato late blight, mating type, physiological race, metalaxyl sensitivity, mtDNA haplotype, Phytophthora infestans

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