Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (20): 4045-4055.doi: 10.3864/j.issn.0578-1752.2014.20.013

• HORTICULTURE • Previous Articles     Next Articles

Comparison Between Tetraploid Watermelon(Citrullus lanatus) and Its Diploid Progenitor of DNA Methylation Under NaCl Stress

ZHU Hong-ju, LIU Wen-ge, ZHAO Sheng-jie, LU Xu-qiang, HE Nan, DOU Jun-ling, GAO Lei   

  1. Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009
  • Received:2014-03-04 Revised:2014-06-09 Online:2014-10-16 Published:2014-10-16

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Abstract: 【Objective】 The morphological differences and DNA methylation changes of diploid and tetraploid watermelon seedlings after NaCl stress were studied, combined with morphological indicators, this experiment aimed at explaining the resistance mechanisms between diploid and tetraploid watermelon seedlings from the perspective of epigenetics.【Method】Three groups of different ploidy (diploid, tetraploid) watermelon seedlings were used in this experiment, the watermelon seedlings were treated by using 1/2 Hoagland nutrient solution with NaCl concentration at 0, 100, 200, 300, and 400 mmol.L-1 when the seedlings had three euphyllas, after 8 days of NaCl treatment, the salt stress index of watermelon seedlings, the changes of methylation levels and patterns of the watermelon seedlings were analyzed by methylation-sensitive amplified polymorphism (methylation sensitive amplified polymorphism, MSAP) technology.【Result】 According the salt-damage morphological index comparison, it was found that after NaCl treatment, with the increasing NaCl concentration, the extent of injury of three groups watermelon seedlings also increased. There was a difference in degree of injury among three watermelon varieties, but at the same NaCl concentration, the degree of injury in the diploid watermelon seedlings was greater than tetraploid which was in the same variety. From the methylation level perspective, the whole genomic DNA methylation rate, half rate of methylation, the total rate of methylation of watermelon seedlings decreased with the increase of NaCl concentration, at the same NaCl concentration level, the methylation rate of diploid watermelon seedlings was bigger than the tetraploid ones, the methylation level was positively correlated with the degree of injury of the watermelon seedlings. From the methylation patterns, the DNA demethylation ratio decreased and the hypermethylation rate increased after NaCl stress, at the same time, the magnitude of the changes in the tetraploid watermelon seedlings was greater than the diploid ones, the methylation patterns of watermelon seedlings were negatively correlated with their degree of injury.【Conclusion】 The genomic DNA methylation status of watermelon seedlings was changed after NaCl stress, and the changes showed a high linear correlation with the NaCl stress degree. Watermelon seedlings got more tolerance to NaCl stress by reducing methylation rate and the methylation ratio, and the tetraploid watermelon seedlings showed stronger resistance than the diploid ones through decreasing the levels of methylation and methylation ratio.

Key words: watermelon, NaCl stress, diploid, tetraploid, DNA methylation

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