Scientia Agricultura Sinica ›› 2015, Vol. 48 ›› Issue (6): 1228-1239.doi: 10.3864/j.issn.0578-1752.2015.06.18

• RESEARCH NOTES • Previous Articles     Next Articles

Identification of Resistance to Main Virus Diseases and Genetic Diversity Study of Tobacco Foundation Parents

DAI Shuai-shuai, REN Min, JIANG Cai-hong, CHENG Ya-zeng, GENG Rui-mei, ZHANG Xing-wei, CHENG Li-rui, YANG Ai-guo   

  1. Key Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, Shandong
  • Received:2014-09-21 Online:2015-03-16 Published:2015-03-16

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Abstract: 【Objective】 This study aims to assess the viral disease resistances of the tobacco foundation parents, and to analyze their genetic diversity and phylogenetic relationship. 【Method】 A total of 73 flue-cured tobacco foundation parents were evaluated for resistances to three kinds of virus diseases by artificial inoculation in the green house. Furthermore, the genetic diversity of 29 resistant foundation parents and additional 4 foundation parents were analyzed using 103 pairs of polymorphism SSR markers distributed on the whole tobacco genome. 【Result】 Different cultivars displayed a wide range of variation for resistances to CMV, TMV and PVY. As for CMV, a total of 23 cultivars exhibited high resistances to CMV, although the cultivars exhibited immunity to CMV were not identified. As for TMV, six cultivars exhibited immunity to TMV. As for PVY, five cultivars exhibited immunity to PVY. In sum, two cultivars, Longyan1 and FC8, exhibited immunity to TMV and PVY, respectively. In addition, seven cultivars including CV87, T.T.11, Kang88, CV91, Tailifu1060, Gexin5 and T.I.245, exhibited resistances to three kinds of virus diseases. The genotypes of 29 resistant foundation parents and additional 4 foundation parents were identified with 103 SSR markers. The total numbers of alleles amplified at the 103 SSR loci were 322 and the mean number of alleles detected for each locus was 3.106, ranging from 2 to 6 alleles. The genetic diversity spanned from 0.059 to 0.716, with an average of 0.387. The PIC value ranged from 0.057 to 0.661, with an average of 0.343. According to UPGMA cluster analysis, the 33 tested cultivars were divided into seven clusters with a cut-off of threshold value as 0.672 and the cultivars belonged to the first group were further subdivided into four clusters with a cut-off of threshold value as 0.714. Principle coordinate analysis (PCoA) showed that the 33 tested cultivars were divided into six clusters, which are in agreement with the results of clustering analysis. The results of molecular clustering largely agreed with the pedigree relationship.【Conclusion】 A total of 29 flue-cured tobacco foundation parents exhibited the resistance to tobacco main virus diseases. The genetic relationships among the groups of genotyped cultivars are in agreement with pedigree relationship. The resistant foundation parents from China showed a relatively higher genetic basis.

Key words: tobacco, CMV, TMV, PVY, resistance to disease, genetic diversity, cluster analysis

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