Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (9): 1926-1932 .doi: 10.3864/j.issn.0578-1752.2010.09.020

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

In Silico Study on a New Orthologs SLC25A3 and It’s Experimental Verification in Porcine Ovary

PAN Zeng-xiang, ZHANG Jin-bi, LIN Fei, LI Qi-fa, XIE Zhuang, LIU Hong-lin
   

  1. （南京农业大学动物科技学院）
  • Received:2009-09-11 Revised:2009-11-28 Online:2010-05-01 Published:2010-05-01

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Abstract:

【Objective】 The objective of this study is to efficiently obtain the structural and functional information of orthologs. 【Method】 The approach of gene-oriented ESTs annotation based on model organisms, in silico analysis and the method of primers extension were combined in the study.【Result】 An orthologs SLC25A3 (Solute Carrier Family 25, Member 3)by the approach of gene- oriented ESTs annotation based on human cDNA sequences was identified, and then the homologous ESTs expressed in porcine ovary were selected for in silico cloning and experimental verification, and ulmately the structural and functional information of the orthologs SLC25A3 were obtained by bioinformatic methods.【Conclusion】 Combining the gene-oriented ESTs annotation method and the relative bioinformatcic tools, the sequene and functional information of orthologs could be obtained fastly and directly, and the transformation or communication of the valuable information between different species could be realized, thus providing reliable chioces for sequence analysis and functional annotation in comparative genomic research.

Key words: pig, orthologs, comparative genomics, in silico study, bioinformaitics

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