柑橘砧木对砂糖橘果实糖积累的影响

doi:10.3864/j.issn.0578-1752.2015.11.013

Abstract

Abstract: 【Objective】 The effects of different rootstocks on sugar accumulation, hormone role, sucrose-related enzyme activities and gene expression of ‘Shatangju’ tangerine fruits were studied. The aim is to make research on the physiological and molecular mechanism of citrus rootstock effects on fruit sugar accumulation, and to establish a theoretical basis for the use of rootstocks in citrus production. 【Method】 ‘Shatangju’ tangerine, grafted on Trifoliate orange and Canton lemon rootstocks, were used in this study. The sugar content and sucrose metabolism enzyme activities were determined in fruits of ‘Shatangju’ tangerine. The expression levels of related genes, including sucrose metabolism enzymes and sucrose transport proteins, were obtained from the fruits. Meanwhile, abscisic acid (ABA) levels and expression patterns of NCED1 gene were also measured in the fruits.【Result】 Results showed that total soluble sugar and sucrose content were significantly higher in both the peel and pulp of fruits on Trifoliate orange rootstocks than those on Canton lemon rootstocks at maturation stage. In the meantime, there were higher activities of sucrose synthase (SS) in the fruits on Trifoliate orange rootstocks. qRT-PCR analysis showed that the expression levels of SS2 gene were higher in the fruits on Trifoliate orange rootstocks than those on Canton lemon rootstocks. On the contrary, CWINV1 expression levels were higher in the fruits of ‘Shatangju’/Canton lemon, and was negatively related to sucrose content (r=-0.648*). Furthermore, SUC3 expression increased along with maturity and showed higher levels in the fruits of ‘Shatangju’/Trifoliate orange. There was a significantly positive correlation between SUC3 expression and total soluble sugar content (r=0.87*). In addition, the ABA concentration gradually increased in the peel during fruit ripening, and the ABA concentration was significantly and positively correlated with SS2 and SUC3 expression and significantly greater in ‘Shatangju’/Trifoliate orange after December 5. In the pulp, the ABA concentration time series followed a flattened curve, and the ABA concentration of the pulp from the ‘Shatangju’/Trifoliate orange was also greater. In the peel, NCED1 expression gradually increased with fruit ripening, and showed higher levels in ‘Shatangju’/Trifoliate orange. NCED1 gene was up-regulated and exhibited significant higher levels in the pulp of ‘Shatangju’/Trifoliate orange at later stage. 【Conclusion】 In short, the choice of rootstock had a significant effect on sugar accumulation of ‘Shatangju’ tangerine fruits, mainly through changing the activities and gene expression patterns of sucrose metabolism enzymes, sucrose transport and ABA levels in the fruits.

Key words: citrus, rootstock, sugar accumulation, sucrose metabolism enzymes, gene expression, ABA

LIU Xiang-yu, LI Juan, HUANG Min, LIANG Chun-hui, CHEN Jie-zhong. Research on Influences of Rootstock on Sugar Accumulation in ‘Shatangju’ Tangerine Fruits[J].Scientia Agricultura Sinica, 2015, 48(11): 2217-2228.

References

[1] Cantuarias-Avilés T, Mourão Filho A, Stuchi E S, Silva S D, Espinoza-Núñez E. Tree performance and fruit yield and quality of ‘Okitsu’ Satsuma mandarin grafted on 12 rootstocks. Scientia Horticulturae, 2010, 123(3): 318-322.

[2] Hussain S, Curk F, Anjum M A, Pailly O, Tison G. Performance evaluation of common Clementine on various citrus rootstocks. Scientia Horticulturae, 2013, 150: 278-282.

[3] 淳长品, 彭良志, 雷霆, 唐海涛, 曹立, 江才伦, 凌丽俐. 不同柑橘砧木对锦橙果实品质的影响. 园艺学报, 2010, 37(6): 991-996.

Chun C P, Peng L P, Lei T, Tang H T, Cao L, Jiang C L, Ling L L. Effects of rootstocks on fruit quality of ‘Jincheng’ sweet orange. Acta Horticulturae Sinica, 2010, 37(6): 991-996. (in Chinese)

[4] 郑永强, 邓烈, 何绍兰, 周志钦, 易时来, 毛莎莎, 赵旭阳.几种砧木对哈姆林甜橙植株生长、产量及果实品质的影响. 园艺学报, 2010, 37(4): 532-538.

Zheng Y Q, Deng L, He S L, Zhou Z Q, Yi S L, Mao S S, Zhao X Y. Effects of seven rootstocks on tree growth, yield and fruit quality of ‘Hamlin’ sweet orange in South China. Acta Horticulturae Sinica, 2010, 37(4): 532-538. (in Chinese)

[5] D’Aoust M A, Yelle S, Nguyen-Quoc B. Antisense inhibition of tomato fruit sucrose synthase decreases fruit setting and the sucrose unloading capacity of young fruit. The Plant Cell Online, 1999, 11(12): 2407-2418.

[6] Yu X, Wang X, Zhang W, Qian T, Tang G, Guo Y, Zheng C. Antisense suppression of an acid invertase gene (MAI1) in muskmelon alters plant growth and fruit development. Journal of Experimental Botany, 2008, 59(11): 2969-2977.

[7] Yamada K, Kojima T, Bantog N, Shimoda T, Mori H, Shiratake K, Yamaki S. Cloning of two isoforms of soluble acid invertase of Japanese pear and their expression during fruit development. Journal of Plant Physiology, 2007, 164(6): 746-755.

[8] Komatsu A, Takanokura Y, Moriguchi T, Omura M, Akihama T. Differential expression of three sucrose phosphate synthase isoforms during accumulation in citrus fruit (Citrus unshiu Mare). Plant Science, 1999, 140: 169-178.

[9] Braun D M, Wang L, Ruan Y L. Understanding and manipulating sucrose phloem loading, unloading, metabolism, and signalling to enhance crop yield and food security. Journal of Experimental Botany, 2014, 65(7): 1713-1735.

[10] 刘慧英, 朱祝军, 钱琼秋, 葛志平. 砧木对小型早熟西瓜果实糖代谢及相关酶活性的影响. 园艺学报, 2004, 31(1): 47-52.

Liu H Y, Zhu Z J, Qian Q Q, Ge Z P. The effects of different rootstocks on the sugar metabolism and related enzyme activities in small and early-maturing watermelon during fruit development. Acta Horticulturae Sinica, 2004, 31(1): 47-52. (in Chinese)

[11] 孟文慧, 张显, 罗婷. 嫁接砧木对西瓜果实糖分积累及蔗糖代谢相关酶活性的影响. 西北农林科技大学学报: 自然科学版, 2009, 37(3): 127-132.

Meng W H, Zhang X, Luo T. Influences of rootstocks on the sugar accumulation and activities of sucrose metabolism related enzymes in Citrullus lanatus by grafting. Journal of Northwest A& F University: National Science Edition, 2009, 37(3): 127-132. (in Chinese)

[12] León P, Sheen J. Sugar and hormone connections. Trends in Plant Science, 2003, 8(3): 110-116.

[13] Carrari F, Fernie A R, Iusem N D. Heard it through the grapevine? ABA and sugar cross-talk: the ASR story. Trends in Plant Science, 2004, 9(2): 57-59.

[14] Pan Q H, Li M J, Peng C C, Zhang N, Zou X, Zou K Q, Yu X C, Wang X F, Zhang D P. Abscisic acid activates acid invertases in developing grape berry. Physiologia Plantarum, 2005, 125(2): 157-170.

[15] Tang T, Xie H, Wang Y, Lu B, Liang J. The effect of sucrose and abscisic acid interaction on sucrose synthase and its relationship to grain filling of rice (Oryza sativa L.). Journal of Experimental Botany, 2009, 60(9): 2641-2652.

[16] Akihiro T, Umezawa T, Ueki C, Lobna B, Mizuno K, Ohta M, Fujimura T. Genome wide cDNA-AFLP analysis of genes rapidly induced by combined sucrose and ABA treatment in rice cultured cells. FEBS Letters, 2006, 580: 5947-5952.

[17] 杨子琴, 李茂, 章笑赟, 余意, 王惠聪, 黄旭明. 饥饿胁迫对龙眼果实脱落及糖代谢的影响. 果树学报, 2011, 28(3): 428-432.

Yang Z Q, Li M, Zhang X Y, Yu Y, Wang H C, Huang X M. Effects of starvation stress on fruit abscission and sugar metabolism in longan. Journal of Fruit Science, 2011, 28(3): 428-432. (in Chinese)

[18] 赵智中, 张上隆, 徐昌杰, 陈昆松, 刘拴桃. 蔗糖代谢相关酶在温州蜜柑果实糖积累中的作用. 园艺学报, 2001, 28(2): 112-118.

Zhao Z Z, Zhang S L, Xu C J, Chen K S, Liu S T. Roles of sucrose-metabolizing enzymes in accumulation of sugars in Satsuma mandarin fruit. Acta Horticulturae Sinica, 2001, 28(2): 112-118. (in Chinese)

[19] Moriguchi T, Abe K, Sanada T, Yamaki S. Levels and role of sucrose synthase, sucrose-phosphate synthase, and acid invertase in sucrose accumulation in fruit of Asian pear. Journal of the American Society for Horticultural Science, 1992, 117(2): 274-278.

[20] Burger Y, Schaffer A A. The contribution of sucrose metabolism enzymes to sucrose accumulation in Cucumis melo. Journal of the American Society for Horticultural Science, 2007, 132(5): 704-712.

[21] Kubo T, Hohjo I, Hiratsuka S. Sucrose accumulation and its related enzyme activities in the juice sacs of satsuma mandarin fruit from trees with different crop loads. Scientia Horticulturae, 2001, 3(4): 215-225.

[22] Wang F, Sanz A, Brenner M L, Smith A. Sucrose synthase, starch accumulation, and tomato fruit sink strength. Plant Physiology, 1993, 101(1): 321-327.

[23] 闫梅玲, 代红军, 单守明, 王振平, 范永, 周明.蔗糖代谢相关酶对果实糖积累影响的研究进展. 安徽农业科学, 2009, 37(29): 14021-14023.

Yan M L, Dai H J, Dan S M, Wang Z P, Fan Y, Zhou M. Research progress of the influence of sucrose-metabolizing enzymes on the sugar accumulation in fruit. Journal of Anhui Agricultural Sciences, 2009, 37(29): 14021-14023. (in Chinese)

[24] Hockema B R, Etxeberria E. Metabolic contributors to drought- enhanced accumulation of sugars and acids in oranges. Journal of the American Society for Horticultural, 2001, 126(5): 599-605.

[25] Miron D, Petreikov M, Carmi N, Shen S, Levin I, Granot D, Zamski E, Schaffer A A. Sucrose uptake, invertase localization and gene expression in developing fruit of Lycopersicon esculentum and the sucrose-accumulating Lycopersicon hirsutum. Physiologia Plantarum, 2002, 115(1): 35-47.

[26] Geromel C, Ferreira L P, Guerreiro S M, Cavalari A A, Pot D, Pereira L F, Leroy T, Vieira L G, Mazzafera P, Marraccini P. Biochemical and genomic analysis of sucrose metabolism during coffee (Coffea arabica) fruit development. Journal of Experimental Botany, 2006, 57(12): 3243-3258.

[27] Klann E M, Chetelat R T, Bennett A B. Expression of acid invertase gene controls sugar composition in tomato (Lycopersicon) fruit. Plant Physiology, 1993, 3(3): 863-870.

[28] Klann E M, Hall B, Bennett A B. Antisense acid invertase (TIV1) gene alters soluble sugar composition and size in transgenic tomato fruit. Plant Physiology, 1996, 12(3): 1321-1330.

[29] Ruan Y L, Jin Y, Yang Y J, Boyer J S. Sugar input, metabolism, and signaling mediated by invertase: roles in development, yield potential, and response to drought and heat. Molecular Plant, 2010, 3(6): 942-955.

[30] 袁进成, 刘颖慧. 植物糖转运蛋白研究进展. 中国农学通报, 2013, 29(36): 287-294.

Yuan J C, Liu Y H. Genetics and functional properties of sugar transporters in plants. Chinese Agricultural Science Bulletin, 2013, 29(36): 287-294. (in Chinese)

[31] Yao L C, Shi J X, Weiss D, Goldschmidt E E. Sugars regulate sucrose transporter gene expression in citrus. Biochemical and Biophysical Research Communications, 2003, 306: 402-407.

[32] Meyer S, Lauterbach C, Niedermeier M, Barth I, Sjolund R D, Sauer N. Wounding enhances expression of AtSUC3, a sucrose transporter from Arabidopsis sieve elements and sink tissues. Plant Physiology, 2004, 134(2): 684-693.

[33] Hackel A, Schauer N, Carrari F, Fernie A R, Grimm B, Kühn C. Sucrose transporter LeSUT1 and LeSUT2 inhibition affects tomato fruit development in different ways. The Plant Journal, 2006, 45: 180-192.

[34] Gambetta G A, Matthews M A, Shaghasi T H, McElrone A J, Castellarin S D. Sugar and abscisic acid signaling orthologs are activated at the onset of ripening in grape. Planta, 2010, 232(1): 219-234.

[35] 夏国海, 张大鹏, 贾文锁. IAA、GA和ABA对葡萄果实¹⁴C蔗糖输入与代谢的调控. 园艺学报, 2000, 27(1): 6-10.

Xia G H, Zhang D P, Jia W S. Effects of IAA, GA and ABA on ¹⁴C-sucrose import and metabolism in grape berries. Acta Horticulturae Sinica, 2000, 27(1): 6-10. (in Chinese)

[36] Jia H F, Chai Y M, Li C L, Lu D, Luo J J, Qin L, Shen Y Y. Abscisic acid plays an important role in the regulation of strawberry fruit ripening. Plant Physiology, 2011, 157(1): 188-199.

[37] Soto A, Ruiz K B, Ravaglia D, Costa G, Torrigiani P. ABA may promote or delay peach fruit ripening through modulation of ripening-and hormone-related gene expression depending on the developmental stage. Plant Physiology and Biochemistry, 2013, 64: 11-24.

[38] Setha S. Roles of abscisic acid in fruit ripening. Walailak Journal of Science and Technology (WJST), 2012, 9(4): 297-308.

[39] Yadava U L, Dayton D F. The relation of endogenous abscisic acid to the dwarfing capability of East Malling apple rootstocks. Journal of the American Society for Horticultural Science, 1972, 97: 701-705.

[40] Kamboj J S, Browning G, Blake P S, Quinlan J D, Baker D A. GC-MS-SIM analysis of abscisic acid and indole-3-acetic acid in shoot bark of apple rootstocks. Plant Growth Regulation, 1999, 28(1): 21-27.

[41] 姜小文, 曾继吾, 姜波, 易干军, 石雪晖. 两种砧木对年橘果实品质与产量的影响. 园艺学报, 2012, 39(2): 349-354.

Jiang X W, Zeng J W, Jiang B, Yi G J, Shi X H. The effect of rootstocks on change of fruit quality and storage on tree of citrus reticulate ‘Nianju’. Acta Horticulturae Sinica, 2012, 39(2): 349-354. (in Chinese)

[42] 赵智中, 张上隆, 陈俊伟, 陶俊, 吴延军. 柑橘品种间糖积累差异的生理基础. 中国农业科学, 2002, 35(5): 541-545.

Zhao Z Z, Zhang S L, Chen J W, Tao J, Wu Y J. The physiological mechanism on the difference of sugar accumulation in citrus varieties. Scientia Agricultura Sinica, 2002, 35(5): 541-545. (in Chinese)

[43] Zhang L Y, Peng Y B, Pelleschi Travier S. Evidence for apoplasmic phloem unloading in developing apple fruit. Plant Physiology, 2004, 135(1): 574-586.

[44] Pan Q H, Li M J, Peng C C, Zhang N, Zou X, Zou K Q, Wang X L, Yu X C, Wang X F, Zhang D P. Abscisic acid activates acid invertases in developing grape berry. Physiologia Plantarum, 2005, 125(2): 157-170.

Related Articles 15

[1]	ZHANG ZhiLin, LIU Rong, ZONG XuXiao, HAO XiaoPeng, YANG Tao. Integrated Multi-Stage Evaluation of Salt Tolerance in Vicia faba L. and Itaconic Acid-Mediated Alleviation of Germination-Stage Salt Stress [J]. Scientia Agricultura Sinica, 2026, 59(6): 1172-1188.
[2]	WANG JiaNuo, CHEN GuiPing, LI Pan, WANG LiPing, NAN YunYou, HE Wei, FAN ZhiLong, HU FaLong, CHAI Qiang, YIN Wen, ZHAO LiaoHao. Photo-Physiological Mechanism at Grain Filling Stage of No-Tillage with Plastic Re-Mulching to Increase Maize Yield in Oasis Irrigation Areas [J]. Scientia Agricultura Sinica, 2026, 59(6): 1189-1202.
[3]	CUI ShiYou, CHEN PengJun, MIAO YuanQing, HAN JiJun, SHEN JunMing. Development and Field Evaluation of Glyphosate-Resistant Wheat Germplasm Generated Through EMS Mutagenesis [J]. Scientia Agricultura Sinica, 2026, 59(4): 723-733.
[4]	JIANG Feng, WU ChunYan, WANG YiHao, YANG ZeZhong, GONG Cheng, LUO Chen. Identification and Expression Analysis of the Fatty Acid Elongase Gene Family in Bemisia tabaci MED [J]. Scientia Agricultura Sinica, 2026, 59(4): 793-806.
[5]	HAO Kun, CHEN HongDe, ZHANG Wei, ZHONG Yun, DANG MeiRong, ZHU ShiJiang, HUANG ZhiKun, JIN Ying. Comprehensive Evaluation of Water-Nitrogen Management Under Surge-Root Irrigation Based on Citrus Yield, Quality, and Water- Nitrogen Use Efficiency [J]. Scientia Agricultura Sinica, 2026, 59(4): 862-873.
[6]	LIAO TingLu, SHI YaFei, XIAO DongHao, SHE YangMengFei, GUO FuCheng, YANG JiuJu, TANG HaiJiang, LUO ChengKe. The Effect of Exogenous Nitroprusside on Sugar Metabolism in Rice Seedlings Under Alkaline Stress [J]. Scientia Agricultura Sinica, 2026, 59(2): 265-277.
[7]	LIU Jie, HOU Rui, ZHOU ZeHua, YI TuYong. Antibacterial Activity of Polyhexamethylene Guanidine Against Xanthomonas citri pv. citri [J]. Scientia Agricultura Sinica, 2025, 58(9): 1779-1790.
[8]	ZHANG TianYu, LI Bai, ZANG JinPing, CAO HongZhe, DONG JinGao, XING JiHong, ZHANG Kang. Genome-Wide Identification and Expression Analysis of HMG Family Genes in Botrytis cinerea [J]. Scientia Agricultura Sinica, 2025, 58(4): 704-718.
[9]	YAO Li, DENG ChunXiu, TANG Biao, WANG Hong, WU YueYing, ZHANG Qi, LI YuanHong, LIU ZhongYou, LU ChangAi, LIN ChaoWen. Suitability Evaluation of Late Maturing Citrus in Dongpo District, Meishan City, Sichuan Province Based on Maximum Entropy Model (MaxEnt) and Geographic Information System (GIS) [J]. Scientia Agricultura Sinica, 2025, 58(4): 748-758.
[10]	ZHANG LinLin, GONG Rui, CUI YanLing, ZHONG XiongHui, LI Ye, LI RanHong, QIAN ZongWei. Effect Analysis of SmWRKY30 in Eggplant Resistance to Ralstonia solanacearum by Virus Induced Gene Silencing (VIGS) [J]. Scientia Agricultura Sinica, 2025, 58(3): 548-563.
[11]	ZHANG XiangKun, LI JiaYing, QIAO RuMeng, HE JingLei, WANG Li, SHI XiaoXin, DU GuoQiang. Effects of GFabV Under Different Zn Levels on Photosynthetic Efficiency and Photosynthesis-Related Gene Expression of ‘Shine Muscat’ Grapevine [J]. Scientia Agricultura Sinica, 2025, 58(24): 5190-5200.
[12]	QIN Lu, SHEN DanDan, JIANG XiaoLi, XIE HePing, AO YiJun, YANG Yang, ZHU Feng, XU RangWei, LIAO WenYue, CHENG YunJiang. Effect of Water Status on the Storability of Citrus Fruits Harvested Under Continuous Rainy Weather [J]. Scientia Agricultura Sinica, 2025, 58(24): 5259-5273.
[13]	DING Ning, QI EnFang, JIA XiaoXia, HUANG Wei, MA LiRong, LI JianWu, YAN RuNan. Screening and Identification of miRNAs in Potato Seedlings in Response to High Temperature Stress [J]. Scientia Agricultura Sinica, 2025, 58(22): 4589-4602.
[14]	WANG Fan, LIU ChenWei, LU HongChen, XU RenChao, BIAN XiaoChun. Transcriptome Analysis of Vicia faba Response to Alternaria alternata Infection and Validation of the Disease Resistance Function of VfPR4 [J]. Scientia Agricultura Sinica, 2025, 58(22): 4656-4672.
[15]	LUO Qin, CHEN XieYong, XU YuYing, WEI Hang, HUANG Biao, YAO QingHua, YE NaiXing, ZHENG DeYong, YAN MingJuan. Characterization of Non-Volatile Metabolites of White Peony Tea Make of Camellia sinensis Fu’an-dabaicha from Different Origins [J]. Scientia Agricultura Sinica, 2025, 58(22): 4757-4770.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Research on Influences of Rootstock on Sugar Accumulation in ‘Shatangju’ Tangerine Fruits

RichHTML

PDF