Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (5): 877-884.doi: 10.3864/j.issn.0578-1752.2012.05.007

• PLANT PROTECTION • Previous Articles     Next Articles

mRNA Expression and Physiological Function of Chitin Synthase1 from Oxya chinensis (Thunberg)

 YU  Zhi-Tao, LIU  Xiao-Jian, MA  恩Bo, GUO  Ya-Ping, ZHANG  Jian-Zhen   

  1. 1.山西大学应用生物学研究所，太原 030006
  • Received:2011-06-20 Online:2012-03-01 Published:2011-10-02

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Abstract: 【Objective】The objective of this study is to analyze the mRNA expression characteristics and physiological function of chitin synthase1 gene (OcCHS1) from the Chinese rice grasshopper, Oxya chinensis (Thunberg), and to provide a theoretical basis for effective pests control based on RNAi.【Method】Based on the known partial conservative cDNA fragment of OcCHS1 (GenBank accession number: HM214491), primers were designed for mRNA expression analysis. The developmental and tissue-specific expression patterns of OcCHS1 were determined by RT-PCR and qPCR. RNAi experiment was performed to explore the physiological function. 【Result】 OcCHS1 was expressed at all the developmental stages. Tissue-specific analysis showed that OcCHS1 was highly expressed in the integument, followed in the trachea, and lowest in other tissues. RNAi-based gene silencing experiment showed that the fourth day of the 4th instar nymphs injected by OcCHS1-speci?c dsRNA could repress the transcript levels of OcCHS1 by 70.8% compared to those in the control nymphs. Moreover, dsRNA injections signi?cantly increased abnormalities, such as stunted development, uncompleted molting, crimpy abdomen and eventually died. Consequentially, 85.2% of mortalities were observed, while the mortality in the control nymphs was only 7.4%. 【Conclusion】OcCHS1 is essential for development and mainly responsible for chitin synthesis in cuticle and trachea of O. chinensis. Moreover, silencing of chitin synthase 1 gene by RNAi is lethal to O. chinensis.

Key words: Oxya chinensis (Thunberg), chitin synthase 1 gene, mRNA expression, RNA interference

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