Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (4): 800-809.doi: 10.3864/j.issn.0578-1752.2013.04.014

• STORAGE·FRESH-KEEPING·PROCESSING • Previous Articles     Next Articles

The “Value” Expression Pattern and Technical Procedure for Genetically Modified Organism Detection Reference Materials

 LI  Yun-Jing, SONG  Gui-Wen, SHEN  Ping, ZHAO  Xin, ZHOU  Yun-Long   

  1. 1.中国农业科学院油料作物研究所/农业部油料作物生物学与遗传育种重点实验室,武汉 430062
    2.农业部科技发展中心,北京 100122
  • Received:2012-09-04 Online:2013-02-15 Published:2013-01-04

Abstract: This paper describes the worldwide production, characterization and application status of genetically modified organism (GMO) detection reference materials (RMs). The different "value" expression patterns and technical approaches of current GMO detection RMs were systematically compared. The advantages and disadvantages of each "value" expression pattern were also elucidated. According to the comparison results, it was suggested that the most suitable pattern of "value" expression and technical approaches of RMs for GMO safety supervision in China. These will provide references for the establishment of characterization system and production and application platform for GMO detection RMs.

Key words: genetically modified organism(GMO) , reference material , value

[1]Zhang D B, Guo J C. The development and standardization of testing methods for genetically modified organisms and their derived products. Journal of Integrative Plant Biology, 2011, 53(7):539-551.

[2]沈平, 刘信, 张明, 张大兵, 厉建萌, 卢长明, 王晶, 杨立桃, 张景六, 李飞武, 李允静. 农业部1485号公告-19-2010《转基因植物及其产品成分检测 基体标准物质候选物鉴定方法》[S].北京:中国农业出版社, 2010.

Shen P, Liu X, Zhang M, Zhang D B, Li J M, Lu C M, Wang J, Yang L T, Zhang J L, Li F W, Li Y J. Ministry of Agriculture, 1485 announcement-19-2010, Methods for identification of matrix reference material candidate[S]. Beijing: China Agriculture Press, 2010. (in Chinese)

[3]Trapmann S, Catalani P, Conneely P, Corbisier P, Gancberg D, Hannes E, Le Guern L, Kramer G N, Prokisch J, Robouch P, Schimmel H, Zeleny R, Pauwels J, Van den E G, Weighardt F, Mazzara M, Anklam E. Certification report[R]. The Certification of Reference Materials of Dry-mixed Soya Powder with different Mass Fractions of Roundup ReadyTM Soya IRMM-410S. IRMM Information Series ISBN 92-894-3725-1, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Retieseweg, B-2440 Geel: 2002.

[4]周云龙, 卢长明, 刘信, 曹应龙, 宋贵文, 沈平, 吴刚, 杨立桃, 王晶, 王江, 李允静, 李飞武, 赵欣. 农业部1782号公告-8-2012《转基因植物及其产品成分检测基体标准物质制备技术规范》[S]. 北京:中国农业出版社, 2012.

Zhou Y L, Lu C M, Liu X, Cao Y L, Song G W, Shen P, Wu G, Yang L T, Wang J, Wang J, Li Y J, Li F W, Zhao X. Ministry of Agriculture, 1782 announcement-8-2012, Detection of genetically modified plants and derived products-Technical specification for manufacture of matrix reference material[S]. Beijing: China Agriculture Press, 2012. (in Chinese)

[5]李亮, 王晶, 隋志伟, 臧超, 余笑波. 转基因定量检测用质粒分子标准物质研究进展. 生物技术通报, 2012(2): 48-52.

Li L, Wang J, Sui Z W, Zang C, Yu X B. Advances in reference plasmids for the quantitative detection of genetically modified crops. Biotechnology Bulletin, 2012(2): 48-52. (in Chinese)

[6]董莲华, 赵正宜, 李亮, 隋志伟, 王晶. 转基因植物标准物质研究进展. 农业生物技术学报, 2012, 20(2): 203-210.

Dong L H, Zhao Z Y, Li L, Sui Z W, Wang J. Advance in developing genetically modified plant reference material. Journal of Agricultural Biotechnology, 2012, 20(2): 203-210. (in Chinese)

[7]Wang S, Li X, Yang L T, Shen K L, Zhang D B. Development and in-house validation of a reference molecule pMIR604 for simplex and duplex event-specific identification and quantification of GM maize MIR604. European Food Research and Technology, 2009, 230: 239-248.

[8]柳方方, 张玲, 宋贵文, 曹应龙, 盛灵慧, 黄峥, 王晶, 秦培勇, 卢长明. 转基因油菜TOPAS19/2 质粒分子多家协同实验及不确定度评定. 中国农业科技导报,2012, 14( 3): 31-35.

Liu F F, Zhang L, Song G W, Cao Y L, Sheng L H, Huang Z, Wang J, Qin P Y, Lu C M. Cooperative experiment and uncertainty analysis   for transgenic rapeseed TOPAS 19 /2 plasmid molecular. Journal   of Agricultural Science and Technology, 2012, 14(3): 31-35. (in Chinese)

[9]Trapmann S, Charoud-Got J, Conneely P, Contreras L M C, Corbisier P, Kortekaas A M, Raffaelli B, Seghers J, Vincent S. Certification report[R]. The Certification of Reference Materials of potato powder with different mass fraction of the event AM04-1020, ISSN 1018-5593. Belgium: European Union, 2011.

[10]Corbisier P, Broeders S, Charels D, Trapmann S, Vincent S, Emons H. Certification report[R]. Certification of plasmidic DNA containing MON 810 maize DNA fragments Certified Reference Materials ERM®-AD413, doi:10.278/49996, Belgium: European Communities, 2007.

[11]Caprioara-Buda M, Corbisier P, Gancberg D, Broeders S, Tranpmann S, Mazoua S, Matayron G, Emons H. Certification report[R]. Certification of plasmidic DNA containing 98140 maize DNA fragments Certified Reference Materials ERM®-AD427, doi:10.2787/ 38563, Belgium: European Union, 2011.

[12]Jeynov B, de Andrade E, Broothaerts W, Corbisier P, Mazoua S, Merveillie A, Trapmann S, Emons H. Certification report[R]. Certification of plasmidic DNA containing NK603 maize DNA fragments Certified Reference Materials ERM®-AD415, doi:10.2787/37591, Belgium: European Union, 2011.

[13]Corbisier P, Broeders S, Meeus N, Mazoua S, Meyer W, Trapmann S, Emons H. Certification report[R]. Certification of plasmidic DNA containing 356043 soybean DNA fragments Certified Reference Materials ERM®-AD425, Doi:10.2787/36019, Belgium: European Union, 2011.

[14]Clapper G, Cantrill R. Certification report[R]. The certification of Conventional and Roundup Ready® (RT73) Canola Seed Reference Materials.  AOCS, Champaign, IL, USA, 2004.

[15]Clapper G, Cantrill R. Certification report[R]. The certification of T45 Canola Leaf DNA Reference Materials. AOCS, Champaign, IL, USA, 2008.

[16]Kirk M R, Doris A D, Deanne L W, Larry R H. Statistical considerations in seed purity testing for transgenic traits. Seed Science Research, 2001, 11: 101-119.

[17]Kuribara H, Shindo Y, Matsuoka T, Takubo K, Futo S, Aoki N, Hirao T, Akiyama H, Goda Y, Toyoda M, Hino A. Novel reference molecules for quantitation of genetically modified maize and soybean. Journal of AOAC International, 2002, 85(5): 1077-1089.

[18]Shindo Y, Kuribara H, Matsuoka T, Futo S, Sawada C, Shono J, Akiyama H, Goda  Y, Toyoda M, Hino A. Validation of real-time PCR analyses for line-specific quantitation of genetically modified maize and soybean using new reference molecular. Journal of AOAC International, 2002, 85(5): 1119-1126.

[19]European Commission. Commission recommendation (EC) N 787/2004 of 4.10.2004 on technical guidance for sampling and detection of genetically modified organisms and material produced from genetically modified organisms as or in products in the context of Regulation (EC) No 1830/2003. Official Journal of the European Union, 2004, L348: 18-26.

[20]European Commission. Commission regulation (EC) No.1829/2003 of September 22, 2003, Concerning on genetically modified food and feed. Official Journal of the European Union, 2003, L268: 1-23.

[21]European Commission. Commission regulation (EC) No. 1830/2003 of September 22, 2003, Concerning the traceability of food and feed products produced from genetically modified organisms and amending Directive 2001/18/EC. Official Journal of the European Union, 2003, L268: 24-28.

[22]Herrera J A. International law and GMOs: can the precautionary principle protect biological diversity?[EB/OL].[Cited 26 June 2008]. Available at http://www.ejournal.unam.mx/bmd/bolmex118/BMD000011804. pdf.

[23]Matsuoka T. GMO labeling and detection methods in Japan. APEC-JIRCAS Joint Symposium and Workshop on Agricultural Biotechnology, 2001.

[24]Ministry of Agriculture and Forestry of South Korea. Guidelines for Labeling of Genetically Modified Agricultural Products. MAF Notification, 31, 2000.

[25]Bonfini L, Heinze P, Kay S, Van den E G. Review of GMO detection and quantification techniques. Ispra, Italy: Institute for Health and Consumer Protection, Food Products and Consumer Goods Unit, 2002: 1-67.

[26]Wu G, Wu Y H, Xiao L, Lu C M. Event-specific qualitative and quantitative PCR detection of genetically modified rapeseed Topas 19/2. Food Chemistry, 2009, 112: 232-238.

[27]Wang X M, Teng D, Yang Y L, Tian F, Guan Q F, Wang J H. Construction of a reference plasmid molecule containing eight targets for the detection of genetically modified crops. Applied Microbiology and Biotechnology, 2011, 90: 721-731.

[28]Li X, Shen K L, Yang L T, Wang S, Pan L W, Zhang D B. Applicability of a novel reference molecule suitable for event-specific detections of maize NK603 based on both 5’ and 3’ flanking sequences. Food Control, 2010, 21: 927-934.

[29]Cao Y L, Wu G, Wu Y H, Nie S J, Zhang L, Lu C M. Characterization of the Transgenic Rice Event TT51-1 and Construction of a Reference Plasmid. Journal of Agricultural and Food Chemistry. 2011, 59: 8550-8559.
[1] LI Jun, SHAN LuYing, XIAO Fang, LI YunJing, GAO HongFei, ZHAI ShanShan, WU Gang, ZHANG XiuJie, WU YuHua. Development of A Set of Matrix Reference Materials in Different Mass Fractions of Genetically Modified Maize MON87427 [J]. Scientia Agricultura Sinica, 2023, 56(8): 1444-1455.
[2] ZHU YouYun, ZENG YuLing, LI Bo, YUAN YuJie, ZHOU Xing, LI QiuPing, HE ChenYan, CHEN Yong, WANG Li, CHENG Hong, ZHOU Wei, TAO YouFeng, LEI XiaoLong, REN WanJun, DENG Fei. Effect of Post-Anthesis Shading Stress on Eating Quality of Indica Rice in Chengdu Plain [J]. Scientia Agricultura Sinica, 2023, 56(3): 430-440.
[3] XU JiuKai, YUAN Liang, WEN YanChen, ZHANG ShuiQin, LI YanTing, LI HaiYan, ZHAO BingQiang. Nitrogen Fertilizer Replacement Value of Livestock Manure in the Winter Wheat Growing Season [J]. Scientia Agricultura Sinica, 2023, 56(2): 300-313.
[4] YIN Chang, ZHU Mo, CHEN YanRu, TONG ShiFeng, ZHAO GuiPing, LIU Yang. Assessment of Genomic Selection Accuracy for Slaughter Traits in Broilers Based on Microarray and Imputed Sequencing Data [J]. Scientia Agricultura Sinica, 2023, 56(15): 3032-3039.
[5] LI ShaoHui, ZHAO Wei, LIU SongYan, LI PengLiang, ZHANG AiXia, LIU JingKe. Aroma Characteristics of Foxtail Millet Varieties from Different Ecological Regions by Analysis of SDE-GC-MS Combined with OPLS-DA [J]. Scientia Agricultura Sinica, 2023, 56(13): 2586-2596.
[6] WANG YiDan,YANG FaLong,CHEN DiShi,XIANG Hua,REN YuPeng. One-Step Multiple TaqMan Real-time RT-PCR for Simultaneous Detection of Swine Diarrhea Viruses [J]. Scientia Agricultura Sinica, 2023, 56(1): 179-192.
[7] ZHU DaWei,ZHANG LinPing,CHEN MingXue,FANG ChangYun,YU YongHong,ZHENG XiaoLong,SHAO YaFang. Characteristics of High-Quality Rice Varieties and Taste Sensory Evaluation Values in China [J]. Scientia Agricultura Sinica, 2022, 55(7): 1271-1283.
[8] WANG ShuTing,KONG YuGuang,ZHANG Zan,CHEN HongYan,LIU Peng. SPAD Value Inversion of Cotton Leaves Based on Satellite-UAV Spectral Fusion [J]. Scientia Agricultura Sinica, 2022, 55(24): 4823-4839.
[9] LIU Hong,GUO YuJie,XU Xiong,LI Xia,ZHANG HongRu,QI LiWei,SUN XueMei,ZHANG ChunHui. Preparation, Physicochemical Characterization and Bioactivity Comparison of Different Livestock and Poultry Bone Peptides [J]. Scientia Agricultura Sinica, 2022, 55(13): 2629-2642.
[10] LU BingLin,CHE ZongXian,ZHANG JiuDong,BAO XingGuo,WU KeSheng,YANG RuiJu. Effects of Long-Term Intercropping of Maize with Hairy Vetch Root Returning to Field on Crop Yield and Nitrogen Use Efficiency Under Nitrogen Fertilizer Reduction [J]. Scientia Agricultura Sinica, 2022, 55(12): 2384-2397.
[11] ZHANG YuanYuan,LIU WenJing,ZHANG BinBin,CAI ZhiXiang,SONG HongFeng,YU MingLiang,MA RuiJuan. Characterization of the Lactone Volatile Compounds in Different Types of Peach (Prunus persica L.) Fruit and Evaluations of Their Contributions to Fruit Overall Aroma [J]. Scientia Agricultura Sinica, 2022, 55(10): 2026-2037.
[12] WEI QiHang,FENG Yao,MA QianQian,LI YanLi,LIU YuanWang,LI ZhaoJun,REN YanFang. Application Effect of Fungi Promoting Secondary Fermentation in Composting [J]. Scientia Agricultura Sinica, 2021, 54(24): 5240-5250.
[13] REN JiaXin,LIU Jing,CHEN XuanJing,ZHANG YueQiang,ZHANG Yong,WANG Jie,SHI XiaoJun. Variation of Available Phosphorus in Purple Soil and Its Effects on Crop Yield of Rice-Wheat Rotation Under Long-Term Fertilizations [J]. Scientia Agricultura Sinica, 2021, 54(21): 4601-4610.
[14] CHEN GuangJi,XIONG XianQin,HE RunXia,TIAN Xiong,SHEN YingLong,ZOU XiaoMin,YANG Hong,SHANG YiShun,ZHAO MingKun,LI XiaoDong,LI ShiGe,ZHANG Rong,SHU JianHong. Evaluation of Feeding Value for Whole Broussonetia papyrifera Silage in Diet of Wuchuan Black Beef Cattle [J]. Scientia Agricultura Sinica, 2021, 54(19): 4218-4228.
[15] WANG ShanShan,ZHAO ChenHui,LI HongLian,ZHANG BingBing,LIANG YingHai,SONG HongWei. Analysis of Fruit Aromatic Components of Ten Plum Germplasm Resources in Northeast China [J]. Scientia Agricultura Sinica, 2021, 54(11): 2476-2486.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!