Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (6): 1272-1279.doi: 10.3864/j.issn.0578-1752.2013.06.022

• VETERINARY SCIENCE • Previous Articles     Next Articles

Study on Induction of Porcine Putative Embryonic Germ Cells into Nerve Cells in vitro

 CONG  Yi-Mei, MA  Jing, SUN  Rui-Zhen, WANG  Jian-Yu, XUE  Bing-Hua, HU  Kui, YIN  Zhi, LIU  Zhong-Hua   

  1. College of Life Science, Northeast Agricultural University of China, Harbin 150030
  • Received:2012-10-31 Online:2013-03-15 Published:2013-02-07

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Abstract: 【Objective】The objective of the study is to detect the potential of porcine putative embryonic germ cells differentiating into nerve cells. 【Method】Co-culture of Gonad-mesonephros (GM) region stromal cells and primordial germ cells was used to get porcine putative embryonic germ cells. Directional differentiation method was adopted for neural differentiation. 【Result】 Compared with MEF , GM region stromal cells as feeder could support the growth of porcine putative embryonic germ cells with no significant difference in the proliferation of embryonic germ cells. Porcine putative embryonic germ cells had strong AP activity, Q-PCR results indicated the expression of Oct4, Sox2 and Nanog genes. Proliferating ability of porcine putative embryonic germ cells showed a "S" type, namely the growth of latency, logarithmic phase and plateau phase. Embryoid body could be derived after suspending culture of porcine putative embryonic germ cells in vitro. After induction, porcine putative embryonic germ cells could differentiate into a variety of neural cell types with expression of neural stem cells, neurons and glial cells markers.【Conclusion】These results indicate that porcine putative embryonic germ cells can be derived from porcine early gonadal and have the ability of differentiating into a variety of neural lineage cells in vitro.

Key words: putative embryonic germ cells , induce in vitro , nerve cells , pig

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