意大利蜜蜂工蜂毒腺内蜂毒与电取蜂毒蛋白质组比较

doi:10.3864/j.issn.0578-1752.2013.07.016

Abstract

Abstract: 【Objective】The objective of this study is to investigate bee venom composition and difference by comparison of bee venom collected from venom glands and electrical stimulated of Italian bees (Apis mellifera ligustica), and to provide a theoretical basis for pharmaceutical application.【Method】The proteome between bee venom collected directly from the venom glands and artificially electrical stimulation were compared using gel-based (one-dimensional gel electrophoresis, 1-DE, two-dimensional gel electrophoresis, 2-DE) and gel-free proteomics approaches, mass spectrometry, and bioinformatics.【Result】In the gland venom, 19, 11 and 14 proteins were identified by 1-DE, 2-DE and shotgun analysis, respectively, which correspond to 30 nonredundant proteins. They were mainly classified as bee venom toxins (50%), antioxidant systems, protein folding and molecular transporters (50%). In construct, in electrical stimulated venom, 12, 3 and 7 proteins were identified, respectively, by 1-DE, 2-DE and shotgun analysis. They were classified into 14 nonredundant proteins, which the major components were venom toxins (93%). Notably, phospholipase A2-like protein was identified for the first time in two forms of bee venom and peptidyl-prolyl cis-trans isomerase was identified only in the gland venom for the first time. The abundance of apamin preproprotein and secapin in the gland venom were significantly higher than those in the electrical stimulated venom. However, phospholipase A-2, venom dipeptidyl peptidase IV precursor, venom allergen acid phosphatase and mast cell degranulating peptide had higher abundance in the electrical stimulated venom than those in the gland venom.【Conclusion】 The venom collected from venom glands contains more protein species, but the abundance of the toxin proteins in electrical stimulated venom are no less than the venom collected from the glands. As the pharmacological components are mainly contained in the toxin proteins, the electrical stimulated venom is a convenient and effective way for utilization. The identified new proteins significantly extend the knowledge of bee venom composition. The result may provide a theoretical and practical basis for future rational use of the honeybee venom.

Key words: Apis mellifera ligustica , bee venom directly collected from venom glands , bee venom collected from electrical stimulated , proteome , gel-electrophoresis , gel-free

LI Rong-Li, ZHANG Lan, HAN Bin, FANG Yu, FENG Mao, ZHOU Tian-E, LI Jian-Ke. Proteome Comparison of Honeybee (Apis mellifera ligustica) Worker Venom Between Collected from Venom Glands and Electrical Stimulated[J].Scientia Agricultura Sinica, 2013, 46(7): 1448-1462.

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Proteome Comparison of Honeybee (Apis mellifera ligustica) Worker Venom Between Collected from Venom Glands and Electrical Stimulated

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