Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (13): 2685-2694.doi: 10.3864/j.issn.0578-1752.2013.13.007

• PLANT PROTECTION • Previous Articles     Next Articles

Genetic Diversity Analysis of Myzus persicae (Sulzer) in Guizhou Province

LU Zhao-Yun , YANG  Mao-Fa, SHI  Pei-Qiong, LI  Shang-Wei, LIAO  Qi-Rong, SHANG  Sheng-Hua   

  1. 1.Institute of Entomology, Guizhou University/Guizhou Key Laboratory for Plant Pest Management of Mountainous Region,  Guiyang 550025
    2.Guizhou Tobacco Science Research Institute, Guiyang 550081
  • Received:2012-12-13 Online:2013-07-01 Published:2013-03-26

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Abstract: 【Objective】 The objective of this study is to analyze whether there are genetic differences and the degree of difference of Myzus persicae in typical ecological areas of Guizhou, and to provide an important theoretical basis for clearing the population heterogeneity and population differentiation, discovering and revealing the variation rule and mechanism, guiding the pest’s forecast as well as integrated management. 【Method】 SSR molecular marker technology was used for genetic diversity analysis of tobacco aphid populations of 25 major tobacco-growing areas in Guizhou Province. 【Result】 Most of the populations were moderately differentiated, and minority populations were highly differentiated. Through analysis, the relationship of population genetic differentiation, geographic distance and the altitude were that the genetic distance and geographic distance had no significant correlation, genetic similarity and the elevation had no significant correlation either. The cluster analysis showed that the 25 populations could be divided into 3 parts.【Conclusion】The population genetic differentiation is not in conformity with the geographical isolation mode. Genetic differentiation of the 25 populations in Guizhou is complex. The special terrain and climate may be the reason.

Key words: Guizhou Province , Myzus persicae (Sulzer) , microsatellite marker , genetic diversity , genetic differentiation

[1]张广学, 钟铁森. 中国经济昆虫志, 第25册, 同翅目: 蚜虫类 (一). 北京: 科学出版社,1983: 312-314.

Zhang G X, Zhong T S. Economic Insect Fauna of China, Fasc.25, Homoptera: Aphidinea, Part Ⅰ. Beijing: Science Press, 1983: 312-314. (in Chinese)

[2]陈磊, 张春妮, 仵均祥. 不同植物上桃蚜两种体色生物型的DNA多态性研究. 西北农业学报, 2005, 14(4): 127-131.

Chen L, Zhang C N, Wu J X. DNA polymorphism studies on the two body colour bio-types of green peach aphid from different host plants. Acta Agriculturae Boreali-occidentalis Sinica, 2005, 14(4): 127-131. (in Chinese)

[3]Fenton B, Woodford J A T, Malloch G. Analysis of clonal diversity of the peach-potato aphid, Myzus persicae (Sulzer), in Scotland, UK and evidence for the existence of a predominant clone. Molecular Ecology, 1998, 7(11): 1475-1487.

[4]Vorburger C. Temporal dynamics of genotypic diversity reveal strong clonal selection in the aphid Myzus persicae. Journal of Evolutionary Biology, 2006, 19(1): 97-107.

[5]杨效文, 张孝羲, 陈晓峰, 王瑛. 我国烟蚜种群分化的RAPD分析. 昆虫学报, 1999, 42(4): 372-380.

Yang X W, Zhang X X, Chen X F, Wang Y. RAPD-PCR analysis of population differentiation of green peach aphid in China. Acta Entomologica Sinica, 1999, 42(4): 372-380. (in Chinese)

[6]杨效文, 张孝羲, 陈晓峰, 王瑛. 不同寄主植物上烟蚜DNA多态性的RAPD-PCR 分析. 植物保护学报, 1999, 26(2): 147-153.

Yang X W, Zhang X X, Chen X F, Wang Y. On the RAPD-PCR analysis of DNA polymorphism of green peach aphid form different host plants. Acta Phytophylacica Sinica, 1999, 26(2): 147-153. (in Chinese)

[7]刘永刚, 漆永红, 李惠霞, 郭满库, 吕和平, 张俊莲, 贺春贵. 甘肃不同地理种群桃蚜的遗传相似性. 中国农业科学, 2010, 43(15): 3134-3142.

Liu Y G, Qi Y H, Li H X, Guo M K, Lü H P, Zhang J L, He C G. Genetic similarity of Myzus persicae from different geographical populations in Gansu Province. Scientia Agricultura Sinica, 2010, 43(15): 3134-3142. (in Chinese)

[8]王绍辉. 利用微卫星标记对蚜虫种群遗传多样性的初步研究[D]. 西安: 陕西师范大学, 2003.

Wang S H. Studies on the population diversity of Aphids using microsatellite marker[D]. Xi’an: Shanxi Normal University, 2003. (in Chinese)

[9]李子忠, 汪廉敏. 贵州农林昆虫志 (卷4, 同翅目: 叶蝉科). 贵阳: 贵州科技出版社, 1992: 6-19.

Li Z Z, Wang L M. Agriculture and Forestry Insect Fauna of Guizhou, Vol.4, Homoptera Cicadellidae. Guiyang: Guizhou Science and Technology Press, 1992: 6-19. (in Chinese)

[10]Sloane M A, Sunnucks P, Wilson A C C, Hales D F. Microsatellite isolation, linkage group identification and determination of recombination frequency in the peach-potato aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae). Genetic Research, 2001, 77(3): 251-260.

[11]安瑞生, 谭声江, 陈晓峰. 小型昆虫DNA提取时匀浆方法的改进. 昆虫知识, 2002, 39(4): 311-312.

An R S, Tan S J, Chen X F. Improvement in grinding tissue during extracting DNA from small insects. Entomological Knowledge, 2002, 39(4): 311-312. (in Chinese)

[12]Wilson A C C, Massonnet B, Simon J C. Cross-species amplification of microsatellite loci in aphids: assessment and application. Molecular Ecology Notes, 2004, 4(1): 104-109.

[13]吕召云, 杨茂发, 商胜华. 烟蚜2种微卫星标记银染方法的比较. 中国农学通报, 2011, 27(24): 273-276.

Lü Z Y, Yang M F, Shang S H. Comparison of two silver staining methods for polyaerylamide gels electrophoresis by using SSR markers on Myzus persicae (Sulzer). Chinese Agricultural Science Bulletin, 2011, 27(24): 273-276. (in Chinese)

[14]Wright S. The genetical structure of population. Annals of Eugenics, 1951, 15: 323-354.

[15]Wright S. Evolution and the genetic populations variability within and among natural populations. Chicago and London: University of Chicago Press, 1978, 4: 79-103.

[16]Whitlock M C, McCauley D E. Indirect measures of gene flow and migration: FST≠1/(4Nm+1). Heredity, 1999, 82: 117-125.

[17]Delmotte F, Leterme N, Gauthier J P, Rispe C, Simon J C. Genetic architecture of sexual and asexual populations of the aphid Rhopalosiphum padi based on allozyme and microsatellite markers. Molecular Ecology, 2002, 11(4): 711-723.

[18]Simon J C, Rispe C, Sunnucks P. Ecology and evolution of sex in aphids. Trends in Ecology & Evolution, 2002, 17(1): 34-39.

[19]李厥鲁. 试析我国不同生态烟区烟蚜发生特点及其防治区划. 中国烟草, 1994(4): 1-8.

Li J L. The occurrence characteristics and control regionalization of Myzus persicae in different ecological areas of China. China Tobacco, 1994(4): 1-8. (in Chinese)

[20]吕召云, 杨茂发, 李尚伟. 基于微卫星标记的桃蚜在烟草和甘蓝上的遗传多样性. 贵州农业科学, 2011, 39(12): 122-125.

Lü Z Y, Yang M F, Li S W. Gentic diversity of Myzus persicae in tobacco and cabbage fields based on microsatellite marker. Guizhou Agricultural Sciences, 2011, 39(12): 122-125. (in Chinese)

[21]Guillemaud T, Mieuzet L, Simon J C. Spatial and temporal genetic variability in French populations of the peach-potato aphid, Myzus persicae. Heredity, 2003, 91: 143-152.

[22]蔡青年, 胡熳华, 王宇, 谷平. 蚜虫种群遗传多样性的影响因素及分子基础. 昆虫知识, 2004, 41(4): 285-290.

Cai Q N, Hu M H, Wang Y, Gu P. Influence factors and molecular basis of the genetic diversity of aphid populations. Entomological Knowledge, 2004, 41(4): 285-290. (in Chinese)

[23]Guo W, Shen Z R, Li Z H, Gao L W. Migration and population genetics of the grain aphid Macrosiphun miscanti (Takahashi) in relation to the geographic distance and gene flow. Progress in Natural Science, 2005, 15(11): 1000-1004.

[24]Rispe C, Pierre J S, Simon J C, Gouyon P H. Models of sexual and asexual coexistence in aphids based on constraints. Journal of Evolutionary Biology, 1998, 11(6): 685-701．
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