Scientia Agricultura Sinica ›› 2017, Vol. 50 ›› Issue (16): 3215-3225.doi: 10.3864/j.issn.0578-1752.2017.16.016

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Polymorphisms of DGAT1 Gene and Their Association with Milk Quality Traits in Yak

GAO XiaoLi1, HU Jiang1, GUO ShuZhen2, SHI BinGang1, XIE JianPeng1, LUO YuZhu1, WANG JiQing1, MU YongJuan2   

  1. 1College of Animal Science and Technology, Gansu Agricultural University/Gansu Key Laboratory of Herbivorous Animal Biotechnology, Lanzhou 730070; 2Gannan Institute of Animal Science, Hezuo 747000, Gansu
  • Received:2017-02-16 Online:2017-08-16 Published:2017-08-16

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Abstract: 【Objective】In this study, polymorphisms of yak DGAT1(diacylglycerol acyltransferase 1) gene were investigated and their effects on some milk quality traits were estimated so as to enrich the molecular genetic data for yak. 【Method】 Mutations in intron5-exon7 and intron15-exon17 of DGAT1 gene were checked by PCR-SSCP in 4 yak populations (Gannan yak, Tianzhu white yak, Qinghai yak and Wild-blood yak), and the effects of mutations on the milk quality traits were analyzed in Gannan yak.【Result】The c.562+32_c.562+33insCCGCCC was found in intron5-exon7 of yak DGAT1 gene ,and allele M and genotype MM were the dominant allele and genotype, and each yak population was moderately or low polymorphic (PIC＜0.5). The 2 SNPs (c.1249-23C＞T and c.1336C＞T) were found in intron15-exon17, and nucleotide substitution c.1336C＞T at exon 17 resulted in p.Arg450Cys change. Allele A was the dominant allele, the genotype AA in Gannan yak, Tianzhu white yak and Qinghai yak and AB in Wild-blood yak were the dominant genotype, respectively. Each yak population was moderately polymorphic (0.25＜PIC＜0.5). Total 6 haplotypes and 11 haplotype combinations were constituted by mutations in these two regions, and there was a linkage relationship between 3 mutations and closed to linkage equilibrium. The association analysis showed that the genotype in intron5-exon7 was not significantly correlated with the milk quality traits in Gannan yak. The milk quality traits of genotype BB were the lowest, and the milk protein rate, fat rate, total solids percentage were lower than those of other genetypes (P＜0.05), and individuals with possessing allele A had higher milk protein rate, fat rate, total solids percentage and non-fat solids percentage than those no-possessing (P＜0.05), and yaks with allele C also showed higher milk fat rate than those no-possessing (P＜0.05), but individuals with allele B showed lower milk fat rate and total solids percentage than those no-possessing (P＜0.05) in intron15-exon17 of DGAT1 gene in Gannan yak. Individuals with haplotype combination H1H3 had higher milk protein rate, fat rate, total solids percentage and non-fat solids percentage, and the milk fat rate and total solids percentage were higher than those of other haplotype combinations (P＜0.05), while those with haplotype combination H2H2 were lower, in addition to the lactose rate, the other traits were lower than other combinations (P＜0.05). 【Conclusion】The intron5-exon7 and intron15-exon17 of yak DGAT1 geneare low and moderately polymorphic, and the c.562+32_c.562+33insCCGCCC in intron6 and 2 SNPs (c.1249-23C＞T, c.1336C＞T) in intron15 and exon17 were detected, respectively. The mutations in intron15-exon17 of DGAT1 gene may affect some milk quality traits of Gannan yak, and selecting yak with allele A and haplotype H1H3, or eliminating ones with allele B and haplotype H2H2 can improve the milk quality of Gannan yak.

Key words: yak, DGAT1 gene, polymorphism, milk quality traits

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