Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (2): 433-440.doi: 10.3864/j.issn.0578-1752.2013.02.023

• RESEARCH NOTES • Previous Articles    

Effect of Soil Compaction Stress on Ascorbate-Gluthione Cycle in Cucumber Plants

 ZHENG  Jun-Xian, SUN  Yan, HAN  Shou-Kun, ZHANG  Hao, WANG  Yi-Quan   

  1. 1.College of Horticulture, Northwest A & F University, Yangling 712100, Shannxi
    2.College of Natural Resources and Environment Science, Northwest A & F University, Yangling 712100, Shannxi
  • Received:2011-09-29 Online:2013-01-15 Published:2012-12-27

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Abstract: 【Objective】The objective of this study is to explore the injury mechanism of soil compaction stress to cucumber plants, and to offer a reference for sustainable utilization of soil.【Method】 Ascorbate-gluthione cycle was assayed in leaves and fine-roots of cucumber plant, ‘Jinchun 4’, growing under 1.2 or 1.55 g•cm-3 in bulk density. 【Results】 The results showed that the content of H2O2 and MDA in both leaf and root increased significantly under soil compaction stress. Furthermore, the increase amplitude in H2O2 and MDA contents was higher in the root than that in the leaf, indicating that the root was damaged more pronouncedly than the leaf by soil compaction stress. Under soil compaction stress, AsA, AsA+DHA, and GSH contents and AsA/ DHA ratio decreased significantly whereas DHA and GSSG contents and GSSG/GSH ratio increased significantly in both the leaves and the roots. GSSG+GSH contents hardly changed. The above results suggest that AsA and GSH were consumed to scavenge H2O2. In addition, the soil compaction stress enhanced the activities of APX, MDHAR, DHAR, and GR in both leaf and root, and APX activity was larger than those three enzymes, which imply that the capacity of AsA oxidation by APX to scavenge H2O2 was higher than that of re-production of AsA by MDHAR and DHAR and the reduction of GSSG to GSH by GR.【Conclusion】AsA-GSH cycle was enhanced in cucumber plants under soil compaction stress, the injury of soil compaction stress to cucumber plants was lowered.

Key words: cucumber , soil compaction stress , ascorbate-glutathione cycle

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