Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (20): 4317-4325.doi: 10.3864/j.issn.0578-1752.2011.20.021

• VETERINARY SCIENCE • Previous Articles     Next Articles

Study on Polymorphisms of IRAK2 Gene and Its Association with Mastitis in Chinese Holstein Cattle

 SUN  Shu-Xia, WANG  Chang-Fa, ZHANG  Lian-Jiang, JU  Zhi-Hua, HUANG  Jin-Ming, LI  Jian-Bin, ZHONG  Ji-Feng, ZHANG  Jia-Bao, LI  Qiu-Ling   

  1. 1.吉林大学实验动物中心
    2.山东省农业科学院奶牛研究中心
    3.吉林农业科技学院动物科学学院
  • Received:2010-09-11 Online:2011-10-15 Published:2010-11-24

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Abstract: 【Objective】 The associations among five single nucleotide polymorphisms (SNPs) in interleukin-1 receptor associated kinase 2 (IRAK-2) gene and somatic cell score (SCS) in Chinese Holstein Cattle were investigated. 【Method】 A herd of 1 292 dairy cows, 129 Luxi Yellow Cattle and 32 Bohai Black Cattle from Shandong province and Tianjin were used as experimental materials. Polymerase chain reaction (PCR) - restriction fragment length polymorphism (RFLP), created restriction site (CRS)-PCR and sequencing methods were applied to analyze the polymorphisms of five loci (g.28879, g.28916, g.29212, g.40035, and g.400120) in IRAK-2 gene. The associations between SNPs and SCS were analyzed by the least-squares method as applied in the GLM procedure of SAS 8.1 and haplotype analysis was performed by SHEsis software.【Result】The results of haplotype analysis of five SNPs showed that 30 different haplotypes and 71 haplotype combinations were identified. The value of SCS of individuals with H21H23 (TTAGGCTCCC) was the lowest through the result of association between polymorphisms of IRAK-2 gene and SCS. Statistical analyses revealed significant associations between genotype CC and CT in position 28 879, and between GG and AG in position 28 916. The SCS value of genotype CC and GG was lower than that of CT and AG, respectively (P＜0.05), whereas no significant differences were detected among the positions 29212, 40035, 40120 and SCS (P＞0.05).【Conclusion】The haplotype combination H21H23 was a favorable haplotype combination to the SCS. It may be used as a possible candidate for the molecular markers of mastitis resistance in dairy cattle breeding program.

Key words: ChineseHolsteinCattle, IRAK2, SNPs, SCS, haplotype

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