Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (21): 4474-4480.doi: 10.3864/j.issn.0578-1752.2011.21.016

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Comparison of Difference and Ontogenetic Expression of EAATs mRNA in the Small Intestine of Broiler Chick Embryo

 LI  Xiang-Guang, YAN  Hui-Chao, ZENG  Pei-Ling, ZHANG  De-Xiang, WANG  Xiu-Qi   

  1. 1.华南农业大学动物科学学院
  • Received:2010-10-18 Online:2011-11-01 Published:2011-06-10

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Abstract: 【Objective】The objective of this study was to compare the difference and ontogenetic expression of glutamate transporters (EAAT2 and EAAT3) mRNA in small intestine of broiler chick embryo. Two breeds were selected, one was Wens Yellow-Feathered Chicken (WYFC, a Chinese local breed which grows slowly and marketed age for female is over 100 days) and the other was White Recessive Rocks Chicken (WRRC). 【Method】 Ninety-six fertilized eggs of each breed which had a similar weight were assigned into 6 groups for hatching. Sixteen small intestinal samples from each group were collected on embryonic days 9 (E9), 12 (E12), 14 (E14), 17 (E17), 19 (E19) and day of hatch (DOH) after the eggs were weighed. The expressions of EAATs mRNA were determined using real-time RT-PCR by relative quantification method. 【Result】The resuts showed that weight of embryonated egg on E9, E12, E14, E17 and E19, and hatchling of WRRC was significantly higher than that of WYFC (P＜0.01). Ontogenetic patterns of EAAT2 mRNA were similar in the 2 breeds，which downregulated from E9 to E14 and upregulated on E17, followed by an downregulation to DOH. In comparison between the two breeds, there was a highly significant difference (P＜0.01) on E12, E17, E19 and DOH and a significant difference (P＜0.05) on E14. Ontogenetic patterns of EAAT3 mRNA were upregulated along with embryonic development in both 2 breeds, which showed a highly significant difference (P＜0.01) on E19 and a significant difference (P＜0.05) on E12. The abundance of EAAT2 and EAAT3 mRNA in the small intestine of WYFC was higher than that of WRRC and affected by embryonic days. There was an interaction of breed×embryonic day on the both EAAT2 and EAAT3 mRNA abundance. 【Conclusion】 The expression of EAATs mRNA differed in breeds as well as embryonic days. Ontogenetic patterns of EAAT2 and EAAT3 mRNA were different.

Key words: EAAT2, EAAT3, chick embryo, small intestine, ontogeny

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