Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (4): 678-685.doi: 10.3864/j.issn.0578-1752.2013.04.003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Functional Analysis of T1N6_22 in Arabidopsis thaliana Against the Infection of Pst DC3000

 HAO  Cong-Cong, ZHENG  Hui-Xin, JIA  Jiao, SI  He-Long, CHEN  Zhan, ZHAO  Bin, ZHANG  Jing, XING  Ji-Hong, DONG  Jin-Gao   

  1. 1.College of Life Science, Agricultural University of Hebei/Mycotoxin and Molecular Plant Pathology Laboratory, Baoding 071001, Hebei
    2.Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, Jilin; 3.Institute of Fruit Tree, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050061
  • Received:2012-11-05 Online:2013-02-15 Published:2013-01-14

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Abstract: 【Objective】 The objective of this study is to investigate the function of the T1N6_22, a resistant gene of Arabidopsis against B. cinerea, in Arabidopsis resistance to Pseudomonas syringae pv. tomato DC3000, to further analyze the mechanism of T1N6_22 genes in Arabidopsis resistance to Pst DC3000. 【Method】 The symptoms, increase of the content of callose and bacterial concentration of the t1n6_22 and t1n6_22/T1N6_22 in plants inoculated with Pst DC3000 were investigated to study the function of the T1N6_22 in Arabidopsis resistance to Pst. DC3000. RT-PCR technology was used to analyze the expression of T1N6_22 in Col-0 treatment with SA, JA, ET and the expression of defence-related genes in Col-0, the t1n6_22 and t1n6_22/T1N6_22 plants. Quantitative real-time PCR technology was used to analyze the expression of T1N6_22 and the key genes involved in the SA, JA/ET signal pathway in Col-0 inoculated with Pst DC3000. 【Result】 The t1n6_22 exhibited enhanced resistance to Pst DC3000, the Col-0 and complemental plants showed an obvious susceptibility to Pst DC3000. The expression of T1N6_22 in Col-0 treatment with SA was significantly enhanced, suggesting the expression of T1N6_22 induced by SA. Compared with the Col-0 and t1n6_22/T1N6_22 plants, the expression of PAL, PR4, PPO, SOD and CAT genes were downregulated in the t1n6_22 plants. The expression of T1N6_22 and the key genes involved in the SA, JA/ET signal pathway, such as PR1, PR3, PR5 and PDF1.2 genes, were upregulated in Col-0 after inoculation of Pst DC3000.【Conclusion】T1N6_22 gene is a negative regulatory component of Arabidopsis against Pst DC3000 and the T1N6_22 gene expression is induced by SA. T1N6_22 may be involved in the regulation of plant secondary metabolites in impact of the resistance to Pst DC3000 in Arabidopsis.

Key words: T1N6_22 , Arabidopsis thaliana , Pst DC3000 , disease-resistant pathway

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