Scientia Agricultura Sinica ›› 2025, Vol. 58 ›› Issue (7): 1451-1462.doi: 10.3864/j.issn.0578-1752.2025.07.015

• ANIMAL SCIENCE·VETERINARY SCIENCE • Previous Articles    

Effect of Gln on Endoplasmic Reticulum Stress in Retained Fetal Membranes Cows Under Oxidative Stress via the PI3K/AKT Pathway

WANG Wei(), LUO ChunHai, JIA HongDou, LIU JiaJin, LI DanYang, FU ShiXin()   

  1. College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163000, Heilongjiang
  • Received:2024-12-02 Accepted:2025-02-14 Online:2025-04-08 Published:2025-04-08
  • Contact: FU ShiXin

RichHTML

44

PDF

121

Abstract:

【Objective】This study aimed to determine whether glutamine could alleviate endoplasmic reticulum stress in the maternal placenta of cows with retained fetal membranes (RFM) caused by oxidative stress through the PI3K pathway, and to explore the protective mechanism of glutamine. 【Method】Three cows with RFM and three healthy cows (NRFM) were selected based on whether the fetal membranes could be normally expelled within 12 hours after parturition. The content of glutamine (Gln) in the serum of RFM and NRFM cows was determined. The expression changes of endoplasmic reticulum stress (GRP78, p-PERK, PERK, p-IRE1α, IRE1α, and ATF6) and key apoptosis factors (Caspase-3, Caspase-8, Caspase-9, P53, Bcl-2, and Bax) in the maternal placenta of the two groups of cows were detected by qRT-PCR and Western blot. In the in vitro experiment, bovine uterine caruncle epithelial cells (UCEs) were stimulated with 400 μmol·L-1 H2O2 to establish an in vitro oxidative stress model of bovine UCEs. On this basis, Gln pretreatment, PI3K inhibitor (LY294002) and glutamine co-pretreatment were performed. The concentration changes of Ca2+ in the cytoplasm were detected by immunofluorescence, and the expression changes of endoplasmic reticulum stress and apoptosis-related indicators in bovine UCEs were detected by qRT-PCR and Western blot. 【Result】When cows had RFM, the serum Gln content was significantly decreased (P<0.01), and the expression of endoplasmic reticulum stress-related proteins and genes GRP78 (P<0.01), p-PERK/PERK, p-IRE1α/IRE1α, ATF6 (P<0.05) in the placenta tissue was significantly increased, while the expression of apoptosis-related proteins and genes P53 (P<0.01), Caspase-3, Caspase-8, Caspase-9, Bax/Bcl-2 (P<0.05) was significantly decreased. In the in vitro oxidative stress model, the expression of endoplasmic reticulum stress-related proteins and genes GRP78, p-PERK/PERK, p-IRE1α/IRE1α, ATF6 (P<0.01) and apoptosis-related proteins and genes P53, Caspase-3, Caspase-8, Caspase-9, Bax/Bcl-2 (P<0.01) in UCEs were significantly increased after H2O2 stimulation, and the concentration of Ca2+ in the cytoplasm was significantly increased (P<0.01). However, compared with the Gln protection group pretreated only with glutamine, Gln could not reduce the expression of endoplasmic reticulum stress and apoptosis-related proteins and genes in bovine UCEs when PI3K was inhibited, and the concentration of Ca2+ in the cytoplasm was significantly increased (P<0.01). 【Conclusion】When cows had RFM, the maternal placenta tissue underwent strong endoplasmic reticulum stress and abnormal apoptosis, which led to the damage of normal cell function and was an important cause of the disorder of fetal membrane expulsion. High levels of Gln could regulate the expression of key proteins in endoplasmic reticulum stress through the PI3K/AKT pathway, alleviate the cellular dysfunction caused by endoplasmic reticulum stress, and thereby reduce the placental expulsion disorder caused by endoplasmic reticulum stress induced by oxidative stress.

Key words: retained fetal membranes, oxidative stress, endoplasmic reticulum stress, glutamine, uterine caruncle epithelial

Table 1

Primer sequence"

基因名称Name 引物序列Primer sequence (5'→3') 长度 Length (bp)
β-actin F：CCGCAACCAGTTCGCCAT 180
R：CCCACGTACGAGTCCTTCTG
GRP78 F: GTGCCCACCAAGAAGTCTCA 92
R: GTCAGGGGTCGTTCACCTTC
PERK F: CACAGGGACCTCAAGCCTTC 98
R: TCCTCGTCTTGGTCCATTGC
IRE1α F: GCCATGAGGAATAAGAAGCACC 136
R: TGGCATGGTAGGTGTGTGAG
ATF6 F: TACTTCCAGCAGCACCCAAG 150
R: GCACCACGGTCTGACCTTTA
PI3K F：GGUUAAAGAUCCAGAAGUACA 148
R：UACUUCUGGAUCUUUAACCAU
AKT F：AGGACCTGGAGCAGCGTGAG 116
R：CCTCGCTCTCCAGATTCC
ATF4 F：GCTTAAGCCATGGCGCTTTT 132
R：ATGTTGCGAGGTTTTGGTGC
EIF2α F：ACCACCCTGGAGAGAACAGA 124
R：GTGACCACTTTGGGCTCCAT
CHOP F：CCTCGCTCTCCAGATTCC 96
R：TCCTTCATGCGTTGCTTC
Caspase-3 F：GACAGTGGTGCTGAGGATGACATG 168
R：TTCGCCAGGAAAAGTAACCAGGTG
Caspase-8 F：CCAGAAAGGTGTAGCCGTTGAGAC 144
R：CCCAGCAGAAAGTCAGCCTCATC
Caspase-9 F：GATCAGGCCAGGCAGCTAAT 152
R：CGGCTTTGATGGGTCATCCT
P53 F：CCCTGTCGTCCTTTGTCCC 128
R：AAGGGAAGGGGAATACGTGC
Bcl-2 F：TGTGGATGACCGAGTACCTGAACC 106
R：GCCAGACTGAGCAGTGCCTTC
Bax F：GGCTGGACATTGGACTTCCTTCG 114
R：ATGGTGAGCGAGGCGGTGAG

Fig. 1

Results of determination of serum Gln concentrations in NRFM and RFM cows * indicates significant difference compared with N group, P<0.05. ** indicates extremely significant difference compared with group N, P<0.01. The same as below"

Fig. 2

Expression changes of key factors of ER stress in maternal placentas of RFM cows A, B: The expression of ER stress-related proteins in cow maternal placentae is detected by Western blotting; C: The expression of ER stress-related genes in cow maternal placentae is detected by qRT-PCR"

Fig. 3

Expression changes of key factors of apoptosis-related factors in maternal placentas of RFM cows A, B: The expression of apoptosis-related factors proteins in cow maternal placentae is detected by Western blotting; C: The expression of apoptosis-related factors genes in cow maternal placentae is detected by qRT-PCR"

Fig.4

Regulation of the PI3K/AKT pathway by Gln A, B: The expression of p-PI3K/PI3K and p-AKT/AKT proteins in UCEs is detected by Western blotting; C: The expression of PI3K and AKT genes is detected by qRT-PCR. ** indicates that the difference is extremely significant compared with the control group, P<0.01. ## indicates a very significant difference compared with the PI3K inhibition group, P<0.01"

Fig. 5

Effect of Gln on gene and protein expression in ER stress and apoptosis pathways after inhibition of PI3K/AKT pathway A, B: The expression of PI3K/AKT/PERK/eIF2α/ATF4/CHOP pathway proteins in UCEs was detected by Western blotting; C: The expression of PI3K/AKT/PERK/eIF2α/ATF4/CHOP pathway genes in UCEs was detected by qRT-PCR. * indicates significant difference, P<0.05. ** indicates that the difference is extremely significant, P<0.01. The same as below"

Fig. 6

The expression of ER stress-related factors in oxidative stress state UCEs pretreated with high concentrations of Gln A, B: The expression of ER stress-related proteins in UCEs is detected by Western blotting; C: The expression of ER stress-related genes in UCEs is detected by qRT-PCR. * indicates significant difference, P<0.05. ** indicates that the difference is extremely significant, P<0.01"

Fig. 7

Effect of Gln on the expression of apoptosis-related factors after inhibition of the PI3K/AKT pathway A, B: Western blotting was used to detect the expression of apoptosis-related proteins in UCEs; C: qRT-PCR was used to detect the expression of apoptosis-related genes in UCEs"

Fig. 8

Ca2+ efflux after UECs treated with high Gln concentration under oxidative stress A: Control group; B: ER stress group; C: Gln protection group; D: PI3K inhibition group; E: Fluorescence intensity analysis"

[1]
HOOSHMANDABBASI R, ZERBE H, BAUERSACHS S, DE SOUSA N M, BOOS A, KLISCH K. Pregnancy-associated glycoproteins in cows with retained fetal membranes. Theriogenology, 2018, 105: 158-163.

doi: S0093-691X(17)30462-4 pmid: 28982025
[2]
于程, 汤兆学. 奶牛胎衣不下的病因、临床症状和防治措施. 现代畜牧科技, 2018(3): 63.
YU C, TANG Z X. Etiology, clinical symptoms and prevention measures of retained placenta in dairy cows. Modern Animal Husbandry Science & Technology, 2018(3): 63. (in Chinese)
[3]
GOHARY K, LEBLANC S J. Cost of retained fetal membranes for dairy herds in the United States. Journal of the American Veterinary Medical Association, 252(12): 1485-1489.
[4]
邢菲菲, 罗胜缤, 温小庆, 曹一鸣, 李美娟, 罗春海, 付世新. NEFA经MMPs途径对奶牛子宫内膜上皮细胞Ⅳ型胶原蛋白表达的影响. 中国兽医学报, 2022, 42(11): 2250-2255.
XING F F, LUO S B, WEN X Q, CAO Y M, LI M J, LUO C H, FU S X. Effects of NEFA on expression of type Ⅳ collagen in endometrial epithelial cells of dairy cows via MMPs pathway. Chinese Journal of Veterinary Science, 2022, 42(11): 2250-2255. (in Chinese)
[5]
刘炳琦, 罗春海, 姚伟佳, 王薇, 刘佳金, 李丹阳, 付世新. miRNA-424-5p靶向VEGFA对胎衣不下奶牛胎盘胶原蛋白降解的影响. 中国农业科学, 2024, 57(15): 3083-3092. doi: 10.3864/j.issn.0578-1752.2024.15.013.
LIU B Q, LUO C H, YAO W J, WANG W, LIU J J, LI D Y, FU S X. Mechanism of miRNA-424-5p regulate the decomposition of collagen on retained fetal membranes of dairy cows through VEGFA pathway. Scientia Agricultura Sinica, 2024, 57(15): 3083-3092. doi: 10.3864/j.issn.0578-1752.2024.15.013. (in Chinese)
[6]
张国华, 吕思文, 金振华, 王丽坤, 张莹, 鹿凌岩, 李烬, 张备. 奶牛胎衣不下的病因、临床症状和防治措施. 现代畜牧科技, 2021, 40(10): 84-85.
ZHANG G H, LÜ S W, JIN Z H, WANG L K, ZHANG Y, LU L Y, LI J, ZHANG B. The etiology,clinical symptoms,and prevention and treatment measures of retained placenta in dairy cows. Modern Animal Husbandry Science & Technology, 2021, 40(10): 84-85. (in Chinese)
[7]
KANKOFER M. Antioxidative defence mechanisms against reactive oxygen species in bovine retained and not-retained placenta: Activity of glutathione peroxidase, glutathione transferase, catalase and superoxide dismutase. Placenta, 2001, 22(5): 466-472.

pmid: 11373157
[8]
HAVAJNEH F M F. Antioxidants in dairy cattle health and disease. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 2014: 104-109.
[9]
赵璧忱. RFM奶牛血清代谢组学分析及Gln对奶牛RFM发生调控机制的研究[D]. 大庆: 黑龙江八一农垦大学, 2020.
ZHAO B C. Serum metabolomic analysis of RFM dairy cows and study on the regulatory mechanism of Gln on RFM in dairy cows[D]. Daqing: Heilongjiang Bayi Agricultural University, 2020. (in Chinese)
[10]
庄敏, 谢钱龙, 檀灵芳, 庄捷. 橙皮素通过ROS介导内质网应激诱导吉非替尼耐药NCI-H1975细胞发生凋亡. 中国临床药理学与治疗学, 2024, 29(11): 1220-1231.
ZHUANG M, XIE Q L, TAN L F, ZHUANG J. Hesperetin induces apoptosis in gefitinib-resistant NCI-H1975 cells through ROS mediated endoplasmic reticulum stress. Chinese Journal of Clinical Pharmacology and Therapeutics, 2024, 29(11): 1220-1231. (in Chinese)
[11]
张雨欣, 周小杰, 于浩然, 董婉玉, 杨永春, 王晓杜, 周莹珊, 宋厚辉. 内质网应激与氧化应激. 农业生物技术学报, 2022, 30(10): 2009-2024.
ZHANG Y X, ZHOU X J, YU H R, DONG W Y, YANG Y C, WANG X D, ZHOU Y S, SONG H H. Endoplasmic reticulum stress and oxidative stress. Journal of Agricultural Biotechnology, 2022, 30(10): 2009-2024. (in Chinese)
[12]
MING S X, TIAN J, MA K, PEI C B, LI L, WANG Z Y, FANG Z Y, LIU M, DONG H, LI W J, ZENG J W, PENG Y H, GAO X F. Oxalate-induced apoptosis through ERS-ROS-NF-κB signalling pathway in renal tubular epithelial cell. Molecular Medicine, 2022, 28(1): 88.

doi: 10.1186/s10020-022-00494-5 pmid: 35922749
[13]
LIU J, ZONG C G, YU X, DING Y, CHANG B, WANG R Y, SANG L X. Alanyl-glutamine (Ala-gln) ameliorates dextran sulfate sodium (DSS)-induced acute colitis by regulating the gut microbiota, PI3K-Akt/NF-κB/STAT3 signaling, and associated pulmonary injury. ACS Infectious Diseases, 2023, 9(4): 979-992.

doi: 10.1021/acsinfecdis.3c00014 pmid: 36917734
[14]
孙汇蕾, 仲庆振, 李明晔, MOLAWA M N. 热休克蛋白70上调表达对热应激猪小肠上皮细胞凋亡的影响. 动物营养学报, 2020, 32(4): 1867-1874.

doi: 10.3969/j.issn.1006-267x.2020.04.046
SUN H L, ZHONG Q Z, LI M Y, N M. Effects of up-regulated expression of heat shock protein 70 on apoptosis in intestinal epithelial cells of heat-stressed piglets. Chinese Journal of Animal Nutrition, 2020, 32(4): 1867-1874. (in Chinese)
[15]
马焦. Hsp70通过调控p38MAPK信号介导细胞内钙超载与凋亡参与原代大鼠心肌细胞氧糖剥夺: 复氧损伤[D]. 苏州: 苏州大学, 2019.
MA J. Hsp70 participates in oxygen-glucose deprivation and reoxygenation injury in primary rat cardiomyocytes by mediating intracellular calcium overload and apoptosis through p38MAPK signaling pathway[D]. Suzhou: Soochow University, 2019. (in Chinese)
[16]
TODOROVA V, VANDERPOOL D, BLOSSOM S, NWOKEDI E, HENNINGS L, MRAK R, KLIMBERG V S. Oral glutamine protects against cyclophosphamide-induced cardiotoxicity in experimental rats through increase of cardiac glutathione. Nutrition, 2009, 25(7/8): 812-817.
[17]
葛亚楠, 陈建军, 吴腾飞, 郑振东. 尪五味子乙素通过PI3K/Akt信号通路调控内质网应激促进胃癌细胞凋亡. 解剖科学进展, 2022, 28(6): 755-758.
GE Y N, CHEN J J, WU T F, ZHENG Z D. Schisandra B regulates endoplasmic reticulum stress and promotes gastric cancer cell apoptosis through PI3K/Akt signaling pathway. Progress of Anatomical Sciences, 2022, 28(6): 755-758. (in Chinese)
[18]
邵帅. 黄芪甲苷通过介导PI3K/AKT/Nrf2信号通路和抑制内质网应激对脂多糖诱导的MC3T3-E1细胞损伤的保护作用[D]. 锦州: 锦州医科大学, 2022.
SHAO S. Protection of Astragaloside IV on LPS-induced MC3T3-E1 cells injury by mediating PI3K/AKT/Nrf2 signaling pathway and inhibiting endoplasmic reticulum stress[D]. Jinzhou: Jinzhou Medical University, 2022. (in Chinese)
[19]
ZHENG C Y, ZOU X, ZHAO B C, ZHANG M L, LIN H J, LUO C H, XU Z M, SHAO L Y, FU S X. miRNA-185 regulates retained fetal membranes of cattle by targeting STIM1. Theriogenology, 2019, 126: 166-171.
[20]
HOLEČEK M. Origin and roles of alanine and glutamine in gluconeogenesis in the liver, kidneys, and small intestine under physiological and pathological conditions. International Journal of Molecular Sciences, 2024, 25(13): 7037.
[21]
COQUEIRO A Y, ROGERO M M, TIRAPEGUI J. Glutamine as an anti-fatigue amino acid in sports nutrition. Nutrients, 2019, 11(4): 863.
[22]
LIN M Y, LING P Y, HE Q W, CHEN D Z, ZHENG L S, TANG L S, JIANG S W. Low acrosin activity is associated with decreased Spam1/acrosin expression and GSH deficiency-caused premature acrosome release of human sperm cells. Cell and Tissue Research, 2023, 394(3): 529-545.

doi: 10.1007/s00441-023-03826-x pmid: 37833433
[23]
LIU T, SUN L, ZHANG Y B, WANG Y L, ZHENG J. Imbalanced GSH/ROS and sequential cell death. Journal of Biochemical and Molecular Toxicology, 2022, 36(1): e22942.
[24]
张勇涛, 李霞, 李晓齐, 买丽亚木古丽·阿布都克尤木, 张贵程, 齐鲁. 内质网应激通路相关蛋白IRE1、PERK、ATF6在舌鳞癌中的表达及其功能的生物信息学分析. 生物化工, 2023, 9(5): 1-6, 18.
ZHANG Y T, LI X, LI X Q, MAILIYAGULIMU·ABUDUKEYOUMU, ZHANG G C, QI L. Bioinformatics analysis of the expression and function of endoplasmic reticulum stress pathway-related proteins IRE1, PERK and ATF6 in squamous carcinoma of the tongue. Biological Chemical Engineering, 2023, 9(5): 1-6, 18. (in Chinese)
[25]
LIN R J, MA M J, HAN B, ZHENG Y, WANG Y P, ZHOU Y N. Esophageal cancer stem cells reduce hypoxia-induced apoptosis by inhibiting the GRP78-perk-eIF2α-ATF4-CHOP pathway in vitro. Journal of Gastrointestinal Oncology, 2023, 14(4): 1669-1693.
[26]
ZHANG B H, WAN S S, LIU H, QIU Q M, CHEN H, CHEN Z Y, WANG L, LIU X H. Naringenin alleviates renal ischemia reperfusion injury by suppressing ER stress-induced pyroptosis and apoptosis through activating Nrf2/HO-1 signaling pathway. Oxidative Medicine and Cellular Longevity, 2022, 2022: 5992436.
[27]
LI J Y, YANG K Y, DAI S S, HE S Y, LIU R R, GUO Q Y, LIU F. ERS mediated by GRP-78/PERK/CHOP signaling is involved in fluoride-induced ameloblast apoptosis. Biological Trace Element Research, 2024, 202(3): 1103-1114.
[28]
MICHALAK M. Calreticulin: Endoplasmic reticulum Ca2+ gatekeeper. Journal of Cellular and Molecular Medicine, 2024, 28(5): e17839.
[29]
姚伟佳, 罗春海, 刘佳金, 王薇, 李丹阳, 刘炳琦, 付世新. 过表达miRNA-424-5p靶向AKT3对奶牛子宫内膜上皮细胞凋亡的影响. 中国畜牧兽医, 2024, 51(8): 3635-3642.

doi: 10.16431/j.cnki.1671-7236.2024.08.040
YAO W J, LUO C H, LIU J J, WANG W, LI D Y, LIU B Q, FU S X. Effect of overexpression of miRNA-424-5p targeting AKT3 on apoptosis of endometrial epithelial cells in dairy cows. China Animal Husbandry & Veterinary Medicine, 2024, 51(8): 3635-3642. (in Chinese)
[30]
MIYAZAKI T, KANATSU-SHINOHARA M, OGONUKI N, MATOBA S, OGURA A, YABE-NISHIMURA C, ZHANG H L, POMMIER Y, TRUMPP A, SHINOHARA T. Glutamine protects mouse spermatogonial stem cells against NOX1-derived ROS for sustaining self-renewal divisionin vitro. Development, 2023, 150(20): dev201157.
[31]
周乐, 常晨城, 王宇, 满达, 石彩霞, 张文广. PI3K/Akt/mTOR 信号通路调控牛细胞生长作用的研究进展. 饲料研究, 2022, 45(9): 138-142.
ZHOU L, CHANG C C, WANG Y, MAN D, SHI C X, ZHANG W G. Research progress of PI3K/Akt/mTOR signaling pathway in regulating the growth of bovine cells. Feed Research, 2022, 45(9): 138-142. (in Chinese)
[1] ZHANG Bing, YANG YanYan, FENG QianHui, SHI Wen, FANG YiZhen, HUANG JiaBao, SHI DeShun. Effects of Sodium Selenite on the in vitro Maturation of Porcine Oocytes and Their Embryonic Development Potentials [J]. Scientia Agricultura Sinica, 2024, 57(17): 3482-3493.
[2] LIU BingQi, LUO ChunHai, YAO WeiJia, WANG Wei, LIU JiaJin, LI DanYang, FU ShiXin. Mechanism of miRNA-424-5p Regulate the Decomposition of Collagen on Retained Fetal Membranes of Dairy Cows Through VEGFA Pathway [J]. Scientia Agricultura Sinica, 2024, 57(15): 3083-3092.
[3] CUI HongJie, LU ChunTing, PAN LiQin, HU Hui, ZHONG PeiYun, ZHU JieYing, ZHANG KaiZhao, HUANG XiaoHong. Curcumin Alleviates Zearalenone-Induced Oxidative Damage in Porcine Renal Epithelial Cells via SIRT1/FOXO1 Pathway [J]. Scientia Agricultura Sinica, 2023, 56(5): 1007-1018.
[4] XI MengXue, SHEN Dan, SHI YiFan, LI ChunMei. Effects of TBHQ on Pyroptosis, Necroptosis and Inflammatory Damage of Chicken Embryonic Lung Tissues Induced by PM2.5 from Chicken Houses [J]. Scientia Agricultura Sinica, 2023, 56(4): 779-787.
[5] TAO WenJing, ZHANG ZiTing, LIU Yuan, SONG Dan, LI XiangChen. Inhibitory Effect of N-acetylcysteine on Bisphenol A-Induced Apoptosis and Inflammatory Response in Porcine Kidney Cells [J]. Scientia Agricultura Sinica, 2023, 56(3): 549-558.
[6] WANG XiaoHong, XING MingJie, GU XianHong, HAO Yue. Screening of Anti-Apoptotic Protein GRP94 Interaction Proteins in Porcine Hepatic Stellate Cells by Immunoprecipitation Combined with LC-MS/MS [J]. Scientia Agricultura Sinica, 2023, 56(15): 3020-3031.
[7] ZHANG Nan, HAN GuangJie, LIU Qin, LI ChuanMing, QI JianHang, LU YuRong, XIA Yang, XU Jian. Response and Function of the Transcription Factor CncC in Cnaphalocrocis medinalis Infected with Baculovirus CnmeGV [J]. Scientia Agricultura Sinica, 2023, 56(13): 2491-2503.
[8] YANG ChangPei,WANG NaiXiu,WANG Kai,HUANG ZiQing,LIN HaiLan,ZHANG Li,ZHANG Chen,FENG LuQiu,GAN Ling. Effects and Mechanisms of Exogenous GABA Against Oxidative Stress in Piglets [J]. Scientia Agricultura Sinica, 2022, 55(17): 3437-3449.
[9] JIANG ChunHui,SUN XuDong,TANG Yan,LUO ShengBin,XU Chuang,CHEN YuanYuan. Curcumin Alleviates H2O2-Induced Oxidative Stress in Bovine Mammary Epithelial Cells Via the Nrf2 Signaling Pathway [J]. Scientia Agricultura Sinica, 2021, 54(8): 1787-1794.
[10] LÜ ChuYang,DENG PingChuan,ZHANG XiaoLi,SUN YuChao,LIANG WuSheng,HU DongWei. Transcriptomic Analysis of Sclerotia Formation Induced by Low Temperature in Villosiclava virens [J]. Scientia Agricultura Sinica, 2020, 53(22): 4571-4583.
[11] ZHU JingJing,ZHOU XiaoLong,WANG Han,LI XiangChen,ZHAO AYong,YANG SongBai. Prediction and Verification of MicroRNAs Targeting Porcine Endoplasmic Reticulum Stress Pathway [J]. Scientia Agricultura Sinica, 2020, 53(15): 3169-3179.
[12] LI HanTong,JIA ChengLi,ZHANG ShuWen,LU Jing,PANG XiaoYang,LIU Lu,LÜ JiaPing. Chromium (III) Stress Alleviation by Sulfur Compounds During Chromium Bio-enrichment by Saccharomyces cerevisiae [J]. Scientia Agricultura Sinica, 2019, 52(6): 1078-1089.
[13] DU Jiao, WANG YaBo, LI XueHua, HUANG ZhiQiang, YANG YuHeng, BI ChaoWei, YU Yang. Function analysis ofγ-glutamyl phosphate reductase-encoded gene SsGPR1 in Sclerotinia sclerotiorum [J]. Scientia Agricultura Sinica, 2018, 51(19): 3694-3703.
[14] HE QingYun, LIU XiaoWei, JIAO YuBing, YU FangFei, SHEN LiLi, YANG JinGuang, WANG FengLong . Targeted Mutagenesis of NbbZIP28 and Its Stress Response to Virus Infection in Nicotiana benthamiana [J]. Scientia Agricultura Sinica, 2018, 51(14): 2689-2699.
[15] TANG Qin, DENG Yuan-yuan, ZHANG Rui-fen, ZHANG Yan, ZHANG Ming-wei, WEI Zhen-cheng, TANG Xiao-jun, LIU Lei, TI Hui-hui, MA Yong-xuan. Effect of Momordica charantia Fruit Aqueous Extract on Serum Lipids Metabolic Disorder in Restraint Mice [J]. Scientia Agricultura Sinica, 2014, 47(16): 3300-3307.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd