芝麻发芽期耐盐性鉴定方法研究及耐盐候选基因的挖掘

doi:10.3864/j.issn.0578-1752.2018.12.002

Abstract

Abstract: 【Objective】In order to evaluate salt tolerance of sesame germplasms and reveal the genetic basis of salt tolerance in sesame at the germination stage, optimum NaCl concentration and best salt tolerance indexes were determined, salt-tolerant sesame genotypes as well as candidate genes were identified in this study. 【Method】 Eight sesame genotypes with different salt tolerance were germinated under different NaCl concentrations (0, 50, 100, 150, 200 and 250 mmol·L^-1 ). Seven germination indexes including germination potential, germination index, vigor index, seedling rate, radicle length, embryo length and fresh weight of seedling were measured. The optimum NaCl concentration and salt tolerance indexes of sesame were determined using variance analysis, principal component analysis, membership function and correlation analysis based on the relative values of multiple indexes. In addition, genome-wide association analysis (GWAS) of salt tolerance related trait in 71 sesame germplasms was performed to find the SNP loci and candidate genes related to salt tolerance in sesame. Gene function annotation, transcriptome analyses and real-time quantitative reverse transcriptase PCR (qRT-PCR) were used to identify important candidate genes involved in salt tolerance. 【Result】The larger standard value deviations of germination indexes were observed at 100 mmol·L^-1 NaCl concentration, implying that 100 mmol·L^-1 is the optimum NaCl concentration for salt-tolerance screening in this study. The seven indexes were highly correlated with salt tolerance at germination stage, suggesting that, these indexes could be targeted for effective screening of salt-tolerance in large sesame germplasm. The genome-wide association analysis identified 7 SNP loci peaks (LG5:688003, LG7:9582027, LG10:5274091, LG10:10788493, LG11:11924186, LG14:2128695 and LG16:3930301) significantly associated with salt tolerance and 34 functional candidate genes. The transcriptome analysis and qRT-PCR showed that 21 candidate genes respond strongly to salt stress.【Conclusion】In this study, 100 mmol·L^-1 is the optimum NaCl concentration for salt-tolerance screening and seven indexes including relative seedling rate could be targeted for effective screening of salt-tolerance in large sesame germplasm. In addition, 7 SNP loci peaks significantly associated with salt tolerance were identified and 21 candidate salt-tolerant genes were discovered.

Key words: sesame, salt tolerance, genome-wide association study, salt-tolerant genes

ZHANG YuJuan, YOU Jun, LIU AiLi, LI DongHua, YU JingYin, WANG YanYan, ZHOU Rong, GONG HuiHui, ZHANG XiuRong. Screening Method for Salt Tolerance in Sesame (Sesamum indicum L.) and Identification of Candidate Salt-tolerant Genes[J].Scientia Agricultura Sinica, 2018, 51(12): 2235-2247.

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Screening Method for Salt Tolerance in Sesame (Sesamum indicum L.) and Identification of Candidate Salt-tolerant Genes

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