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Table of Content

    01 May 2010, Volume 43 Issue 9
    CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS
    Strategies on the Safety of Selectable Marker Genes in Transgenic Plant
    LI Wen-feng,JI Jing,WANG Gang,WANG Hai-yong,NIU Bao-long
    Scientia Agricultura Sinica. 2010, 43(9):  1761-1770 .  doi:10.3864/j.issn.0578-1752.2010.09.001
    Abstract ( 979 )   PDF (405KB) ( 1445 )   Save
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    Selectable marker genes encoding antibiotic and herbicide are widely used in transgenic plants and have a potential risk to the environment and human health. Currently, there are three kinds of strategies to improve security in using marker genes in transgenic plants: One approach is to develop the use of uncontested biosafety marker genes, the second is to use transgenic plants-removed selectable marker genes and the third is the use of non-selective marker gene transformation system. In this review, each of the strategy principles and problems are highlighted. Moreover, the perspectives are discussed to improve the safety of selectable marker genes in transgenic plants.

    Analysis on the Internal Transcribed Spacers (ITS) Sequences and Phylogenetics of Mulberry (Morus)
    CHEN Ren-fang,YU Mao-de,LIU Xiu-qun,CHEN Long-qing
    Scientia Agricultura Sinica. 2010, 43(9):  1771-1781 .  doi:10.3864/j.issn.0578-1752.2010.09.002
    Abstract ( 1162 )   PDF (479KB) ( 1193 )   Save
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    【Objective】 The phylogeny of mulberry (Morus) was investigated by using ITS sequences and it will provide useful information for mulberry breeding, germplasm DNA fingerprint identification, classification and evolution. 【Method】 Seventy-three mulberry (Morus) resources were collected from different localities in China and sequenced ITS for all samples by sanger dideoxy method. Furthermore, other ITS sequences of Moraceae and Morus were also downloaded from GenBank and aligned with the sequences obtained in this study by Clustalx1.83c software. Then, the G+C content, divergence and similarity among sequences were analyzed by DNAstar software. Finally, based on the ITS sequences, phylogenetic trees were reconstructed by PAUP Version4.0b10 and Mrbayes softwares. 【Result】 Seventy-three complete internal transcribed spacer (ITS) sequences of Morus were obtained. The length of Morus ITS sequences is 576 bp, and its G + C content is 59.55% (thereinto, ITS1:174 bp, G+C:61.49%, ITS2:302 bp, G+C:62.25%, 5.8S:100 bp, G+C:48.00%). It has 166 variable sites in total (thereinto, ITS1: 100, ITS2: 66, 5.8S: 0), some variable sites are species-specific and can be used as DNA molecular markers of mulberry (Morus) resources. The largest divergence is 4.0. Compared with the other cultivated species, the variation of M. bombycis is bigger, which has differences ranging from 0.9 to 1.4 to the others. Based on the ITS system position of Morus, M. bombycis is between Milicia excelsa (MEU93585) and Streblus glaber (DQ499105) and mulberry (Morus) and Milicia excelsa (MEU93585) have a closest relationship. In addition, Xinjiang M. nigra and North America M. murrayana (FJ605515) are the most primordial species. The evolutionary order of Morus is as M. nigra and M. murrayana→M. alba→M. cathayana →M. wittiorum. 【Conclusion】 ITS is suitable for phylogenetic analysis of Morus. The results of the length of ITS, the content of G+C%, and the variation sites have provide a molecular biological basis for the classification, phylogeny, DNA fingerprinting and cross breeding parentage choice of mulberry. Morus derived from Laurasia move from north to south. The phylogeny of Morus can be reflected indirectly by geographical distribution.

    TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY
    Quantitative Research of Dynamic Models of the Main Geometric Parameters of Rice Root System of Different Varieties Under Different Nitrogen Conditions
    LIU Yong-xia,YUE Yan-bin,LIU Yan,CAO Hong-xin,GE Dao-kuo,WEI Xiu-fang
    Scientia Agricultura Sinica. 2010, 43(9):  1782-1790 .  doi:10.3864/j.issn.0578-1752.2010.09.003
    Abstract ( 971 )   PDF (333KB) ( 761 )   Save
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    【Objective】 The aims of this study were to build the biomass-based main geometric parameter dynamic models of rice root system according to the relationships between the total root length, the total root volume, the total root surface area per plant and the dry weight per plant of different rice cultivars under different fertilizer levels. 【Method】 Three rice cultivars, ‘Liangyou108’ (Hybrid long-shaped rice, V1), ‘86you8’ (Hybrid round-shaped rice, V2), and ‘Nanjing43’ (Round-shaped rice,V3), were planted in pots with two fertilizer levels: fertilizer (N:3.6 g/pot, P2O5:0.8 g/pot, K2O:3.6 g/pot) , and no fertilizer (with loamy soil) to obtain the root system morphological characteristics, the dry weight, etc., which were processed by SPSS v13.0 and Excel 2007. The models developed in this paper were verified using RMSE.【Result】The biomass-based dynamic models of the total root length were set up through analyzing relationships between the total root length and the biomass per plant from the experimental data in 2008, and then, the biomass-based dynamic models of the total root volume and the total root surface area per plant also were developed according to the principles of solid geometry, which were tested by using the experiment data in 2009. The results showed that the RMSE between the simulated values and the observed values of the total root length, the total root volume, and the total root surface area per plant were 490.660 cm/plant, 131.859 cm2/plant, and 2. 532 cm3/plant, respectively. 【Conclusion】 The models developed in this paper were reliable with mechanics, general adaptability, and interpretative characteristics in a certain extent, which would lay a basis for developing Functional-Structure Rice Models.

    Absorption, Accumulation and Distribution of Zinc in High-Yielding Winter Wheat
    DANG Hong-kai,LI Rui-qi,SUN Ya-hui,ZHANG Xin-wen,LI Yan-ming
    Scientia Agricultura Sinica. 2010, 43(9):  1791-1799 .  doi:10.3864/j.issn.0578-1752.2010.09.004
    Abstract ( 946 )   PDF (299KB) ( 823 )   Save
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    【Objective】 The objective of this experiment is to clarify the characteristics of zinc (Zn) absorption, accumulation and distribution in high-yielding winter wheat (with a grain yield 9 000 kg•hm-2). 【Method】 Field experiments were conducted in Gaocheng County, Hebei Province. Four winter wheat cultivars, Shimai 14, Jifeng 703, Shimai 12 and Shixin 828 during 2004-2005, and four cultivars, Temai 1, Shimai 12, Shixin 531 and Shixin 828 during 2005-2006, were used as experimental materials. Plant samples were collected from the plots at each growing stage for Zn concentration determination. 【Result】 The main results showed that the concentration of Zn in various above-ground organs of wheat was 9.5-112.5 mg•kg-1 at different growing stages. The organ with the highest Zn concentration changed with the growth center at different growing stages. The accumulation amount of Zn in leaf blades was the highest among all organs during early growing period, and the distribution percentage of Zn in leaf blades was more than 50% before jointing stage, higher than those in other organs. In late growing period, however, the accumulation amount of Zn in grains was the highest, and the distribution percentage of Zn in grains was 58.1% at maturity. The total accumulation amount of Zn in wheat plants during its life span ranged from 384.9 to 475.9 g•hm-2. The amount of Zn required for the formation of 100 kg grain yield ranged from 4.3 to 5.2 g. The net absorption, transportation of Zn and the contribution of all organs to grain Zn were all in the order of leaf blades > spikes > leaf sheaths > stems. 58.2%-60.3% of Zn in grains was redistributed from other organs, especially from leaves.【Conclusion】 The concentration and accumulation of Zn in all organs of wheat was high during early and middle growing periods, and the accumulation of Zn in grains during late growing period mainly depended on the redistribution from various organs. According to the characteristics of Zn absorption and accumulation, Zn should be applied as seed dressing or basal dressing, so as to accelerate the early growth and zinc absorption of wheat.

    Correlation Analysis Between Agronomic Traits and Yield of Rapeseed (Brassica napus L.) for High-Density Planting
    SONG Xi,LIU Feng-lan,ZHENG Pu-ying,ZHANG Xue-kun,LU Guang-yuan,FU Gui-ping,CHENG Yong
    Scientia Agricultura Sinica. 2010, 43(9):  1800-1806 .  doi:10.3864/j.issn.0578-1752.2010.09.005
    Abstract ( 1057 )   PDF (286KB) ( 1469 )   Save
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    【Objective】 The difference in the relation of agronomic traits and yield between rapeseeds suitable for mechanized high-density planting and conventional-density planting was studied. 【Method】 Twenty-two hybids (or lines) for high-density planting were studied in national winter rapeseed regional test from 2008 to 2009. The correlation and path analysis of nine major agronomic traits to yield per plot were conducted by contrast of seventy-two rapeseeds which are suitable for conventional-density planting.【Result】For the former, seeds per silique,the number of effective siliques on main inflorescence,the number of effective siliques per plant and density of siliques with yield per plot showed a significant or extremely significant correlation.The order of direct effect of traits on yield per plot was density of siliques (0.881)>branch height (-0.614)>plant height (0.586)>seeds per silique (0.360)>the number of branches (-0.144)>the number of effective siliques on main inflorescence (-0.143)>the number of effective siliques per plant (0.111)>effective length of main inflorescence (0.095)>1000-seed weight (-0.055). However, the number of branches,the number of effective siliques on main inflorescence,the number of effective siliques per plant,seeds per silique and branch height showed a significant or extremely significant correlation with yield per plot for rapeseeds suitable for conventional-density planting.The order of direct effect was the number of effective siliques per plant (0.412)>plant height (0.297)>the number of effective siliques on main inflorescence (0.260)>seeds per silique (0.249)>effective length of main inflorescence (-0.224)>1000-seed weight (0.156)>branch height (-0.104)>the number of branches (0.095)>the density of siliques (-0.003).【Conclusion】 Compared with rapeseeds suitable for conventional-density planting,the breeding aims of rapeseed selected specically for high-density planting should lay more emphasis on density of siliques and the number of effective siliques on main inflorescence. Furthermore, efforts in increase of the number of effective siliques per plant as well as seeds per silique and decrease of the number of null branches should be made.
    PLANT PROTECTION
    Activities and Active Components of Radix peucedani Capable of Inducing Rice Resistance to Blast Disease
    CHEN Gui-hua,TANG Wen-bang,ZHANG Zhi-fei,CAI Hai-lin,BAI Lian-yang
    Scientia Agricultura Sinica. 2010, 43(9):  1807-1814 .  doi:10.3864/j.issn.0578-1752.2010.09.006
    Abstract ( 972 )   PDF (328KB) ( 697 )   Save
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    【Objective】The active components of Radix peucedani capable of inducing rice resistance to blast disease were separated, purified and identified.【Method】Using activities of inducing resistance to blast disease as an activity monitoring parameter, acetone extract was isolated by silica-gel column to obtain the strongest part of the activity, and the main resistance inducing components from the strongest fractions were separated by using Sephadex LH-20 resin and the structures were analyzed by ultraviolet-vis, infra-red, ESI-MS, 1H-NMR and 13C-NMR spectrums.【Result】Seven fractions from A1 to A7 collected from silica-gel column were tested, A4, A5, A6 had the strongest resistance inducing activities.When the concentrations were 50 mg•L-1, the resistance inducing activities were 55.18%, 59.54%, and 54.36%, respectively. Then three active compounds, Q-1, Q-2, and Q-3, were separated from A4, A5, and A6 through Sephadex LH-20 resin and HPLC. When the concentrations were 10 mg•L-1, the resistance inducing activities of Q-1, Q-2, and Q-3 were 52.77%, 60.76%, and 60.28%, respectively. The spectroscopy analysis indicated that the 3 active components of inducing rice resistance to blast disease of Radix peucedani were peucedanocoumarin Ⅲ, peucedanocoumarin Ⅱ, pteryxin. In addition, peucedanocoumarin Ⅲ was isolated from Radix Peucedani for the first time in China. 【Conclusion】 It is concluded that the coumarin compounds are the most important substance of Radix peucedani capable of inducing rice resistance to blast disease.

    Analysis on the Population Status of the Overwintering Generation of the Clover Cutworm Scotogramma trifolii (Lepidoptera: Noctuidae) in Beijing
    ZHANG Yun-hui,CHENG Deng-fa,JIANG Yu-ying,ZHANG Yue-jin,SUN Jing-rui
    Scientia Agricultura Sinica. 2010, 43(9):  1815-1822 .  doi:10.3864/j.issn.0578-1752.2010.09.007
    Abstract ( 933 )   PDF (550KB) ( 1034 )   Save
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    【Objective】 Research on the variation of light catch and population status of the clover cutworm Scotogramma trifolii (Lepidoptera: Noctuidae) was carried out in order to provide a basis for effective prevention and control of it in North China. 【Method】 Vertical-looking radar and related equipment were used to carry out real-time monitoring of the migratory process of the clover cutworm, and the prevalent airflow was analyzed by the software of GRADS during the migrating peak and the Hysplit_4 software was employed for analyzing their migratory trajectory. Population status of the overwintering generation moth was also discussed. 【Result】Ovarian development concentrations at 2-3 grade and the sex ratio>1 during moth occurrence peak period, but the sex ratio<1 after the peak period. Simultaneous radar observations indicated that the nocturnally migrating clover cutworm mothes generally flew at altitudes of up to 600 m above ground level, with high density concentrations at about 300-500 m and these concentrations were coincident with favorable wind direction and wind speed. They can take 8 h sustainable flight in one night. Trajectory analysis of 12 h also showed that most of them came from Eerduosi, Huhehaote, Wumeng and other regions. in Inner Mongolia and some from north of Shaanxi and Shanxi provinces, migrated and diffused into northeast and southeast with the westerly airflow. 【Conclusion】 The overwintering generation of clover cutworm moth has a typical migratory habit, and it is of a great significance for eatly monitoring and forecasting them according to their migratory behavior.

    Progress in Research on Intelligentization of Field Weed Recognition and Weed Control Technology
    FAN De-yao,YAO Qing,YANG Bao-jun,ZHOU Ying-feng,GUAN Ze-xin,TANG Jian
    Scientia Agricultura Sinica. 2010, 43(9):  1823-1833 .  doi:10.3864/j.issn.0578-1752.2010.09.008
    Abstract ( 1024 )   PDF (287KB) ( 1687 )   Save
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    With the development of computer and electronic technology, field weed recognition and weed control technology move toward intelligentization and automatic weed control system has become a hot research subject. Automatic weed control system consists of four main modules,including guidance system module, image acquisition and processing module, weed recognition module and weed control module. The four modules were reviewed, the current problems were analyzed and future research were pointed out.

    SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT
    Study on the Effects of Different Land Use Patterns on Microbial Community Structure in Aquic Brown Soil by Utilizing PLFA Method
    BI Ming-li,YU Wan-tai,JIANG Zi-shao,MA Qiang,ZHANG Lu,XU Yong-gang
    Scientia Agricultura Sinica. 2010, 43(9):  1834-1842 .  doi:10.3864/j.issn.0578-1752.2010.09.009
    Abstract ( 1291 )   PDF (368KB) ( 1110 )   Save
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    【Objective】 Soil microbe is an important part of terrestrial ecosystem. The objective of this paper is to elucidate the effects of different land use patterns on soil microbial biomass and community structure. 【Method】 Soil microbial biomass and community structure were analyzed by phosphdipid fatty acid (PLFA) method, based on a consecutive 19 years field trial on the aquic brown soil in Shenyang suburbs of Northeast China. There were four land use patterns included, i.e. bare land, mown land, fallow land and agricultural land. Agricultural land was comprised of CK, M, NPK and NPK+M treatments. 【Result】 The changes of bacterial and fungal PLFA were similar to total PLFAs: that of fallow and mown land were the highest and followed by arable soils, and that was the lowest under bare land. The differences of PLFAs content among the treatments in agro-ecosystems were not significant. The highest PLFA was obtained under M treatment, followed by CK and NPK+M, and that was the lowest under NPK treatment. Increasing soil microbial PLFA was contributed to land abandonment and manure application rather than mineral fertilizers application. The value of G+ PLFA/G- PLFA in natural ecosystems was higher than that in agro-ecosystems; decline of the value was due to tillage and mineral fertilizers applied. The value of bacterial PLFA/fungi PLFA of fallow and mown land were lower than that of arable soils or bare land. Correlation between soil PLFAs content and soil nutrients showed that soil microbial biomass had significant correlation with soil organic matter and total nitrogen. 【Conclusion】 Microbial biomass and community structure were influenced significantly by long-term land use patterns.

    Phosphorus Status and Risk of Phosphate Leaching Loss from Vegetable Soil of Different Planting Years in Suburbs of Changsha
    QIN Hong-ling,QUAN Zhi,LIU Xin-liang,LI Ming-de,ZONG Yong,WU Jin-shui,WEI Wen-xue
    Scientia Agricultura Sinica. 2010, 43(9):  1843-1851 .  doi:10.3864/j.issn.0578-1752.2010.09.010
    Abstract ( 1158 )   PDF (301KB) ( 854 )   Save
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    【Objective】 The aim of the study was to develop an index to assess the environmental risk of P loss potential in vegetable soils with chronic difference of plantation in the suburbs of Changsha. 【Method】 Chemical methodology was used to study soil phosphorus status and the relationships between available P in soil and potential soil leaching P. 【Result】 The results showed that there was a significant linear relationship between Olsen-P and CaCl2-P or P concentration in soil solution. Olsen-P increased sharply when either CaCl2-P or P concentration in soil solution reached a certain level. It was confirmed that 80 mg•kg-1 of Olsen-P was the critical value of soil P leaching in the vegetable soil. P leaching probability over the critical was assessed by GIS and indicator kriging and four secondary risks of phosphorus leaching loss were defined. In the area with vegetable cropping for over 30 years (Chenjiadu) and 10-15 planting years (Huangxingzhen), the indices of phosphorus leaching loss risk were 3 and 2.93, respectively. These two areas belonged to strong secondary of risk of phosphate leaching loss. In the new vegetable planting field less than 2 years (Ningxiang), the index was 0.06, which was almost no risk of phosphorus leaching. 【Conclusion】 In vegetable soil in the suburban region of Changsha, the phosphorus leaching peotential is high and the phosphorus leaching loss is related to chronic length of vegetable cropping.

    Collectable Amounts and Suitability Evaluation of Straw Resource in China
    WANG Ya-jing,BI Yu-yun,GAO Chun-yu
    Scientia Agricultura Sinica. 2010, 43(9):  1852-1859 .  doi:10.3864/j.issn.0578-1752.2010.09.011
    Abstract ( 1039 )   PDF (298KB) ( 1905 )   Save
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    【Objective】 The total production of straw in China ranks the first place in the world. It is of great significance to evaluate the collectable straw amount and suitability of comprehensive utilization ways correctly for fully recognizing the developing potential of straw resources and optimizing utilization structure on the basis of modern agricultural production. 【Method】 Combining with relevant survey information about straw residual amount in the field, the collectable coefficients of straw resources were estimated according to the investigation results of crop stubble height in the Huanghe-Huihe-Haihe regions. And then the collectable amount of straw resources in China in 2005 was calculated based on the data of the total straw yield and the collectable coefficients of straw resources. At last, the suitability of straw used as fuel, feed, fertilizer, industrial raw material and edible fungi and respective collectable straw amount were quantitatively and qualitatively evaluated. 【Result】 The calculated result showed that the collectable amount of straw resources in China in 2005 was 68 595×104 t with the average collectable coefficient of 0.81. The collectable amount of food crops straw, economic crops straw and other crops straw was 49 231×104 t, 16 261×104 t and 3 103×104 t accounting for 71.77%, 23.71% and 4.52% of the total collectable amount of straw resources in China in 2005, respectively. The straw amount suitable for processing to feed was 58 764×104 t, accounted for 85.67% of the total collectable amount of straw resources in China in 2005. The straw amount of crop-residue incorporation and suitable for returning to field directly, suitable for cultivating edible fungi and industrial processing was 61 600×104 t, 58 700×104 t and 58 700×104 t, accounted for 75.00%, 85.00% and 85.00% of the total collectable amount of straw resources in China in 2005, respectively. 【Conclusion】 It is concluded from analysis that the collectable amount of straw resources in China is so abundant that can be used in many ways.

    The Possible Effect of Global Climate Changes on Cropping Systems Boundary in China Ⅱ. The Characteristics of Climatic Variables and the Possible Effect on Northern Limits of Cropping Systems in South China
    ZHAO Jin,YANG Xiao-guang,LIU Zhi-juan,CHENG Di-fang,WANG Wen-feng,CHEN Fu
    Scientia Agricultura Sinica. 2010, 43(9):  1860-1867 .  doi:10.3864/j.issn.0578-1752.2010.09.012
    Abstract ( 918 )   PDF (629KB) ( 1591 )   Save
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    【Objective】 Under the background of climate change, the changes of cropping boundary and its effect on yield in South China were studied. 【Method】 Based on the historical weather data of 281 stations in 15 provinces (cities, autonomous regions) in south China, the trends of ≥0℃ accumulated temperature and annual precipitations were analyzed briefly. The changes of grade 0 areas under climate change were studied according to the thermal indicators of heat. Furthermore, the changes of grade 1 areas were analyzed combining with the precipitation indicators. The changes of yield were studied based on the data of yield during 2000-2007. 【Result】 The trends of ≥0℃ accumulated temperature increased and the mean annual value was 60.05 ℃•d/10a; The trends of ≥10℃ accumulated temperature increased. The mean annual trends value of annual precipitations was 1.93 mm/10a, and the spatial differences were large. Compared with the situation before 1980, there was no significant change in the boundary of one-crop per year in South China; however, the change of boundary between two-crops and three-crops per year was marked, which moved with about 2′ to west and 20′ to north. The area of three-crops per year was enlarging, while the areas of two-crops and one-crop per year were reducing. The change of grade 1 area from 1980 was also significant. The south boundary area of two-crops in irrigated land and two/one-crop in dry land in Huanghuaihai area (part of South China) moved to north. Area of wheat-rice in Jianghuai plain or hill moved to north and enlarged. The north boundary of area of two/three-crops in paddy/dry land in Sichuan basin moved to south in the northeast part of Sichuan Province. The west and southeast boundary of area of two-crops in paddy field and two/one-crop in dry land in Southwest plateau or mountain moved to west, while there was no significant change on the area. The north boundary of area of two/three-crops in paddy field in middle-lower Yangtze plain or hill moved to north as well as the west boundary moved to west, and the area enlarged. The north boundary of area of later two/three-crops and thermal three-crops in South China moved to north and the area enlarged. The changes of cropping systems were benefit for increasing the yield in the most areas. 【Conclusion】The effect of climate change on the cropping system was so great that the boundary of multiple cropping moved to north and west, and the area enlarged. That was benefit for increasing the yield generally.
    HORTICULTURE
    Effect of bzr1 Gene Transformation on Characters of Fruit of Tomato
    LI Zhen,WEI Jia,JIA Cheng-guo,WANG Qiao-mei
    Scientia Agricultura Sinica. 2010, 43(9):  1868-1876 .  doi:10.3864/j.issn.0578-1752.2010.09.013
    Abstract ( 869 )   PDF (634KB) ( 1250 )   Save
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    【Objective】 The transformation of BZR1, a BR signal transduction component to tomato and its effect on characters of fruit of tomato was investigated in present study. 【Method】 The bzr1 gene was introduced into tomato cultivar Zhongshusihao via tumefaciens-mediated transformation and confirmed by molecular test and genetic analysis. The content of main quality components and pigments, as well as ethylene evolution rate was analyzed in fruits of transgenic tomato line and non-transgenic line (control) at four typical developmental stages. The expression of BR biosynthetic and regulated genes, as well as ethylene biosynthetic, response, and receptor genes was also analyzed. 【Result】 Significantly higher contents of soluble solids, organic acid, soluble proteins, vitamin C, lycopene and total carotenold, as well as the ethylene evolution rate in fruit of turning stage were observed in transgenic tomato line compared with the control. The expression of BR regulated gene LeBR1 was higher in transgenic line than in the control at the stage of seedling and early three developmental stages of fruit. The expression of LeACS4 gene encoding ACC synthase, which catalyzes the rate-limiting step of ethylene biosynthesis, was higher in the fruit of transgenic line than that of control from the stage of mature green to turning. The expression of ethylene response gene ERF1 and receptor gene LeETR1 and LeETR3 in fruit of breaker stage was significantly higher in transgenic line than in the control. 【Conclusion】 It was concluded from the study that the transformation of bzr1gene to tomato increased the content of various nutrients in fruit of turning stage, which is the edible part of tomato, and therefore improved the nutrient quality of tomato.

    Cloning of LeGRP2 Promoter from Tomato that Shows Root-Specific Expression in Arabidopsis
    LI Zhi-miao,YANG Yue-jian,YANG Fei,YE Qing-jing,WANG Rong-qing,RUAN Mei-ying,ZHOU Guo-zhi,YAO Zhu-ping,Yong-Ling Ruan
    Scientia Agricultura Sinica. 2010, 43(9):  1877-1882 .  doi:10.3864/j.issn.0578-1752.2010.09.014
    Abstract ( 940 )   PDF (387KB) ( 895 )   Save
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    【Objective】 The objective of the study was to clone a root-specific promoter from tomato for future use in generating new tomato materials through genetic engineering. 【Method】 Genomic walking technique was used to amplify the upstream regulatory sequence of LeGRP2 (glycine-rich protein), a tomato root-specific expression gene. To investigate the tissue expression pattern of the cloned regulatory sequence, an expression vector containing this sequence fused with GUS was constructed for transformation into Arabidopsis by using agrobacterium-mediated method. 【Result】 Using tomato genomic DNA as a template, a regulatory sequence (GenBank accession number: EU262719) 1 959 bp upstream of the LeGRP2 ATG site was amplified by two consecutive genomic walking steps. Sequence analyses revealed that it contained 9 copies of ROOTMOTIFTAPOX1, a known cis-acting element related to root-specific expression. Histochemical staining of transgenic Arabidopsis showed that GUS reporter gene was predominantly expressed in root in both 7 d seedlings and 40 d adult plants. 【Conclusion】 The LeGRP2 promoter was cloned, which displayed a strong root-specific expression pattern in Arabidopsis transformed with LeGRP2 promoter fused with gene GUS.

    Analysis of Expressed Sequence Tags in the Incompatible Interaction Between Watermelon and Fusarium oxysporum

    LÜ,Gui-yun,GUO Shao-gui,ZHANG Hai-ying,GENG Li-hua,XU Yong

    Scientia Agricultura Sinica. 2010, 43(9):  1883-1894 .  doi:10.3864/j.issn.0578-1752.2010.09.015
    Abstract ( 840 )   PDF (425KB) ( 895 )   Save
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    【Objective】The objective of the experiment is to study the mechanisms of watermelon resistance to Fusarium oxysporum by investigating the expression profiles of the related genes in the incompatible interaction. 【Method】 A cDNA library of the watermelon root tissues induced by Fusarium oxysporum f. sp. niveurn was constructed using the SMART protocol. The positive clones of the cDNA library were sequenced randomly and 3 895 desired ESTs were obtained for bioinformatics analysis. 【Result】 Total 1 756 unique sequences were annotated for the functions. Transcription factors, protein kinase, defence gene, shikimate-phenylpropanoid-lignin metabolism and JA biosynthesis were found in the incompatible interaction between watermelon and Fusarium oxysporum. 【Conclusion】 There were 36.3% of the annotated unique sequences had similar function with disease resistance and defense. This information showed us an elementary profile of the interaction mechanism between watermelon and Fusarium oxysporum. These results developed a good foundation for the further exploration of the interaction mechanism at the molecular level and the related important genes cloning.

    Effects of Grafting on Main Esters, Free Amino Acids Content and Related Enzyme Activities in Oriental Sweet Melon Peel and Flesh Tissues
    QI Hong-yan,GUAN Xiao-chuan,LI Yan,LI Jin-ran,QIU Li-yan
    Scientia Agricultura Sinica. 2010, 43(9):  1895-1903 .  doi:10.3864/j.issn.0578-1752.2010.09.016
    Abstract ( 885 )   PDF (379KB) ( 869 )   Save
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    【Objective】 The objective of this experiment is to define the primary mechanism of grafting on aroma volatiles in oriental sweet melon and elucidate the relationship between amino acids and volatile esters. The effects of grafting on main volatile esters, amino acids and related enzymes activities at different development periods in oriental sweet melon peel and flesh tissues were studied. 【Method】 Four main esters(i.e. ethyl acetate, 1-butanol, 2-methyl acetate, hexyl acetate, and phenylmethyl acetate) were collected using the solid-phase microextraction (SPME) and then quantified by GC. Also, free amino acids composition and content were investigated, and the key enzymes activities, which involved in esters biosynthesis were measured as well. 【Result】 Grafting increased ethyl acetate content in peel and flesh tissues and decreased hexyl acetate and phenylmethyl acetate content. 2-methyl-1-butyl acetate content in peel tissue increased, while that declined in flesh tissue in grafted melons. Sixteen kinds of free amino acids were detected in peel and flesh tissues of melon. Amino acids content gradually increased with fruits development, and the content in grafted melons was higher than own-rooted fruits at early stages; however, which lower than own-rooted fruits at middle and later stages. The biosynthesis relationship between valine, leucine and isoleucine and 2-methyl-1-butyl acetate were validated. The enzymes activities in peel (P<0.01) and flesh tissue (P<0.05) of ADH and AAT significantly decreased in grafted melons. 【Conclusion】 Grafting altered the main esters contents in oriental sweet melon peel and flesh tissues. In addition, the esters contents in peel tissue were higher than in flesh at maturity. It decreased most of free amino acids content, and parts of amino acids were involved in the biosynthesis of branched-chain esters. The enzymes activities of ADH and AAT were lower in grafted melons than own-rooted ones.

    STORAGE·FRESH-KEEPING·PROCESSING
    Analysis and Design of Premium Rates Determined for Weather-Based Index Insurance Contract of Citrus
    LOU Wei-ping,WU Li-hong,CHEN Hua-jiang,MAO Yu-ding
    Scientia Agricultura Sinica. 2010, 43(9):  1904-1911 .  doi:10.3864/j.issn.0578-1752.2010.09.017
    Abstract ( 953 )   PDF (362KB) ( 1519 )   Save
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    【Objective】 The objective of the research was to design weather-based index insurance contract for citrus and to provide technology for policy-guided agricultural insurance. 【Method】 Based on relationship between citrus yield loss rates and weather factors, the tail distribution of weather disaster risk led to catastrophic results was calculated by extreme value theory and premium rate was counted. Weather-based index insurance contact was designed based on the advantage of area yield crop insurance and weather index insurance. 【Result】 The pure premium rates and indemnity caused by meteorological disasters were calculated for each county in western Zhejiang. The pure premium rates for meteorological disasters insurance were from 1.8 percent to 3.0 percent in Chun’an, Lanxi, Jinhua, Lishui, and from 5.5 percent to 6.6 percent in Jiande, Changshan, Jiangshan, Longyou, and Quzhou. 【Conclusion】 Weather-based index insurance contact was designed for needs of policy-guided agricultural insurance. It will reduce the shortcomings existing in traditional agricultural insurance and abroad index insurance. Weather derivatives could be designed based on it to enter the financial markets.

    Analysis of the Structure of Extracted Cooked Cured-Meat Pigment by Different Spectra
    SUN Wei-qing,ZHOU Guang-hong,XU Xing-lian,PENG Zeng-qi
    Scientia Agricultura Sinica. 2010, 43(9):  1912-1918 .  doi:10.3864/j.issn.0578-1752.2010.09.018
    Abstract ( 934 )   PDF (377KB) ( 846 )   Save
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    【Objective】 The purpose of the experiment is to study the structure of cooked cured-meat pigment (CCMP). 【Method】 Four kinds of different spectra, which were electron paramagnetic resonance spectroscopy (EPR), HPLC-electrospray ionization high resolution mass spectrometry (HPLC-ESI-HR-MS), infrared spectrum (FTIR) and Raman spectrum, were used for analysis. 【Result】 EPR spectra of CCMP in an acetone glass presented a well-resolved triplet in the g3 region due to hyperfine splitting of the 14N nuclei of NO with an unpaired electron. The infrared and Raman spectrum of this complex exhibited a single nitrosyl stretch at the 1 653.31and 1 656.69 cm-1 corresponding to a NO- ligand state. The results of ESI-HR-MS analysis showed that the CCMP had a molecular weight consistent with monnitrosyi ferrohemochrome but had no molecular weight of dinitrosyi ferrohemochrome. 【Conclusion】 It is suggested that the structure of CCMP is a pentacoordinate mononitrosylheme complex.

    Effects of Water Soluble Extracts from Longan on Immune Regulation in Normal Mice
    SU Dong-xiao,ZHANG Ming-wei,LIAO Sen-tai,HOU Fang-li,ZHANG Rui-fen,TANG Xiao-jun,WEI Zhen-cheng,ZHANG Yan,CHI Jian-wei,DENG Yuan-yuan
    Scientia Agricultura Sinica. 2010, 43(9):  1919-1925 .  doi:10.3864/j.issn.0578-1752.2010.09.019
    Abstract ( 923 )   PDF (309KB) ( 1056 )   Save
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    【Objective】 The objective of this experiment is to study the effects of water soluble extracts from longan on immune regulation in normal mice. 【Method】 One hundred and twenty mice were randomly divided into five groups: the normal control group, low dose longan extracts (130 mg•d-1), high dose longan extracts (380 mg•d-1), low dose longan slurry (150 mg•d-1), and high dose longan slurry (450 mg•d-1). After mice were orally administered for 35 days, changes on weight, index of immune organs, indexes of spleen lymphocyte transformation, hemolysin value of blood serum, NK cell activity, plaque-forming cell response were assayed by dissolving CRBC. Besides, four anti- oxidation indicators serum malondialdehyde level, total antioxidant capacity, glutathione peroxidase and superoxide dismutase activity were also investigated. 【Result】 Compared to normal control group, both doses of longan extracts greatly improved hemolysin value of blood serum and indexes of spleen lymphocyte transformation(P<0.05) ,but the results showed no significant effect in the cases of longan slurry groups (P>0.05). And high dose longan extracts enhanced NK cell activity signifcantly (P<0.05), yet both doses of longan slurry groups showed on significant effects (P>0.05). High dose longan slurry reduced the MDA of blood serum (P<0.05), yet the longan extract groups did not show significant effects (P>0.05). T-AOC was increased by high dose longan slurry (P<0.01) and high dose longan extracts (P<0.05), respectively. 【Conclusion】It is concluded that longan extracts have a promoting effect on immune functions and antioxidation activity of normal mice, yet the anti- oxidation activity of longan is not the main mechanism for immune regulation actions.

    ANIMAL SCIENCE·RESOURCE INSECT
    In Silico Study on a New Orthologs SLC25A3 and It’s Experimental Verification in Porcine Ovary
    PAN Zeng-xiang,ZHANG Jin-bi,LIN Fei,LI Qi-fa,XIE Zhuang,LIU Hong-lin
    Scientia Agricultura Sinica. 2010, 43(9):  1926-1932 .  doi:10.3864/j.issn.0578-1752.2010.09.020
    Abstract ( 843 )   PDF (540KB) ( 798 )   Save
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    【Objective】 The objective of this study is to efficiently obtain the structural and functional information of orthologs. 【Method】 The approach of gene-oriented ESTs annotation based on model organisms, in silico analysis and the method of primers extension were combined in the study.【Result】 An orthologs SLC25A3 (Solute Carrier Family 25, Member 3)by the approach of gene- oriented ESTs annotation based on human cDNA sequences was identified, and then the homologous ESTs expressed in porcine ovary were selected for in silico cloning and experimental verification, and ulmately the structural and functional information of the orthologs SLC25A3 were obtained by bioinformatic methods.【Conclusion】 Combining the gene-oriented ESTs annotation method and the relative bioinformatcic tools, the sequene and functional information of orthologs could be obtained fastly and directly, and the transformation or communication of the valuable information between different species could be realized, thus providing reliable chioces for sequence analysis and functional annotation in comparative genomic research.

    Effects of α-lipoic Acid on Serum Fat Metabolic Parameters and Liver Enzyme Activities in the Transition Cow
    ZHU Jia-qiao,CAO Sui-zhong,XIONG Gui-lin,GU Jian-hong,WANG Ji-cang,LIU Zong-ping
    Scientia Agricultura Sinica. 2010, 43(9):  1933-1938 .  doi:10.3864/j.issn.0578-1752.2010.09.021
    Abstract ( 1007 )   PDF (287KB) ( 750 )   Save
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    【Objective】 The effects of α-lipoic acid on serum fat metabolic parameters and liver enzyme activities in the transition cow were studied in an experiment. 【Method】 Forty-eight Holstein dairy cows at 20 d before calving were divided into four groups. Group Ⅰwas used as control. Group Ⅱto Ⅳ were fed with α-lipoic acid (3, 5 or 8 g•d-1) for 50 days. Blood samples were collected before the beginning of supplementation and at intervals of 10 days after supplementation. The following parameters indicative of fat metabolism and liver enzyme activities were measured in serum: triglyceride (TG), cholesterol (Chol), nonesterified fatty acid (NEFA), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and alkaline phosphatase (AKP), γ-glutamyl transferase (γ-GT), lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). 【Result】 Serum fat metabolic parameters showed a declining trend by α-lipoic acid treatment, but only serum NEFA concentrations were significantly changed on day 30 in group Ⅳ as compared with control group (P<0.05). Serum AST activities were significantly lower on day 30 in groups Ⅱ, Ⅲ and Ⅳ than in control group (P<0.05 or P<0.01). There were no significant differences in other parameters between the treatment groups and the control. 【Conclusion】 It is concluded that α-lipoic acid supplementation can improve fat metabolism and protect liver function in the transition cow.

    VETERINARY SCIENCE
    In vitro Inhibitory Effects of Tulathromycin and Erythromycin on the Productions of NO and PGE2
    ZHOU Lei,YAN Qi-gui,WU Cong-ming
    Scientia Agricultura Sinica. 2010, 43(9):  1939-1947 .  doi:10.3864/j.issn.0578-1752.2010.09.022
    Abstract ( 864 )   PDF (419KB) ( 640 )   Save
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    【Objective】 The anti-inflammatory effects of tulathromycin were studied. 【Method】 In vitro, we make use of the model that is LPS-induced porcine peripheral blood mononuclear cells (PBMC) overexpressing pro-inflammation factors was used, and then the real-time Q-PCR was employed to study the effect of different concentrations of tulathromycin and erythromycin on the generation of NO and PGE2 involved in the inflammatory process on the molecular level. 【Result】 Results showed that tulathromycin at the concentrations of 5, 10 and 20 μg•mL-1 significantly decreased the production of NO and PGE2, a similar pattern was also observed on the cyclooxygenase (COX-2) and inducible nitric oxide synthase (iNOS) gene transcription. However, all 5, 10 and 20 μg•mL-1 of erythromycin significantly decreased the production of NO and PGE2 on the transcription and expression level. 【Conclusion】 These results support the opinion that macrolides may exert anti-inflammatory effect through modulating the synthesis of NO and PGE2 in inflammatory process.

    Effect of Soy Isoflavone on the Expression of IL-2 mRNA in the Ovariectomized Rat’s Spleen
    CHEN Zheng-li,LUO Qi-hui,CHENG An-chun
    Scientia Agricultura Sinica. 2010, 43(9):  1948-1953 .  doi:10.3864/j.issn.0578-1752.2010.09.023
    Abstract ( 903 )   PDF (429KB) ( 719 )   Save
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    【Objective】 The objective of the experiment is to research on the effects of soy isoflavone on expression of IL-2 mRNA in the spleen of ovariectomized (OVX) rat. 【Method】 Sixty young female rats were ovariectomized, and then these rats were supplied with soy isoflavone respectively in high dosage (1.5 mg•kg-1), medium dosage (1.0 mg•kg-1), low dosage (0.5 mg•kg-1) and solvent agent via hypodermic injection. At the second, fourth and sixth week after treatment, 5 rats per group were killed for study. The expression and distribution of IL-2 mRNA in the spleen were studyed by in situ histo-hybridization method. 【Result】 The IL-2 mRNA mainly distributed in red pulp close to the capsule, some in periphery of splenic corpuscle, marginal zone and outer layer of periarterial lymphatic sheath. The general tendency of the intensity and the number of IL-2 mRNA positive cells of OVX rats are significantly decreased, and the degression was more significantly with the time going after ovariectomizing treatment. While after treatment with soy isoflavone, the general tendency of the intensity and the number of IL-2 mRNA positive cells of OVX rats were increased and showed a time-dependent manner. In the same period of time, with the increasing concentration of soy isoflavone, the general tendency of the intensity and the number of IL-2 mRNA positive cells were increased and showed a dose-dependent manner. 【Conclusion】 The above results suggested that soy isoflavone could upregulate the expression of IL-2 mRNA and showed a time- and dose-dependent manner.

    RESEARCH NOTES

    Cloning and Prokaryotic Expression of an avenin-like from Wheat Variety Shaan 253

    CHEN Rui-ji,GAO Xiang,CHEN Qi-jiao,DONG Jian,ZHAO Wan-chun,LI Yan-liang,WANG Ming-xia,LI Min,PANG Hong-xi,LI Zhe-qing,LIU Jun
    Scientia Agricultura Sinica. 2010, 43(9):  1954-1962 .  doi:10.3864/j.issn.0578-1752.2010.09.024
    Abstract ( 967 )   PDF (804KB) ( 797 )   Save
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    【Objective】 Molecular cloning and sequence characterization analysis of avenin-like from wheat cultivar Shaan 253, which were then constructed their prokaryotic expression vector and expressed in Escherichia coli Rosetta-gami B (DE3). 【Method】 The novel avenin-like were amplified from wheat variety Shaan 253 by PCR and then cloned into the pMD19-T vector, one of which was subcloned into the expression vector. The recombinant plasmid was identified by sequencing and digestion of restriction enzymes. The fusion protein was finally expressed by IPTG-induction in host bacteria- E. coli Rosetta-gami B (DE3) and detected by Western blot. 【Result】 Three novel avenin-like were obtained, and submitted to GenBank, and their accession No. are GQ903577, GQ903578, and GQ903579, respectively. Sequence analysis revealed that GQ903579 was a pseudogene. The gene GQ903578 was successfully expressed in the prokaryotic expression system. 【Conclusion】 It could provide a foundation for quality improvement of wheat.

    Effects of Calcium on Photosynthesis and Chlorophyll Fluorescence of ‘Kyoho’ Grape Under High Temperature Stress
    ZHENG Qiu-ling,TAN Wei,MA Ning,ZHAI Heng
    Scientia Agricultura Sinica. 2010, 43(9):  1963-1968 .  doi:10.3864/j.issn.0578-1752.2010.09.025
    Abstract ( 1073 )   PDF (316KB) ( 1226 )   Save
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    【Objective】 In order to reveal the protection function of calcium on photosynthetic organ in leaves of ‘Kyoho’ grape under high temperature stress, the effects of CaCl2 on photosynthesis and chlorophyll fluorescence under heat stress were studied. 【Method】 During the period of high temperature, the grapes grown in the field were pretreated with CaCl2, CaCl2+Ca2+ chelator EGTA and CaCl2+CaM antagonist CPZ. Photosynthetic rate, chlorophyll fluorescence and MDA content were determined. 【Result】The net photosynthesis rate (Pn), qP, Fv’/Fm’ and Fv/Fm in the grape leaves pretreated with CaCl2 were much higher than that of the control, while Fo was much less than that of the control, and the accumulation of final product of membrane lipid peroxidation (MDA) was slightly lower than the control. EGTA and CPZ pretreatments significantly inhibited the photosythesis, Pn were 29.4% and 26.5% lower than that of CaCl2 pretreatment; the activity of PSⅡ was inhibited significantly, the accumulation of MDA were 33.7% and 40.2% higher than that of CaCl2 pretreatment. 【Conclusion】 CaCl2 pretreatment effectively alleviated the inhibition of photosynthesis in grape leaves under high temperature stress and the damage to the function of PSⅡ to some extent.

    Sequencing and Analysis of the Complete Genomic Sequence of Pineapple bacilliform comosus virus
    Scientia Agricultura Sinica. 2010, 43(9):  1969-1976 .  doi:10.3864/j.issn.0578-1752.2010.09.026
    Abstract ( 1037 )   PDF (489KB) ( 793 )   Save
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    【Objective】 Pineapple bacilliform comosus virus (PBCoV) was identified on pineapple in Zhanjiang district, Guangdong province, China, and the complete genomic sequence of PBCoV was analyzed and compared in the molecular variation among Badnaviruses. 【Method】 The specific primers of PBCoV were designed, and the viral partial and whole genomic sequences were, respectively, amplified with regular or inverse PCRs and analyzed by proper bioinformatics softwares. 【Result】 The complete circular genomic nucleotide sequence of PBCoV comprised 7 543 bp, which contained 3 ORFs on the plus-strand that potentially encoded proteins of 20.4, 14.0 and 217.6 kD, respectively. The nucleotide sequences of PBCoV shared 87%-97% identities with the partial sequences of PBCoV from Australia in GenBank. The amino acid sequence encoded by the viral ORF3 shared less than 50.8% identities with that of any known Badnaviruses. Evidence that the PBCoV sequence is integrated in the pineapple genome was presented and discussed. 【Conclusion】 The results indicated that PBCoV exists on pineapple in Zhangjiang district, Guangdong province, China. The virus belongs to Badnavirus, which shares the most identities in nucleotide level with Banana streak virus -Mys isolate. This is first to report the complete genomic sequence and structure of PBCoV.