Scientia Agricultura Sinica ›› 2007, Vol. 40 ›› Issue (2): 277-282 .

• PLANT PROTECTION • Previous Articles     Next Articles

cDNA-AFLP analysis of gene expression response to Xanthomonas oryzae pv. oryzae and identification of genes expressed differentially during this compatible

  

  1. 中国农业科学院植物保护研究所／植物病虫害生物学国家重点实验室
  • Received:2006-06-15 Revised:1900-01-01 Online:2007-02-10 Published:2007-02-10

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Abstract: To elucidate the nature of rice susceptible response to infection by Xanthomonas oryzae pv. oryzae (Xoo) at the genomic levels, analysis of the cDNA amplified fragment length polymorphism (AFLP) pattern of mock- and Xoo-inoculated rice suspension cultured cells was performed. Three hundred and sixty-three (9.1%) from approximately 4,000 cDNA fragments analyzed were differentially regulated (295 up-regulated and 68 down-regulated) 0.5, 3, 24 h after co-cultivation with Xoo. Seven types of gene expression patterns of the differential fragments were identified. Ten of 31 sequenced fragments showed homology to genes with known or putative functions involved in metabolism, pathogen response and signaling, while 21 others did not show any homology to sequences with known functions. cDNA-AFLP differential patterns for 5 selected genes were confirmed via real-time reverse transcription–PCR analysis. This work begins to reveal potential disease susceptibility-related genes of this staple crop with global importance.

Key words: rice, Xanthomonas oryzae pv. oryzae, cDNA-AFLP, gene expression profiling, differentially expressed genes

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