Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (4): 675-684.doi: 10.3864/j.issn.0578-1752.2012.04.008

• PLANT PROTECTION • Previous Articles     Next Articles

Impact of Spraying Glyphosate on Growth and Yield Component of Glyphosate-Tolerant Soybean at Different Growth Stages

 LIU  Wen-Juan, LIU  Yong, HUANG  Xiao-Qin, ZHOU  Xi-Quan, SONG  Jun, YIN  Quan, WANG  Dong, TAO  Li, ZHANG  Fu-Li, CHANG  Li-Juan, ZHANG  Lei, LEI  Shao-Rong   

  1. 1.四川省农业科学院分析测试中心，成都 610066
    2.四川省农业科学院植物保护所，成都 610066
  • Received:2011-06-03 Online:2012-02-15 Published:2011-11-30

PDF

1161

Cited

3

Abstract: 【Objective】 The objective of this study is to research the impact of glyphosate on growth and fecundity of glyphosate-tolerant soybean and to provide statistics and a theoretical basis of time and concentration for weeding control by glyphosate.【Method】A random complete block design was used and the influence of spraying 41% glyphosate isopropylammonium AS at different rates on growth and yield component of transgenic soybean cultivar GTS-40-3-2 at V1-V5 stages was studied in the field. Also, the effect of glyphosate on weeding control was investigated. 【Result】 It was found that glyphosate was effective at 1.23-12.30 kg•ai•hm-2 to control weed. However, the glyphosate more than recommended rate of 1.23-2.46 kg•ai•hm-2 depressed the number of stem nods and compound leaves, and reduced the number of seeds and seeds weight per plant of GTS-40-3-2. There were no obviously negative influence of spraying glyphosate on number of effective pods per plant and 100-seed-weight of GTS-40-3-2, and the main agronomic traits related to yield including plant height, pod height and number of effective embranchments even increased after dealing with glyphosate at lower concentration. There were different effects of spraying glyphosate on growth and fecundity of GTS-40-3-2 at different growth stages. Glyphosate of 4.92-7.38 kg•ai•hm-2 restrained distinctly soybean growth and yield on V1 and V2 stages, but did not injure yield component at V3-V5 stages. The stem nodes and leaves of GTS-40-3-2 were decreased in 10-20 days after treatment (DAT) with glyphosate at V3-V5 stages, then returned to control in 30 d. Glyphosate at 9.84-12.30 kg•ai•hm-2 reduced the number of seeds and seed weight per plant of GTS-40-3-2 at V4 and V5 stages, but did not affect them at V3 stage.【Conclusion】 Spraying glyphosate at 1.23-2.46 kg•ai•hm-2 not only was available for weeding control but also maintained the growth and yield component of GTS-40-3-2, which could be safe to use at V1-V5 growth stages of the glyphosate-tolerant soybean. Application of gyphosate beyond 1.23-2.46 kg•ai•hm-2 depressed the seed number but did not affect the seed mass of GTS-40-3-2. There were differences in growth and yield component depression by glyphosate between growth stages of GTS-40-3-2. Soybean at V1 and V2 stages was more sensitive to glyphosate than V3-V5. It was most safe to spray glyphosate at V3 stage compared with other stages in consideration of soybean growth and fecundity.         

Key words: glyphosate-tolerant soybean, glyphosate isopropylammonium AS, growth, yield component

[1]Phillip A C. RAPD analysis of seed purity in a commercial hybrid cabbage (Brassica oleraca var. Capital) cultivar. Genome, 2000, 43(2): 317-321.

[2]Schönbrunn E, Eschenburg S, Shuttleworth W A, Schloss J V, Amrhein N, Evans J N S, Kabsch W. Interaction of the herbicide glyphosate with its target enzyme 5-enolpyruvylshikimate 3-phosphate synthase in atomic detail. Proceedings of the National Academy of Sciences, 2001, 98(4): 1376-1380.

[3]Dill G M. Glyphosate-resistant crops: history, status and future. Pest Management Science, 2005, 61(3): 219-224.

[4]Roberts R K, Pendergrass R, Hayes R M. Economic analysis of alternative herbicide regimes on roundup ready soybeans. Journal of Production Agriculture, 1999, 12(3): 449-454. 

[5]Webster E P, Bryant K J, Earnest L D. Weed control and economics in nontransgenic and glyphosate-resistant soybean (Glycine max). Weed Technology, 1999, 13(3): 586-593.

[6]Reddy K N. Glyphosate-resistant soybean as a weed management tool: opportunities and challenges. Weed Biology and Management, 2001, 1(4): 193-202. 

[7]Heatherly L G, Elmore C D, Spurlock S R. Weed management systems for conventional and glyphosate-resistant soybean with and without irrigation. Agronomy Journal, 2002, 94(6): 1419-1428. 

[8]Heatherly L G, Spurlock S R, Reddy K N. Weed management in nonirrigated glyphosate-resistant and non-resistant soybean following deep and shallow fall tillage. Agronomy Journal, 2004, 96(3): 742-749.

[9]Shaner D L. Role of translocation as a mechanism of resistance to glyphesate. Weed Science, 2009, 57(1): 118-123.

[10]沈晓峰, 栾凤侠, 陶  波. 抗草甘膦转基因大豆生物与环境安全 性. 东北农业大学学报, 2007, 38(3): 401-404.

Shen X F, Luan F X, Tao B. Biosafety and environmental safety of genetic modified soybean resistant to glyphosate. Journal of Northeast Agricultural University, 2007, 38(3): 401-404. (in Chinese)

[11]Reddy K N, Hoagland R E, Zablotowicz R M. Effect of glyphosate on growth, chlorophyll, and nodulation in glyphosate-resistant and susceptible soybean (Glycine max) varieties. Journal of New Seeds, 2001, 2(3): 37-52.  

[12]Reddy K N, Rimando A M, Duke S O. Aminomethylphosphonic acid, a metabolite of glyphosate, causes injury in glyphosate-treated, glyphosate-resistant soybean. Journal of Agricultural and Food Chemistry, 2004, 52(16): 5139-5143.

[13]卜贵军, 刘洪梅, 李  英, 崔  琳, 王学东. 草甘膦对大豆超微结构及光合指标影响的研究. 电子显微学报, 2008, 27(4): 322-330.

Bu G J, Liu H M, Li Y, Cui L, Wang X D. Soybean ultrastructure and photosynthesis index as affected by glyphosate. Journal of Chinese Electron Microscopy Society, 2008, 27(4): 322-330. (in Chinese)

[14]Zobiole L H S, Oliveira Jr R S, Visentainer J V, Kremer R J, Bellaloui N, Yamada T. Glyphosate affects seed composition in glyphosate- resistant soybean. Journal of Agricultural and Food Chemistry, 2010, 58(7): 4517-4522.

[15]Zablotowicz R M, Reddy K N. Nitrogenase activity, nitrogen content, and yield responses to glyphosate in glyphosate-resistant soybean. Crop Protection, 2007, 26(3): 370-376.

[16]Krausz R F, Young B G. Response of glyphosate-resistant soybean (Glycine max) to trimethylsulfonium and isopropylamine salts of glyphosate. Weed Technology, 2001, 15(4): 745-749. 

[17]Reddy K N, Zablotowicz R M. Glyphosate-resistant soybean response to various salts of glyphosate and glyphosate accumulation in soybean nodules. Weed Science, 2003, 51(4): 496-502.

[18]Elmore R W, Roeth F W, Klein R N, Knezevic S Z, Martin A, Nelson L A, Shapiro C A. Glyphosate-resistant soybean cultivar response to glyphosate. Agronomy Journal, 2001, 93(2): 404-407.

[19]Benbrook C. Evidence of the magnitude and consequences of the Roundup Ready soybean yield drag from university-based varietal trials in 1998. Ag BioTech InfoNet Technical Paper Number 1, 1999.

[20]Elmore R W, Roeth F W, Nelson L A, Shapiro C A, Klein R        N, Knezevic S Z, Martin A. Glyphosate-resistant soybean cultivar yields compared with sister lines. Agronomy Journal, 2001, 93(2): 408-412.

[21]Nelson K A, Renner K A. Soybean growth and development as affected by glyphosate and postemergence herbicide tank mixtures. Agronomy Journal, 2001, 93(2): 428-434.

[22]Young B G, Young J M, Matthews J L, Owen M D K, Zelaya L A, Hartzler R G, Wax L M, Rorem K W, Bollero G A. Soybean development and yield as affected by three postemergence herbicides. Agronomy Journal, 2003, 95(5): 1152-1156.

[23]Lamb D C, Kelly D E, Hanley S Z, Mehmood Z, Kelly S L. Glyphosate is an inhibitor of plant cytochrome P450: functional expression of Thlaspi arvensae cytochrome P45071B1/reductase fusion protein in Escherichia coli. Biochemical and Biophysical Research Communications, 1998, 244(1): 110-114.

[24]King C A, Purcell L C, Vories E D. Plant growth and nitrogenase activity of glyphosate-tolerant soybean in response to foliar glyphosate applications. Agronomy Journal, 2001, 93(1): 179-186.

[25]原向阳, 郭平毅, 张丽光, 王  鑫, 姚满生, 王宏富. 第三复叶期喷施草甘膦对转基因大豆和普通大豆生理指标的影响. 中国农业科学, 2008, 41(11): 3886-3892.

Yuan X Y, Guo P Y, Zhang L G, Wang X, Yao M S, Wang H F. Impact of glyphosate on physiological index of transgenic and conventional soybean on three-trifoliolate leaf stage. Scientia Agricultura Sinica, 2008, 41(11): 3886-3892. (in Chinese)

[26]原向阳, 郭平毅, 张丽光, 王  鑫, 赵  锐, 姚满生, 王宏富. 不同时期喷施草甘膦对大豆生理指标的影响. 中山大学学报: 自然科学版, 2009, 48(2): 90-94.

Yuan X Y, Guo P Y, Zhang L G, Wang X, Zhao R, Yao M S, Wang H F. Impact of spraying glyphosate on physiological index of soybean at different growth stage. Acta Scientiarum Naturalun Universitatis Sunyatseni, 2009, 48(2): 90-94. (in Chinese)
[1] WANG ShaoHua, FAN QiuLi, YANG JinChang, SUN YuJie, YU Niu, JIANG ShouQun. Effects of Different Levels of Mytilaria laosensis Leaves Feeding on Growth Performance, Immune Function, Antioxidant Capacity, Carcass Quality and Meat Quality of Yellow-Feathered Chickens [J]. Scientia Agricultura Sinica, 2026, 59(5): 1111-1127.
[2] LIU HaiQing, JIN JiaoJiao, SUN WanCang, CHAI Peng, QI WeiLiang, YANG Gang, LI Chan, LUO XueMei, SU YunYun, QIN XueXue. Morphogenesis of the Low-Growth Point and Its Multi-Hormonal Regulatory Mechanism During Overwintering in Winter Rapeseed (Brassica napus L.) [J]. Scientia Agricultura Sinica, 2026, 59(5): 951-966.
[3] XIAO ChangChun, WEI XinYu, ZENG YueHui, HUANG JianHong, XU XuMing. Accumulation Characteristics of Anthocyanins in Black Rice Under Different Sowing Dates and Its Relationship with Meteorological Factors [J]. Scientia Agricultura Sinica, 2025, 58(5): 890-906.
[4] QIU HaiLong, LI Pan, ZHANG DianKai, FAN ZhiLong, HU FaLong, CHEN GuiPing, FAN Hong, HE Wei, YIN Wen, ZHAO LianHao. Compensatory Effects of Multiple Cropping Green Manure on Growth and Yield Loss of Nitrogen-Reduced Spring Wheat in Oasis Irrigation Areas of Northwest China [J]. Scientia Agricultura Sinica, 2025, 58(3): 443-459.
[5] GAO ChunHua, ZHAO HaiJun, ZHAO FengTao, KONG WeiLin, JU FeiYan, LI ZongXin, SHI DeYang, LIU Ping. Effect of Growth Regulators on the Stem Characteristics and Yield of Summer Maize in Maize-Soybean Strip Intercropping [J]. Scientia Agricultura Sinica, 2025, 58(23): 4920-4935.
[6] YANG Fu, WEI ShanJun, ZHANG XiaoXia, ZHANG LinMin, LIU HongXiu, LI YiJun, TONG YaPing. Characterization and Utilization of Rhizosphere Microbiota from Wild Plants in Lalu Wetland in Xizang for Alleviating Saline-Alkali Stress in Maize [J]. Scientia Agricultura Sinica, 2025, 58(20): 4144-4157.
[7] WANG Di, HAN ShouAn, ZHANG Wen, WANG Min, SHI HuiDong, ZHU XueHui, BAI ShiJian, LIU XuPeng, TIAN Jia, XIE Hui. Effects of Different Plant Growth Regulators on Fruit and Raisin Quality of Thompson Seedless Grapes [J]. Scientia Agricultura Sinica, 2025, 58(18): 3767-3782.
[8] HU JiaYan, SHEN ZhiHan, WEN LiHui, YU JiaHao, ZHANG YuJun, JIANG DongHua. Identification and Evaluation of Biocontrol Actinomycetes Against Xanthomonas oryzae pv. oryzicola for Disease Suppression and Growth Promotion in Rice [J]. Scientia Agricultura Sinica, 2025, 58(17): 3434-3450.
[9] XU JiaXin, HUA Nan, WANG YongQiang, XU Hao, LIU Zhen, ZHAO XiaoRui, LI Yue, CHEN QiWei, YE Lin. Response Surface Methodology Optimization of Water, Fertilizer, and Pesticide Coupling on Chili Pepper Growth, Photosynthetic Characteristics, and Root Rot [J]. Scientia Agricultura Sinica, 2025, 58(14): 2869-2884.
[10] ZHAO Yong, ZHANG ZhongFu, WANG YuTong, AI Jing, LIU JiaYong, WU JianMing, DENG Jun, ZHANG YueBin. Effects of Potassium Application on Root Rhizosphere Microbial Community Changes and Growth of Sugarcane [J]. Scientia Agricultura Sinica, 2025, 58(13): 2630-2644.
[11] LI Hao, TIAN YuYang, ZHANG ZiMing, CAO YiFan, CIREN LuoBu, NIMA CangJue, DANZENG LuoSang, LEI ChuZhao, BASANG ZhuZha, CHEN NingBo. Research Progress on the Function of Bovine Thyroid Gene and Its Correlation with Environmental Adaptability [J]. Scientia Agricultura Sinica, 2025, 58(13): 2682-2692.
[12] DONG Ming, QI Hong, ZHANG Qian, WANG Yan, WANG ShuLin, FENG GuoYi, LIANG QingLong, GUO BaoSheng. The Impact of Sowing Methods on the Seed Germination Environment and Cotton Emergence and Growth [J]. Scientia Agricultura Sinica, 2025, 58(12): 2346-2357.
[13] WU YongBao, TANG Jing, CAO JunTing, WANG QiMeng, XIE Ming, ZHOU ZhengKui, HOU ShuiSheng, WEN ZhiGuo. Effects of Methionine Supplementation on Growth Performance, Carcass Trait, and Plasma Biochemical Indice of Growing Pekin Ducks Fed a Low-Energy and Low-Protein Diet [J]. Scientia Agricultura Sinica, 2025, 58(12): 2475-2486.
[14] CHEN HuiXian, HE Wen, RUAN LiXia, HUANG XiaoJuan, LAN Xiu, CAI ZhaoQin, LI HengRui, HUANG ZhenLing, WEI WanLing, LIANG ZhenHua, LI TianYuan, CAO Sheng, LI XiDi, WEI JunCan. Effects of Intercropping Soybean on Cassava Growth and Soil Characteristics [J]. Scientia Agricultura Sinica, 2025, 58(11): 2176-2189.
[15] ZHANG YuZhou, WANG YiZhao, GAO RuXi, LIU YiFan. Research Progress on Root System Architecture and Drought Resistance in Wheat [J]. Scientia Agricultura Sinica, 2024, 57(9): 1633-1645.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd