Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (21): 4333-4339.doi: 10.3864/j.issn.0578-1752.2011.21.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Identification and Gene Mapping of a Novel Short Root Hair Mutant in Rice

 DING  Wo-Na, TONG  Yan-Li, WU  Jing, ZHU  Shi-Hua   

  1. 1.宁波大学科学技术学院/植物分子生物学研究室，浙江宁波 315211
    2.浙江大学生命科学学院/植物生理学与生物化学国家重点实验室，杭州 310058
  • Received:2011-04-22 Online:2011-11-01 Published:2011-05-27

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Abstract: 【Objective】The identification and cloning of novel root hair-related genes in rice would play an important role in understanding the molecular genetic mechanisms of root hair development.【Method】A rice (Oryza sativa L.) mutant was isolated from a T-DNA insertion mutant library. Solution culture, phenotypic study, statistics analysis of segregation of different phenotypes as well as gene mapping by map-based cloning were used to study the phenotypic and genetic characteristics of the Ossrh1 mutant and map the OsSRH1 gene. 【Result】 The root hairs of young mutant seedlings were shorter and only 36% of the wild type. Genetic analysis indicated that the mutation was controlled by a single recessive nuclear gene. To map the OsSRH1 gene, an F2 population was generated by crossing the mutant Ossrh1 with wild type Kasalath. OsSRH1 was firstly mapped between the microsatellite markers RM3183 and RM193 on chromosome 6 with genetic distance of 0.9 cM and 1.0 cM, respectively. Three new polymorphic STS (sequence-tagged site) markers were developed in the region. OsSRH1 was finally mapped between markers T1757 and T1768 with a distance of 115 kb. 【Conclusion】 The Ossrh1 mutant is controlled by a recessive nuclear gene, which is located on chromosome 6, between T1757 and T1768 with a physical distance of 115 kb.

Key words: rice (Oryza sativa L.), short root hair mutant, genetic analysis, gene mapping

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