新疆褐牛基因外显子多态性及其与SCS和泌乳性状关联性分析

doi:10.3864/j.issn.0578-1752.2017.13.017

Abstract

Abstract: 【Objective】The association between polymorphisms of ERAP2, Cwc27, PPFIBP2, THADA, ZNF804B and AMHR2 genes and somatic cell score (SCS) and milk production traits in Xinjiang brown cattle were studied in order to find out molecular markers association with SCS and/or milk production traits.【Method】Taking 169 Xinjiang brown cattle cows as experimental animals from Xinjiang Urumqi cattle farm and Xinjiang Tianshan Animal Husbandry and Biotechnology Co., Ltd. The experimental data including Dairy Herd Improvement records (including milk fat percentage, milk protein percentage, lactose percentage, total solid content and SCS) and milk yield of 305d, 10 SNPs were selected from exon area based on the results of DNA sequencing of 15 Chinese Holstein cows in an initial study and genotyped by Sequenom MassARRAY Genotype technology, the association between SNPs or haplotype and SCS and milk yield traits were analyzed by the least squares method in the GLM procedure of SAS8.1.【Result】The results showed that the 10 SNPs were polymorphic, and 9 SNPs were in Hardy-Weinberg equilibrium. Association analysis results showed that two SNPs in ERAP2 gene (T98741711C and G98736141A) had a highly significant association with milk yield of 305d in Xinjiang brown cattle(P＜0.001), the milk yield of TT and GG genotype was the highest in T98741711C locus and G98736141A locus, respectively; C45667492G locus in PPFIBP2 gene had a significant association with milk lactose percentage (P＜0.005), GG genotype was the highest; Cwc27 (T14533269A) had a significant association with milk fat percentage and total solid content(P＜0.005) and had a highly significant association with SCS(P＜0.001), milk fat percentage of TT genotype was the highest and SCS of AA genotype was higher than AT and TT genotypes; AMHR2 (C26758055G) had a highly significant association with milk protein percentage and total solid content(P＜0.001), GG genotype was higher than CC and GC genotypes. The results of linkage disequilibrium analysis and haplotype construction revealed that the 10 SNPs constructed two haplotype blocks, among them, SNP1 and SNP2 were in linkage disequilibrium state(0.3＜r²＜0.6), SNP4 and SNP10 in strong linkage disequilibrium state(r²＞0.6). Then the correlation between haplotype and SCS and/or milk production traits was analyzed, and the results showed that haplotype had no significant association with SCS and milk production traits(P＞0.001).【Conclusion】In this study, it was found that ERAP2, Cwc27, PPFIBP2 and AMHR2 genes had a significant association with SCS and milk production traits, and the results of the study will provide a theoretical basis and reference for the molecular marker-assisted selection in Xinjiang Brown Cattle.

Key words: Xinjiang Brown Cattle, SCS, milk production traits, MassArray, association analysi

LIU LiYuan, ZHOU JingHang, ZHANG MengHua, LI JinXia, FANG JiQing, TAN ShiXin, WANG AiFang, HUANG XiXia, WANG YaChun. Genetic Effect Analysis of SNPs from 6 Genes on SCS and Milk Production Traits in Xinjiang Brown Cattle[J].Scientia Agricultura Sinica, 2017, 50(13): 2592-2603.

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Genetic Effect Analysis of SNPs from 6 Genes on SCS and Milk Production Traits in Xinjiang Brown Cattle

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