普通小麦-十倍体长穗偃麦草衍生新品种抗赤霉病基因的分子鉴别

doi:10.3864/j.issn.0578-1752.2017.20.007

Abstract

Abstract: 【Objective】 Fusarium head blight (FHB) is one of the most devastating diseases of wheat. Thinopyrum ponticum is one of the important source resistance cultivars of wheat disease resistance breeding, with high tolerance to FHB. Three derived varieties of common wheat-Th. ponticum Xinong 509, Xinong 511 and Xinong 529 showed moderate resistance to FHB in field. The objective of this study is to clarify the genetic constitution of resistance gene in three varieties.【Method】 Single drop injection with Fusarium graminearum was performed to detect the reaction of the three derived varieties of common wheat-Th. ponticum to FHB. Specific molecular markers located on homologous group 1 to 7 of Thinopyrum E chromosome were employed to detect the sources of resistance to FHB, screening the three derived varieties of common wheat-Th. ponticum, and their major donor parents Xiaoyan 693, Xiaoyan 597 and Th. ponticum. Specific molecular markers closely linked with ?anking Fhb7 were used to analyze the relationship between the gene conferring resistance to FHB and Fhb7.【Result】 Three derived varieties were moderately resistant to FHB in the field experiment, and had no much difference compared with the medium-resistant control variety. Seven of the 105 chromosome E specific molecular markers of Thinopyrum could be amplified polymorphism bands in the three derived varieties and Th. ponticum, which located on the chromosome 7E. A total of 97 specific markers located on the chromosome 7E,20 showed specific bands of Th. ponticum in the three derived varieties of /span>common wheat-Th. ponticum, and their major donor parents Xiaoyan 693, Xiaoyan 597. The results indicate that the 89 cM genetic fragment derived from Th. ponticum 7E chromosome. Of them, six molecular markers (XsdauK8, XsdauK144, XsdauK27, XsdauK99, Xcfa2040 and XsdauK116), which were located on 7EL 149.00-7EL 153.77, tightly linked to Fhb7. However, XsdauK60 (7EL 153.77), Xmag1932 (7EL 154.70), Xcfa2240 (7EL 156.27), XsdauK66 (7EL 158.02), XsdauK71 (7EL 158.97) and Xsews19 (7EL 160.00),which are next to Fhb7, showed no specific bands ofTh. ponticum among the three derived varieties of common wheat-Th. ponticum and Xiaoyan 597. 【Conclusion】 Xinong 509, Xinong 511 and Xinong 529, derived from Th. ponticum with good FHB resistance, possess a novel FHB resistance gene, which derived from Th. ponticum and was different from Fhb7.

Key words: common wheat, Thinopyrum ponticum;Fusarium head blight, Fhb7, molecular marker

LIU XinLun, WANG Chao, NIU LiHua, LIU ZhiLi, ZHANG LuDe, CHEN ChunHuan, ZHANG RongQi, ZHANG Hong, WANG ChangYou, WANG YaJuan, TIAN ZengRong, JI WanQuan. Molecular identification of FHB resistance gene in varieties derived from common wheat-Thinopyrum ponticum partial amphiploid[J].Scientia Agricultura Sinica, 2017, 50(20): 3908-3917.

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