Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (21): 4481-4487.doi: 10.3864/j.issn.0578-1752.2011.21.017

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Influence of Infusing Amino Acids into the External Pudendal Artery on Nutrient Uptake in the Mammary Gland of Dairy Goats

 WANG  Qiang, AO  Chang-Jin, GA  尔Di, LU  De-Xun, GAO  Min, DUAN  Bin, FENG  Zi-Qi   

  1. 1.内蒙古农业大学动物科学学院,呼和浩特 010018
    2.内蒙古农牧业科学院动物营养研究所,呼和浩特 010030
    3.内蒙古自治区产品质量检验研究院,呼和浩特 010010
  • Received:2010-10-20 Online:2011-11-01 Published:2011-01-05

Abstract: 【Objective】 An experiment was conducted to study the effects of different balance models of amino acids in the artery blood flow by infusing amino acids into the external pudendal artery on nutrient uptake in the mammary gland of dairy goats. 【Method】 Three dairy goats at 2-3-year old of age with similar body weight and milk yield were intubated in the external pudendal artery and the abdominal subcutaneous vein, in this study. Different balance models of amino acids in the blood flow were made by infusing amino acids into the external pudendal artery. Arterio-venous plasma, at the start and the end of infusion, were collected to measure the changes of milk protein precursors (MPP), milk fat precursors (MFP) and milk lactose precursors (MLP) concentrations as well as nutrients uptake in the mammary gland. 【Result】 The uptake of MPP and MFP in the mammary gland was step up after infusing amino acids into the external pudendal artery (P<0.05). Compared with the control group, the uptake of MLP by the mammary gland was increased after infusing, but there was no significant difference in the ideal amino acids model group (P>0.05), meanwhile the difference was significant in the balance model group (P<0.05). The milk production and milk content of the dairy goats were significantly different in the two groups (P<0.05) compared with the control group. 【Conclusion】 Under the conditions of the experiment, the amino acids balance could change the uptake of milk components precursors in the mammary gland and in favor of the synthesis of milk. The uptake of milk components precursors in the mammary gland was optimum when the amino acid model was arrived at the ideal balance level (AABI=0.972).

Key words:

[1]Piepenbrink M S, Overton T R, Clark J H. Response of cows fed a low crude protein diet to ruminally protected methionine and lysine. Journal of Dairy Science, 1996, 79: 1638-1646.

[2]Mabjeesh S J, Kyle C E, MacRae J C, Bequette B J. Lysine metabolism by the mammary gland of lactating goats at two stages of lactation. Journal of Dairy Science, 2000, 83: 996-1003.

[3]Bequette B J, Backwell F R C, Crompton L A. Current concepts of amino acid and protein metabolism in the mammary gland of the lactating ruminant. Journal of Dairy Science, 1998, 81: 2540-2559.

[4]Guinard J, Rulquin H. Effects of graded amounts of duodenal infusions of lysine on the mammary uptake of major milk precursors in dairy cows. Journal of Dairy Science, 1994, 77: 3565-3576.

[5]Guinard J, Rulquin H. Effect of graded levels of duodenal infusions of casein on mammary uptake in lactating cows. 2. individual amino acids. Journal of Dairy Science, 1994, 77: 3304-3315.

[6]Guinard J, Rulquin H. Effects of graded amounts of duodenal infusions of methionine on the mammary uptake of major milk precursors in dairy cows. Journal of Dairy Science, 1995, 78: 2196-2207.

[7]Mabjeesh S J, Kyle C E, MacRae J C, Hanigan M D, Bequette B J. Vascular sources of amino acids for milk protein synthesis in goats at two stages of lactation. Journal of Dairy Science, 2002, 85: 919-929.

[8]Bach A, Huntington G B, Calsamiglia S, Stern M D. Nitrogen metabolism of early lactation cows fed diets with two differ levels of protein and different amino acid profile. Journal of Dairy Science, 2000, 83: 2585-2595.

[9]甄玉国. 内蒙古白绒山羊氨基酸利用和蛋白质周转规律的研究[D]. 呼和浩特: 内蒙古农业大学, 2002.

Zhen Y G. Study on utilization of amino acids and protein turnover in Inner Mongolian White Cashmere Goats[D]. Hohhot: Inner Mongolia Agriculture, 2002. (in Chinese)

[10]Boisen S, Hvelplund T, Weisbjerg M R. Ideal amino acid profiles as a basis for feed protein evaluation. Livestock Production Science, 2000, 64: 239-251.

[11]Meinnel T, Mechulam Y, Blanquet S. Methionine as translation start signals: a review of the enzymes of in Escherichia coli. Biochimistry, 1993, 75: 1061-1075.

[12]Metcalf J A, Crompton L A, Wray-cahen D, Lomax M A, Sutton J D, Beever D E, Macrae J C, Bequette B J, Backwell F R C, Lobley G E. Responses in milk constituents to intravascular administration of two mixtures of amino acids to dairy cows. Journal of Dairy Science, 1996, 79: 1425-1429.

[13]D’-Mello J P F. Amino Acids in Animal Nutrition. CABI Publishing, 2003.

[14]谢宝柱. 真胃灌注酪蛋白或尿素对奶山羊泌乳性能和乳腺氨基酸代谢的影响[D]. 泰安: 山东农业大学, 2006.

Xie B Z. Effects of abomosal infusion of casein or urea on lactating and metabolism of plasma amino acids mammary in dairy goats[D]. Taian: Shandong Agriculture University, 2006. (in Chinese)

[15]段  斌. 阴外动脉氨基酸平衡对奶山羊乳腺摄取乳成分前体物的影响[D]. 呼和浩特: 内蒙古农业大学, 2010.

Duan B. Effect of external pudic artery amino acid balance on milk composition precursors intaked by mammary gland in milk goat[D]. Hohhot: Inner Mongolia Agriculture University, 2010. (in Chinese)

[16]孙满吉. 阴外动脉灌注乙酸盐,氨基酸和葡萄糖对奶山羊乳腺营养物质摄取和乳成分影响的研究[D]. 内蒙古: 内蒙古农业大学, 2008.

Sun M J. Effects of infusing acetates, amino acids and glucose into external pudic artery on nutrient uptake of mammary gland and milk components in lactating does[D]. Inner Mongolia: Inner Mongolia Agricultural University, 2008. (in Chinese)

[17]程光民, 林雪彦, 李福昌, 陈凤梅, 王中华, 刘建胜, 伏桂华. 瘤胃灌注乙丙酸不同摩尔比混合挥发性脂肪酸对奶山羊乳脂合成的影响. 畜牧兽医学报, 2009, 40(7): 1028-1036.

Chen G M, Lin X Y, Li F C, Chen F M, Wang Z H, Liu J S, Fu G H. The influence of acetate and propionate molar ration in ruminally infused volatile fatty acid mixtures on milk fat synthesis in lactating goats. Acta Veterinaria et Zootechnica Sinica, 2009, 40(7): 1028-1036. (in Chinese)

[18]Rigout S, Lemosquet S, van Eys J E, Blum J W, Rulquin H. Duodenal glucose increases glucose fluxes and lactose synthesis in grass silage-fed dairy cows. Journal of Dairy Science, 2002, 85: 595-606.

[19]Vernon R G. Lipid metabolism in the adipose tissue of ruminant animals. Progress in Lipid Research, 1981, 19: 280-353.

[20]Griinari J M, McGuire M A, Dwyer D A, Bauman D E, Palmquist D L. Role of insulin in the regulation of milk fat synthesis in dairy cows. Journal of Dairy Science, 1997, 80: 1076-1084.

[21]程光民. 反刍家畜乳脂合成营养调控研究[D]. 泰安: 山东农业大学, 2005.

Chen G M. Study on nutritional regulation of milk fat synthesis in lactating ruminant[D]. Taian: Shandong Agriculture University, 2005. (in Chinese)

[22]Cant J P, Trout D R, Qiao F, McBride B W. Milk composition responses to unilateral arterial infusion of complete and histidine-lacking amino acid mixtures to the mammary glands of cows. Journal of Dairy Science, 2001, 84: 1192-1200.

[23]Bequette B J, Hanigan M D, Calder A G, Reynolds C K, Lobley G E, MacRae J C. Amino acid exchange by the mammary gland of lactating goats when histidine limits milk production. Journal of Dairy Science, 2000, 83: 765-775.

[24]Mackle T R, Dwyer D A, Ingvartsen K L, Chouinard P Y, Ross D A, Baumam D E. Evaluation of whole blood and plasma in the interorgan supply of free amino acids for the mammary gland of lactating dairy cows. Journal of Dairy Science, 2000, 83: 1300-1309.
[1] YU QiLong,HAN YingYan,HAO JingHong,QIN XiaoXiao,LIU ChaoJie,FAN ShuangXi. Effect of Exogenous Spermidine on Nitrogen Metabolism of Lettuce Under High-Temperature Stress [J]. Scientia Agricultura Sinica, 2022, 55(7): 1399-1410.
[2] YANG ShiMan, XU ChengZhi, XU BangFeng, WU YunPu, JIA YunHui, QIAO ChuanLing, CHEN HuaLan. Amino Acid of 225 in the HA Protein Affects the Pathogenicities of H1N1 Subtype Swine Influenza Viruses [J]. Scientia Agricultura Sinica, 2022, 55(4): 816-824.
[3] YAN TongJing,ZHANG DeQuan,LI Xin,LIU Huan,FANG Fei,LIU ShanShan,WANG Su,HOU ChengLi. Effects of Very Fast Chilling on Flavor Quality in Chilled Lamb [J]. Scientia Agricultura Sinica, 2022, 55(15): 3029-3041.
[4] ZHU Yin,ZHANG Yue,YAN Han,LÜ HaiPeng,LIN Zhi. Enantiomeric Analysis of Free Amino Acids in Different Teas [J]. Scientia Agricultura Sinica, 2021, 54(4): 804-819.
[5] HOU ChengLi,HUANG CaiYan,ZHENG XiaoChun,LIU WeiHua,YANG Qi,ZHANG DeQuan. Changes of Antioxidant Activity and Its Possible Mechanism in Tan Sheep Meat in Different Postmortem Time [J]. Scientia Agricultura Sinica, 2021, 54(23): 5110-5124.
[6] PAN ZhengYan,LIU Bo,JIANG HongBo,YAO JiPan,BAI YuanJun,XU ZhengJin. Effect of Panicle Neck Blast on Grain Yield and Stem Node Metabolites at the Rice Filling Stage [J]. Scientia Agricultura Sinica, 2020, 53(20): 4177-4188.
[7] ZHU ZiJian,CHEN SiYu,SU Jun,TAO YongSheng. Correlation Analysis Between Amino Acids and Fruity Esters During Spine Grape Fermentation [J]. Scientia Agricultura Sinica, 2020, 53(11): 2272-2284.
[8] ZHANG WenQiang,CHEN QingJun,ZHANG GuoQing,SHI ShiDa,CAO Na,ABLAT· Tohtirjap,GUO YuXin,LIN WenCai. Effects of Protein Supplements on Agronomic Characters and Quality of the Mushroom Agaricus bisporus [J]. Scientia Agricultura Sinica, 2020, 53(10): 2091-2100.
[9] Wei ZHANG,JinJun DAI,XueHai YANG,JinTao WEI,MingXin CHEN,JunPeng HU,ShaoWen HUANG. Evaluation of Apparent Metabolic Energy, Nitrogen Corrected Metabolic Energy, Biological Value of Protein and Ileal Digestibility of Amino Acid of Yeast Hydrolysate for Broilers [J]. Scientia Agricultura Sinica, 2019, 52(20): 3685-3694.
[10] HUA WeiYi,LIU YiMing,XU Fei,LU YongQiang,KONG Mei,WANG HaiTing,HUANG HuiLi,WANG HongLei,WU LianYong,LI XiuBo. The Safety Evaluation of Cefalonium Intramammary Infusion (Dry Cow) [J]. Scientia Agricultura Sinica, 2019, 52(2): 359-366.
[11] YAN ChaoQun,LI ShuaiPeng,ZHANG Shen,XIE Shun,WEI KaiYun,HUANG XianHui. Residue Depletion Study and Withdrawal Period for Cefalonium Intramammary Infusion (Dry cow) in Bovine Milk [J]. Scientia Agricultura Sinica, 2019, 52(2): 367-375.
[12] SUN JuanJuan, A LaMuSi, ZHAO JinMei, XUE YanLin, YU LinQing, YU Zhu, ZHANG YingJun. Analysis of Amino Acid Composition and Six Native Alfalfa Cultivars [J]. Scientia Agricultura Sinica, 2019, 52(13): 2359-2367.
[13] HAN YaFei, WANG XueDe, ZHENG YongZhan, MEI HongXian, WEI AnChi, LIU YanYang. Study on Changes of Sesame Protein Content and Its Components of Yuzhi 11 Sesame Seed During Growth Period [J]. Scientia Agricultura Sinica, 2018, 51(4): 652-661.
[14] YuLing YANG, Lei ZHOU, Yuan YOU, XiaoZhi TANG, SuMeng WEI. The Effects of Oxidation on Textural Properties and Water Holding Capacity of Heat-Induced Myofibrillar Protein Gel [J]. Scientia Agricultura Sinica, 2018, 51(18): 3570-3581.
[15] ZHANG Hui, WU ShengYong, WANG XiaoQing, LEI ZhongRen. Changes in the Contents of Proteins and Free Amino Acid in haemolymph of Delia antique Adult Infected by Beauveria bassiana
 
[J]. Scientia Agricultura Sinica, 2017, 50(3): 591-598.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!