Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (1): 102-110.doi: 10.3864/j.issn.0578-1752.2012.01.012

• HORTICULTURE • Previous Articles     Next Articles

Cloning and Expression of the Sorbitol Dehydrogenase Gene Family in Apple

 LIANG  Dong, WU  Shan, WANG  Su-Fang, MA  Feng-Wang   

  1. 1.西北农林科技大学园艺学院/旱区作物逆境生物学国家重点实验室，陕西杨凌 712100
  • Received:2011-06-30 Online:2012-01-01 Published:2011-11-02

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Abstract: 【Objective】 The objective of this experiment was to further investigate the molecular characteristics of sorbitol dehydrogenase (SDH) in apple. 【Method】 The cDNAs and gDNAs of SDH gene family were isolated from the fruit of Malus domestica cv. Gala, and the mRNA expression levels of SDH gene family during leaf and fruit development were studied. 【Result】Twelve cDNAs of SDH were cloned from apple fruit, in turn being named MdSDH7-MdSDH18. Intron/exon structure analysis indicated that MdSDH18 contained 6 extons and 5 introns, whereas others contained 2 extons and 1 intron. The mRNA expression levels and enzyme activities of SDH were detected during leaf and fruit developments. According to the amino acid sequence homology, these SDHs were divided into two groups, A and B. During the fruit development, the mRNA expression levels of all SDHs at middle phase and late phase were higher than that of early phase, however, the changes of SDH enzyme activity were opposite to gene expression patterns. During leaf development, mRNA expression levels of A-SDH were in the order of young leaf ＞ senescence leaf ＞ mature leaf, with activities showing similar patterns to gene expression. However, mRNA expression   levels of B-SDH was in the order of senescence leaf ＞mature leaf ＞ young leaf. 【Conclusion】 These results further suggest that SDH is a gene family in apple, and the expression of gene family members shows different patterns during leaf and fruit developments.

Key words: apple, sorbitol dehydrogenase, gene family, gene expression

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