Scientia Agricultura Sinica ›› 2024, Vol. 57 ›› Issue (20): 3998-4006.doi: 10.3864/j.issn.0578-1752.2024.20.006

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• SPECIAL FOCUS: OCCURRENCE AND CONTROL OF MIGRATORY PESTS • Previous Articles     Next Articles

Effect of Rice Varieties on the Preference of Nilaparvata lugens to Rice Plants Infested by Chilo suppressalis

CAI YuBiao(), ZHANG KunJie, WANG YaXuan, LAI FengXiang, HE JiaChun, WAN PinJun(), FU Qiang()   

  1. State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006
  • Received:2024-05-04 Accepted:2024-06-10 Online:2024-10-16 Published:2024-10-24
  • Contact: WAN PinJun, FU Qiang

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Abstract:

【Objective】It has been reported that Nilaparvata lugens (brown planthopper, BPH) exhibits a preference of endangering rice plants infested by Chilo suppressalis (striped stem borer, SSB). However, it remains unclear whether this phenomenon is influenced by the developmental stage of N. lugens and the rice variety. This study aims to investigate the effects of rice varieties and developmental stages of N. lugens on the preference for C. suppressalis-infested rice plants, as well as how volatiles mediate this behavior, so as to provide a scientific basis for formulating green pest control strategies.【Method】Through indoor choice experiments, the attraction of different developmental stages of N. lugens (including 3rd-4th instar nymphs, newly emerged females, gravid females) to nine rice varieties, including Zhongzheyou 8, both damaged and undamaged by C. suppressalis, was observed. Based on these observations, representative varieties showing significant differences in attraction were selected for further analysis. RT-qPCR was used to detect changes in the expression of volatile synthesis-related genes in three rice varieties (Zhongzheyou 8, 9311, and Minghui 63) following pest infested.【Result】Among the nine rice varieties, different developmental stages of N. lugens consistently showed a preference for rice plants infested by C. suppressalis, although this preference varied among rice varieties. For instance, newly emerged female N. lugens exhibited a significantly higher preference for Minghui 63 compared to Yongyou 538. Gene expression analysis revealed a significant increase in the expression levels of volatile synthesis-related genes OsCAS, OsLIS, OsHPL3, and OsRCI-1 in response to C. suppressalis infestation. However, when different developmental stages of N. lugens were co-infested with C. suppressalis, the expression levels of these genes could either increase or decrease compared to plants infested only by C. suppressalis, with specific effects depending on the developmental stage of N. lugens.【Conclusion】The preference of N. lugens for rice plants infested by C. suppressalis was commonly observed across the nine tested rice varieties. However, significant differences in the degree of preference were noted among different developmental stages of N. lugens across these varieties. Notably, variations in preference among developmental stages of N. lugens were primarily observed in the selection of rice varieties Yongyou 538, Zhongzheyou 8, and Minghui 63 by newly emerged female. Infestation only by C. suppressalis or in conjunction with N. lugens resulted in increased expression levels of the genes OsCAS, OsLIS, OsHPL3, and OsRCI-1, indicating that pest infestation in rice plants can regulate the synthesis of rice volatiles, thereby influencing the host selection behavior of N. lugens.

Key words: rice variety, Nilaparvata lugens, Chilo suppressalis, host preference, volatile

Table 1

The information of primers for qPCR"

基因
Gene		 上游引物序列
Upstream primer sequences (5′-3′)		 下游引物序列
Downstream primer sequences (5′-3′)		 参考文献
Reference
OsUbq GTTCGCCCAGTTGACATCTC CAGATTGTTGAGGTTAGTATTGC [18]
OsHPL3 TGGTGCCGACGCAGAAGGTG GCAGCGGGAAGGAGTGGAGC [14]
OsLIS TTTGATGGATTCATGACAGAGA CAACAAACTCTGCTGCATTTT
OsCAS CGCTACGAGATGCTTTTACAAC CACCGTAGCAGCTACCTGATC
OsRCI-1 AGGTTCAACGAGAGGATGATGAG TTCGCTCCGGATCCTTGTT [16]

Fig. 1

The preference of N. lugens to the plants infested by C. suppressalis of nine rice varieties"

Table 2

Three-way ANOVA of rice variety, N. lugens stage, and time after infestation"

方差来源Variable source 自由度df 卡方值χ2 value P值P value
水稻品种Rice variety 8 50.944 <0.0001
褐飞虱虫态N. lugens stage 2 22.978 <0.0001
接虫后时间Time after N. lugens infestation 2 0.462 0.7937
水稻品种×褐飞虱虫态Rice variety×N. lugens stage 16 47.616 0.0001
水稻品种×接虫后时间Rice variety×Time after N. lugens infestation 16 5.776 0.9903
褐飞虱虫态×接虫后时间N. lugens stage×Time after N. lugens infestation 4 1.976 0.7399
水稻品种×褐飞虱虫态×接虫后时间
Rice variety×N. lugens stage×Time after N. lugens infestation		 32 11.840 0.9996

Table 3

Variance analysis of rice variety selection by three N. lugens stages after C. suppressalis feeding at observation time points"

虫态Stage 时间Time (h) 自由度df 卡方值χ2 value P值P value
3—4龄若虫
3rd-4th instar nymph		 12 8 14.232 0.076
24 8 7.528 0.481
48 8 10.592 0.226
初羽化雌虫
Newly emerged female		 12 8 19.648 0.012
24 8 23.192 0.003
48 8 10.968 0.203
怀卵雌虫
Gravid female		 12 8 6.880 0.550
24 8 9.752 0.283
48 8 13.304 0.102

Table 4

Selection rates of newly emerged female N. lugens for different rice varieties fed by C. suppressalis (%)"

时间Time (h) 水稻品种Rice variety 均值±标准误Mean±SE
12 明恢63 Minghui 63 65.82±2.40a
秀水134 Xiushui 134 61.99±2.39ab
甬优1540 Yongyou 1540 61.53±2.21ab
9311 61.25±1.90ab
嘉禾218 Jiahe 218 60.23±1.76ab
TN1 58.04±1.97ab
天优华占Tianyouhuazhan 57.01±2.98ab
中浙优8号Zhongzheyou 8 55.77±2.70ab
甬优538 Yongyou 538 52.67±1.52b
24 明恢63 Minghui 63 66.71±2.32a
9311 62.18±1.74ab
秀水134 Xiushui 134 61.34±2.70ab
甬优1540 Yongyou 1540 60.31±2.02ab
嘉禾218 Jiahe 218 59.97±2.14ab
TN1 56.57±1.69ab
天优华占Tianyouhuazhan 56.38±3.08ab
中浙优8号Zhongzheyou 8 55.43±2.62b
甬优538 Yongyou 538 52.56±1.60b

Fig. 2

The relative expression levels of genes related to volatile synthesis in rice under different treatments Data in the figure are mean±SE. Different lowercases on the bars indicate significant differences in the gene expression levels among CK, T1, T2, T3 and T4 treatments (P<0.05, one-way ANOVA followed by Tukey’s HSD test)"

Table 5

Effect of rice variety and C. suppressalis (N. lugens) feeding on volatile-related gene expression"

方差来源Variable source OsCAS OsLIS OsHPL3 OsRCI-1
处理Treatment <0.001 <0.001 <0.001 <0.001
水稻品种Rice variety <0.001 0.292 0.017 0.466
处理×水稻品种Treatment×Rice variety <0.001 <0.001 <0.001 <0.001
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