Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (9): 1892-1902.doi: 10.3864/j.issn.0578-1752.2013.09.017

• STORAGE·FRESH-KEEPING·PROCESSING • Previous Articles     Next Articles

Determination of Flavonoids in 10 Pummelo and Pummelo Hybrid Fruits by Ultra Performance Liquid Chromatography

 FANG  Bo, ZHAO  Qi-Yang, XI  Wan-Peng, ZHOU  Zhi-Qin, JIAO  Bi-Ning   

  1. 1.College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716
    2.Key Laboratory of Horticulture Science for Southern Mountainous Regions，Ministry of Education, Chongqing 400715
    3.Laboratory of Risk Assessment for Citrus Quality and Safety of Ministry of Agriculture/Citrus Research Institute, Southwest University, Chongqing 400712
    4.National Citrus Engineering Research Center, Chongqing 400712
  • Received:2012-12-28 Online:2013-05-01 Published:2013-02-28

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Abstract: 【Objective】This study aimed to determine 11 flavonoids in 10 pummelos and pummelo hybrids fruits and analyze flavonoids types and content in varieties of fruits by ultra performance liquid chromatograph (UPLC) method.【Method】The separation was carried on a ACQUITY UPLC BEH C18 (2.1 mm×100 mm，1.7 μm, column temperature 35℃). Methanol (A)/0.1% formic acid/water (B) mixture was used as the mobile phase at a flow rate of 0.3 mL•min-1 through gradient elution: (1) 0-5 min, 90%-80% A; (2) 5-10 min, 80%-30% A; (3) 10-15 min, 30%-20% A; (4) 15-16 min, 20%-90% A. 【Result】 The flavonoids types and content in citrus fruit depend on genetic genotypes. Naringin is a major flavonoid in pummel. Naringin and neohesperidin are major flavonoids in grapefruit. Naringin content is higher in Riored fruit (Peel: 27.5 mg•g-1 DW, Pulp: 2.73 mg•g-1 DW) and neohesperidin content is higher in Cocktail (Peel: 14.93 mg•g-1 DW, Pulp: 0.70 mg•g-1 DW). The accumulation of flavonoids in hybrid fruits were affected by their parents.【Conclusion】UPLC method was effective in qualitative and quantitative analysis of flavonoids in citrus fruits. The flavonoids were abundant in pummelo fruits and the difference of flavonoids composition in diverse citrus fruits was significant.

Key words: ultra performance liquid chromatography , pummelo , flavonoids , determination

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