Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (1): 102-110.doi: 10.3864/j.issn.0578-1752.2012.01.012

• HORTICULTURE • Previous Articles     Next Articles

Cloning and Expression of the Sorbitol Dehydrogenase Gene Family in Apple

 LIANG  Dong, WU  Shan, WANG  Su-Fang, MA  Feng-Wang   

  1. 1.西北农林科技大学园艺学院/旱区作物逆境生物学国家重点实验室，陕西杨凌 712100
  • Received:2011-06-30 Online:2012-01-01 Published:2011-11-02

PDF

867

Cited

23

Abstract: 【Objective】 The objective of this experiment was to further investigate the molecular characteristics of sorbitol dehydrogenase (SDH) in apple. 【Method】 The cDNAs and gDNAs of SDH gene family were isolated from the fruit of Malus domestica cv. Gala, and the mRNA expression levels of SDH gene family during leaf and fruit development were studied. 【Result】Twelve cDNAs of SDH were cloned from apple fruit, in turn being named MdSDH7-MdSDH18. Intron/exon structure analysis indicated that MdSDH18 contained 6 extons and 5 introns, whereas others contained 2 extons and 1 intron. The mRNA expression levels and enzyme activities of SDH were detected during leaf and fruit developments. According to the amino acid sequence homology, these SDHs were divided into two groups, A and B. During the fruit development, the mRNA expression levels of all SDHs at middle phase and late phase were higher than that of early phase, however, the changes of SDH enzyme activity were opposite to gene expression patterns. During leaf development, mRNA expression levels of A-SDH were in the order of young leaf ＞ senescence leaf ＞ mature leaf, with activities showing similar patterns to gene expression. However, mRNA expression   levels of B-SDH was in the order of senescence leaf ＞mature leaf ＞ young leaf. 【Conclusion】 These results further suggest that SDH is a gene family in apple, and the expression of gene family members shows different patterns during leaf and fruit developments.

Key words: apple, sorbitol dehydrogenase, gene family, gene expression

[1]Bieleski R L. Sugar alcohols//Loewus F A, Tanner W. Plant Carbohydrates I: Inrtacellular Carbohydrates. New York: Springer Verlag: 1982: 158-192.

[2]Loescher W H. Physiology and metabolism of sugar alcohols in higher plant. Physiologia Plantarum, 1987, 70(3):553-557.

[3]Zhou R, Cheng L L, Wayne R. Purification and characterization of sorbitol-6-phosphate phosphatase from apple leaves. Plant Science, 2003, 165:227-232.

[4]Lo Biancoa R, Riegera M, Sung S S. Effect of drought on sorbitol and sucrose metabolism in sinks and sources of peach. Physiologia Plantarum, 2000, 108: 71-78.

[5]Kobashi K, Gemma H, Iwahori S. Abscisic acid content and sugar metabolism of peaches grown under water stress. Journal of the American Society for Horticultural Science, 2000, 125: 425-428.

[6]Zhang J, Yao Y, Streeter J G, Ferree D C. Influence of soil drought stress on photosynthesis, carbohydrates and the nitrogen and phophorus absorb in different section of leaves and stem of Fugi/M.9EML, a young apple seedling. African Journal of Biotechnology, 2010, 9(33): 5320-5325.

[7]Kanayama Y, Watanabe M, Moriguchi R, Deguchi M, Kanahama K, Yamaki S. Effects of low temperature and abscisic acid on the expression of the sorbitol-6-phosphate dehydrogenase gene in apple leaves. Journal of the Japanese Society for Horticultural Science, 2006, 75(1): 20-25.

[8]Suleman P, Steiner P W. Relationship between sorbitol and solute potential in apple shoots relative to fire blight symptom development after infection by Erwinia amylovora. Phytopathology, 1994, 84(10): 1244-1250.

[9]Yamaki S, Ishikawa K. Roles of four sorbitol related enzymes and invertase in the seasonal alleration of sugar metabolism in apple tissue. Journal of the American Society for Horticultural Science, 1986, 111(1):134-137.

[10]Yamada K, Oura Y, Mori H, Yamaki S. Cloning of NAD-dependent sorbitol dehydrogenase from apple fruit and gene expression. Plant & Cell Physiology, 1998, 39(12):1375-1379.

[11]Park S W, Song K J, Kim M Y, Hwang J H, Shin Y U, Kim W C, Chung W I. Molecular cloning and characterization of four cDNAs encoding the isoforms of NAD-dependent sorbitol dehydrogenase from the Fuji apple. Plant Science, 2002, 162:513-519.

[12]Nosarszewski M, Clements A M, Downie B A, Archbold D D. Sorbitol dehydrogenase expression and activity during apple fruit set and early development. Physiologia Plantarum, 2004, 121:391-398.

[13]Nosarszewski M, Archbold D D. Tissue-specific expression of SORBITOL DEHYDROGENASE in apple fruit during early development. Journal of Experimental Botany, 2007, 58:1863-1872.

[14]Wang X L, Xu Y H, Peng C C, Fan R C，Gao X Q. Ubiquitous distribution and different subcellular localization of sorbitol dehydrogenase in fruit and leaf of apple. Journal of Experimental Botany, 2009, 60(3): 1025-1034.

[15]Bantog N A, Yamada K, Niwa N, Shiratake K, Yamaki S. Gene expression of NAD+-dependent sorbitol dehydrogenase and NADP- dependent sorbitol-6-phosphate dehydrogenase during development of loquet fruit. Journal of the Japanese Society for Horticultural Science, 2000, 69(3):231-236.

[16]Yamada K, Niwa N, Shiratake K, Yamaki S. cDNA cloning of NAD-dependent sorbitol dehydrogenase from peach fruit and its expression during fruit development. Journal of Horticulture Science & Biotechnology, 2001, 76(5): 581-587.

[17]Duangsrisai S, Yamada K, Bantog N A, Shiratake K, Kanayama Y, Yamaki S. Presence and expression of NAD+-dependent sorbitol dehydrogenase and sorbitol-6-phosphate dehydrogenase genes in strawberry. Journal of Horticulture Science & Biotechnology, 2007, 82(2): 191-198.

[18]Ito A, Hayama H, Kashimura Y. Partial cloning and expression analysis of genes encoding NAD+-dependent sorbitol dehydrogenase in pear bud during flower bud formation. Scientia Horticulturae, 2005, 103(4):413-420.

[19]Kim H Y, Ahn J C, Choi J H, Hwang B, Choi D W. Expression and cloning of the full-length cDNA for sorbitol-6-phosphate dehydrogenase and NAD-dependent sorbitol dehydrogenase from pear (Pyrus pyrifolia N.). Scientia Horticulturae, 2007, 112(4): 406-412.

[20]Chang S, Puryear J, Cairney J. A simple and efficient method for isolating RNA from pine trees. Plant Molecular Biology Reporter, 1993, 11:113-116.

[21]王关林, 方宏筠. 植物基因工程. 第二版. 北京：科学出版社, 2002.

Wang G L, Fang H J. Plant Genetic Engineering. the second edition. Beijing: Science Press, 2002. (in Chinese)

[22]Teo G, Suzuki Y, Uratsu S L, Lampinen B, Ormonde N, Hu W K, DeJong T M, Dandekar A M. Silencing leaf sorbitol synthesis alters long-distance partitioning and apple fruit quality. Proceedings of the National Academy of Sciences of the USA, 2006, 103(49): 18842-18847.

[23]Lo Bianco, Rieger M, Sung S S. A simple, rapid extraction and assay procedure for NAD+-dependent sorbitol dehydrogenase in peach. Journal of the American Society for Horticultural Science, 1998, 123(6):1065-1068.

[24]Yamada K, Suzue Y, Hatano S, Tsukuda M, Kanayama Y, Shiratake K, Yamaki S. Chang in the activity and gene expression of sorbitol- and sucrose-related enzymes associated with development of ‘La France’ Pear fruit. Journal of the Japanese Society for Horticultural Science, 2006, 75(1): 38-44.

[25]Wu B H, Li S H, Nosarszewski M, Archbold D D. Sorbitol dehydrogenase gene expression and enzyme activity in apple: tissue specificity during bud development and response to rootstock vigor and growth manipulation. Journal of the American Society for Horticultural Science, 2010, 135(4):379-387.
[1] LIU RUI, ZHAO YuHan, FU ZhongJu, GU XinYi, WANG YanXia, JIN XueHui, YANG Ying, WU WeiHuai, ZHANG YaLing. Distribution and Variation of PWL Gene Family in Rice Magnaporthe oryzae from Heilongjiang Province and Hainan Province [J]. Scientia Agricultura Sinica, 2023, 56(2): 264-274.
[2] ZHANG KeKun,CHEN KeQin,LI WanPing,QIAO HaoRong,ZHANG JunXia,LIU FengZhi,FANG YuLin,WANG HaiBo. Effects of Irrigation Amount on Berry Development and Aroma Components Accumulation of Shine Muscat Grape in Root-Restricted Cultivation [J]. Scientia Agricultura Sinica, 2023, 56(1): 129-143.
[3] GU LiDan,LIU Yang,LI FangXiang,CHENG WeiNing. Cloning of Small Heat Shock Protein Gene Hsp21.9 in Sitodiplosis mosellana and Its Expression Characteristics During Diapause and Under Temperature Stresses [J]. Scientia Agricultura Sinica, 2023, 56(1): 79-89.
[4] DONG YongXin,WEI QiWei,HONG Hao,HUANG Ying,ZHAO YanXiao,FENG MingFeng,DOU DaoLong,XU Yi,TAO XiaoRong. Establishment of ALSV-Induced Gene Silencing in Chinese Soybean Cultivars [J]. Scientia Agricultura Sinica, 2022, 55(9): 1710-1722.
[5] CHEN XueSen, YIN HuaLin, WANG Nan, ZHANG Min, JIANG ShengHui, XU Juan, MAO ZhiQuan, ZHANG ZongYing, WANG ZhiGang, JIANG ZhaoTao, XU YueHua, LI JianMing. Interpretation of the Case of Bud Sports Selection to Promote the High-Quality and Efficient Development of the World’s Apple and Citrus Industry [J]. Scientia Agricultura Sinica, 2022, 55(4): 755-768.
[6] LU Xiang, GAO Yuan, WANG Kun, SUN SiMiao, LI LianWen, LI HaiFei, LI QingShan, FENG JianRong, WANG DaJiang. Analysis of Aroma Characteristics in Different Cultivated Apple Strains [J]. Scientia Agricultura Sinica, 2022, 55(3): 543-557.
[7] LAI ChunWang, ZHOU XiaoJuan, CHEN Yan, LIU MengYu, XUE XiaoDong, XIAO XueChen, LIN WenZhong, LAI ZhongXiong, LIN YuLing. Identification of Ethylene Synthesis Pathway Genes in Longan and Its Response to ACC Treatment [J]. Scientia Agricultura Sinica, 2022, 55(3): 558-574.
[8] SHU JingTing,SHAN YanJu,JI GaiGe,ZHANG Ming,TU YunJie,LIU YiFan,JU XiaoJun,SHENG ZhongWei,TANG YanFei,LI Hua,ZOU JianMin. Relationship Between Expression Levels of Guangxi Partridge Chicken m6A Methyltransferase Genes, Myofiber Types and Myogenic Differentiation [J]. Scientia Agricultura Sinica, 2022, 55(3): 589-601.
[9] GUO ShaoLei,XU JianLan,WANG XiaoJun,SU ZiWen,ZHANG BinBin,MA RuiJuan,YU MingLiang. Genome-Wide Identification and Expression Analysis of XTH Gene Family in Peach Fruit During Storage [J]. Scientia Agricultura Sinica, 2022, 55(23): 4702-4716.
[10] GAO XiaoQin,NIE JiYun,CHEN QiuSheng,HAN LingXi,LIU Lu,CHENG Yang,LIU MingYu. Geographical Origin Tracing of Fuji Apple Based on Mineral Element Fingerprinting Technology [J]. Scientia Agricultura Sinica, 2022, 55(21): 4252-4264.
[11] KANG Chen,ZHAO XueFang,LI YaDong,TIAN ZheJuan,WANG Peng,WU ZhiMing. Genome-Wide Identification and Analysis of CC-NBS-LRR Family in Response to Downy Mildew and Powdery Mildew in Cucumis sativus [J]. Scientia Agricultura Sinica, 2022, 55(19): 3751-3766.
[12] CHEN FengQiong, CHEN QiuSen, LIN JiaXin, WANG YaTing, LIU HanLin, LIANG BingRuoShi, DENG YiRu, REN ChunYuan, ZHANG YuXian, YANG FengJun, YU GaoBo, WEI JinPeng, WANG MengXue. Genome-Wide Identification of DIR Family Genes in Tomato and Response to Abiotic Stress [J]. Scientia Agricultura Sinica, 2022, 55(19): 3807-3821.
[13] YuXia WEN,Jian ZHANG,Qin WANG,Jing WANG,YueHong PEI,ShaoRui TIAN,GuangJin FAN,XiaoZhou MA,XianChao SUN. Cloning, Expression and Anti-TMV Function Analysis of Nicotiana benthamiana NbMBF1c [J]. Scientia Agricultura Sinica, 2022, 55(18): 3543-3555.
[14] BaoHua CHU,FuGuo CAO,NingNing BIAN,Qian QIAN,ZhongXing LI,XueWei LI,ZeYuan LIU,FengWang MA,QingMei GUAN. Resistant Evaluation of 84 Apple Cultivars to Alternaria alternata f. sp. mali and Genome-Wide Association Analysis [J]. Scientia Agricultura Sinica, 2022, 55(18): 3613-3628.
[15] XIE Bin,AN XiuHong,CHEN YanHui,CHENG CunGang,KANG GuoDong,ZHOU JiangTao,ZHAO DeYing,LI Zhuang,ZHANG YanZhen,YANG An. Response and Adaptability Evaluation of Different Apple Rootstocks to Continuous Phosphorus Deficiency [J]. Scientia Agricultura Sinica, 2022, 55(13): 2598-2612.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd