葡萄全基因组DELLA蛋白基因家族鉴定及其应答外源赤霉素调控葡萄果实发育的特征

doi:10.3864/j.issn.0578-1752.2018.16.009

Abstract

Abstract: 【Objective】The objectives of this study are to identify the DELLA protein family genes from grapevine (Vitis vinifera) genome, to know the profile of DELLA protein family such as gene number, gene structure and tissue expression in grape, and to explore the mechanism of DELLA protein in gibberellic acid (GA) signaling pathway and in seedless fruit development of grapevine.【Method】DELLA protein genes in grape genome were identified by HMMER and NCBI CDD software based on DELLAgenes from Arabidopsis and rice. The full-length cDNAs were obtained by clone techniques from grapevine cv. ‘Rosario Bianco’. Cis-elements of their promoters were identified to predict their potential functions. Their chromosomal localization, gene structures, phylogenetic analysis, physicochemical properties, subcellular localizations and protein interactions were analyzed by bioinformatics analysis softwares. Subcellular localization of DELLA proteins were observed by Agrobacterium-mediated transient expression in tobacco leaf. The qRT-PCR method was used to detect the temporal and spatial expression of DELLA protein genes in the grape berry pericarp, berry flesh and seed (or seed area) induced by exogenous GA.【Result】A total of 3 DELLA proteingenes were identified from grape genome, their precise sequences were cloned and verified, named VvGAI1 (VIT_201s0011g05260), VvRGA (VIT_214s0006g00640) and VvSLR1 (VIT_211s0016g04630), respectively. Their chromosomal localization, open reading frame (ORF), number of amino acid of DELLA genes were Chr1, 1 773 bp, 590; Chr14, 1 710 bp, 569; Chr11, 1 599 bp, 532, respectively. Gene structure analysis result showed that there no intron and only one exon, and gene structures were highly conserved. Phylogenetic analysis showed that VvGAI1 was closely related to VvRGA, and were clustered into one group, with VvSLR1 was another group. The promoters of the 3 genes all contained elements that were responsible for GA and endosperm development, suggesting that they might be involved in response to GA signaling and endosperm development. Subcellular localization result showed that all 3 grape DELLA proteins were located in the nucleus. The results of qRT-PCR showed that the expression of 3 DELLA genes, except for VvSLR1 in the berry pericarp had a peak of expression at near maturity, the rest were highly expressed in young fruit stage, and exogenous GA treatment all reduced the expression of three DELLA protein genes in the grape berry pericarp, berry flesh and seed area, especially in the seed area. Protein interaction analysis showed that all 3 DELLA proteins were the core components of GA signal transduction in grape, which may interact with GIDI1 and SLY1 in GA signal transduction.【Conclusion】DELLA protein gene family in grapevine contains 3 genes. Their structures among different species are highly conserved. GA may participate in the development of berry pericarp, berry flesh and seed area through negative regulation of these three members, and all 3 members may regulate the development of grapes seedless fruit by responding to GA.

Key words: grape, DELLA, subcellular location, expression, interaction, gibberellic acid (GA)

ZHANG WenYing, WANG Chen, ZHU XuDong, MA Chao, WANG WenRan, LENG XiangPeng, ZHENG Ting, FANG JingGui. Genome-Wide Identification and Expression of DELLA Protein Gene Family During the Development of Grape Berry Induced by Exogenous GA[J].Scientia Agricultura Sinica, 2018, 51(16): 3130-3146.

References

[1] LIU B, De STORME N, GEELEN D. Gibberellin induces diploid pollen formation by interfering with meiotic cytokinesis. Plant Physiology, 2017, 173(1): 338-353.

[2] LINN T Z, BACHIR D G, CHEN L, HU Y G. Effects of gibberellic acid responsive dwarfing gene Rht9 on plant height and agronomic traits in common wheat. American Journal of Agriculture and Forestry, 2017, 5(4): 102-111.

[3] KANNO Y, OIKAWA T, CHIBA Y, ISHIMARU Y, SHIMIZU T, SANO N, KOSHIBA T, KAMIYA Y, UEDA M, SEO M. AtSWEET13 and AtSWEET14 regulate gibberellin-mediated physiological processes. Nature Communications, 2016, 7: 13245.

[4] ZHAO B, LI H T, LI J J, WANG B, DAI C, WANG J, LIU K D. Brassica napus DS-3, encoding a DELLA protein, negatively regulates stem elongation through gibberellin signaling pathway. Theoretical and Applied Genetics, 2017, 130(4): 727-741.

[5] LI W J, Zhang J X, Sun H Y, WANG S M, CHEN K Q, LIU Y X, LI H, MA Y, ZHANG Z H. FveRGA1, encoding a DELLA protein, negatively regulates runner production in Fragaria vesca. Planta, 2018, 247(4): 941-951.

[6] TYLER L, THOMAS S G, HU J H, DILL A, ALONSI JM, ECKER J R, SUN T P. DELLA proteins and gibberellin-regulated seed germination and floral development in Arabidopsis. Plant Physiology, 2004, 135(2): 1008-1019.

[7] SATO T, MIYANOIRI Y, TAKEDA M, NAOE Y, MITANI R, HIRANO K, TAKEHARA S, KAINOSHO M, MATSUOKA M, TANAKA M U, KATO H. Expression and purification of a GRAS domain of SLR1, the rice DELLA protein. Protein Expression and Purification, 2014, 95(3): 248-258.

[8] VAN DE VELDE K, CHANDLER P M, VAN DER S D, ROHDE A. Differential coupling of gibberellin responses by Rht-B1c suppressor alleles and Rht-B1b in wheat highlights a unique role for the DELLA N-terminus in dormancy. Journal of Experimental Botany, 2017, 68(3): 443-455.

[9] WANG X C, ZHAO M Z, WU W M, KORIR N K, QIAN Y M, WANG Z W. Comparative transcriptome analysis of berry-sizing effects of gibberellin (GA₃) on seedless Vitis vinifera L. Genes and Genomics, 2017, 39(5): 493-507.

[10] ACHEAMPONG A K, ZHENG C L, HALALY T, GIACOMELLI L, TAKEBAYASHI Y, JIKUMARU Y, KAMIYA Y, LICHTER A, OR E. Abnormal endogenous repression of GA signaling in a seedless table grape cultivar with high berry growth response to GA application. Frontiers in Plant Science, 2017, 8: 850.

[11] DAI C, XUE H W. Rice early flowering1, a CKI, phosphorylates DELLA protein SLR1 to negatively regulate gibberellin signalling. The Embo Journal, 2010, 29(11): 1916-1927.

[12] VAN DE V K, RUELENS P, GEUTEN K, ROHDE A, VAN DER S D. Exploiting DELLA signaling in cereals. Trends in Plant Science, 2017, 22(10): 880-893.

[13] ITOH H, Ueguchi-TANAKA M, SATO Y, ASHIKARI M, MATSUOKA M. The gibberellin signaling pathway is regulated by the appearance and disappearance of SLENDER RICE1 in nuclei. The Plant Cell, 2002, 14(1): 57-70.

[14] GAO Y, CHEN J M, ZHAO Y, LI T T, WANG M L. Molecular cloning and expression analysis of a RGA-like gene responsive to plant hormones in Brassica napus. Molecular Biology Reports, 2012, 39(2): 1957-1962.

[15] DE LUCAS M, DAVIERE J M, RODRIGUEZ-FALCON M, PONTIN M, IGLESIAS-PEDRAZ J M, LORRAIN S, FANKHAUSER C, BLAZQUEZ M A, TIARENKO E, PRAT S. A molecular framework for light and gibberellin control of cell elongation. Nature, 2008, 451: 480-484.

[16] FU X D, RICHARDS D E, AIT-ALI T, Hynes L W, Ougham H, Peng J, Harberd N P. Gibberellin-mediated proteasome- dependent degradation of the barley DELLA protein SLN1 repressor. The Plant Cell, 2002, 14(12): 3191-3200.

[17] 吴建明, 黄杏, 丘立杭, 陈荣发, 李杨瑞, 杨柳, 苏天明. DELLA蛋白在植物中的研究进展. 农业生物技术学报, 2016, 24(8): 1207-1215.

WU J M, HUANG X, QIU L H, CHEN R F, LI Y R, YANG L, SU T M. Progress on DELLA protein in plant. Journal of Agricultural Biotechnology, 2016, 24(8): 1207-1215. (in Chinese)

[18] 杨光, 曹雪, 房经贵, 宋长年, 王晨, 王西成. ‘藤稔’葡萄VvGAI基因的克隆、亚细胞定位及时空表达分析. 园艺学报, 2011, 38(10): 1883-1892.

YANG G, CAO X, FANG J G, SONG C N, WANG C, WANG X C. Cloning, subcellular localization and spatiotemporal expression of a VvGAI gene from grapevine ‘Fujiminori’. Acta Horticulturae Sinica,2011, 38(10): 1883-1892. (in Chinese)

[19] 任国慧, 上官凌飞, 房经贵, 慕茜, 冷翔鹏, 孙欣. 葡萄DELLA家族成员VvRGA和VvRGL1的预测、验证及生物信息学分析. 南京农业大学学报, 2013, 36(3): 15-21.

REN G H, SHNAGGUAN L F, FANG J G, MU Q, LENG X P, SUN X. The prediction, validation and bioinformatics analysis of the grape DELLA family members about VvRGA and VvRGL1. Journal of Nanjing Agricultural University, 2013, 36(3): 15-21. (in Chinese)

[20] 张彦苹, 王晨, 于华平, 蔡斌华, 房经贵. 适于葡萄不同组织RNA提取方法的筛选. 西北农业学报, 2010, 19(11): 135-140.

ZHNAG Y P, WANG C, YU H P, CAI B H, FANG J G. Screening of RNA extraction methods for various grapevine organs and tissues. Acta Agriculturae Boreali-occidentalis Sinica, 2010, 19(11): 135-140. (in Chinese)

[21] FAN S, ZHANG D, XING L B, QI S Y, DU L S, WU H Q, SHAO H X, LI Y M, MA J J, HAN M Y. Phylogenetic analysis of IDD gene family and characterization of its expression in response to flower induction in Malus. Molecular Genetics and Genomics, 2017, 292(4): 755-771.

[22] 杨占武, 杨君, 张艳, 吴金华, 李志坤, 王省芬, 吴立强, 张桂寅, 马峙英. 棉花GbRvd的亚细胞定位及其超表达烟草抗病性分析. 中国农业科学, 2016, 49(21): 4065-4073.

YANG Z W, YANG J, ZHANG Y, WU J H, LI Z K, WANG X F, WU L Q, ZHANG G Y, MA Z Y. Subcellular localization and verticillium wilt resistance analysis of cotton GbRvd in overexpressed tobacco. Scientia Agricultura Sinica, 2016, 49(21): 4065-4073. (in Chinese)

[23] CHENG C X, JIAO C, SINGER S D, GAO M, XU X Z, ZHOU Y M, LI Z, FEI Z J, WANG Y J, WANG X P. Gibberellin-induced changes in the transcriptome of grapevine (Vitis labrusca× V. vinifera) cv. Kyoho flowers. BMC Genomics, 2015, 16: 128.

[24] CHENG C X, XU X Z, SINGER S D, LI J, ZHANG H J, GAO M, WANG L, SONG J Y, WANG X P. Effect of GA₃ treatment on seed development and seed-related gene expression in grape. PLoS One, 2013, 8(11): e80044.

[25] WANG M Q, SUN X, WANG C, CUI L W, CHEN L D, ZHNAG C B, SHANGGUAN L F, FANG J G. Characterization of miR061 and its target genes in grapevine responding to exogenous gibberellic acid. Functional and Integrative Genomics, 2017, 17(5): 537-549.

[26] CAMUT L, DAVIERE J M, ACHARD P. Dynamic regulation of DELLA protein activity: SPINDLY and SECRET AGENT unmasked!. Molecular Plant, 2017, 10(6): 785-787.

[27] GALLEGO-BARTOLOME J, MINGUET E G, MARIN J A, PRAT S, BIAZQUEZ M A, ALABADI D. Transcriptional diversification and functional conservation between DELLA proteins in Arabidopsis. Molecular Biology and Evolution, 2010, 27(6): 1247-1256.

[28] AN J, HOU L, LI C, WANG C X, XIA H, ZHAO C Z, LI C S, ZHENG Y X, ZHAO Y X, WANG X J. Cloning and expression analysis of four DELLA genes in peanut. Russian Journal of Plant Physiology, 2015, 62(1): 116-126.

[29] Hu M Y, LUO M, XIAO Y H, LI X B, TAN K L, HOU L, DONG J, LI D M, SONG S Q, ZHAO J, ZANG Z L, LI B L, PEI Y. Brassinosteroids and auxin down-regulate DELLA genes in fiber initiation and elongation of cotton. Journal of Integrative Agriculture, 2011, 10(8): 1168-1176.

[30] SUN T P. Gibberellin-GID1-DELLA: a pivotal regulatory module for plant growth and development. Plant Physiology, 2010, 154(2): 567-570.

[31] SUZUKI H, PARK S H, OKUBO K, KITAMURA J, UEGUCHI- TANKA M, LUCHI S, KATOH E, KOBAYASHI M, YAMAGUCHI L, MATSUOKA M, ASAMI T, NAKAJIMA M. Differential expression and affinities of Arabidopsis gibberellin receptors can explain variation in phenotypes of multiple knock-out mutants. The Plant Journal, 2009, 60(1): 48-55.

[32] NELSON S K, STEBER C M. Transcriptional mechanisms associated with seed dormancy and dormancy loss in the gibberellin-insensitivesly1-2 mutant of Arabidopsis thaliana. PloS One, 2017, 12(6): e0179143.

[33] FENG S H, MARTINEZ C, GUSMAROLI G, WANG Y, ZHOU J L, WANG F, CHEN L Y, YU L, IGLESIAS-PEDRAZ J M, KIRCHER S, SCHAFER E, FU X D, FAN L M, DENG X W. Coordinated regulation of Arabidopsis thaliana development by light and gibberellins. Nature, 2008, 451 (7177): 475-479.

[34] ZHANG Z L, OGAWA M, FLEET C M, ZENTELLA R, HU J H, HEO J O, LIM J, KAMIYA Y, YAMAGUCHI S, SUN T P. Scarecrow-like 3 promotes gibberellin signaling by antagonizing master growth repressor DELLA in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America, 2011, 108(5): 2160-2165.

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Genome-Wide Identification and Expression of DELLA Protein Gene Family During the Development of Grape Berry Induced by Exogenous GA

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