Scientia Agricultura Sinica ›› 2023, Vol. 56 ›› Issue (13): 2597-2608.doi: 10.3864/j.issn.0578-1752.2023.13.013

• ANIMAL SCIENCE·VETERINARY SCIENCE • Previous Articles     Next Articles

Effects of Smad7-Mediated TGF-β Signaling Pathway on Proliferation of Sheep Granulosa Cells

GUO ZeYuan(), DU ZhangSheng, ZHANG YaQi, CHEN ChunLu, MA XiaoYan, CHENG Ying, WANG Kai, LÜ LiHua()   

  1. College of Animal Science, Shanxi Agricultural University, Taigu 030801, Shanxi
  • Received:2022-05-06 Accepted:2023-02-13 Online:2023-07-01 Published:2023-07-06
  • Contact: Lü LiHua

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Abstract:

【Background】Follicle development is a complex regulatory process, in which the proliferation and differentiation of ovine follicular granulosa cells (Granulosa Cells, GCs) will directly affect the development of follicles. TGF-β signaling pathway plays an important role in sheep ovarian development and follicle growth, and Smad7 also plays an important role as a key inhibitor of TGF-β signaling pathway. 【Objective】In this study, the effect of Smad7-mediated TGF-β signaling pathway on the proliferation and apoptosis of ovine follicular GCs was explored to provide a basis for further research on the regulatory role of Smad7 in the process of follicular GCs proliferation and apoptosis. 【Method】20 non-pregnant Jinzhong Duhu hybrid ewes aged 4-6 months were selected. After slaughtering, bilateral ovaries were collected, and GCs were isolated and cultured in vitro. And then, FSHR cell and expression localization of Smad7 cells was identified by immunofluorescence, the cell proliferation was measured by CCK8 method, and the growth curve was drawn. After the cells were passaged, different concentrations of Smad7 agonist Asiaticoside AS (0, 100, 200, 400, 600 ng·mL-1) were added exogenously, and the optimal concentration of AS was selected to treat the second generation GCs. The expression levels of key genes as well as apoptosis and cycle-related genes in the TGF-β signaling pathway were detected using qRT-PCR and Western blotting. Three siSmad7s were synthesized and transfected into follicular GCs, and the one with the best interference effect was selected. The expression levels of key genes in the TGF-β signaling pathway, apoptosis and cycle-related genes were detected by qRT-PCR and Western blotting. 【Result】Smad7 was expressed in ovine follicular GCs; 200 ng·mL-1 AS could significantly up-regulate the expression of Smad7 in GCs (P<0.01), siSmad7-1 could effectively transfected ovine follicle GCs (P<0.01); Up-regulation of Smad7 could significantly increase the expressions of Smad4, TFDP-1 and EP300 in the TGF-β signaling pathway (P<0.05), extremely significantly increase the expression of SP1 in the TGF-β signaling pathway (P<0.01), and significantly increase the expression of apoptosis-related gene Caspase8 (P<0.05), extremely significantly increased the expressions of apoptosis-related genes Caspase3 and Bim (P<0.01), significantly increased the expressions of cell cycle-related genes P21 and P27 (P<0.05), extremely significantly increased the expression of cell cycle-related gene CCND2 (P<0.01), while the expression of CCND1 was significantly decreased (P<0.05); Smad7 siRNA significantly decreased the expressions of Smad4, TFDP-1 and EP300 in the TGF-β signaling pathway (P<0.05), extremely significantly decreased the expression of SP1 in the TGF-β signaling pathway (P<0.01), and significantly decreased the apoptosis-related gene Caspase8 (P<0.05), extremely significantly decreased the expressions of apoptosis-related genes Caspase3 and Bim (P<0.01), extremely significantly decreased the expressions of cell cycle-related genes P21, P27 and CCND2 (P<0.01), extremely significantly increased the expression of CCND1 (P<0.01); CCK8 analysis demonstrated into that when Smad7 was up-regulated, the proliferation rate of GCs was significantly down-regulated at 24 h and 48 h (P<0.01). Smad7 siRNA significantly increased the proliferation rate of GCs at 24 h and 72 h (P<0.05), which also extremely significantly increased the proliferation rate of GCs (P<0.01).【Conclusion】In summary, it was suggested that Smad7 mediated TGF-β signaling pathway to inhibit the proliferation of ovine follicular GCs and to promote cell apoptosis.

Key words: asiaticoside (AS), siRNA, Smad7, TGF-β signaling pathway, sheep, granulosa cells

Table 1

Primer for Quantitative Real-time PCR"

基因 Gene 引物序列 Primer sequence (5´→3´) 长度 Product size (bp)
Smad7 F:CCCATCACCTTAGCCGACTC 101
Smad7 R:CACAGCATCTGGACAGTCAGT 101
β-actin F:GCAAAGACCTCTACGCCAAC 90
β-actin R:GGGCAGTGATCTCTTTCTGC 90
TFDP1 F:GTGGTCGCGGGAAAACCA 175
TFDP1 R:GACAGCAACCAAGGACACCA 175
SP1 F:GCTACCATGAGCGACCAA 101
SP1 R:CTACCATTTCCATTGCCCCC 101
EP300 F:CCAGCCTCTTAAGATGGGAAT 110
EP300 R:TCGGAATCTGGAGACCAAGG 110
Smad4 F:GAGCAAGGTTGCACATAGGC 80
Smad4 R:GTCACTAAGGCACCTGACCC 80
CCND1 F:TGATCAGATGTGACCCGGACT 185
CCND1 R:CCCTCAAATGTTCACGTCGC 185
CCND2 F:AGACCTTCATCGCTCTGTGC 186
CCND2 R:TGAGGCAATCCACATCCGTG 186
P21 F:GCTTCTTGTACCAGGACCCAG 118
P21 R:CAGAGAGTTCGCTTCACCGAG 118
P27 F:ACCACCGTCAAACCTTACTCC 159
P27 R:TTCTCTGTAGGACCCCTCGT 159
Caspase3 F:CCCACTGCAGCAACATTAATCC 252
Caspase3 R:CGACAGGCCATGCCAGTATT 252
Caspase8 F:ACTGTGTGGAGCAGGTAACA 128
Caspase8 R:CCGGCTTAGGAACTTGAGGG 128
Bim F:TGAGGCAGTCTCAGGCTGTA 153
Bim R:GGAGGACCATTTGTGGGTGG 153

Fig. 1

Immunofluorescence identification of ovine GCs"

Fig. 2

Immunofluorescence localization of Smad7 in ovine GCs"

Fig. 3

Transfection of Smad7 agonist and siRNA in ovine follicular GCs *means significant difference, P<0.05, and ** means extremely significant difference, P<0.01. The same as below"

Fig. 4

Effects of 200 ng·mL-1 AS on the expression of Smad7 protein in ovine follicular GCs"

Fig. 5

Effects of siSmad7-1 treatment on the expression of Smad7 protein in ovine follicular GCs"

Fig. 6

The effect of Smad7 on the expression levels of related genes in TGF-β signaling pathway"

Fig. 7

Effects of Smad7 up-regulation and down-regulation on the proliferation rate of ovine follicular GCs"

Fig. 8

Effects of Smad7 up-regulation and down-regulation on cell cycle-related genes of ovine follicular GCs"

Fig. 9

Effects of Smad7 on apoptosis-related genes of ovine follicular GCs"

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