Scientia Agricultura Sinica ›› 2015, Vol. 48 ›› Issue (9): 1818-1824.doi: 10.3864/j.issn.0578-1752.2015.09.15

• ANIMAL SCIENCE·VETERINARY SCIENCERE • Previous Articles     Next Articles

POMC Expression and Localization in Sheep Skin with Different Coat Color

MA Shu-hui1, LI Ya-nan1, HE Xiao-yan1, WANG Hai-dong1, YANG Lei2, DONG Chang-sheng1   

  1. 1College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, Shanxi                   
    2    College of Information, Shanxi Agricultural University, Taigu 030801, Shanxi
  • Received:2014-07-15 Online:2015-05-01 Published:2015-05-01

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Abstract: 【Objective】The objective of this study is to identify the regulation effect of POMC on coat pigment formation via analyzing the differences of POMC expression and location between different coat colors of sheep skin, in order to further enrich the theoretical knowledge about the formation mechanism of animal coat color. 【Method】 Three black adult sheep and three white sheep with matched age were selected, then three pieces of skin tissue from the buttocks of sheep were collected, respectively. One of them was used for immunohistochemistry assay to investigate the expression location of POMC between different coat color sheep after tissue embedded, the others were hold in liquid nitrogen for the extraction of total RNA and protein. The POMC-specific primers were designed by blasting in the NCBI database. After the reverse transcription of total RNA, QRT-PCR assay was proceeded to detect POMC mRNA expression of different coat color sheep. Then the protein expression level of POMC between different color sheep was evaluated by using Western blotting. All data were analyzed by SPSS19.0 to explore the mediate effect of POMC on coat color formation mechanism. 【Result】Immunohistochemistry showed that POMC was expressed in both black and white sheep skin, and the expression sites were located around the keratinocytes of hair follicle root sheath and epidermal. QRT-PCR showed that the relative expression of POMC mRNA in black sheep skin was 2.5004±0.2577**, while in white was 1.0032±0.0350, the quantity expression of POMC mRNA level of black sheep was 2.50 times as that of white sheep (P＜0.01). Western blotting result showed that the expression of POMC protein in black and white sheep were 1.5253±0.04026* and 1.0005±0.0180, respectively, the expression of POMC protein in black sheep was 1.55 times as that in white sheep (P＜0.05). 【Conclusion】POMC expressed normally in black and white sheep skin, and its expression was located in consistent position, the expression of POMC in black and white sheep skin have differences, which may involve in formation of coat color.

Key words: sheep, POMC, Western blotting, immunohistochemistry

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