Scientia Agricultura Sinica ›› 2021, Vol. 54 ›› Issue (17): 3573-3586.doi: 10.3864/j.issn.0578-1752.2021.17.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genome-Wide Identification of WOX Family and Expression Analysis of Callus Induction Rate in Tartary Buckwheat

HOU SiYu1(),WANG XinFang1,DU Wei1,FENG JinHua1,HAN YuanHuai1,LI HongYing1,LIU LongLong2,SUN ZhaoXia1()   

  1. 1College of Agronomy, Shanxi Agricultural University, Taigu 030801, Shanxi
    2Center for Agricultural Genetic Resources Research, Shanxi Agricultural University, Taiyuan 030031
  • Received:2021-02-18 Accepted:2021-05-08 Online:2021-09-01 Published:2021-09-09
  • Contact: ZhaoXia SUN E-mail:bragren123@163.com;18636071356@163.com

RichHTML

78

PDF

1839

Abstract:

【Objective】 This study aimed to identify the whole genome WOX (WUSCHEL-related home obox) gene family in Tartary buckwheat and reveal the correlation with sequence characteristics of its gene family members, gene expression pattern and the rate of callus induction. It provides a theoretical basis for breaking through the regeneration and genetic transformation problem of Tartary buckwheat. 【Method】 The protein and nucleic acid sequence of the WOX gene family members in Tartary buckwheat were obtained by homology blast and the sequence of Arabidopsis WOX genes were served as reference. Based on protein homology and conserved domain analysis, all members of Tartary buckwheat WOX gene family were identified. The TBtools software was used to further demonstrate the characteristics of the WOX genes in Tartary buckwheat, including gene structure, conserved domain and cis-acting element. Genomic collinearity of WOX gene family members between Tartary buckwheat and Arabidopsis thaliana was analysed. Based on proximity method, the MEGA X software was used to perform phylogenetic tree of these WOX genes in Tartary buckwheat, Arabidopsis and rice. The hypocotyl explants of 70 Tartary buckwheat varieties were cultured with MS+2,4-D 3.0 mg·L -1+6-BA 1.0 mg·L-1 for callus induction and the callus emergence rate of different genotypes was evaluated. The FtWOX gene expression level was performed by qPCR to compare the different Tartary buckwheat varieties with high and low callus yield. The correlation between callus rate and FTWOXS gene family members was analysed based on Pearson correlation coefficient. 【Result】 A total of 30 WOX genes were identified in Tartary buckwheat and they were unevenly distributed on 8 chromosomes. The 30 Tartary buckwheat WOX genes could be divided into three groups by phylogenetic tree. The WOX genes contained different conserved domains in different groups, and the main conserved domains were HD(Homeodomain), START and MEKHLA. The conserved motif analysis showed that the conserved motif number of FtWOX genes may contain 2 to 10 motifs, and the gene structure analysis showed that the number of exons contained in the genes between 2 to 18. Promoter elements analysis showed 26 different kinds of cis-acting elements in the 30 WOX genes. The phylogenetic analysis showed that 30 Tartary buckwheat, 15 Arabidopsis thaliana and 12 rice WOX gene family members could be divided into three categories, of which the third group is unique to Tartary buckwheat. The collinearity analysis showed that six WOX genes were genomic collinearity between Tartary buckwheat and Arabidopsis thaliana. Expression pattern and correlation analysis show that the expression level of FtWOX1/FtWOX12/FtWOX22/FtWOX23/ FtWOX24 has positive correlation with the callus induction. 【Conclusion】 Collectively, these data suggest that the Tartary buckwheat FtWOX members showed abundant sequence variation characteristics. The expression level and callus rate of WOX gene in different Tartary buckwheat genotypes were significantly different and correlated to some extent, suggesting that different Tartary buckwheat WOX genes had potential functional diversity.

Key words: Tartary buckwheat, WOX gene family, callus induction, gene expression

Table 1

The varieties used for callus induction"

来源Origin 品种Variety name
中国湖北Hubei, China ZNQ171, ZNQ164, ZNQ165, ZNQ166, ZNQ167, ZNQ168, ZNQ169, ZNQ170
中国贵州Guizhou, China ZNQ183, ZNQ184, ZNQ185, ZNQ186, ZNQ187, ZNQ188, ZNQ189
中国广西Guangxi, China ZNQ194, ZNQ195, ZNQ196, ZNQ197, ZNQ198
中国安徽Anhui, China ZNQ161, ZNQ162, ZNQ163
中国云南Yunnan, China ZNQ192, ZNQ190, ZNQ191
中国甘肃Gansu, China ZNQ154, ZNQ155, ZNQ156
中国青海Qinghai, China ZNQ158, ZNQ159
中国湖南Hunan, China ZNQ176, ZNQ177, ZNQ178
美国USA PI647612, PI476852, PI658431, PI658429, PI658439, PI647613
不丹Bhutan PI481673, PI481675, PI481658, PI481672
尼泊尔Nepal PI427240, PI481666, PI658430
未知Unknown ZNQ172, ZNQ174, ZNQ180, PI673868, PI673869, PI673870, PI673874, PI673858, PI673876, PI673845, PI673856, PI673857, PI673855, PI673862, PI673854, PI673846, PI673873, PI673863, PI673847, PI481673, PI673865, PI658438, PI673852

Table 2

The primer sequence for qRT-PCR"

引物名称
Primer name		 正向引物
Forward sequences (5'-3')		 反向引物
Revered sequences (5'-3')		 扩增长度
Amplification length (bp)
FtWOX1 CAGGCGGTGTCTTCTCGATT AACTGACCGGAAGATGGGTG 94
FtWOX2 GATGAAGGACGACGAGCCAG AGAACTCGGTAGGGTTGCAG 137
FtWOX3 CGAGGGGAACGATAAGGTGG GACTCAACTTTGCCATGCTCC 142
FtWOX4 CTCAAGCGCCTTACTCCCTC GCTTTCAAACGCCCTACTCG 102
FtWOX5 AGGACCTGCAAATGGGAAGC TCTGAGACGGCTGCTACTCT 138
FtWOX6 AAGGTGCTCTAATGGCCGC TTGGTCAACGCTTAAACGCC 137
FtWOX7 TTCAAGTCCAAGCCCTGCAA CTCCGCTGTCGATCCATCTC 108
FtWOX8 ACACGACTCTCTCCTGCTTG TGTCGTTGGACTGTTGTGGT 142
FtWOX9 AGTGGACAGTTCGTTGCTGA GGACTCCTGACCGTCGTAAC 133
FtWOX10 ACTACATTGGCACCTGGTCG CCACTAAGCTGCCATCCTCC 124
FtWOX11 AGGCGTGCTAGATTGAAGAGG CTTGTGGCTGGGAATCCTCG 124
FtWOX12 AGAGGACCACCATAGACTCG ACTGAACCGGACCCTCATTC 98
FtWOX13 ACCTTCCAATCAGTTCGCGG ACTTCAAGTTGCTCGTCGGT 117
FtWOX14 GGTGGAACCCTACGCAAGAG CTGAGTTGCGTCGTGATGTG 106
FtWOX15 GGTAGTCCGACTTGAAGGGC CAACCGCCAACTGCATTCTC 111
FtWOX16 AGGCGTGCTAGATTGAAGAGG CTTGTGGCTGGGAATCCTCG 124
FtWOX17 CCTCGAGAAGAGCTTCGACA AACCAGATAGCAACCTGCCG 102
FtWOX18 ACCAAGCTTGAACCACGTCA AGCTGCTTCGACTTCCATCG 113
FtWOX19 ACGGAGATGGAGTGCGAGTA GTAGAGCTCGGCACAGTCAA 128
FtWOX20 TCTCACAGAGCAGAATCGCTG GTCCTTGAATGGCAGGAGGC 129
FtWOX21 GCGGCATTGCGTCATCTAAG TTCAACCTCTGGCTGAGTGC 107
FtWOX22 TCTTGCCCGTTGGATCACTC GCAACACAAGATGGCATCCG 127
FtWOX23 CGTGATTGCCGAGCTGTAGA AGCCGATGCCAAAGTCGTAA 108
FtWOX24 TGAGGAGAAGTCTCCAGGGT CCGGTTCAGGCCTCATCATA 104
FtWOX25 CGTGAACAGGAGATGCCGAT TACTGAACTTGCGTCGGTGT 120
FtWOX26 TGTGAAGAAAGCTCCCTCGG TTCTGTATTTGCTGCGCCGT 111
FtWOX27 CATACGATGACTCTCCAGGGC ATCTAGCGGCGGATGTTCG 116
FtWOX28 TCAGCGATGAGCAGATCAGG CATCTGGCTCGTCGGTTCTG 145
FtWOX29 AAGCTTGCTCGTGAGCTTGG ATGGAGACGGCATCGAACTC 134
FtWOX30 TACGCTCCGGTTGACATTCC AGGCAACAGAGAGAAGCGAC 130
FtHis ATCGACTGGAGGAAAGGCTC GCGGTATCTGTGGGACTTCT 106

Fig. 1

The distribution location of FtWOXs on chromosomes"

Fig. 2

FtWOXs gene family Phylogenetic analysis (A), conserved domain (B) and gene structure analysis (C) in Tartary buckwheat"

Fig. 3

The phylogenetic tree of WOX protein in Arabidopsis thaliana (At), Oryza sativa (Os) and Fagopyrum tataricum (Ft) AtWOX1-13C and AtWUX: The WOX protein of Arabidopsis thaliana; OsWOX3-12B, OsWUX and OsNS1-2: The WOX protein of rice; FtWOX1-30: The WOX protein of Tartary buckwheat, numbers in brackets are the corresponding protein ID"

Fig. 4

The analysis of FtWOXs gene promoter elements in Tartary buckwheat"

Fig. 5

Collinearity analysis of WOX gene family between Fagopyrum tataricum (Ft) and Arabidopsis thaliana (At) "

Fig. 6

The callus growth situation (A) and cluster diagram of induction rate (B) of different Tartary buckwheat varieties Ⅰ: The varieties with callus induction rate of 92% to 100%;Ⅱ: The varieties with callus induction rate of 66% to 78%; Ⅲ, Ⅳ: The varieties with the callus induction rate of 30% to 64% and less than 30%, respectively"

Fig. 7

The heat map of gene expression pattern of FtWOXs in callus among different Tartary buckwheat varieties In black box, the expression tendency of five genes was consistent with inducing rate"

[1] JOSHI D C, CHAUDHARI G V, SOOD S, KANT L, PATTANAYAK A, ZHANG K X, FAN Y, JANOVSKA D, MEGLIC V, ZHOU M L. Revisiting the versatile buckwheat: Reinvigorating genetic gains through integrated breeding and genomics approach. Planta, 2019, 250: 783-801.
doi: 10.1007/s00425-018-03080-4
[2] GIMÉNEZ-BASTIDA J A, ZIELIŃSKI H. Buckwheat as a functional food and its effects on health. Journal of Agricultural and Food Chemistry, 2015, 63(36): 7896-7913.
doi: 10.1021/acs.jafc.5b02498
[3] CAMPBELL C. Buckwheat crop improvement. Fagopyrum, 2003, 20: 1-6.
[4] 王兴春, 李宏, 王敏, 杨致荣. 植物体细胞胚胎发生的调控网络. 生物工程学报, 2010, 26(2): 141-146.
WANG X C, LI H, WANG M, YANG Z R. Regulatory networks of somatic embryogenesis in plant. Chinese Journal of Biotechnology, 2010, 26(2): 141-146. (in Chinese)
[5] GENRING W J, AFFOLTER M, BURGLIN T. Homeodomain proteins. Annual Review of Biochemistry, 1994, 63: 487-526.
doi: 10.1146/annurev.bi.63.070194.002415
[6] DERELLE R, LOPEZ P, GUYADER H L, MANUEL M. Homeodomain proteins belong to the ancestral molecular toolkit of eukaryotes. Evolution & Development, 2007, 9(3): 212-219.
[7] LAUX T, MAYER K F, BERGER J, JURGENS G. The WUSCHEL gene is required for shoot and floral meristem integrity in Arabidopsis. Development, 1996, 122(1): 87-96.
doi: 10.1242/dev.122.1.87
[8] GRAAFF E, LAUX T, RENSING S A. The WUS homeobox- containing (WOX) protein family. Genome Biology, 2009, 10: 248.
doi: 10.1186/gb-2009-10-12-248
[9] ISABEL B, THOMAS L. Regulation of WUSCHEL transcription in the stem cell niche of the Arabidopsis shoot meristem. The Plant Cell, 2005, 17(8): 2271-2280.
doi: 10.1105/tpc.105.032623
[10] MENG W J, CHENG Z J, SANG Y L, ZHANG M M, RONG X F, WANG Z W, TANG Y Y, ZHANG X S. Type-B Arabidopsis response regulators specify the shoot stem cell niche by dual regulation of WUSCHEL. The Plant Cell, 2017, 29(6): 1357-1372.
doi: 10.1105/tpc.16.00640
[11] ZUO J R, NIU Q W, GIOVANNA F, CHUA N H. The WUSCHEL gene promotes vegetative to embryonic transition in Arabidopsis. The Plant Journal, 2002, 30(3): 349-359.
doi: 10.1046/j.1365-313X.2002.01289.x
[12] GAMBINO G, MINUTO M, BOCCACCI P, PERRONE I, VALLANIA R, GRIBAUDO I. Characterization of expression dynamics of WOX homeodomain transcription factors during somatic embryogenesis in Vitis vinifera. Journal of Experimental Botany, 2011, 62(3): 1089-1101.
doi: 10.1093/jxb/erq349
[13] NARDMANN J, WERR W. The invention of WUS-like stem cell-promoting functions in plants predates leptosporangiate ferns. Plant Molecular Biology, 2012, 78: 123-134.
doi: 10.1007/s11103-011-9851-4
[14] BREUNINGER H, RIKIRSCH E, HERMANN M, UEDA M, LAUX T. Differential expression of WOX genes mediates apical- basal axis formation in the Arabidopsis embryo. Cell, 2008, 14(6): 867-876.
[15] ZHU J, SHI H, LEE B H, DAMSZ B, CHENG S, STIRM V, ZHU J K, HASEGAWA P M, BRESSAN R A. An Arabidopsis homeodomain transcription factor gene, HOS9, mediates cold tolerance through a CBF-independent pathway. Proceedings of the National Academy of Sciences of the United States of America, 2004, 101(26): 9873-9878.
[16] CHENG S, HUANG Y, ZHU N, ZHAO Y. The rice WUSCHEL- related homeobox genes are involved in reproductive organ development, hormone signaling and abiotic stress response. Gene, 2014, 549(2): 266-274.
doi: 10.1016/j.gene.2014.08.003
[17] MARCHLER-BAUER A, BO Y, HAN L Y, HE J, LANCZYCKI C J, LU S, CHITSAZ F, DERBYSHIRE M K, GEER R C, GONZALES N R, GWADZ M, HURWITZ D I, LU F, MARCHLER G H, SONG J S, THANKI N, WANG Z X, YAMASHITA R A, ZHANG D C, ZHENG C J, GEER L Y, BRYANT S H. CDD/SPARCLE: Functional classification of proteins via subfamily domain architectures. Nucleic Acids Research, 2017, 45(D1): 200-203.
[18] CHEN C J, Chen H, ZHANG Y, THOMAS H R, FRANK M H, HE Y H, XIA R. TBtools: An integrative toolkit developed for interactive analyses of big biological data. Molecular Plant, 2020, 13(8): 1194-1202.
doi: 10.1016/j.molp.2020.06.009
[19] CHENNE R, SUGAWARA H, KOIKE T, LOPEZ R, GIBSON T J, HIGGINS D G, THOMPSON J D. Multiple sequence alignment with the clustal series of programs. Nucleic Acids Research, 2003, 31(13): 3497-3500.
doi: 10.1093/nar/gkg500
[20] KUMAR S, STECHER G, LI M, KNYAZ C, TAMURA K. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 2018, 35(6): 1547-1549.
doi: 10.1093/molbev/msy096
[21] BAILEY T L, BODEN M, BUSKE F A, FRITH M, GRANT C E, CLEMENTI L, REN J Y, LI W, NOBLE W S. MEME SUITE: Tools for motif discovery and searching. Nucleic Acids Research, 2009, 37(suppl 2): 202-208.
[22] LESCOT M, DEHAIS P, THIJS G, MARCHAL K, MOREAU Y, VAN D P Y, ROUZE P, ROMBAUTS S. PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Research, 2002, 30(1): 325-327.
doi: 10.1093/nar/30.1.325
[23] ZHANG L J, LI X X, MA B, GAO Q, DU H L, HAN Y H, LI Y, GAO Y H, QI M, ZHU Y X, LU H W, MA M C, LIU L L, ZHOU J P, NAN C H, QIN Y J, WANG J, GUI L, LIU H M, LIANG C Z, QIAO Z J. The tartary buckwheat genome provides insights into rutin biosynthesis and abiotic stress tolerance. Molecular Plant, 2017, 10(9): 1224-1237.
doi: 10.1016/j.molp.2017.08.013
[24] MI Y L, ZHU Z H, QIAN G T, LI Y, MENG X X, XUE J P, CHEN Q F, SUN W, SHI Y H. Inducing hairy roots by Agrobacterium rhizogenes-mediated transformation in Tartary buckwheat (Fagopyrum tataricum). Journal of Visualized Experiments, 2020(157): e60828.
[25] HOU S Y, SUN Z X, LINGHU N, WANG Y G, HUANG K S, XU D M, HAN Y H. Regeneration of buckwheat plantlets from hypocotyl and the influence of exogenous hormones on rutin content and rutin biosynthetic gene expression in vitro. Plant Cell, Tissue and Organ Culture, 2015, 120(3): 1159-1167.
doi: 10.1007/s11240-014-0671-5
[26] MAYER K F, SCHOOF H, HAECKER A, LENHARD M, JURGENS G, LAUX T. Role of WUSCHEL in regulating stem cell fate in the Arabidopsis shoot meristem. Cell, 1998, 95(6): 805-815.
doi: 10.1016/S0092-8674(00)81703-1
[27] WU C C, LI F W, KRAMER E M. Large-scale phylogenomic analysis suggests three ancient superclades of the WUSCHEL-related homeobox transcription factor family in plants. PLoS ONE, 2019, 14(10): e0223521.
doi: 10.1371/journal.pone.0223521
[28] HAO Q, ZHANG L, YANG Y, SHAN Z, ZHOU X A. Genome wide analysis of the WOX gene family and function exploration of GmWOX18 in soybean. Plants (Basel), 2019, 8(7): 215.
[29] VENKATA B, SCHRICK K. START Domains in Lipid/Sterol Transfer and Signaling in Plants. Michigan State University: Michigan State University Press, 2006.
[30] MUKHERJEE K, BURGLIN T R. MEKHLA, a novel domain with similarity to PAS domains, is fused to plant homeodomain-leucine zipper III proteins. Plant Physiology, 2006, 140(4): 1142-1150.
doi: 10.1104/pp.105.073833
[31] ZHANG X, ZONG J, LIU J H, YIN J Y, ZHANG D B. Genome-wide analysis of WOX gene family in rice, sorghum, maize, Arabidopsis and poplar. Journal of Integrative Plant Biology, 2010, 52(11): 1016-1026.
doi: 10.1111/jipb.2010.52.issue-11
[32] HOQUE M E, MANSFIELD J W. Effect of genotype and explant age on callus induction and subsequent plant regeneration from root derived callus of indica rice genotypes. Plant Cell Tissue and Organ Culture, 2004, 78(3): 217-223.
doi: 10.1023/B:TICU.0000025640.75168.2d
[33] 张小红, 闵东红, 邵景侠. 小麦愈伤组织诱导及原生质体的分离与纯化. 中国农学通报, 2010, 26(21): 49-53.
ZHANG X H, MIN D H, SHAO J X. Wheat callus induction and protoplasts of separation and purification. China Agricultural Journal, 2010, 26(21): 49-53. (in Chinese)
[34] 王鹏姬. 荞麦愈伤组织培养及其黄酮合成研究[D]. 杨凌: 西北农林科技大学, 2013.
WANG P J. Research on callus culture and flavonoids biosynthesis of buckwheat[D]. Yangling: Northwest A&F University, 2013. (in Chinese)
[35] LIU B L, WANG L, ZHANG J, LI J B, ZHENG H Q, CHEN J, LU M Z. WUSCHEL-related homeobox genes in Populus tomentosa: Diversified expression patterns and a functional similarity in adventitious root formation. BMC Genomics, 2014, 15: 296.
doi: 10.1186/1471-2164-15-296
[36] GUO F, ZHANG H, LIU W, HU X, HAN N, QIAN Q, XU L, BIAN H. Callus initiation from root explants employs different strategies in rice and Arabidopsis. Plant Cell Physiology, 2018, 59(9): 1782-1789.
doi: 10.1093/pcp/pcy095
[37] DEVEAUX Y, TOFFANO-NIOCHE C, CLAISSE G, THAREAU V, MORIN H, LAUFS P, MOREAU H, KREIS M, LECHARNY A. Genes of the most conserved WOX clade in plants affect root and flower development in Arabidopsis. BMC Evolutionary Biology, 2008, 8: 291.
doi: 10.1186/1471-2148-8-291
[38] HIRAKAWA Y, KONDO Y, FUKUDA H. TDIF peptide signaling regulates vascular stem cell proliferation via the WOX4 homeobox gene in Arabidopsis. The Plant Cell, 2010, 22(8): 2618-2629.
doi: 10.1105/tpc.110.076083
[39] ETCHELLS J P, PROVOST C M, MISHRA L, TURNER S. WOX4 and WOX14 act downstream of the PXY receptor kinase to regulate plant vascular proliferation independently of any role in vascular organization. Development, 2013, 140: 2224-2234.
doi: 10.1242/dev.091314
[1] WANG JiaNuo, CHEN GuiPing, LI Pan, WANG LiPing, NAN YunYou, HE Wei, FAN ZhiLong, HU FaLong, CHAI Qiang, YIN Wen, ZHAO LiaoHao. Photo-Physiological Mechanism at Grain Filling Stage of No-Tillage with Plastic Re-Mulching to Increase Maize Yield in Oasis Irrigation Areas [J]. Scientia Agricultura Sinica, 2026, 59(6): 1189-1202.
[2] CUI ShiYou, CHEN PengJun, MIAO YuanQing, HAN JiJun, SHEN JunMing. Development and Field Evaluation of Glyphosate-Resistant Wheat Germplasm Generated Through EMS Mutagenesis [J]. Scientia Agricultura Sinica, 2026, 59(4): 723-733.
[3] LIAO TingLu, SHI YaFei, XIAO DongHao, SHE YangMengFei, GUO FuCheng, YANG JiuJu, TANG HaiJiang, LUO ChengKe. The Effect of Exogenous Nitroprusside on Sugar Metabolism in Rice Seedlings Under Alkaline Stress [J]. Scientia Agricultura Sinica, 2026, 59(2): 265-277.
[4] ZHANG TianYu, LI Bai, ZANG JinPing, CAO HongZhe, DONG JinGao, XING JiHong, ZHANG Kang. Genome-Wide Identification and Expression Analysis of HMG Family Genes in Botrytis cinerea [J]. Scientia Agricultura Sinica, 2025, 58(4): 704-718.
[5] ZHANG LinLin, GONG Rui, CUI YanLing, ZHONG XiongHui, LI Ye, LI RanHong, QIAN ZongWei. Effect Analysis of SmWRKY30 in Eggplant Resistance to Ralstonia solanacearum by Virus Induced Gene Silencing (VIGS) [J]. Scientia Agricultura Sinica, 2025, 58(3): 548-563.
[6] ZHANG XiangKun, LI JiaYing, QIAO RuMeng, HE JingLei, WANG Li, SHI XiaoXin, DU GuoQiang. Effects of GFabV Under Different Zn Levels on Photosynthetic Efficiency and Photosynthesis-Related Gene Expression of ‘Shine Muscat’ Grapevine [J]. Scientia Agricultura Sinica, 2025, 58(24): 5190-5200.
[7] DING Ning, QI EnFang, JIA XiaoXia, HUANG Wei, MA LiRong, LI JianWu, YAN RuNan. Screening and Identification of miRNAs in Potato Seedlings in Response to High Temperature Stress [J]. Scientia Agricultura Sinica, 2025, 58(22): 4589-4602.
[8] ZHANG Jie, HU ChenXi, QI JianBo, ZHANG YongTai, CHEN YiBo, ZHANG YongJi. Effects of Exogenous Zeatin on Photosynthetic Parameter, Antioxidant System and Expression of Genes Related to Zeatin Synthesis in Pepper Under Low-Temperature Combined with Low-Light Stress [J]. Scientia Agricultura Sinica, 2025, 58(19): 3959-3969.
[9] CHEN BingXian, ZHANG Qi, DAI ZhangYan, ZHOU Xu, LIU Jun. Physiological and Molecular Effects of Salicylic Acid on Rice Seed Germination at Low Temperature [J]. Scientia Agricultura Sinica, 2024, 57(7): 1220-1236.
[10] WANG ChengZe, ZHANG Yan, FU Wei, JIA JingZhe, DONG JinGao, SHEN Shen, HAO ZhiMin. Bioinformatics and Expression Pattern Analysis of Maize ACO Gene Family [J]. Scientia Agricultura Sinica, 2024, 57(7): 1308-1318.
[11] SU XiaoYu, TAN ZhengWei, LI ChunMing, LI Lei, LU DanDan, YU YongLiang, DONG Wei, AN SuFang, YANG Qing, SUN Yao, XU LanJie, YANG HongQi, LIANG HuiZhen. Analysis of Genome-Wide Methylation Differences and Associated Gene Expression of Sesame Varieties Under High Temperature Stress [J]. Scientia Agricultura Sinica, 2024, 57(24): 4825-4838.
[12] YANG HaoRong, JIA Fan, HU Xu, MU Rong, LIU WeiNa, LIU ChangYun, WANG ShanZhi, SUN XianChao, MA GuanHua, CHEN GuoKang. BnJAZ7 Promotes Sclerotinia sclerotiorum Infection by Affecting the Antioxidant Pathway in Brassica napus [J]. Scientia Agricultura Sinica, 2024, 57(19): 3799-3809.
[13] LIU Tong, WANG ZhiRong, LI Wei, LIU Yang, WANG XiangRu, LAI DiLi, HE YuQi, ZHANG KaiXuan, ZHAO ZhenJun, ZHOU MeiLiang. Function Analysis of bHLH93 Transcription Factor in Tartary Buckwheat in Response to Aluminum Stress [J]. Scientia Agricultura Sinica, 2024, 57(16): 3127-3141.
[14] HU DanDan, LUO RunQi, LIANG RuiYing, WANG Lei, LIANG Lin, SI HongBin, DING JiaBo, TANG XinMing. Research Progress of ApiAP2 Transcription Factors in Regulating the Growth and Development of Toxoplasma gondii [J]. Scientia Agricultura Sinica, 2024, 57(13): 2687-2697.
[15] WEI XiaoDong, SONG XueMei, WANG Ning, ZHAO QingYong, ZHU Zhen, CHEN Tao, ZHAO Ling, WANG CaiLin, ZHANG YaDong. Distribution Characteristics of Photosynthetic Products of Nanjing Series of Super Rice During Filling Stage [J]. Scientia Agricultura Sinica, 2024, 57(12): 2309-2321.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd