Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (9): 1892-1902.doi: 10.3864/j.issn.0578-1752.2013.09.017

• STORAGE·FRESH-KEEPING·PROCESSING • Previous Articles     Next Articles

Determination of Flavonoids in 10 Pummelo and Pummelo Hybrid Fruits by Ultra Performance Liquid Chromatography

 FANG  Bo, ZHAO  Qi-Yang, XI  Wan-Peng, ZHOU  Zhi-Qin, JIAO  Bi-Ning   

  1. 1.College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716
    2.Key Laboratory of Horticulture Science for Southern Mountainous Regions，Ministry of Education, Chongqing 400715
    3.Laboratory of Risk Assessment for Citrus Quality and Safety of Ministry of Agriculture/Citrus Research Institute, Southwest University, Chongqing 400712
    4.National Citrus Engineering Research Center, Chongqing 400712
  • Received:2012-12-28 Online:2013-05-01 Published:2013-02-28

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Abstract: 【Objective】This study aimed to determine 11 flavonoids in 10 pummelos and pummelo hybrids fruits and analyze flavonoids types and content in varieties of fruits by ultra performance liquid chromatograph (UPLC) method.【Method】The separation was carried on a ACQUITY UPLC BEH C18 (2.1 mm×100 mm，1.7 μm, column temperature 35℃). Methanol (A)/0.1% formic acid/water (B) mixture was used as the mobile phase at a flow rate of 0.3 mL•min-1 through gradient elution: (1) 0-5 min, 90%-80% A; (2) 5-10 min, 80%-30% A; (3) 10-15 min, 30%-20% A; (4) 15-16 min, 20%-90% A. 【Result】 The flavonoids types and content in citrus fruit depend on genetic genotypes. Naringin is a major flavonoid in pummel. Naringin and neohesperidin are major flavonoids in grapefruit. Naringin content is higher in Riored fruit (Peel: 27.5 mg•g-1 DW, Pulp: 2.73 mg•g-1 DW) and neohesperidin content is higher in Cocktail (Peel: 14.93 mg•g-1 DW, Pulp: 0.70 mg•g-1 DW). The accumulation of flavonoids in hybrid fruits were affected by their parents.【Conclusion】UPLC method was effective in qualitative and quantitative analysis of flavonoids in citrus fruits. The flavonoids were abundant in pummelo fruits and the difference of flavonoids composition in diverse citrus fruits was significant.

Key words: ultra performance liquid chromatography , pummelo , flavonoids , determination

[1]Matook S M, Toshihiko S. Antioxidant and antimicrobial activities of the methanol extracts from pummelo (Citrus grandis Osbeck) fruit albedo tissues. European Food Research and Technology, 2006, 224:39-47.

[2]Huang Y S, Ho S C. Polymethoxy flavones are responsible for the anti-inflammatory activity of citrus fruit peel. Food Chemistry, 2010, 119(3): 868-873.

[3]Wilmsen P K, Spada D S, Salvador M. Antioxidant activity of the flavonoid hesperidin in chemical and biological systems. Journal of Agricultural and Food Chemistry, 2005, 53(12):4757-4761.

[4]Manthey J A, Guthrie N. Antiproliferative activities of citrus flavonoids against six human cancer cell lines. Journal of Agricultural and Food Chemistry, 2002, 50(1):5837-5843.

[5]Tripoli E, La G M, Giammanco S, Di M D, Giammanco M. Citrus ?avonoids: molecular structure, biological activity and nutritional properties: A review. Food Chemistry, 2007, 104(2):466-479.

[6]Yusof S, Mohd G H, Swee K G. Naringin content in local citrus fruits. Food Chemistry, 1990, 37:113-121.

[7]Ortuño A, García P D, Fuster M D, Pérez M L, Sabater F, Porras I, García L, Del Rio J A. Flavanone and nootkatone levels in different varieties of grapefruit and pummelo. Journal of Agricultural and Food Chemistry, 1995,43:1-5.

[8]Mark A B, Carl E V. Biosynthesis of naringin and prunin in detached grapefruit. Phytochemistry, 1989, 28(6):1627-1630.

[9]赵雪梅, 叶兴乾, 席屿芳, 朱大元, 蒋山好. 胡柚皮中的黄酮类化合物. 中草药, 2003,1(34):11-13.

Zhao X M, Ye X Q, Xi Y F, Zhu D Y, Jiang S H. Flavonoids in peels of Citrus changshan-huyou. Chinese Traditional and Herbal Drugs, 2003, 1(34):11-13. (in Chinese)

[10]Zhang M X, Duan C Q, Zang Y Y, Huang Z W, Liu G J. The flavonoid composition of flavedo and juice from the pummelo cultivar (Citrus grandis (L.) Osbeck) and the grapefruit cultivar (Citrus paradisi) from China. Food Chemistry, 2011, 4(129):1530-1536.

[11]Nogata Y, Sakamoto K, Shiratsuchi H, Ishii T, Yano M, Ohta H. Flavonoid composition of fruit tissues of citrus species. Bioscience, Biotechnology and Biochemistry, 2006, 70(1):178-192.

[12]Kawaii S, Tomono Y, Katase Y, Ogawa K, Yano M. HL-60 differentiating activity and flavonoid content of the readily extractable fraction prepared from citrus juices. Journal of Agricultural and Food Chemistry, 1999, 47(1):128-135.

[13]Wang Y C, Chuang Y C, Hsu H W. The flavonoid, carotenoid and pectin content in peels of citrus cultivated in Taiwan. Food Chemistry, 2008, 106(1):277-284.

[14]张元梅, 周志钦, 孙玉敬, 沈妍, 钟烈洲, 乔丽萍, 叶兴乾. 高效液相色谱法同时测定柑橘果实中 18 种类黄酮的含量. 中国农业科学, 2012,45(17):3558-3565.

Zhang Y M, Zhou Z Q，Sun Y J, Shen Y, Zhong L Z, Qiao L P, Ye X Q. Simultaneous determination of 18 flavonoids in Citrus fruits by high-performance liquid chromatography. Scientia Agricultura Sinica, 2012, 45(17):3558-3565. (in Chinese)

[15]Gruz J, Novak O, Stmad M. Rapid analysis of phenolic acids in beverages by UPLC-MS/MS. Food Chemistry, 2008, 111(3): 789-794.

[16]国家药典委员会.中国药典（二部）：附录XIX A. 2010 版. 北京：中国医药科技出版社, 2010.

Chinese Pharmacopoeia Committee. Chinese Pharmacopoeia (II), Appendices XIX A (2010). Beijing: China Medical Science and Technology Press, 2010. (in Chinese)

[17]Yan Z X, Yang X H, Wu J B, Su H, Chen C, Chen Y. Qualitative and quantitative analysis of chemical constituents in traditional Chinese medicinal formula Tong-Xie-Yao-Fang by high-performance liquid chromatography/diode array detection/electrospray ionization tandem  mass spectrometry. Analytica Chimica Acta, 2011, 691:110-118.

[18]吴厚玖, 汪晓红, 王华, 孙志高, 焦必林, 蒋大斌, 唐忠海, 余恩惠. 柚和葡萄柚果实发育过程中果皮柚苷的消长规律研究. 中国农业科学, 1995, 28(5): 73-77.

Wu H J, Wang X H, Wang H, Sun Z G, Jiao B L, Jiang D B, Tang Z H, Yu E H. A study on the growth and decline pattern of naringin in peel of developing fruits of shaddocks and grapefruit. Scientia Agricultura Sinica, 1995, 28(5): 73-77. (in Chinese)

[19]Wang Y C, Chuang Y C, Ku Y H. Quantitation of bioactive compounds in citrus fruits cultivated in Taiwan. Food Chemistry, 2007, 102: 1163-1171. 

[20]Li C M, Gu H F, Dou H L, Zhou L M. Identi?cation of ?avanones from peel of Citrus changshan-huyou Y. B. Chang by HPLC-MS  and NMR. European Food Research and Technology, 2007, 225: 777-782.

[21]Ooghe W C, Ooghe S J, Detavernier C M, Huyghebaert A. Characterization of orange juice (Citrus sinensis) by flavanone glycosides. Journal of Agricultural and Food Chemistry, 1994, 42:2183-2190.

[22]Del Río J A, Fuster M D, Gómez P, Porras I, García Lidón A, Ortuño A. Citrus limon: a source of ?avonoids of pharmaceutical interest. Food Chemistry, 2004 , 84: 457-461.

[23]Pan G G, Kilmartin P A, Smith B G, Melton L D. Detection of orange juice adulteration by tangelo juice using multivariate analysis of polymethoxylated flavones and carotenoids. Journal of the Science of Food and Agriculture, 2002, 82(4):421-427.

[24]聂继云, 吕德国, 李静, 刘凤之, 李海飞, 王昆. 22 种苹果种质资源果实类黄酮分析. 中国农业科学, 2010, 43(21): 4455-4462.

Nie J Y, Lü D G, Li J, Liu F Z, Li H F, Wang K. A Preliminary study on the flavonoids in fruits of 22 apple germplasm resources. Scientia Agricultura Sinica, 2010, 43(21): 4455-4462. (in Chinese)

[25]李卫星, 甄珍, 周春华, 张秀萍, 于建友, 陈鹏. 银杏雄株叶片和花粉主要类黄酮成分含量分析.中国农业科学, 2010, 43(13): 2775-2783.

Li W X, Zhen Z, Zhou C H, Zhang X P, Yu J Y, Chen P. Flavonoid content of the leaves and pollen of male Ginkgo biloba plants. Scientia Agricultura Sinica, 2010, 43(13): 2775-2783. (in Chinese)

[26]Spacil Z, Novakova L, Solich P. Analysis of phenolic compounds by high performance liquid chromatography and ultra performance liquid chromatography. Talanta, 2008, 76(1): 189-199.

[27]Ramful D, Tarnus E , Aruoma O I, Bourdon E, Bahorun T. Polyphenol composition, vitamin C content and antioxidant capacity of Mauritian citrus fruit pulp. Food Research International, 2011, 44(7):2088-2099.

[28]Goulas V, Manganaris G A. Exploring the phytochemical content and the antioxidant potential of Citrus fruits grown in Cyprus. Food Chemistry, 2012, 131:39-47.

[29]Davide B, Ersilia B, Corrado C, Ugo L, Giuseppe G. Elucidation of the ?avonoid and furocoumarin composition and radical-scavenging activity of green and ripe chinotto (Citrus myrtifolia Raf.) fruit tissues, leaves and seeds. Food Chemistry, 2011, 129:1504-1512.
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