Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (20): 4149-4155.doi: 10.3864/j.issn.0578-1752.2012.20.004

• PLANT PROTECTION • Previous Articles     Next Articles

HgCl2-Induced Programmed Cell Death in Tobacco Cells

 LIU  Jin-Wei, JIAO  Jiao, WANG  Lei, WANG  Lian-Lian, CHU  Xiao-Jing, LIANG  Yuan-Cun   

  1. 1.山东农业大学植物保护学院，山东泰安 271018
  • Received:2012-03-09 Online:2012-10-15 Published:2012-04-26

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Abstract: 【Objective】 The objective of this study is to investigate the characteristics of cell death and molecular mechanisms induced by HgCl2. 【Method】 HgCl2-induced cell death was studied in suspension-cultured tobacco cells via neutral red staining, a set of cellular morphological and biochemical characteristics was elucidated in the occurrence of cell death, and pharmacological method was used to explore the mechanisms activated by HgCl2. 【Result】 Within 24 and 48 h, HgCl2 treatment induced cell death in a concentration-dependent manner. Tobacco cells treated with HgCl2 showed typical features of PCD such as chromatin condensation, TUNEL-positive nuclei and DNA ladder formation. HgCl2-induced cell death was accompanied by an increasing production of H2O2. Consistently, the addition of the antioxidants greatly reduced cell death. Pretreatment with protein kinase inhibitor staurosporin, phospholipase C (PLC) inhibitors U73122 and neomycin, and phospholipase D (PLD) inhibitor 1-butanol significantly diminished HgCl2-induced cell death and H2O2 accumulation. 【Conclusion】 HgCl2 induces PCD exhibiting apoptotic-like features. The cell death process requires increased H2O2 production regulated by activation of protein phosphorylation and phospholipid signaling pathways.

Key words: tobacco, HgCl2, programmed cell death

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