Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (7): 1441-1447.doi: 10.3864/j.issn.0578-1752.2013.07.015

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

miR-133a Targets BIRC5 to Regulate Its Gene Expression in Chicken

 WANG  Xing-Guo, SHAO  Fang, GONG  Dao-Qing, LU  Xiang-Yun, GU  Zhi-Liang   

  1. 1.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu;
    2.Department of Life Science and Technology, Changshu Institute of Technology, Changshu 215500, Jiangsu
  • Received:2012-10-08 Online:2013-04-01 Published:2013-01-18

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Abstract: 【Objective】This study was conducted to investigate the expression pattern of miR-133a in various chicken tissues, and find whether baculoviral IAP repeat containing 5 (BIRC5) is the target gene of miR-133a.【Method】The bioinformatics methods were used to predict the target genes of miR-133a. Real-time PCR was used to detect the expression patterns of miR-133a of various tissues in chicken. Dual-luciferase reporter assays and site mutation assays were used to verify whether BIRC5 is a target gene of miR-133a. 【Result】Two hundred and eighty-seven genes of 11891 genes from 3′UTR database were predicted as target genes of miR-133a. The expression patterns of miR-133a in various chicken tissues showed that miR-133a was highly expressed in muscle especially in skeletal muscle. Reporter assays showed that BIRC5 was the target gene of miR-133a and the site mutation assays validated the target site of miR-133a in BIRC5. 【Conclusion】 miR-133a is a miRNA related with skeletal muscle development of chicken, and BIRC5 is a bona fide target gene in chicken.

Key words: chicken , skeletal muscle , miR-133a , BIRC5 gene

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