Scientia Agricultura Sinica ›› 2022, Vol. 55 ›› Issue (24): 4823-4839.doi: 10.3864/j.issn.0578-1752.2022.24.004

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY • Previous Articles     Next Articles

SPAD Value Inversion of Cotton Leaves Based on Satellite-UAV Spectral Fusion

WANG ShuTing1(),KONG YuGuang2,ZHANG Zan3,CHEN HongYan1(),LIU Peng4   

  1. 1National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources/College of Resources and Environment, Shandong Agricultural University, Taian 271018, Shandong
    2Shandong Institute of Territorial and Spatial Planning, Ji’nan 250014
    3Lunan High Speed Railway Co., Ltd, Ji’nan 250098
    4College of Agronomy, Shandong Agricultural University, Taian 271018, Shandong
  • Received:2022-01-17 Accepted:2022-06-06 Online:2022-12-16 Published:2023-01-04
  • Contact: HongYan CHEN E-mail:wstwang@163.com;chenhy@sdau.edu.cn

Abstract:

【Objective】The aim of this study was to improve the inversion accuracy of chlorophyll content in cotton leaves, and to grasp its spatial distribution characteristics in Xiajin county, Shandong province. 【Method】Taking Xiajin county, Dezhou city, Shandong province as the study area and Dalizhuang cotton field in Xiajin county as the test area, the relative value of chlorophyll content (SPAD value) in cotton leaves in the experimental area was measured by SPAD (soil and plant analyzer development), and obtained the near earth multispectral image of unmanned aerial vehicle (UAV) and Sentinel-2A MSI (MSI) satellite image in the study area in the same period; Then, based on the spectral reflectance of UAV and MSI satellite images, the optimal spectral parameters were constructed and selected, and the inversion model of SPAD value was established by multiple linear regression (MLR); Finally, the quadratic polynomial fitting method was used to fuse the optimal spectral parameters corresponding to UAV and Sentinel-2A MSI. By comparing and analyzing the model effects before and after fusion, the inversion model was optimized, and the SPAD value inversion of the study area was realized. 【Result】(REG-R)/(REG+R), R/G, Cl(red edge) and NDVI could be the optimal spectral parameters of SPAD value. The precision of cotton leaf SPAD inversion model based on UAV near ground image was better than that based on satellite image; After quadratic polynomial fitting, the calibration R2 was increased by 0.015-0.057, and RMSE was decreased by 0.457-0.638, while the validation R2 was increased by 0.040-0.085, RMSE was decreased by 0.387-0.397, and RPD was increased by 0.020-0.139. The fused spectral parameters based on Sentinel-2A MSI image were input to the inversion model based on UAV data (Fused MSI-ModUAV), the high inversion accuracy of SPAD value in cotton leaves could be obtained, with the model calibration R2 up to 0.672, RMSE of 3.982, validation R2 up to 0.713, RMSE of 3.859, and RPD of 1.685. Based on the above model, two inversion prediction maps of different scales were obtained. The SPAD value of cotton leaves in the test area showed the distribution trend of high in the south and low in the north, and the study area showed the distribution trend of low in the middle and high around, which were consistent with the field situation and showed the model had a good prediction effect. 【Conclusion】Therefore, the fusion of UAV and satellite image data by using quadratic polynomial fitting method could better realize the quantitative inversion of regional high-precision crop growth indicators. The research results could enrich the theory and technology of multi-source remote sensing fusion, and provide the technical reference and data support for cotton growth monitoring and precision production.

Key words: SPAD value, UAV, Sentinel-2A MSI, inversion model, quadratic polynomial fitting method

Fig. 1

Location of the study area and test area (a: Shandong province; b: Study area; c: Test area)"

Table 1

Corresponding relationship between UAV and Sentinal-2A MSI bands"

波段名称
Band name
无人机 UAV 卫星 Sentinel-2A MSI
波段
Band
中心波长
Central wavelength (nm)
带宽
Bandwidth (nm)
波段
Band
中心波长
Central wavelength (nm)
带宽
Bandwidth (nm)
G Green 550 40 B3-Green 560 45
R Red 660 40 B4-Red 665 38
REG Red Edge 735 10 B6-Vegetation Red Edge 740 18
NIR Near IR 790 40 B7- Vegetation Red Edge 783 28

Table 2

Spectral parameters and calculation formulas"

植被指数
Vegetation index
名称
Name
计算公式
Calculation formula
参考文献
Reference
RVI 比值植被指数 Ratio vegetation index NIR/R [22]
DVI 差值植被指数 Difference vegetation index NIR-R
GNDVI 绿色归一化植被指数 Green normalized difference vegetation index (NIR-G)/(NIR+R)
NDRE 红边植被指数 Normalized difference red edge (NIR-REG)/(NIR+REG) [29]
CL(red edge) 红边叶绿素指数Ⅱ Red edge chlorophyII index REG/R-1 [31]
MCARI 改进叶绿素吸收比值指数 Modified chlorophyII absorption ratio index [(REG-R)-0.2×(REG-G)] ×(REG/R)
TCARI 转化叶绿素吸收比值指数 Transformed chlorophyII absorption ratio index 3×[(REG-R)-0.2×(REG-G)×(REG/R)]
OSAVI 优化型土壤调节植被指数 Optimized soil adjusted vegetation index 1.16×(NIR-R)/(NIR+R+0.16)
NDVI 归一化植被指数 Normalized difference vegetation index (NIR-R)/(NIR+R)
CIRE 红边叶绿素指数Ⅰ Red edge chlorophyII index NIR/REG-1 [36]
MSR 改进简单比值植被指数 Modified simple ratio (NIR/R-1)(NIR/R+1)0.5 [37]
RDVI 重归一化植被指数 Renormalized difference vegetation index (NIR-R)/(NIR+R)0.5
(REG-R)/(REG+R) (REG-R)/(REG+R) [38]
R/G R/G
sqrt(R2+G^2) sqrt(R^2+G^2)
G×R G×R
G×R×REG G×R×REG
REG-R REG-R
REG-NIR REG-NIR

Fig. 2

Technology roadmap"

Table 3

Statistical analysis of cotton leave SPAD value"

样本类型
Sample type
样本数
Number of samples
最大值
Maximum value
最小值
Minimum value
平均值
Average value
标准差
Standard deviation
建模集 Calibration set 64 59.5 20.2 43.5 7.8
验证集 Validation set 31 68.2 17.1 43.3 8.3
全部 All the samples 95 68.2 17.1 43.3 8.6
研究区 Study area 58 63.1 21.5 46.9 8.1

Table 4

Correlation analysis between spectral parameters and measured SPAD values of samples"

序号
Number
光谱参量
Spectral parameter
相关系数 Correlation coefficient
无人机 UAV 卫星 Sentinel-2A MSI
1 NDVI 0.688** 0.559**
2 RVI 0.655** 0.518*
3 DVI 0.300 0.558*
4 MSR 0.683** 0.534
5 RDVI 0.530* 0.553*
6 GNDVI -0.613 0.542
7 CIRE -0.547* 0.411
8 OSAVI 0.531* 0.545*
9 CL(red edge) 0.756** 0.549*
10 MCARI 0.687** 0.517*
11 TCARI -0.490 -0.496
12 NDRE -0.196 0.412
13 (REG-R)/(REG+R) 0.800** 0.557**
14 R/G -0.768** -0.547**
15 sqrt(R^2+G^2) -0.653** -0.529*
16 G×R -0.626** -0.541*
17 G×R×REG -0.405 -0.510
18 REG-R 0.680** 0.548**
19 REG-NIR 0.023 -0.505*

Table 5

Inversion models of cotton leaf SPAD value"

数据源
Data source
反演模型
Inversion model
建模精度
Calibration accuracy
验证精度
Verification accuracy
决定系数
R2
均方根误差RMSE 决定系数
R2
均方根误差RMSE 相对分析误差
RPD
无人机
UAV
Y=37.362+1.093×NDVI+1.26×CL(red edge)- 4.946×R/G+8.286×(REG-R)/(REG+R) 0.709 3.782 0.753 3.589 2.045
卫星
Sentinel-2A MSI
Y=-39.914+152.318×NDVI-2.267×CL(red edge)- 4.990×R/G-25.234×(REG-R)/(REG+R) 0.452 5.823 0.447 5.909 1.521

Fig. 3

Scatter plot of samples’ SPAD value in the test area"

Fig. 4

Distribution map of cotton-growing area in the study area"

Table 6

Confusion matrix of extracted cotton-growing area"

区域
Region
棉花种植区
Cotton-growing area
非棉花种植区
Non cotton-growing area
样本总数
Total number of samples
棉花种植区 Cotton-growing area 1007 60 1067
非棉花种植区 Non cotton-growing area 12 1360 1372
样本总数 Total number of samples 1019 1420 2439

Table 7

Characteristic spectral parameters before and after fusion"

最优光谱参量
Optimal spectral parameter
光谱转换公式
Spectral conversion formula
融合前 Unfused 融合后 Fused
拟合度R2
Fitting
degree
相关系数
Correlation coefficient
拟合度R2
Fitting degree
相关系数
Correlation coefficient
NDVI NDVI′= 0.325×NDVI2+0.2682×NDVI+0.4053 0.704 0.559** 0.721 0.580**
CL(red edge) CL(red edge)′=-0.2687×CL(red edge)2+3.0079×CL
(red edge) -0.821
0.670 0.549** 0.714 0.611**
R/G R/G′=-0.0682×R/G2+0.4316×R/G+0.3128 0.697 -0.547** 0.712 -0.587**
(REG-R)/(REG+R) (REG-R)/(REG+R)′= 0.4694×(REG-R)/(REG+R)2+
0.1082×(REG-R)/(REG+R)+0.453
0.695 0.557** 0.718 0.618**

Table 8

Inversion models of cotton leaf SPAD value in the study area"

模型
Model
公式
Formula
建模精度
Calibration accuracy
验证精度
Verification accuracy
决定系数
R2
均方根误差RMSE 决定系数
R2
均方根误差RMSE 相对分析误差
RPD
MSI-ModMSI Y=-39.914+152.318×NDVI-2.267×CL(red edge)- 4.990×R/G-25.234×(REG-R)/(REG+R) 0.452 5.823 0.447 5.909 1.521
Fused MSI-ModMSI Y=-39.914+152.318×NDVI′-2.267×CL(red edge)′- 4.990×R/G′-25.234×(REG-R)/(REG+R)′ 0.467 5.366 0.487 5.522 1.541
MSI-ModUAV Y=37.362+1.093×NDVI+1.26×CL(red edge)- 4.946×R/G+8.286×(REG-R)/(REG+R) 0.615 4.620 0.628 4.256 1.546
Fused MSI-ModUAV Y=37.362+1.093×NDVI′+1.26×CL(red edge)′- 4.946×R/G′+8.286×(REG-R)/(REG+R)′ 0.672 3.982 0.713 3.859 1.685
AR Fused MSI-ModUAV Y=37.362+1.066×NDVI+5.388×CL(red edge)- 3.645×R/G+18.652×(REG-R)/(REG+R) 0.638 4.259 0.664 4.015 1.598

Fig. 5

Scatter plot of samples’ SPAD value in the study area"

Fig. 6

Inversion map of cotton leaf SPAD value in the test area"

Table 9

Classification statistics of cotton leaf SPAD value in the test area"

等级
Level
反演值 Inversion value 样点实测值 Measured value of sample point 样点预测值 Predicted values of sample points
像元数
Number of pixels
所占比例
Proportion (%)
个数
Number
所占比例
Proportion (%)
个数
Number
所占比例
Proportion (%)
<30 664632 6.747 6 6.316 4 4.210
30-40 1829536 18.574 17 17.895 17 17.895
40-50 6483804 65.825 55 57.895 62 65.263
>50 872169 8.854 17 17.894 12 12.632

Fig. 7

Inversion map of cotton leaf SPAD value in the study area"

Table 10

Classification statistics of cotton leaf SPAD value in the study area"

等级
Level
反演图 Inversion map
像元数
Number of pixels
所占比例
Proportion (%)
<30 15749 1.395
30-40 186849 16.553
40-50 492603 43.640
>50 433588 38.412
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