Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (2): 433-440.doi: 10.3864/j.issn.0578-1752.2013.02.023

• RESEARCH NOTES • Previous Articles    

Effect of Soil Compaction Stress on Ascorbate-Gluthione Cycle in Cucumber Plants

 ZHENG  Jun-Xian, SUN  Yan, HAN  Shou-Kun, ZHANG  Hao, WANG  Yi-Quan   

  1. 1.College of Horticulture, Northwest A & F University, Yangling 712100, Shannxi
    2.College of Natural Resources and Environment Science, Northwest A & F University, Yangling 712100, Shannxi
  • Received:2011-09-29 Online:2013-01-15 Published:2012-12-27

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Abstract: 【Objective】The objective of this study is to explore the injury mechanism of soil compaction stress to cucumber plants, and to offer a reference for sustainable utilization of soil.【Method】 Ascorbate-gluthione cycle was assayed in leaves and fine-roots of cucumber plant, ‘Jinchun 4’, growing under 1.2 or 1.55 g•cm-3 in bulk density. 【Results】 The results showed that the content of H2O2 and MDA in both leaf and root increased significantly under soil compaction stress. Furthermore, the increase amplitude in H2O2 and MDA contents was higher in the root than that in the leaf, indicating that the root was damaged more pronouncedly than the leaf by soil compaction stress. Under soil compaction stress, AsA, AsA+DHA, and GSH contents and AsA/ DHA ratio decreased significantly whereas DHA and GSSG contents and GSSG/GSH ratio increased significantly in both the leaves and the roots. GSSG+GSH contents hardly changed. The above results suggest that AsA and GSH were consumed to scavenge H2O2. In addition, the soil compaction stress enhanced the activities of APX, MDHAR, DHAR, and GR in both leaf and root, and APX activity was larger than those three enzymes, which imply that the capacity of AsA oxidation by APX to scavenge H2O2 was higher than that of re-production of AsA by MDHAR and DHAR and the reduction of GSSG to GSH by GR.【Conclusion】AsA-GSH cycle was enhanced in cucumber plants under soil compaction stress, the injury of soil compaction stress to cucumber plants was lowered.

Key words: cucumber , soil compaction stress , ascorbate-glutathione cycle

[1]孙艳，王益权，冯嘉玥. 土壤紧实胁迫对黄瓜生长、产量及养分吸收的影响. 植物营养与肥料学报，2006，12(4): 559-564.

Sun Y, Wang Y Q, Feng J Y. Effects of soil compaction stress on the growth, yields and nutrient absorption of cucumber. Plant Nutrition and Fertilizer Science, 2006, 12(4) : 559-564.(in Chinese)

[2]王群，张学林，李全忠. 紧实胁迫对不同土壤类型玉米养分吸收、分配及产量的影响. 中国农业科学，2010，43(21): 4356-4366.

Wang Q, Zhang X L, Li Q Z. Effect of soil compaction stress on nutrient absorption and distribution and yield in maize under different soil types. Scientia Agricultura Sinica, 2010, 43(21):4356-4366.(in Chinese)

[3]Ishaq M, Hassan A, Saeed M. Subsoil compaction effects on crops in Punjab, Pakistan.I.Soil physical properties and crop yield. Soil and Tillage, 2001, 59 (1-2):57-65.

[4]Ahmad N, Hassan F U, Belford R K. Effects of soil compaction in the sub-humid cropping environment in Pakistan on uptake of NPK and grain yield in wheat (Triticum aestivum): II: Alleviation. Field Crops Research, 2009, 110(1):61-68.

[5]Blouin V M, Schmidt M G, Bulmer C E, Effects of compaction and content on lodgepole pine seedling growth. Forest Ecology Management, 2008, (8):1-9.

[6]孙艳，王益权，杨梅. 土壤紧实胁迫对黄瓜根系活力和叶片光合作用的影响. 植物生理与分子生物学学报，2005，31(5): 545-550.

Sun Y, Wang Y Q, Yang M. Effects of soil compactness stress on root activity and leaf photosynthesis of cucumber. Journal of Plant Physiology and Molecular Biology, 2005, 31(5):545-550.(in Chinese)

[7]刘晚苟，山仑.不同土壤水分条件下容重对玉米生长的影响. 应用生态学报，2003，1(11)：1906-1910.

Liu W G, Shan L. Effect of soil bulk density on maize growth under different water regimes. Chinese Journal of Applied Ecology, 2003, 1 (11):1906-1910.(in Chinese)

[8]Rosolem C A, Foloni J S S, Tiritan C S. Root growth and nutrient accumulation in cover crops as affected by soil compaction. Soil Till Research, 2002, 65:109-115.

[9]贺明荣，王振林. 土壤紧实度变化对小麦籽粒产量和品质的影响. 西北植物学报，2004，24(4): 649-654.

He M R, Wang Z L. Effects of soil compaction on grain yield and quality of wheat. Acta Botanica Boreali-Occidentalia Sinica, 2004, 24(4):649-654.(in Chinese)

[10]尚庆文，孔祥波，王玉霞. 土壤紧实度对生姜植株衰老的影响. 应用生态学报，2008，19(4): 782-786.

Shang Q W, Kong X B, Wang Y X. Effects of soil compactness on ginger plant senescence. Chinese Journal of Applied Ecology, 2008, 19(4):782-786.(in Chinese)

[11]孙艳，王益权，仝瑞强. 土壤紧实胁迫对黄瓜叶片光合作用及叶绿素荧光参数的影响. 植物营养与肥料学报，2009，15(3): 638-642.

Sun Y, Wang Y Q, Tong R Q. Effects of soil compaction stress on photosynthesis, chlorophyll fluorescence parameters of cucumber leaves. Plant Nutrition and Fertilizer Science, 2009, 15(3): 638-642.(in Chinese)

[12]Hurley M B, Rowarth J S. Resistance to root growth and changes in the concentrations of ABA within the root and xylem sap during root-restriction stress. Journal of Experimental Botany, 1999, 335:799-804.

[13]Saffih-Hdadi K, Défossez P, Richard G. Chaplain a method for predicting soil susceptibility to the compation of surface layers as a function of water content and bulk density. Soil and Tillage Research, 2009, 105(1):96-103.

[14]孙艳，王益权，徐伟君. 紧实胁迫对土壤呼吸强度及黄瓜生长和品质的影响. 土壤学报，2008，45(6):1128-1134.

Sun Y, Wang Y Q, Xu W J. Effects of soil compaction on soil respiration intensity,plants growth and fruit quality of cucumber. Acta Pedologica Sinica, 2008, 45(6):1128-1134. (in Chinese)

[15]徐向东，孙艳，郭晓芹. 褪黑素对高温胁迫下黄瓜幼苗抗坏血酸代谢系统的影响. 应用生态学报，2010，21(10):2580-2586.

Xu X D, Sun Y, Guo X Q. Effect of exogenous melatonin on ascorbate metabolism system in cucumber seedlings under high temperature stress. Chinese Journal of Applied Ecology, 2010, 21(10): 2580-2586.(in Chinese)

[16]高俊杰，秦爱国，于贤昌. 低温胁迫对嫁接黄瓜叶片抗坏血酸—谷胱甘肽循环的影响. 园艺学报，2009，36(2):215 -220.

Gao J J, Qin A Q, Yu X C. Effects of low-temperature stress on the ascorbate-glutahione cycle in the grafted cucumber leaves. Acta Horticulturae Sinica, 2009, 36(2):215-220.(in Chinese)

[17]孙艳，徐伟君. 高温胁迫对不同黄瓜品种幼苗中抗坏血酸代谢的影响. 西北农业学报，2007，16(6):164-169.

Sun Y, Xu J W. Effect of high temperature stress on the metabolism of ascorbic acid in cucumber seedlings. Acta Agriculturae Boreali-occidentalis Sinica, 2007, 16(6):164-169.(in Chinese)

[18]罗娅，汤浩茹，张勇. 低温胁迫对草莓叶片SOD和AsA-GSH循环酶系统的影响. 园艺学报，2007，34(6):1405-1410.

Luo Y, Tang H R, Zhang Y. Effect of low temperature stress on activities of SOD and enzymes of ascorbate-glutathione cycle. Acta Horticulturae Sinica, 2007, 34(6):1405- 1410.(in Chinese)

[19]刘建新，王鑫，李博萍. 外源一氧化氮供体SNP对NaCl胁迫下黑麦草幼苗叶片抗坏血酸-谷胱甘肽循环的影响. 草业学报，2010，19(2)：82-88.

Liu J X, Wang X, Li B P. Effects of exogenous nitric oxide donor SNP on ascorbate??glutathione cycle metabolism in ryegrass seedling leaves under NaCl stress. Actaprat Aculture Sinica, 2010, 19(2):82-88.(in Chinese)

[20]魏国平，朱月林，刘正鲁，张古文，杨立飞. 硝酸钙胁迫对营养液栽培嫁接茄子叶片抗坏血酸-谷胱甘肽循环的影响. 植物生态学报, 2008, 32 (5):1023-1030.

Wei G P, Zhu Y L, Liu Z L, Zhang G W, Yang L F. Effects of calcium nitrate stress on ascorbate-glutathione cycle metabolism in leaves of hydroponically-grown grafted eggplant seedlings. Journal of Plant Ecology, 2008, 32 (5):1023-1030.(in Chinese)

[21]王俊力，王岩，赵天宏. 臭氧胁迫对大豆叶片抗坏血酸-谷胱甘肽循环的影响. 生态学报，2011，31(8):2068-2075.

Wang J L, Wang Y, Zhao T H. Effects of ozone on AsA-GSH cycle in soybean leaves. Acta Ecologica Sinica, 2011, 31(8):2068-2075.(in Chinese)

[22]Nevens F, Reheul D. The consequences of wheel-induced soilcompaction and subsoiling for silage maize on a sandy loamsoil in Belgium. Soil and Tillage Research, 2003, 70(2):175-184.

[23]Tubeileh A, Groleau-Renaud V, Plantureux S. Effect of soil compaction on photosynthesis and carbon partitioning within a maize-soil system. Soil and Tillage Research, 2003, 7(1):151-161.

[24]林植芳，李双顺，林桂珠. 衰老叶片和叶绿体中超氧阴离子和有机自由基浓度的变化. 植物生理学报，1988，14(1):16-22.

Lin Z F, Li S S, Lin G Z. The accumulation of hydrogen peroxide in senescing leaves and chloroplasts in relation to lipid peroxidation. Acta Photophysiologica Sinica, 1988, 14(1):16-22.(in Chinese)

[25]高俊凤.植物生理学实验指导. 西安:世界图书出版社, 2000，192-201.

Gao J F. Experimental Guidance of Plant Physiology. Xi’an: World Books Press, 2000, 192-201.(in Chinese)

[26]李潮海，赵霞，王群，郝四平. 下层土壤容重对玉米生育后期叶片衰老的生理效应. 玉米科学，2007，15(2):61-63.

Li H C, Zhao X, Wang Q, Hao S P. The physiological effect of different soil bulk densities on maize(Zea mays L.)leaf senescence during later growth stage. Journal of Maize Sciences, 2007, 15(2):61-63.(in Chinese)

[27]陆晓民，孙 锦，郭世荣，何立中.油菜素内酯对低氧胁迫黄瓜幼苗根系线粒体抗氧化系统及其细胞超微结构的影响. 园艺学报，2012,39(5): 888-896.

Lu X M, Sun J, Guo S R, He L Z. Effects of brassinolide on the mitochondria antioxidant system and cellular ultrastructure of cucumber seedling roots under hypoxic stress. Acta Horticulturae Sinica, 2012,39(5):888-896.

[28]吴亚维，马锋旺，邹养军. 土壤紧实度对楸子幼苗根系生长及活力的影响.贵州农业科学，2009，37(4):137-138.

Wu Y W, Ma F W, Zou Y J. Effects of soil compaction on photosynthetic parameters and water use efficiency of Malus hupehensis seedling. Guizhou Agricultural Sciences, 2009, 37(4): 137-138. (in Chinese)

[29]生利霞，冯立国，束怀瑞. 不同土壤紧实度对平邑甜茶根系特征及氮代谢的影响. 果树学报，2009，26(5):593-596.

Sheng L X, Feng L G, Shu H R. Effect of soil compaction on root characters and nitrogen metabolism of potted seedlings of pingyitiancha (Malus hupehensis). Journal of Fruit Science, 2009, 26(5):593-596.(in Chinese)

[30]李潮海，李胜利，王群，郝四平，韩锦峰. 下层土壤容重对玉米根系生长及吸收活力的影响. 中国农业科学，2005，38(8):1706-1711.

Li H C, Li S L, Wang Q, Hao S P, Han J F. A study on corn root growth and activities at different soil layers with special bulk density. Scientia Agricultura Sinica, 2005, 38(8):1706-1711.(in Chinese)

[31]Nagalakshmi N, Prasad M N. Reponses of glutathione cycle enzymes and glutathione metabolism to copper stress in Scenedesmus bijugatus. Plant Science, 2001, 160: 291-299.

[32]孙艳，梁宇柱，陈敬东，丁勤，徐伟君，徐向东. 黄瓜叶片衰老过程中抗坏血酸含量与生理指标关系的研究. 西北植物学报，2008，28(3): 512-516.

Sun Y, Liang Y Z, Chen J D, Ding Q, Xu W J, Xu X D. Relationships between ascorbic acid content and relative physiologic indices during senescence of cucumber leaves. Acta Botanica Boreal-Occidentalia Sinica, 2008, 28(3): 512-516.(in Chinese)

[33]樊怀福，李娟，郭世荣，杜长霞. 外源NO对NaCl胁迫下黄瓜幼苗生长和根系谷胱甘肽抗氧化酶系统的影响. 西北植物学报，2007，27(8):1611-1618.

Fan H F, Li J, Guo S R, Du C X. Effects of exogenous nitric oxide on growth and glutathione dependent antioxidative system in cucumber seedling roots under NaCl stress. Acta Botanica Boreal-Occidentalia Sinica, 2007, 27(8):1611 -1618.(in Chinese)

[34]Rao M V, Hale B A, Ormrod D P. Amelioration of ozone induced oxidative damage in wheat plants grown under high carbon dioxide. Plant Physiology, 1995, 109(2):421-432．

[35]Jin Y H, Tao D L, Hao Z Q. Environmental stresses and redox status of ascorbate. Acta Botanica Sinica, 2003, 45(7):795-801.

[36]康云艳，郭世荣，段九菊.低氧胁迫对不同耐性黄瓜品种根系抗氧化系统的影响.植物生理学通讯，2007,43(4): 630-634.

Kang Y Y, Guo S R, Duan J J. Influence of hypoxia stress on antioxidative system of roots in two different resistant cucumber (Cucumis sativus L.) cultivars. Plant Physiology Communications, 2007, 43(4): 630-634. (in Chinese)

[37]康云艳，郭世荣，李娟，段九菊. 24-表油菜素内酯对低氧胁迫下黄瓜幼苗根系抗氧化系统的影响.中国农业科学，2008, 41(1): 153-161.

Kang Y Y, Guo S R, Li J, Duan J J. Effects of 24-epibrassinolide on antioxidant system in cucumber seedlings roots under hypoxia stress. Scientia Agricultura Sinica, 2008,41(1):153-161. (in Chinese)
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