Scientia Agricultura Sinica ›› 2015, Vol. 48 ›› Issue (12): 2287-2295.doi: 10.3864/j.issn.0578-1752.2015.12.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Fine Mapping of Rice Panicle Apical Abortion Gene qPAA3 Interacting with SP1

ZHANG Xing-yuan1, LUO Sheng2, WANG Min2, CONG Nan2, ZHAO Zhi-chao2, CHENG Zhi-jun2   

  1. 1Liu’An Vocational Technical College, Liu’An 237158, Anhui
    2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081
  • Received:2014-12-30 Online:2015-06-16 Published:2015-06-16

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Abstract: 【Objective】 Rice spikelet degradation reduces branch number and total grain number of single panicle, which seriously cause the yield loss per plant, is a harmful character of rice. Because of the complex genetic basis, together frequently influenced by the environment factors, up to now, there is few report about panicle apical abortion-related gene isolation. Fine mapping of panicle apical abortion mutant is meaningful in understanding of the molecular mechanism underlying panicle apical abortion formation and in the field of molecular breeding.【Method】In the present study, SP1 was mapped using the two F2 populations derived from the crosses between sp and ITA182, sp and J160, respectively. qPAA3 was finely mapped using the BC1F2 sub-population numbered 14C2017 with a pseudo-double-mutant as the non-recurrent parent originated from the cross between sp and a panicle apical abortion line 05261.【Result】 Genetic analysis showed that the sp was determinated by a single recessive nuclear gene. The candidate gene SP(t)was primarily linked on Chr.11 with the SSR markers of RM26281 and RM7391. Using the newly developed 60 markers, the SP(t) gene was further mapped between sc50 and sc66, with the genetic distance of 0.29 cM and 0.23 cM, respectively, and the physical distance 229 kb. Finally, the SP(t)gene was finely mapped between sc24 and sc66 with the physical distance of 54.3 kb. The sequencing results showed that there was 15.03 kb deletion within this region in mutant. The candidate gene was identified as a well-known gene SP1. Then, through analysis on the phenotype of double mutants combining bt and sp, it was found that there is an interaction between one of panicle apical abortion QTLs and SP1. Over consecutive 11 generation of self-crossing, the pseudo-double-mutant was further used as recurrent backcross parent to construct 81 BC1F2 sub-populations. Linkage analysis on 184 individuals from the sub-population 14C2017 by 114 polymorphic SSR primers revealed that, one QTL, within the fragment of 97.3 kb delimited by the SSR markers of RM6929 and RM1319, was corresponding to the previously documented QTL-qPAA3. 【Conclusion】The small panicle mutant sp was caused by the deletion of candidate gene SP1,interacting with one of the panicle apical abortion QTLs from bt. The later was qPAA3 and finally delimited to the physical distance of 97.3 kb by SSR marker RM6929 and RM1319 on the chromosome 3.

Key words: rice, panicle, apical abortion, QTL, fine mapping

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