钙调节活性氧和内生细菌增强水稻对纹枯病抗性

doi:10.3864/j.issn.0578-1752.2025.11.006

Abstract

Abstract:

【Background】 Rice sheath blight, caused by Rhizoctonia solani, is a prevalent disease in rice-growing regions worldwide, posing significant challenges to the green ecological cultivation of rice due to the severe lack of resistant varieties.【Objective】 To elucidate the regulatory effect of exogenous calcium treatment on rice disease resistance and its regulatory mechanism.【Method】 Four-leaf stage indica and japonica rice seedlings were sprayed with a 5 mmol·L^-1 calcium regulator at 150 mL and sprayed twice at a 24 h interval. The toothpick insertion method was used to inoculate R. solani in the second- and third-leaf sheaths of rice 48 h after the first spray. Leaves from the same part were taken for determination of reactive oxygen species (ROS)-related indicators, photosynthetic pigment content, and endophytic bacterial analysis.【Result】 Calcium addition treatment (CaCl₂ and Ca(NO₃)₂) enhanced the resistance of the tested rice against R. solani while the chelating agent EGTA treatment weakened their resistance. Compared with the blank control, the calcium content of plants was increased with calcium addition treatment and decreased with calcium reduction treatment; pathogen inoculation resulted in lower calcium content in the plants compared to the corresponding control treatments. All calcium addition treatments caused an increase in the content of and H₂O₂ in rice seedlings, the change of ROS content caused by the calcium reduction treatment was more obvious than that of calcium addition treatment. Calcium addition treatment caused changes in rice ROS content consistent with the trend of lesion area changes, with a positive correlation between the two. Calcium addition treatment also maintained the enzyme activities of SOD, POD, CAT, APX, GPX and content of GSH at relatively high levels. The increase in CAT activity was particularly significant. In most cases, calcium reduction treatment significantly reduced the activity of related enzymes. Calcium addition treatment could reconstruct rice’s ROS homeostasis and enhance disease resistance and immune responses, including ROS production, antioxidant systems, and membrane damage indicators. Compared with the blank control, the positive correlation between the lesion area and H₂O₂ content in rice treated with calcium addition was enhanced, the negative correlation with CAT was enhanced, the negative correlation with ASA was enhanced, the negative correlation with APX was weakened, the correlation with GSH turned from the negative to positive, the positive correlation with MDA was weakened, and the correlation with conductivity turned from negative to positive. Calcium addition treatment reduced the microbial richness of endophytic bacterial community in inoculated rice leaves and limitedly increased the diversity of endophytic bacterial community. Although calcium addition treatment had a limited impact on the endophytic bacterial co-occurrence network in rice, the inoculation of R. solani significantly affected the endophytic bacterial network in rice. Furthermore, the network robustness after calcium addition treatment was significantly higher than that of the control group and the calcium reduction treatment group, both before and after inoculation; the network vulnerability after inoculation was significantly lower than that of the control group and the calcium reduction treatment group.【Conclusion】 Calcium addition treatment can significantly reduce the relative lesion area of tested rice, enhance the activities of SOD, POD, CAT and content of GSH, decrease the accumulation of ROS, reduce membrane damage, maintain the homeostasis of ROS and enhance the disease resistance of rice; simultaneously, calcium addition treatment reduces the richness of endophytic bacterial community, increases its diversity, enhances the proportion of beneficial bacterial genera, restructures the endophytic bacterial community with enhanced stability.

Key words: calcium, rice, sheath blight, Rhizoctonia solani, reactive oxygen species (ROS), endophytic bacteria

ZHAO ShouShuai, DU MengXiang, GUO SiYu, ZHANG Shuo, ZHAO HongWei, ZHAO ChangJiang. Calcium Regulates Reactive Oxygen Species and Endophytic Bacteria to Enhance Rice Resistance to Sheath Blight[J].Scientia Agricultura Sinica, 2025, 58(11): 2145-2161.

Figures/Tables 12

Table 1

Fig. 1

Table 2

Fig. 2

Table 3

Table 4

Table 5

Table 6

Table 7

Fig. 3

Fig. 4

Fig. 5

References 66

[1]	LIU Z, ZHU Y, SHI H, QIU J, DING X, KOU Y. Recent progress in rice broad-spectrum disease resistance. The International Journal of Molecular Sciences, 2021, 22(21): 11658.
[2]	YUAN M, JIANG Z, BI G, NOMURA K, LIU M, WANG Y, CAI B, ZHOU J M, HE S Y, XIN X F. Pattern-recognition receptors are required for NLR-mediated plant immunity. Nature, 2021, 592(7852): 105-109.
[3]	NGOU B P M, DING P, JONES J D G. Thirty years of resistance: Zig-zag through the plant immune system. The Plant Cell, 2022, 34(5): 1447-1478. doi: 10.1093/plcell/koac041 pmid: 35167697
[4]	KADOTA Y, LIEBRAND T W H, GOTO Y, SKLENAR J, DERBYSHIRE P, MENKE F L H, TORRES M A, MOLINA A, ZIPFEL C, COAKER G, SHIRASU K. Quantitative phosphoproteomic analysis reveals common regulatory mechanisms between effector- and PAMP-triggered immunity in plants. New Phytologist, 2019, 221(4): 2160-2175. doi: 10.1111/nph.15523 pmid: 30300945
[5]	KÖSTER P, DEFALCO T A, ZIPFEL C. Ca²⁺ signals in plant immunity. The EMBO Journal, 2022, 41(12): e110741.
[6]	MOEDER W, PHAN V, YOSHIOKA K. Ca²⁺ to the rescue-Ca²⁺ channels and signaling in plant immunity. Plant Science, 2019, 279: 19-26.
[7]	BI G, SU M, LI N, LIANG Y, DANG S, XU J, HU M, WANG J, ZOU M, DENG Y, et al. The ZAR1 resistosome is a calcium-permeable channel triggering plant immune signaling. Cell, 2021, 184(13): 3528-3541.e12. doi: 10.1016/j.cell.2021.05.003 pmid: 33984278
[8]	JACOB P, KIM N H, WU F, EL-KASMI F, CHI Y, WALTON W G, FURZER O J, LIETZAN A D, SUNIL S, KEMPTHORN K, REDINBO M R, PEI Z M, WAN L, DANGL J L. Plant “helper” immune receptors are Ca²⁺-permeable nonselective cation channels. Science, 2021, 373(6553): 420-425.
[9]	ZHAO Z X, XU Y J, LEI Y, LI Q, ZHAO J Q, LI Y, FAN J, XIAO S, WANG W M. ANNEXIN 8 negatively regulates RPW8.1-mediated cell death and disease resistance in Arabidopsis. Journal of Integrative Plant Biology, 2021, 63(2): 378-392.
[10]	SUN X, PAN B, WANG Y, XU W, ZHANG S. Exogenous calcium improved resistance to Botryosphaeria dothidea by increasing autophagy activity and salicylic acid level in pear. Molecular Plant-Microbe Interactions, 2020, 33(9): 1150-1160.
[11]	LIANG C, ZHANG Y, REN X. Calcium regulates antioxidative isozyme activity for enhancing rice adaption to acid rain stress. Plant Science, 2021, 306: 110876.
[12]	LIN Y, GU H, JIA X, WANG W, HONG B, ZHANG F, YIN H. Rhizoctonia solani AG1 IA extracellular polysaccharides: Structural characterization and induced resistance to rice sheath blight. International Journal of Biological Macromolecules, 2023, 244: 125281.
[13]	王爱军, 舒新月, 蒋钰琪, 郑爱萍, 李平, 殷得所. 水稻与纹枯病菌互作的分子机制研究进展. 植物保护学报, 2023, 50(1): 11-21.
	WANG A J, SHU X Y, JIANG Y Q, ZHENG A P, LI P, YIN D S. Recent progresses on molecular mechanisms in the interactions between rice and pathogenic fungus Rhizoctonia solani AG1-IA. Journal of Plant Protection, 2023, 50(1): 11-21. (in Chinese)
[14]	潘晓曦, 张淋淋, 关一鸣, 张悦, 王秋霞. 植物内生促生菌多样性及促生作用研究进展. 特产研究, 2024, doi: 10.16720/j.cnki.tcyj.2024.134.
	PAN X X, ZHANG L L, GUAN Y M, ZHANG Y, WANG Q X. Research progress on diversity and growth promoting effects of plant growth promoting endophytes. Special Wild Economic Animal and Plant Research, 2024, doi: 10.16720/j.cnki.tcyj.2024.134. (in Chinese)
[15]	PANDEY S S, JAIN R, BHARDWAJ P, THAKUR A, KUMARI M, BHUSHAN S, KUMAR S. Plant probiotics - Endophytes pivotal to plant health. Microbiological Research, 2022, 263: 127148.
[16]	巫艳, 周云莹, 朱玺燊, 张力敏, JIBRIL S, 杨志兵, 王一, 李成云. 植物内生菌多样性及其病害生防机制研究进展. 云南农业大学学报(自然科学), 2022, 37(5): 897-905.
	WU Y, ZHOU Y Y, ZHU X S, ZHANG L M, JIBRIL S, YANG Z B, WANG Y, LI C Y. Research progress on plant endophyte diversity and its disease biocontrol mechanism. Journal of Yunnan Agricultural University (Natural Science), 2022, 37(5): 897-905. (in Chinese)
[17]	BARGE E G, LEOPOLD D R, ROJAS A, VILGALYS R, BUSBY P E. Phylogenetic conservatism of mycoparasitism and its contribution to pathogen antagonism. Molecular Ecology, 2022, 31(10): 3018-3030.
[18]	BU Z, LI W, LIU X, LIU Y, GAO Y, PEI G, ZHUO R, CUI K, QIN Z, ZHENG H, et al. The rice endophyte-derived α-mannosidase ShAM1 degrades host cell walls to activate DAMP-triggered immunity against disease. Microbiology Spectrum, 2023, 11(3): e0482422.
[19]	ROJAS E C, JENSEN B, JØRGENSEN H J L, LATZ M A C, ESTEBAN P, COLLINGE D B. The fungal endophyte Penicillium olsonii ML37 reduces fusarium head blight by local induced resistance in wheat spikes. Journal of Fungi, 2022, 8(4): 345.
[20]	HUANG Z, CAI X, SHAO C, SHE Z, XIA X, CHEN Y, YANG J, ZHOU S, LIN Y. Chemistry and weak antimicrobial activities of phomopsins produced by mangrove endophytic fungus Phomopsis sp. ZSU-H76. Phytochemistry, 2008, 69(7): 1604-1608.
[21]	PÉREZ-ALONSO M M, GUERRERO-GALÁN C, GONZÁLEZ ORTEGA-VILLAIZÁN A, ORTIZ-GARCÍA P, SCHOLZ S S, RAMOS P, SAKAKIBARA H, KIBA T, LUDWIG-MÜLLER J, KRAPP A, OELMÜLLER R, VICENTE-CARBAJOSA J, POLLMANN S. The calcium sensor CBL7 is required for Serendipita indica- induced growth stimulation in Arabidopsis thaliana, controlling defense against the endophyte and K⁺ homoeostasis in the symbiosis. Plant, Cell and Environment, 2022, 45(11): 3367-3382.
[22]	ZHOU L, LI C, WHITE J F, JOHNSON R D. Synergism between calcium nitrate applications and fungal endophytes to increase sugar concentration in Festuca sinensis under cold stress. PeerJ, 2021, 9: e10568.
[23]	马军韬, 张国民, 邓凌韦, 王永力, 高洪儒, 王南博, 肖明纲, 赵北平, 任洋, 宫秀杰. 水稻纹枯病离体接种鉴定技术因子量化试验. 江苏农业科学, 2022, 50(21): 127-135.
	MA J T, ZHANG G M, DENG L W, WANG Y L, GAO H R, WANG N B, XIAO M G, ZHAO B P, REN Y, GONG X J. Quantitative test for identification of technical factors for rice stripe blight in vitro inoculation. Jiangsu Agricultural Sciences, 2022, 50(21): 127-135. (in Chinese)
[24]	ARNON D I. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology, 1949, 24(1): 1-15.
[25]	WANG F, CHEN F, CAI Y, ZHANG G, WU F. Modulation of exogenous glutathione in ultrastructure and photosynthetic performance against Cd stress in the two barley genotypes differing in Cd tolerance. Biological Trace Element Research, 2011, 144(1/3): 1275-1288.
[26]	孙佳星, 娄琦, 彭雅慧, 玉蓝, 黄文婷, 李琳琳. 根际Ca²⁺对番茄灰霉病抗性的影响. 沈阳农业大学学报, 2021, 52(2): 137-144.
	SUN J X, LOU Q, PENG Y H, YU L, HUANG W T, LI L L. Effect of rhizosphere Ca²⁺induced resistance to Botrytis cinerea in tomato. Journal of Shenyang Agricultural University, 2021, 52(2): 137-144. (in Chinese)
[27]	SPYCHALLA J P, DESBOROUGH S L. Superoxide dismutase, catalase, and alpha-tocopherol content of stored potato tubers. Plant Physiology, 1990, 94(3): 1214-1218. doi: 10.1104/pp.94.3.1214 pmid: 16667819
[28]	MAEHLY A C, CHANCE B. The assay of catalases and peroxidases. Methods of Biochemical Analysis, 1954, 1: 357-424. pmid: 13193536
[29]	MANO J, OHNO C, DOMAE Y, ASADA K. Chloroplastic ascorbate peroxidase is the primary target of methylviologen-induced photooxidative stress in spinach leaves: Its relevance to monodehydroascorbate radical detected with in vivo ESR. Biochimica et Biophysica Acta, 2001, 1504(2/3): 275-287.
[30]	LING F, SU Q, JIANG H, CUI J, HE X, WU Z, ZHANG Z, LIU J, ZHAO Y. Effects of strigolactone on photosynthetic and physiological characteristics in salt-stressed rice seedlings. Scientific Reports, 2020, 10(1): 6183. doi: 10.1038/s41598-020-63352-6 pmid: 32277136
[31]	PETERS R R, BAIER KREPSKY P, SIQUEIRA-JUNIOR J, DA SILVA ROCHA J C, MARQUES BEZERRA M, DE ALBUQUERQUE RIBEIRO R, DE BRUM-FERNANDES A J, ROCHA FARIAS M, CASTRO DA ROCHA F A, RIBEIRO-DO-VALLE R M. Nitric oxide and cyclooxygenase may participate in the analgesic and anti- inflammatory effect of the cucurbitacins fraction from Wilbrandia ebracteata. Life Sciences, 2003, 73(17): 2185-2197.
[32]	BRENNAN T, FRENKEL C. Involvement of hydrogen peroxide in the regulation of senescence in pear. Plant Physiology, 1977, 59(3): 411-416. doi: 10.1104/pp.59.3.411 pmid: 16659863
[33]	KAMPFENKEL K, VAN MONTAGU M, INZÉ D. Extraction and determination of ascorbate and dehydroascorbate from plant tissue. Analytical Biochemistry, 1995, 225(1): 165-167. doi: 10.1006/abio.1995.1127 pmid: 7778771
[34]	LAWRENCE R A, BURK R F. Glutathione peroxidase activity in selenium-deficient rat liver. Biochemical and Biophysical Research Communications, 1976, 71(4): 952-958. doi: 10.1016/0006-291x(76)90747-6 pmid: 971321
[35]	NAGALAKSHMI N, PRASAD M N. Responses of glutathione cycle enzymes and glutathione metabolism to copper stress in Scenedesmus bijugatus. Plant Science, 2001, 160(2): 291-299.
[36]	YAN C, GAO Q, YANG M, SHAO Q, XU X, ZHANG Y, LUAN S. Ca²⁺/calmodulin-mediated desensitization of glutamate receptors shapes plant systemic wound signalling and anti-herbivore defence. Nature Plants, 2024, 10(1): 145-160.
[37]	ZHANG Y, TIAN H, CHEN D, ZHANG H, SUN M, CHEN S, QIN Z, DING Z, DAI S. Cysteine-rich receptor-like protein kinases: Emerging regulators of plant stress responses. Trends in Plant Science, 2023, 28(7): 776-794. doi: 10.1016/j.tplants.2023.03.028 pmid: 37105805
[38]	WANG J, REN Y, LIU X, LUO S, ZHANG X, LIU X, LIN Q, ZHU S, WAN H, YANG Y, et al. Transcriptional activation and phosphorylation of OsCNGC9 confer enhanced chilling tolerance in rice. Molecular Plant, 2021, 14(2): 315-329.
[39]	SRIVASTAVA R K, PANDEY P, RAJPOOT R, RANI A, GAUTAM A, DUBEY R S. Exogenous application of calcium and silica alleviates cadmium toxicity by suppressing oxidative damage in rice seedlings. Protoplasma, 2015, 252(4): 959-975. doi: 10.1007/s00709-014-0731-z pmid: 25413289
[40]	RAHMAN A, NAHAR K, HASANUZZAMAN M, FUJITA M. Calcium supplementation improves Na⁺/K⁺ ratio, antioxidant defense and glyoxalase systems in salt-stressed rice seedlings. Frontiers in Plant Science, 2016, 7: 609.
[41]	GAO Y, DONG X, WANG R, HAO F, ZHANG H, ZHANG Y, LIN G. Exogenous calcium alleviates oxidative stress caused by salt stress in peanut seedling roots by regulating the antioxidant enzyme system and flavonoid biosynthesis. Antioxidants, 2024, 13(2): 233.
[42]	CHE Y, WANG H, YAO T, WANG Z, BO L, ZHANG H. Activation of the antioxidant system and transduction of the mediated by exogenous calcium improve drought resistance in tobacco. Plant Stress, 2024, 13: 100551.
[43]	DE TORRES ZABALA M, LITTLEJOHN G, JAYARAMAN S, STUDHOLME D, BAILEY T, LAWSON T, TILLICH M, LICHT D, BÖLTER B, DELFINO L, TRUMAN W, MANSFIELD J, SMIRNOFF N, GRANT M. Chloroplasts play a central role in plant defence and are targeted by pathogen effectors. Nature Plants, 2015, 1: 15074. doi: 10.1038/nplants.2015.74 pmid: 27250009
[44]	PANDEY P K, SAMANTA R, YADAV R N S. Inside the plant: Addressing bacterial endophytes in biotic stress alleviation. Archives of Microbiology, 2019, 201(4): 415-429. doi: 10.1007/s00203-019-01642-y pmid: 30834947
[45]	TIAN D, CHEN Z, LIN Y, LIANG T, CHEN Z, GUO X, WANG F, WANG Z. The interaction between rice genotype and Magnaporthe oryzae regulates the assembly of rice root-associated microbiota. Rice, 2021, 14(1): 40.
[46]	HERNANDEZ D J, DAVID A S, MENGES E S, SEARCY C A, AFKHAMI M E. Environmental stress destabilizes microbial networks. The ISME Journal, 2021, 15(6): 1722-1734.
[47]	SOLANS M, PELLIZA Y I, TADEY M. Inoculation with native actinobacteria may improve desert plant growth and survival with potential use for restoration practices. Microbial Ecology, 2022, 83(2): 380-392.
[48]	ARORA N K, MISHRA J, SINGH P, FATIMA T. Salt-tolerant plant growth-promoting Pseudomonas atacamensis KSS-6 in combination with organic manure enhances rice yield, improves nutrient content and soil properties under salinity stress. Journal of Basic Microbiology, 2024, 64(6): e2300767.
[49]	HE D, SINGH S K, PENG L, KAUSHAL R, VÍLCHEZ J I, SHAO C, WU X, ZHENG S, MORCILLO R J L, PARÉ P W, ZHANG H. Flavonoid-attracted Aeromonas sp. from the Arabidopsis root microbiome enhances plant dehydration resistance. The ISME Journal, 2022, 16(11): 2622-2632.
[50]	JIMÉNEZ-VÁZQUEZ K R, GARCÍA-CÁRDENAS E, BARRERA- ORTIZ S, ORTIZ-CASTRO R, RUIZ-HERRERA L F, RAMOS- ACOSTA B P, CORIA-ARELLANO J L, SÁENZ-MATA J, LÓPEZ- BUCIO J. The plant beneficial rhizobacterium Achromobacter sp. 5B1 influences root development through auxin signaling and redistribution. The Plant Journal, 2020, 103(5): 1639-1654.
[51]	CAI W, OU F, STAEHELIN C, DAI W. Bradyrhizobium sp. strain ORS278 promotes rice growth and its quorum sensing system is required for optimal root colonization. Environmental Microbiology Reports, 2020, 12(6): 656-666.
[52]	NIU Y, HU X, SONG Y, WANG C, LUO P, NI S, JIAO F, QIU J, JIANG W, YANG S, et al. Blautia coccoides is a newly identified bacterium increased by leucine deprivation and has a novel function in improving metabolic disorders. Advanced Science, 2024, 11(18): e2309255.
[53]	ASAF S, NUMAN M, KHAN A L, AL-HARRASI A. Sphingomonas: From diversity and genomics to functional role in environmental remediation and plant growth. Critical Reviews in Biotechnology, 2020, 40(2): 138-152. doi: 10.1080/07388551.2019.1709793 pmid: 31906737
[54]	WANG P, WEI H, KE T, FU Y, ZENG Y, CHEN C, CHEN L. Characterization and genome analysis of Acinetobacter oleivorans S4 as an efficient hydrocarbon-degrading and plant-growth-promoting rhizobacterium. Chemosphere, 2023, 331: 138732.
[55]	VINCENT C V, BIGNELL D R D. Regulation of virulence mechanisms in plant-pathogenic Streptomyces. Canadian Journal of Microbiology, 2024, 70(6): 199-212.
[56]	LI Y, LIU J, DÍAZ-CRUZ G, CHENG Z, BIGNELL D R D. Virulence mechanisms of plant-pathogenic Streptomyces species: An updated review. Microbiology, 2019, 165(10): 1025-1040.
[57]	STRAUSBAUGH C A. Leuconostoc spp. associated with root rot in sugar beet and their interaction with Rhizoctonia solani. Phytopathology, 2016, 106(5): 432-441.
[58]	CAI Y Y, HUANG F Q, LAO X, LU Y, GAO X, ALOLGA R N, YIN K, ZHOU X, WANG Y, LIU B, SHANG J, QI L W, LI J. Integrated metagenomics identifies a crucial role for trimethylamine-producing Lachnoclostridium in promoting atherosclerosis. NPJ Biofilms and Microbiomes, 2022, 8(1): 11.
[59]	PETERS S, FOHMANN I, RUDEL T, SCHUBERT-UNKMEIR A. A comprehensive review on the interplay between Neisseria spp. and host sphingolipid metabolites. Cells, 2021, 10(11): 3201.
[60]	MIDGETT C R, KULL F J. Structural insights into regulation of Vibrio virulence gene networks. Advances in Experimental Medicine and Biology, 2023, 1404: 269-294.
[61]	LIU Y, WANG H, JIANG H, SUN Z, SUN A. Alloprevotella can be considered as a potential oral biomarker in intestinal metaphase of gastric patients. Studies in Health Technology and Informatics, 2023, 308: 155-167.
[62]	CHEUNG M K, LEUNG T F, TAM W H, LEUNG A S Y, CHAN O M, NG R W Y, YAU J W K, YUEN L Y, TONG S L Y, HO W C S, YEUNG A C M, CHEN Z, CHAN P K S. Development of the early-life gut microbiome and associations with eczema in a prospective Chinese cohort. mSystems, 2023, 8(5): e0052123.
[63]	SEVIAR D, NYUNT K, SAYER C, WRIGHT D, RIDER T. Romboutsia-induced bone marrow necrosis at presentation of acute myeloid leukaemia. British Journal of Haematology, 2022, 196(2): 262.
[64]	SHARMA G, GARG N, HASAN S, SHIRODKAR S. Prevotella: An insight into its characteristics and associated virulence factors. Microbial Pathogenesis, 2022, 169: 105673.
[65]	GUPTA S, PANDEY S, NANDI S P, SINGH M. Modulation of ethylene and ROS-scavenging enzymes by multifarious plant growth-promoting endophytes in tomato (Solanum lycopersicum) plants to combat Xanthomonas-induced stress. Plant Physiology and Biochemistry, 2023, 202: 107982.
[66]	魏松红, 张亚婷, 孔令春, 杨梦冉, 李晶, 郑媛媛, 王海宁, 王妍. 水稻纹枯病生防菌的筛选及田间防治效果. 农药, 2022, 61(7): 532-536.
	WEI S H, ZHANG Y T, KONG L C, YANG M R, LI J, ZHENG Y Y, WANG H N, WANG Y. Screening of biocontrol bacteria for rice sheath blight and field control effect. Agrochemicals, 2022, 61(7): 532-536. (in Chinese)

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

引物（基因号） Primer (Gene ID)	引物序列 Primer sequence (5′-3′)
OsCAT2-F (LOC_Os06g51150)	GCACAGTTTGACAGGGAG
OsCAT2-R (LOC_Os06g51150)	GGTCTGAACACCAGGAGC
OsCNGC9-F (LOC_Os09g38580)	ACTCATGGTGGCCAGAAGAA
OsCNGC9-R (LOC_Os09g38580)	TGCATTTGAGCAATGGCACT
OsAnn8-F (LOC_Os09g20330)	CGATGTGCGACGCCAAGAC
OsAnn8-R (LOC_Os09g20330)	TCGCCGTCCTTCCTCTTCAG
OsMCA1-F (LOC_Os03g06120)	TTGATGTGTTGTTGCTGTGCG
OsMCA1-R (LOC_Os03g06120)	ACTGAAATGAGGGTGGGCTAATC
OsActin-F (LOC_Os10g36650)	CATGCTATCCCTCGTCTCGACCT
OsActin-R (LOC_Os10g36650)	GCACTTCATGATGGAGTTGTAT

处理 Treatment	相对病斑面积Relative lesion area
处理 Treatment	晶两优Jingliangyou	垦粳Kenjing
Con	0.32±0.01b	0.49±0.02b
CaCl₂	0.17±0.01d	0.08±0.01d
Ca(NO₃)₂	0.26±0.01c	0.16±0.01c
EGTA	0.41±0.01a	0.61±0.02a

测定指标 Measurement indicator	处理 Treatment	晶两优Jingliangyou		垦粳Kenjing
测定指标 Measurement indicator	处理 Treatment	BRS	ARS	BRS	ARS
含量 content (nmol·g^-1 FW)	Con	0.80±0.02c	1.55±0.02b**	0.22±0.01c	0.51±0.02b**
	CaCl₂	0.94±0.04b	1.21±0.01c**	0.27±0.01b	0.33±0.02d
	Ca(NO₃)₂	1.02±0.03b	1.26±0.01c**	0.29±0.01b	0.42±0.02c**
	EGTA	1.31±0.01a	1.67±0.06a**	0.43±0.03a	0.82±0.04a**
H₂O₂含量 H₂O₂ content (mmol·g^-1 FW)	Con	0.74±0.01b	1.25±0.02b**	0.58±0.02c	1.25±0.01b**
	CaCl₂	0.77±0.01b	0.88±0.01d**	0.66±0.02b	0.86±0.02d**
	Ca(NO₃)₂	0.77±0.01b	0.96±0.01c**	0.70±0.01b	0.94±0.02c**
	EGTA	0.86±0.02a	1.61±0.02a**	1.04±0.03a	1.43±0.02a**
电解质渗透率 Electrolyte leakage rate (%)	Con	6.20±0.18b	12.15±0.18a**	3.88±0.19b	5.68±0.24b**
	CaCl₂	5.40±0.10c	7.61±0.12c**	3.02±0.16c	3.66±0.13d
	Ca(NO₃)₂	5.28±0.15c	10.18±0.11b**	3.52±0.19bc	4.41±0.20c
	EGTA	7.99±0.22a	11.69±0.15a**	4.57±0.19a	8.60±0.22a**
MDA含量 MDA content (µmol·g^-1 FW)	Con	0.0091±0.0002b	0.0137±0.0001b**	0.0060±0.0001b	0.0090±0.0001b**
	CaCl₂	0.0089±0.0001b	0.0119±0.0002c**	0.0047±0.0001c	0.0056±0.0002d**
	Ca(NO₃)₂	0.0093±0.0002b	0.0118±0.0002c**	0.0060±0.0001b	0.0069±0.0002c**
	EGTA	0.0121±0.0002a	0.0161±0.0001a**	0.0091±0.0001a	0.0113±0.0002a**

测定指标 Measurement indicator	处理 Treatment	晶两优Jingliangyou		垦粳Kenjing
测定指标 Measurement indicator	处理 Treatment	BRS	ARS	BRS	ARS
Ca含量 Ca content (μg·g^-1)	Con	218.51±7.93b	113.55±5.43b**	92.89±6.35c	66.99±3.60b
	CaCl₂	242.76±8.96a	143.31±10.88a**	102.53±10.66b	86.28±2.63a
	Ca(NO₃)₂	233.67±9.17a	136.14±8.02ab**	124.30±6.04a	72.50±4.54b**
	EGTA	189.31±8.84c	160.11±7.90a	86.00±7.57d	71.68±4.51b
SOD活性 SOD activity (U·g^-1 FW)	Con	108.80±1.37c	142.69±1.52c**	140.37±0.93b	166.67±1.26b**
	CaCl₂	146.07±0.90a	172.83±0.91a**	140.89±0.55b	174.28±1.70a**
	Ca(NO₃)₂	149.17±1.02a	169.86±0.26a**	146.19±1.54a	174.02±0.68a**
	EGTA	125.88±1.92b	163.17±1.10b**	136.29±0.63c	131.60±1.99c
POD活性 POD activity (U·mg^-1 FW)	Con	24.58±0.18c	26.94±0.29b**	26.46±0.26c	30.13±0.48c**
	CaCl₂	27.91±0.36b	30.76±0.41a**	31.29±0.13a	34.57±0.30a**
	Ca(NO₃)₂	29.10±0.20a	30.16±0.64a	28.50±0.03b	32.07±0.28b**
	EGTA	22.98±0.20d	26.38±0.44b**	25.67±0.07d	30.88±0.15c**
CAT活性 CAT activity (U·g^-1 FW)	Con	28.89±2.22b	46.67±3.33b	28.89±2.22b	71.11±2.22b**
	CaCl₂	42.22±2.22a	93.33±3.85a**	55.56±2.22a	100.00±3.85a**
	Ca(NO₃)₂	37.78±2.22a	80.00±3.85a**	55.56±2.22a	95.56±2.22a**
	EGTA	22.22±2.22b	33.33±6.67b	22.22±2.22b	68.89±2.22b**

测定指标 Measurement indicator	处理 Treatment	晶两优Jingliangyou		垦粳Kenjing
测定指标 Measurement indicator	处理 Treatment	BRS	ARS	BRS	ARS
APX活性 APX activity (U·g^-1 FW)	Con	70.00±3.21b	84.22±2.25a**	81.48±1.48b	106.67±2.57c**
	CaCl₂	78.89±1.92a	88.07±1.78a	79.26±3.70b	144.44±4.44a**
	Ca(NO₃)₂	83.33±1.92a	89.56±1.90a	97.78±6.67a	133.33±2.57b**
	EGTA	55.56±1.28c	42.22±1.28b**	46.67±2.57c	76.30±2.67d**
GPX活性 GPX activity (U·mg^-1 FW)	Con	1.72±0.02b	2.20±0.01b**	2.27±0.01c	2.34±0.02b**
	CaCl₂	1.93±0.02a	2.38±0.002a**	2.32±0.005b	2.43±0.01a**
	Ca(NO₃)₂	1.91±0.01a	2.15±0.02c**	2.36±0.02a	2.42±0.02a
	EGTA	1.52±0.005c	2.06±0.02d**	2.24±0.01c	2.29±0.01c
ASA含量 ASA content (μg·g^-1 FW)	Con	208.55±3.03a	195.83±4.91a	207.81±0.52a	207.18±0.61a
	CaCl₂	208.63±3.79a	196.35±3.08a	208.90±0.52a	207.41±0.69a
	Ca(NO₃)₂	209.28±2.61a	196.19±3.67a	209.33±0.6a	208.88±0.58a
	EGTA	187.11±3.67b	172.46±1.77b	206.08±0.45b	203.15±0.63b
GSH含量 GSH content (μmol·g^-1 FW)	Con	308.14±4.14c	320.73±4.64c	349.52±3.18ab	370.06±3.60b
	CaCl₂	330.67±4.03b	373.08±5.78b**	360.12±3.44a	383.98±5.74b
	Ca(NO₃)₂	351.21±5.78a	406.21±4.64a**	360.79±5.18a	419.10±10.35a**
	EGTA	333.32±1.75b	296.87±2.89d**	338.68±3.44b	321.27±3.60c

Calcium Regulates Reactive Oxygen Species and Endophytic Bacteria to Enhance Rice Resistance to Sheath Blight

RichHTML

PDF

Abstract

Cite this article

share this article

Figures/Tables 12

References 66

Related Articles 15

Metrics

Comments

Recommended 0

基因 Gene	处理 Treatment	相对表达量Relative expression level
基因 Gene	处理 Treatment	BRS	ARS
CNGC9	Con	1.00±0.04a	0.71±0.05a
	CaCl₂	1.01±0.08a	0.62±0.06a
	Ca(NO₃)₂	1.01±0.08a	0.24±0.03b**
	EGTA	1.03±0.19a	0.74±0.03a
Ann8	Con	1.18±0.18a	0.18±0.02b**
	CaCl₂	1.06±0.22a	0.69±0.13a
	Ca(NO₃)₂	1.08±0.27a	0.08±0.01b**
	EGTA	1.04±0.21a	0.12±0.003b**
MCA1	Con	1.00±0.07a	0.44±0.05a*
	CaCl₂	1.05±0.21a	0.26±0.07a**
	Ca(NO₃)₂	1.04±0.21a	0.32±0.02a**
	EGTA	1.00±0.05a	0.48±0.02a*
CAT2	Con	1.04±0.19a	0.92±0.03c
	CaCl₂	1.11±0.35a	1.83±0.20b*
	Ca(NO₃)₂	1.01±0.08a	2.30±0.03a**
	EGTA	1.01±0.07a	0.58±0.03c

[1]	PENG TingShen, LU JiuYan, WU MeiLin, YAN YuXin, LIU HongZhou, NAN WenBin, QIN XiaoJian, LI Ming, GONG JunYi, LIANG YongShu. QTL Analysis of Yield-Related Traits in Both Huangnuo2^# and Changbai7^# of Perennial Chinese Rice [J]. Scientia Agricultura Sinica, 2026, 59(7): 1361-1379.
[2]	CUI JieHao, ZHANG Meng, WANG Qin, YU JiaYan, LIN Kun, LI ShangZe, LAN Heng, GENG YanQiu, ZHANG Qiang, GUO LiYing, SHAO XiWen. Evaluation of Lodging Resistance and Its Physiological Mechanisms in Japonica Rice Resources [J]. Scientia Agricultura Sinica, 2026, 59(7): 1420-1438.
[3]	YUAN HaoLiang, NIE Jun, LI Peng, LU YanHong, LIAO YuLin, XU ChangXu, LI ZhongYi, CAO WeiDong, ZHANG JiangLin. Effects of Co-Utilization of Chinese Milk Vetch and Rice Straw on Soil Phosphorus Composition and Phosphorus Activation of Paddy Field in Southern China [J]. Scientia Agricultura Sinica, 2026, 59(7): 1480-1491.
[4]	XU YangHaoJun, CHEN LiMing, YANG ShiQi, TANG YiFan, TAN XueMing, ZENG YongJun, PAN XiaoHua, ZENG YanHua. Effects of Long-Term Different Straw Returning Methods on Soil Organic Carbon, Nutrients and Aggregate Formation in Different Soil Layers of Double Cropping Rice Field [J]. Scientia Agricultura Sinica, 2026, 59(7): 1492-1506.
[5]	ZHOU XinJie, REN Hao, CHEN YingLong, ZHANG JiWang, ZHAO Bin, REN BaiZhao, LIU Peng, WANG HongZhang. Effects of Calcium Peroxide on Root Morphology and Yield Formation of Summer Maize in Waterlogging Farmland [J]. Scientia Agricultura Sinica, 2026, 59(6): 1203-1216.
[6]	LI XingYu, HUANG Rong, XIAN YiMing, TIAN JiaoJiao, MA XiaoJin, YANG QiaoXi, LI Bing, WANG ChangQuan. Characteristics of Organic Carbon Fractions and Carbon Dioxide Emissions of Different Size Aggregates in Rice Field Soils in Response to Long-Term Fertilization [J]. Scientia Agricultura Sinica, 2026, 59(6): 1255-1271.
[7]	MA ZhaoHui, QUAN ChengZhe, CHENG HaiTao, YANG KanJie, LI XinRui, LÜ WenYan. The Breeding Goals and Strategies of Northeast Japonica Rice Under the Background of Zhongke Fa No.5 [J]. Scientia Agricultura Sinica, 2026, 59(5): 927-936.
[8]	ZHANG WeiJian, YAN ShengJi, SHANG ZiYin, TANG ZhiWei, WU LiuGe, LI JiaRui, CHEN HaoTian, DENG AiXing, ZHANG Jun, ZHANG Xin, ZHENG ChengYan, SONG ZhenWei. Methane Emissions from Paddy Fields: Not Entirely Attributable to Rice Cultivation [J]. Scientia Agricultura Sinica, 2026, 59(4): 824-833.
[9]	CHEN Min, JIAO ZiLan, QIAO ChengBin, XU Hao, ZHANG Bi, MA DongHua, KONG WeiRu, WANG JingWen, SONG JiaWei, LUO ChengKe, LI PeiFu, TIAN Lei. Morpho-Physiological Responses and Adaptive Strategies of Rice Germplasm Accessions from Different Subspecies Under Salt Stress [J]. Scientia Agricultura Sinica, 2026, 59(4): 705-722.
[10]	GUO FuCheng, TANG HaiJiang, HAO XinYi, MA GuoLin, YANG JiuJu, HUANG LinFeng, TIAN Lei, WANG Bin, LUO ChengKe. Effects of Different Irrigation Methods on Water-Salt Transport, Rice Yield, and Water Use Efficiency in Saline Soil in Ningxia [J]. Scientia Agricultura Sinica, 2026, 59(4): 750-764.
[11]	LUO Wei, YU Hong, YUAN LiXin, WANG LingLing, ZHAO JinPeng, YIN Wei, WANG MingTian, WANG RuLin. Change of Geographic Distributions of Ratoon Rice in Sichuan- Chongqing Under Global Climate Change [J]. Scientia Agricultura Sinica, 2026, 59(4): 765-780.
[12]	ZHU Shu, GUO ZhiPeng, SUN Ying. Functional Analysis of Rice Target of Rapamycin OsTOR in Regulating Root Elongation [J]. Scientia Agricultura Sinica, 2026, 59(3): 475-485.
[13]	LÜ WenYan, CHENG HaiTao, MA ZhaoHui, TIAN ShuHua. Discussion on Hybridization Breeding Technology and Strategy of Rice in the New Era of Breeding [J]. Scientia Agricultura Sinica, 2026, 59(2): 233-238.
[14]	LIAO TingLu, SHI YaFei, XIAO DongHao, SHE YangMengFei, GUO FuCheng, YANG JiuJu, TANG HaiJiang, LUO ChengKe. The Effect of Exogenous Nitroprusside on Sugar Metabolism in Rice Seedlings Under Alkaline Stress [J]. Scientia Agricultura Sinica, 2026, 59(2): 265-277.
[15]	LIU TianSheng, LIU GengYuan, ZHAO AnQi, YANG Xu, CAI MingXue, YANG AiWen, LOU MingXuan, LI MuKai, WANG Han, ZHANG YaLing. Pathogenic Population of Rice Bakanae Disease in Heilongjiang Province [J]. Scientia Agricultura Sinica, 2026, 59(2): 305-321.

测定指标 Measurement indicator	处理 Treatment	晶两优Jingliangyou		垦粳Kenjing
测定指标 Measurement indicator	处理 Treatment	BRS	ARS	BRS	ARS
叶绿素a+b含量 Chlorophyll a+b content (mg·g^-1 FW)	Con	4.08±0.08a	2.99±0.09b**	3.00±0.13b	2.34±0.07b**
	CaCl₂	4.15±0.11a	3.61±0.08a**	3.47±0.08a	2.56±0.09b**
	Ca(NO₃)₂	4.24±0.05a	3.52±0.16a**	3.02±0.09b	2.82±0.07a
	EGTA	3.00±0.12b	2.34±0.09c**	2.47±0.11c	1.96±0.06c**
类胡萝卜素含量 Carotenoid content (mg·g^-1 FW)	Con	0.32±0.01b	0.20±0.02b**	0.23±0.01b	0.19±0.01a
	CaCl₂	0.39±0.01a	0.28±0.01a**	0.32±0.01a	0.20±0.01a**
	Ca(NO₃)₂	0.36±0.01a	0.26±0.01a**	0.25±0.01b	0.20±0.01a**
	EGTA	0.27±0.02c	0.17±0.02b**	0.18±0.01c	0.15±0.003b