一个水稻短根毛突变体的鉴定和基因定位

doi:10.3864/j.issn.0578-1752.2011.21.001

Abstract

Abstract: 【Objective】The identification and cloning of novel root hair-related genes in rice would play an important role in understanding the molecular genetic mechanisms of root hair development.【Method】A rice (Oryza sativa L.) mutant was isolated from a T-DNA insertion mutant library. Solution culture, phenotypic study, statistics analysis of segregation of different phenotypes as well as gene mapping by map-based cloning were used to study the phenotypic and genetic characteristics of the Ossrh1 mutant and map the OsSRH1 gene. 【Result】 The root hairs of young mutant seedlings were shorter and only 36% of the wild type. Genetic analysis indicated that the mutation was controlled by a single recessive nuclear gene. To map the OsSRH1 gene, an F2 population was generated by crossing the mutant Ossrh1 with wild type Kasalath. OsSRH1 was firstly mapped between the microsatellite markers RM3183 and RM193 on chromosome 6 with genetic distance of 0.9 cM and 1.0 cM, respectively. Three new polymorphic STS (sequence-tagged site) markers were developed in the region. OsSRH1 was finally mapped between markers T1757 and T1768 with a distance of 115 kb. 【Conclusion】 The Ossrh1 mutant is controlled by a recessive nuclear gene, which is located on chromosome 6, between T1757 and T1768 with a physical distance of 115 kb.

Key words: rice (Oryza sativa L.), short root hair mutant, genetic analysis, gene mapping

DING Wo-Na, TONG Yan-Li, WU Jing, ZHU Shi-Hua. Identification and Gene Mapping of a Novel Short Root Hair Mutant in Rice[J].Scientia Agricultura Sinica, 2011, 44(21): 4333-4339.

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Identification and Gene Mapping of a Novel Short Root Hair Mutant in Rice

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