苦瓜水提物对拘束应激小鼠脂代谢紊乱的改善作用

doi:10.3864/j.issn.0578-1752.2014.16.015

Abstract

Abstract: 【Objective】The objective of this experiment is to study the effect of Momordica charantia fruit aqueous extract（MCFE） on lipids metabolism and oxidative situation in restraint mice and provide the theoretical basis for revealing the improving effect of M. charantia on lipids metabolism disorder.【Method】The 6 weeks old female mice were randomly divided into 5 groups including normal control group (NC) , stress control group (Mod), three MCFE groups (250 mg•kg-1, 500 mg•kg-1 and 750 mg•kg-1), which were administered samples once a day successively for 7 days. After intravenously injected 10% intralipids into the 20 h restrained mice, the content of serum lipid, lipase of mesenteric adipose tissue (MAT) and lipoprotein lipase (LPL) activity of different tissues were determined. The oxidative situation and the antioxidant capacity of the body were measured. 【Result】 The content of TG in Mod was 1.27 times of NC, but the content of low, medium and high MCFE groups were 0.63, 0.57, 0.55 times of NC. MCFE accelerated the elimination rate of TG（P<0.05）in a dose-dependent manner, and improved the fatty acid metabolism. Lipase activity in Mod was 0.77 times of NC, and low, medium and high MCFE groups were 0.77, 0.98, 1.06 times of NC. Lipase activity of medium and high MCFE groups basically reached normal level. MCFE improved lipase activity of mesenteric adipose tissue（MAT）. Compared with the Mod, the LPL activity of serum and MAT in each MCFE group increased significantly with dose-dependent（P<0.05）. But there was no significant effect on muscle LPL activity. The content of MDA and ORAC in Mod was 1.37 and 0.65 times of NC, respectively. The content of MDA of MCFE groups was lower than Mod significantly and the medium, high MCFE groups reached normal. The ORAC of MCFE groups was higher than Mod（P <0.05）. MCFE increased the antioxidant capacity of serum（ORAC）.【Conclusion】 MCFE improved the lipid metabolic dysfunction in restraint mice. The mechanism of M. charantia improves the lipid metabolic dysfunction in restraint mice may be the amelioration of oxidative situation in body and improving the LPL and LIPASE activities of related tissues.

Key words: Momordica charantia , aqueous extract , lipid metabolism , oxidative stress , mice

TANG Qin, DENG Yuan-yuan, ZHANG Rui-fen, ZHANG Yan, ZHANG Ming-wei, WEI Zhen-cheng, TANG Xiao-jun, LIU Lei, TI Hui-hui, MA Yong-xuan. Effect of Momordica charantia Fruit Aqueous Extract on Serum Lipids Metabolic Disorder in Restraint Mice[J].Scientia Agricultura Sinica, 2014, 47(16): 3300-3307.

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Effect of Momordica charantia Fruit Aqueous Extract on Serum Lipids Metabolic Disorder in Restraint Mice

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