Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (19): 3955-3964.doi: 10.3864/j.issn.0578-1752.2013.19.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Characterization of the Regulatory Gene hrd1(t) Involved in Anthocyanin Biosynthesis

 LIU  Yong-Zhu, LUO  Wen-Long, HUANG  Cui-Hong, CHEN  Li-Kai, WANG  Hui, CHEN  Zhi-Qiang, GUO  Tao   

  1. South China Agricultural University/National Engineering Research Center of Plant Space Breeding, Guangzhou 510642
  • Received:2013-03-25 Online:2013-10-01 Published:2013-06-17

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Abstract: 【Objective】 The objective of this study is to conduct genetic analysis and gene mapping for the purple leaf character of rice germplasm hrd1 (Oryza sativa L. ssp. indica). 【Method】 The regulatory gene hrd1, is a rice germplasm with purple leaf which originated from the hybrids of two green leaf accessions Texianzhan 13 and 02428. Major agronomic traits of hrd1 was firstly investigated, and the segregation ratio of purple and green leaf characters was assayed by using the F1, F2 and BC1F1 populations derived from the crosses of hrd1 and 02428. Fine mapping for the gene contributed to purple leaf character was further conducted by using F2 population, and the candidate gene was identified. 【Result】 The purple character initiates on leaf apex of hrd1 at the 2nd leaf stage and gradually spreads to most parts of the whole plant. At the ripening stage, the agronomic trait of hrd1 was significantly different from the control cultivar (Texianzhan 13). Chlorophyll content was equal between hrd1 and the control, while the anthocyanin content in hrd1 was much higher than that in control. A recessive gene named hrd1(t) controls the purple leaf character in hrd1 according to genetic analysis, and it was mapped to a 32.5 kb region on chromosome 4 between InDel markers HRD10 and HRD21. By sequencing for genes within the mapped region, a single base mutation (A to C) was found in the 3rd exon of LOC_Os04g50660 (encoding a WD and G-beta repeats containing protein), which leads to the predicted amino acid at position 196 converts from lysine in control to threonine in hrd1. 【Conclusion】hrd1(t) encodes a WD40-class transcription factor and may be involved in a regulatory mechanism of pigments.

Key words: rice , purple leaf trait , anthocyanin , WD40 regulatory gene

[1]程式华. 中国超级稻育种技术的创新与发展. 作物杂志, 2012, 6: 1-3.

Cheng S H. Innovation and development of techniques for super-rice breeding in China. Crops, 2012, 6: 1-3. (in Chinese)

[2]Winkel-Shirley B. Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and biotechnology. Plant Physiology, 2001, 126(2): 485-493.

[3]Winkel-Shirley B. It takes a garden. How work on diverse plant species has contributed to an understanding of flavonoid metabolism. Plant Physiology, 2001, 127(4): 1399-1404.

[4]Austin M B, Noe L J P. The chalcone synthase superfamily of type III polyketide synthases. Natural Products Report, 2003, 20: 79-110.

[5]Yamazaki M, Nakajima J, Yamanashi M, Sugiyama M, Makita Y, Springob K, Awazuhara M, Saito K. Metabolomics and differential gene expression in anthocyanin chemo-varietal forms of Perilla frutescens. Phytochemistry, 2003, 62(6): 987-995.

[6]Bogs J, Ebadi A, McDavid D, Robinson S P. Identification of the flavonold hydroxylases from grapevine and their regulation during fruit development. Plant Physiology, 2006, 140(1): 279-291.

[7]Jeong S T, Goto-Yamamoto N, Hashizume K, Esaka M. Expression of the flavonoid 3'-hydroxylase and flavonoid 3',5'-hydroxylase genes and flavonoid composition in grape (Vitis vinifera). Plant Science, 2006, 170(1): 61-69.

[8]Castellarin S D, Di Gaspero G, Marconi R, Nonis A, Peterlunger E, Paillard S, Adam-Blondon A F, Testolin R. Colour variation in red grapevines (Vitis vinifera L.): Genomic organisation, expression of flavonoid 3'-hydroxylase, flavonoid 3',5'-hydroxylase genes and related metabolite profiling of red cyanidin-/blue delphinidin-based anthocyanins in berry skin. BMC Genomics, 2006, 7: 12.

[9]Shimada S, Inoue Y T, Sakuta M. Anthocyanidin synthase in non-anthocyanin-producing Caryophyllales species. The Plant Journal, 2005, 44(6): 950-959.

[10]Nakatsuka T, Nishihara M, Mishiba K, Yamamura S. Temporal expression of flavonoid biosynthesis-related genes regulates flower pigmentation in gentian plants. Plant Science, 2005, 168(5): 1309-1318.

[11]Ramsay N A, Glover B J. MYB-bHLH-WD40 protein complex and the evolution of cellular diversity. Trends in Plant Science, 2005, 10(2): 63-70.

[12]Xie D Y, Sharma S B, Wright E, Wang Z Y, Dixon R A. Metabolic engineering of proanthocyanidins through co-expression of anthocyanidin reductase and the PAP1 MYB transcription factor. The Plant Journal, 2006, 45(6): 895-907.

[13]Reddy A R, Scheffler B, Madhuri G, Srivastava M N, Kumar A, Sathyanarayanan P V, Nair S, Mohan M. Chalcone synthase in rice (Oryza sativa L.): Detection of the CHS protein in seedlings and molecular mapping of the chs locus. Plant Molecular Biology, 1996, 32: 735-743.

[14]Druka A, Kudrna D, Rostoks N, Brueggeman R, von Wettstein D, Kleinhofs A. Chalcone isomerase gene from rice (Oryza sativa) and barley (Hordeum vulgare): Physical, genetic and mutation mapping. Gene, 2003, 302: 171-178.

[15]Reddy A M, Reddy V S, Scheffler B E, Wienand U, Reddy A R. Novel transgenic rice overexpressing anthocyanidin synthase accumulates a mixture of flavonoids leading to an increased antioxidant potential. Metabolic Engineering, 2007, 9: 95-111.

[16]Saitoh K, Onishi K, Mikami I, Thidar K, Sano Y. Allelic Diversification at the C (OsC1) locus of wild and cultivated rice:Nucleotide changes associated with phenotypes. Genetics, 2004, 168: 997-1007.

[17]Sweeney M T, Thomson M J, Pfeil B E, McCouch S. Caught red-handed: Rc encodes a basic helix-loop-helix protein conditioning red pericarp in rice. The Plant Cell, 2006, 18(2): 283-294.

[18]Kawahigashi H, Hirose S, Iwai T, Ohashi Y, Sakamoto W, Maekawa M, Ohkawa Y.  Chemically induced expression of rice OSB2 under the control of the OsPR1.1 promoter confers increased anthocyanin accumulation in transgenic rice. Journal of Agricultural and Food Chemistry, 2007, 55(4): 1241-1247.

[19]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 1980, 8(19): 4321-4325.

[20]Shen Y J, Jiang H, Jin J P, Zhang Z B, Xi B, He Y Y, Wang G, Wang C, Qian L, Li X, Yu Q B, Liu H J, Chen D H, Gao J H, Huang H, Shi T L, Yang Z N. Development of genome-wide DNA polymorphism database for map-based cloning of rice genes. Plant Physiology, 2004, 135(3): 1198-1205.

[21]Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proceedings of the National Academy of Sciences of the United States of America, 1991, 88(21): 9828-9832.

[22]Vossen R H, Aten E, Roos A. High-resolution melting analysis(HRMA)-more than just sequence variant screening. Human Mutatation, 2009, 30(10): 1-7.

[23]Kinoshita T, Takahashi M. The one hundredth report of genetical studies on the rice plant. Linkage studies and future prospects. Journal of the Faculty of Agriculture, 1991, 65(1): 1-61.

[24]Czemmel S, Stracke R, Weisshaar B, Cordon N, Harris N N, Walker A R, Robinson S P, Bogs J. The grapevine R2R3-MYB transcription factor VvMYBF1 regulates flavonol synthesis in developing grape berries. Plant Physiology, 2009, 151(3): 1513-1530.

[25]Matsui K, Umemura Y, Ohme-Takagi M. AtMYBL2, a protein with a single MYB domain, acts as a negative regulator of anthocyanin biosynthesis in Arabidopsis. The Plant Journal, 2008, 55(6): 954-967.

[26]Terrier N, Torregrosa L, Ageorges A, Vialet S, Verriès C, Cheynier V, Romieu C. Ectopic expression of VvMybPA2 promotes proanthocyanidin biosynthesis in grapevine and suggests additional targets in the pathway. Plant Physiology, 2009, 149(2): 1028-1041.

[27]Matus J T, Poupin M J, Cañón P, Bordeu E, Alcalde J A, Arce-Johnson P. Isolation of WDR and bHLH genes related to ?avonoid synthesis in grapevine (Vitis vinifera L.). Plant Molecular Biology, 2010(72): 607-620.

[28]Walker A R, Davison P A, Bolognesi-Winfield A C, James C M, Srinivasan N, Blundell T L, Esch J J, Marks M D, Gray J C. The TRANSPARENT TESTA GLABRA1 locus, which regulates trichome differentiation and anthocyanin biosynthesis in Arabidopsis, encodes a WD40 repeat protein. The Plant Cell, 1999, 11(7): 1337-1350

[29]Kanokporn S, Yukiko M, Mami Y, Kazaki S. A WD-repeat-containing putative regulatory protein in anthocyanin biosynthesis in Perilla frutescens. Plant Molecular Biology, 2002, 50(3): 485-495

[30]De V N, Quattrocchio F, Mol J, Koes R. The an11 locus controlling ?ower pigmentation in petunia encodes a novel WD-repeat protein conserved in yeast, plants, and animals. Genes and Development, 1997, 11: 1422-1434.

[31]Carey C C, Strahle J T, Selinger D A, Chandler V L. Mutations in the pale aleurone color1 regulatory gene of the Zea mays anthocyanin pathway have distinct phenotypes relative to the functionally similar TRANSPARENT TESTA GLABRA1 gene in Arabidopsis thaliana. The Plant Cell, 2004, 16(2): 450-464.

[32]Pang Y, Wenger J P, Saathoff K, Peel G J, Wen J, Huhman D, Allen S N, Tang Y, Cheng X, Tadege M, Ratet P, Mysore K S, Sumner L W, Marks M D, Dixon R A. A WD40 Repeat Protein from Medicago truncatula is necessary for tissue-specific anthocyanin and proanthocyanidin biosynthesis but not for trichome development. Plant Physiology, 2009, 151(3): 1114-1129.

[33]Gonzalez A, Zhao M, Leavitt J M, Lloyd A M. Regulation of the anthocyanin biosynthetic pathway by the TTG1/bHLH/Myb transcriptional complex in Arabidopsis seedlings. The Plant Journal, 2008, 53: 814-827.

[34]Wataru S, Taku O, Keisuke K, Chikara M, Akira S, Minoru M, Kazuhiko N, Masahiko M. The Purple leaf(Pl) locus of rice: The Plw allele has a complex organization and includes two genes encoding basic helix-loop-helix proteins involved in anthocyanin biosynthesis. Plant Cell Physiology, 2001, 42(9): 982-991.

[35]Van N S, Ludwig P. The WD-repeat protein superfamily in Arabidopsis: Conservation and divergence in structure and function. BMC Genomics, 2003, 4: 50.

[36]Maes L, Inze D, Goossens A. Functional specialization of the TRANSPARENT TESTA GLABRA1 network allows differential hormonal control of laminal and marginal trichome initiation in Arabidopsis rosette leaves. Plant Physiology, 2008, 148(3): 1453-1464.

[37]Suganuma T, Pattenden S G, Workman J L. Diverse functions of WD40 repeat proteins in histone recognition. Genes and Development, 2008, 22(10): 1265-1268.

[38]Zhao M, Morohashi K, Hatlestad G, Grotewold E, Lloyd A. The TTG1-bHLH-MYB complex controls trichome cell fate and patterning through direct targeting of regulatory loci. Development, 2008, 135(11): 1991-1999.

[39]Neer E J, Schmidt C J, Nambudripad R, Smith T F. The ancient regulatory-protein family of WD-repeat proteins. Nature, 1994, 371(6495): 297-300.

[40]Smith T F, Gaitatzes C, Saxena K, Neer E J. The WD repeat: A common architecture for diverse functions. Trends in Biochemical Sciences, 1999, 24(5): 181-185.

[41]Li D, Roberts R. WD-repeat proteins: Structure characteristics, biological function, and their involvement in human diseases. Cellular and Molecular Life Sciences, 2001, 58(14): 2085-2097.

[42]Seet B T, Dikic I, Zhou M M, Pawson T. Reading protein modifications with interaction domains. Nature Reviews Molecular Cell Biology, 2006, 7(7): 473-483.

[43]Xu C, Min J. Structure and function of WD40 domain proteins. Protein Cell, 2011, 2(3): 202-214.
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