中国野生刺葡萄抗白腐病NBS-LRR类抗病基因 同源序列的分离与鉴定

doi:10.3864/j.issn.0578-1752.2013.04.012

Abstract

Abstract: 【Objective】 The purpose of this study was to get white rot disease resistant homologous clips from Vitis davidii ‘Vitis davidii cv. Gaoshan 2’, which will provide an effective means for screening white rot disease resistant gene. 【Method】 According to the known plant resistance genes NBS-LRR conservative area, degenerate primers were designed and used to clone targeted resistance gene analogs using templates of genomic DNA and cDNA from white rot disease resistant variety of Vitis davidii ‘Vitis davidii cv. Gaoshan 2’. Then the gene expression analysis was performed. 【Result】 Ten NBS-LRR kinds of resistance gene analogs from Vitis davidii ‘Vitis davidii cv. Gaoshan 2’ were obtained, and their amino acid sequences showed rich polymorphism. Further analysis found that among the ten NBS sequences, four genes belong to non-TIR-NBS-LRR kind of resistance genes, six belong to TIR-NBS-LRR kind of resistance genes. Quantitative PCR analysis showed that NB7 gene was induced by white rot fungus, and NB7 gene was less abundantly expressed in the leaves of Vitis davidii, NBS6 gene is down-regulated expression after induced by white rot disease. 【Conclusion】 Ten RGAs were successfully obtained from Vitis davidii, and fluorescence quantitative PCR analysis showed that, both NB7 gene and NBS6 gene expression are induced by white rot fungus. The results of this study have layed a foundation for final cloning white rot disease resistant gene from Vitis.

Key words: Chinese wild grape , Vitis davidii , white rot disease , NBS-LRR , resistance gene homology sequence

ZHANG Ying, LI Feng, LIU Chong-Huai, FAN Xiu-Cai, SUN Hai-Sheng, JIANG Jian-Fu, ZHANG Guo-Hai. Isolation and Identification of NBS-LRR Resistance Gene Analogs Sequences from Vitis davidii[J].Scientia Agricultura Sinica, 2013, 46(4): 780-789.

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