柑橘砧木对砂糖橘果实糖积累的影响

doi:10.3864/j.issn.0578-1752.2015.11.013

Abstract

Abstract: 【Objective】 The effects of different rootstocks on sugar accumulation, hormone role, sucrose-related enzyme activities and gene expression of ‘Shatangju’ tangerine fruits were studied. The aim is to make research on the physiological and molecular mechanism of citrus rootstock effects on fruit sugar accumulation, and to establish a theoretical basis for the use of rootstocks in citrus production. 【Method】 ‘Shatangju’ tangerine, grafted on Trifoliate orange and Canton lemon rootstocks, were used in this study. The sugar content and sucrose metabolism enzyme activities were determined in fruits of ‘Shatangju’ tangerine. The expression levels of related genes, including sucrose metabolism enzymes and sucrose transport proteins, were obtained from the fruits. Meanwhile, abscisic acid (ABA) levels and expression patterns of NCED1 gene were also measured in the fruits.【Result】 Results showed that total soluble sugar and sucrose content were significantly higher in both the peel and pulp of fruits on Trifoliate orange rootstocks than those on Canton lemon rootstocks at maturation stage. In the meantime, there were higher activities of sucrose synthase (SS) in the fruits on Trifoliate orange rootstocks. qRT-PCR analysis showed that the expression levels of SS2 gene were higher in the fruits on Trifoliate orange rootstocks than those on Canton lemon rootstocks. On the contrary, CWINV1 expression levels were higher in the fruits of ‘Shatangju’/Canton lemon, and was negatively related to sucrose content (r=-0.648*). Furthermore, SUC3 expression increased along with maturity and showed higher levels in the fruits of ‘Shatangju’/Trifoliate orange. There was a significantly positive correlation between SUC3 expression and total soluble sugar content (r=0.87*). In addition, the ABA concentration gradually increased in the peel during fruit ripening, and the ABA concentration was significantly and positively correlated with SS2 and SUC3 expression and significantly greater in ‘Shatangju’/Trifoliate orange after December 5. In the pulp, the ABA concentration time series followed a flattened curve, and the ABA concentration of the pulp from the ‘Shatangju’/Trifoliate orange was also greater. In the peel, NCED1 expression gradually increased with fruit ripening, and showed higher levels in ‘Shatangju’/Trifoliate orange. NCED1 gene was up-regulated and exhibited significant higher levels in the pulp of ‘Shatangju’/Trifoliate orange at later stage. 【Conclusion】 In short, the choice of rootstock had a significant effect on sugar accumulation of ‘Shatangju’ tangerine fruits, mainly through changing the activities and gene expression patterns of sucrose metabolism enzymes, sucrose transport and ABA levels in the fruits.

Key words: citrus, rootstock, sugar accumulation, sucrose metabolism enzymes, gene expression, ABA

LIU Xiang-yu, LI Juan, HUANG Min, LIANG Chun-hui, CHEN Jie-zhong. Research on Influences of Rootstock on Sugar Accumulation in ‘Shatangju’ Tangerine Fruits[J].Scientia Agricultura Sinica, 2015, 48(11): 2217-2228.

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Research on Influences of Rootstock on Sugar Accumulation in ‘Shatangju’ Tangerine Fruits

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