华南大豆种质对大豆疫霉根腐病的抗性分析

doi:10.3864/j.issn.0578-1752.2015.12.002

Abstract

Abstract: 【Objective】 Phytophthora root rot caused by Phytophthora sojae is a destructive disease of soybean in most soybean-growing regions throughout the world. Development of Phytophthora-resistant varieties is the most economical and effective way to control this disease. The aim of this study was to screen accessions with resistance to strain PGD1 and multiple Phytophthora-resistant accessions, and to analyze resistance genes of soybean accessions from South China to phytophthora root rot. 【Method】The 631 soybean accessions mainly from six provinces (Guangdong, Guangxi, Fujian, Hainan, Hunan, Jiangxi and Sichuan) were evaluated with strain PGD1 using the hypocotyl inoculation method with minor modifications. And eighty-three Phytophthora-resistant accessions to strain PGD1 were evaluated with other six strains. 【Result】One hundred and one accessions were resistant to strain PGD1 with the ratio of 16.0%, 11.6% accessions had the intermediate response and 72.4% were susceptible to strain PGD1. Eighty-three Phytophthora-resistant accessions to strain PGD1 were identified with Pm14, Pm28, PNJ1, PNJ3, PNJ4, and P6497, and the infection rates were 28.9%, 34.9%, 9.6%, 66.3%, 57.8%, and 10.8%, respectively. Four of 83 soybean accessions were resistant to seven strains which were ZDD21538, ZDD21604, ZDD14286 and Mingxiadou1. Overall, 18.1% of the accessions were resistant or intermediately resistant to seven strains (virulence frequency 0). Eighty-three soybean accessions elicited 20 different reaction types with the 7 strains of pathogen. One reaction type of 8 accessions was consistent with Williams79 carrying a single gene. Nine reaction types of 45 accessions were consistent with two-gene or three-gene combinations, and they were deduced that carry two-gene or three-gene combinations. Thirty-eight accessions in 11 new reaction types probably contain new Rps genes. 【Conclusion】These results revealed the availability of strain PGD1 resistant accessions and showed an availability of multiple Phytophthora-resistant accessions in South China. These Phytophthora-resistant accessions can be used for an important parent soybean resistance breeding in tropical and subtropical regions and mapping resistant gene in soybean.

Key words: soybean, phytophthora root rot, resistance gene, gene postulation

CHENG Yan-bo, MA Qi-bin, MU Ying-hui, TAN Zhi-yuan, WU Hong, NIAN Hai. Analysis of Resistance Genes of Soybean Accessions from South China to Phytophthora Root Rot[J].Scientia Agricultura Sinica, 2015, 48(12): 2296-2305.

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