Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (20): 4036-4044.doi: 10.3864/j.issn.0578-1752.2014.20.012

• HORTICULTURE • Previous Articles     Next Articles

QTL Mapping and Epistatic QTL for Clubroot Resistance Using a Chinese Cabbage×Turnip F2 Population

LI Peng-peng1, LIANG Shan1, CHEN Bing1, YU Sha1, ZHANG Chun-yu2, SI Long-ting1, PIAO Zhong-yun1   

  1. 1College of Horticulture, Shenyang Agricultural University, Shenyang 110866
    2College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070
  • Received:2014-03-19 Revised:2014-07-18 Online:2014-10-16 Published:2014-10-16

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Abstract: 【Objective】 This study was focused on the identification of quantitative trait loci (QTL) and epistatic interaction for clubroot resistance in Brassica rapa. 【Method】 A Chinese cabbage inbred line ‘BJN’ (B. rapa ssp. pekinensis) as a maternal parent was cross-pollinated with a turnip inbred line ‘Siloga’ (B. rapa ssp. rapifera) to obtain F1 seeds. An F2 population consisted of 140 individuals from one bud self-pollinated F1 plant was employed to construct genetic map. Ninety five F2 individuals and their F3 families were used for QTL mapping and epistatic analysis for clubroot resistance (CR). In addition to 22 markers linked to 7 previously mapped CR genes, 1214 public molecular markers developed in B. rapa were scanned for polymorphism between two parents. JoinMap 4.0 was employed to construct the genetic linkage map. Clubroot resistance test was carried out against the two parental lines and F2:3 families with the field isolates of Plasmodiophora brassicae collected from Brassica napus milpa in Anhui’s Huangshan. The disease index (DI) was evaluated as the mean DI for each F2 individual based on the results of F3 seedlings. QTL detection was performed using the composite interval mapping function provided by Windows QTL Cartographer 2.5. QTL Network 2.0 was used to identify putative epistatic loci based on a mixed linear model. The association between the genotypes of CR QTL linked markers in an F2 population and the mean DI value of each F2 plant was analyzed with SPSS 18.0.0. Two-way analysis of variance (ANOVA) was used to study the interaction effects between CR QTL linked markers. The differences among the nine genotypic groups classified by two QTL linked markers (sau_um026 and BrID90197) were assessed by multiple comparisons with least significant difference test and Student-Newman-Keuls test. 【Result】 Clubroot resistance test showed that ‘Siloga’ was resistant to clubroot disease, while ‘BJN’ was susceptible. The skewed normal distribution of the disease index for clubroot disease in F2:3 families indicated that clubroot resistance was controlled by a few major genes and multiple genes as quantitative traits. Among 266 polymorphic markers detected, 222 were mapped to 10 linkage groups with a total length of 1152.6 cM. The genetic map covered 88.6% of the Chinese cabbage reference genome, and contained 5 markers linked to 3 different CR genes (CRb, Crr1 and Crr2). Two QTLs for clubroot resistance were detected in ‘Siloga’. One major QTL (qPbBa3.1) was located on the chromosome A3, and accounted for 19.02% of the phenotypic variance. Another QTL (qPbBa8.1) with minor effect was distributed on A8, and explained 7.82% of the phenotypic variance. Based on the physical location of CR QTL linked markers on the reference genome, two CR genes CRa and CRb were found in the qPbBa3.1 region, qPbBa8.1 was adjacent to Crr1. In addition, one pair of additive × additive epistasis was found between qPbBa3.1 and qPbBa8.1 with a contribution rate of 6.58%. Two-way ANOVA analysis showed that there was a significant difference between sau_um026 and BrID90197 (P=7.22×10-5), confirming the presence of epistatic effects between qPbBa3.1 and qPbBa8.1. One-way ANOVA analysis revealed a significant difference among the nine genotypic groups regarding to clubroot resistance (P=9.45×10-10). Multiple comparisons showed that the groups with the homozygous genotype as the resistant parent ‘Siloga’ at the qPbBa3.1 locus were significantly different from other groups. The groups with heterozygous genotype were also significantly different from the groups that were homozygous as the susceptible parent ‘BJN’ at qPbBa3.1. The clubroot resistance could be improved by the presence of the qPbBa8.1 locus. 【Conclusion】 The clubroot resistance was controlled by a major qPbBa3.1 and a minor qPbBa8.1, and the epistatic interactions between them in ‘Siloga’.

Key words: Brassica rapa, clubroot disease, QTL mapping, epistatic interaction

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